LOCUS       pAVEC1_N-TurboID_corrected        8564 bp    DNA     circular SYN 25-JUN-2025
DEFINITION  2_AVEC4_NTID_fixed_c1.
ACCESSION   2_AVEC4_NTID_fixed_c1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     CDS             827..1456
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="pVS1 StaA"
     CDS             1885..2958
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="pVS1 RepA"
     rep_origin      3024..3218
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="pVS1 oriV"
     misc_feature    3385..3408
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="95.8"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc_feature"
                     /label="LB T-DNA repeat"
     misc_feature    3430..3453
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="95.8"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc_feature"
                     /label="LB T-DNA repeat"
     terminator      complement(3545..3797)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /label="MAS terminator"
     CDS             complement(3810..4358)
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="PAT_STRHY"
     promoter        complement(4364..4744)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="MAS promoter"
     promoter        5370..5670
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="87.0"
                     /fragment="True"
                     /other="promoter"
                     /label="CaMV 35S promoter (fragment)"
     promoter        5671..6012
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="97.7"
                     /match_length="98.8"
                     /fragment="False"
                     /other="promoter"
                     /label="CaMV 35S promoter"
     misc_feature    6028..6083
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.2"
                     /match_length="101.8"
                     /fragment="False"
                     /other="misc_feature"
                     /label="TMV Ω"
     CDS             6099..7058
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="95.3"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
                     /label="BIRA_ECOLI"
     polyA_signal    7234..7393
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="91.4"
                     /fragment="True"
                     /other="polyA_signal"
                     /label="CaMV poly(A) signal (fragment)"
     CDS             7609..8418
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="KanR"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgactga agaaccgagc
      721 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
      781 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
      841 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
      901 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
      961 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1021 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1081 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1141 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1201 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1261 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1321 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1381 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1441 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1501 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1561 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1621 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     1681 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     1741 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     1801 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     1861 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     1921 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     1981 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2041 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2101 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2161 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2221 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2281 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2341 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2401 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2461 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2521 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2581 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     2641 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     2701 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     2761 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     2821 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     2881 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     2941 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3001 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3061 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3121 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3181 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3241 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3301 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3361 ccagcgcgcg gacaagctag gattggcagg atatattcag ttgtaaatgc gagctgttgg
     3421 ctggctggtg gcaggatata ttcagttgta aataaattga cgcttagaca acttaataac
     3481 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgagc tcttctagag
     3541 atctgataat ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca
     3601 atacaaagac agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga
     3661 gtaagatcac ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa
     3721 atactcaaac cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc
     3781 caacatggga gtccaagatt ctgcagtcaa atctcggtga cgggcaggac cggacggggc
     3841 ggtaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
     3901 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
     3961 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
     4021 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
     4081 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggccagcg
     4141 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
     4201 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     4261 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     4321 gcacggcgga tgtcggccgg gcgtcgttct gggctcatcg attcgatttg gtgtatcgag
     4381 attggttatg aaattcagat gctagtgtaa tgtattggta atttgggaag atataatagg
     4441 aagcaaggct atttatccat ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg
     4501 cattccgttc ttgctgtaaa gcgttgtttg gtacactttt gactagcgag gcttggcgtg
     4561 tcagcgtatc tattcaaaag tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa
     4621 cagaataccc gcgaaattca ggcccggttg ccatgtccta cacgccgaaa taaacgacca
     4681 aattagtaga aaaataaaaa ctgactcgga tacttacgtc acgtcttgcg cactgatttg
     4741 aaaaatctca gctcgaggac tagagccaag ctgatctcct ttgccccgga gatcaccatg
     4801 gacgactttc tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc
     4861 atgttcacca ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca
     4921 cagatggtta gagaggctta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat
     4981 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     5041 aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta
     5101 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag
     5161 aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca
     5221 gaactcgccg tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag
     5281 aagaaaatct tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
     5341 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
     5401 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
     5461 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
     5521 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     5581 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     5641 gatgacgcac aatcccacta tccttcgcaa tgagactttt caacaaaggg taatatcggg
     5701 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     5761 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     5821 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     5881 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     5941 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     6001 tcatttggag agaggcctat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     6061 ttacaattac tatttacaat tacaggcctc ccgggaccat gaaagataac actgttccat
     6121 tgaaactcat cgctctcctc gctaacggtg agttccactc tggtgagcag ctcggtgaga
     6181 ctttaggtat gtctcgtgct gctatcaaca agcacatcca gactttacgt gattggggag
     6241 ttgatgtttt cactgttcca ggaaagggtt actctttacc agagccaatc ccattattga
     6301 acgctaagca gatcttgggt cagcttgatg gaggatctgt tgctgttctt ccagttgttg
     6361 attctactaa ccagtacctt ctcgatcgta ttggagagct caagtctggt gatgcttgca
     6421 ttgctgagta ccagcaggca ggtcgtggtt ctcgtggtag gaagtggttc tctccattcg
     6481 gtgcaaacct ctacctttct atgttctgga ggcttaagag gggtccagct gctattggtc
     6541 ttggtccagt tattggtatt gttatggctg aggctctcag gaagctcggt gctgataagg
     6601 ttagggtgaa gtggccaaac gatctctacc tccaggatag gaagttggct ggtattttgg
     6661 tggagcttgc tggaattact ggagatgctg ctcagattgt gatcggagct ggaatcaacg
     6721 tggctatgag gagggtcgag gagtctgtcg tcaaccaggg atggatcact cttcaggagg
     6781 ctggtatcaa ccttgatagg aacacacttg ctgctacact tatcagggag cttagggctg
     6841 ctcttgagtt gttcgagcaa gagggtttgg ctccatactt gccaaggtgg gagaagttgg
     6901 ataacttcat caacaggcca gtcaagttga tcatcggtga taaggagatt ttcggtattt
     6961 ctaggggtat tgataagcaa ggtgctttgt tgttggagca agatggtgtt atcaagccat
     7021 ggatgggtgg tgagatctct ttgaggtctg ctgagaaggg gtcaggaagc gaaacaccag
     7081 gaacatcaga aagtgcaaca ccagaatctg gatccggctc tgaaactcct ggtactagtg
     7141 aatcggctac ccctgaatca gggtcctgat cacggccatg ctagagtccg caaaaatcac
     7201 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     7261 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     7321 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     7381 accaaaatcc agtgacctgg taacctggca ggatatatgc ggttgtaatt ttggcaggat
     7441 atatgcggtt gtaattcatt tttattgtct aaatttctgt atttgtttgt ttgcaattag
     7501 atccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     7561 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     7621 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     7681 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     7741 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     7801 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     7861 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     7921 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     7981 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     8041 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     8101 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     8161 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     8221 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     8281 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     8341 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     8401 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     8461 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     8521 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
