LOCUS       AVEC4__C-mNG_11              8322 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1885..2958
                     /note="pLannotate"
                     /label="pVS1 RepA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7367..8176
                     /note="pLannotate"
                     /label="KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             827..1456
                     /note="pLannotate"
                     /label="pVS1 StaA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3807..4358)
                     /note="pLannotate"
                     /label="BlpR"
                     /database="addgene"
                     /identity="99.819"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4364..4744)
                     /note="pLannotate"
                     /label="MAS promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        5671..6012
                     /note="pLannotate"
                     /label="CaMV 35S promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="99.1304347826087"
                     /fragment="False"
                     /other="promoter"
     terminator      3545..3797
                     /note="pLannotate"
                     /label="MAS terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     rep_origin      3024..3218
                     /note="pLannotate"
                     /label="pVS1 oriV"
                     /database="addgene"
                     /identity="99.49"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(6992..7151)
                     /note="pLannotate"
                     /label="CaMV poly(A) signal"
                     /database="addgene"
                     /identity="98.137"
                     /match_length="91.47727272727273"
                     /fragment="True"
                     /other="polyA signal"
     misc_feature    3385..3408
                     /note="pLannotate"
                     /label="LB T-DNA repeat"
                     /database="addgene"
                     /identity="95.833"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    3430..3453
                     /note="pLannotate"
                     /label="LB T-DNA repeat"
                     /database="addgene"
                     /identity="95.833"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc feature"
     CDS             6204..6907
                     /note="pLannotate"
                     /label="mNeonGreen"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="99.57627118644068"
                     /fragment="False"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgactga agaaccgagc
      721 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
      781 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
      841 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
      901 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
      961 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1021 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1081 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1141 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1201 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1261 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1321 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1381 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1441 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1501 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1561 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1621 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     1681 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     1741 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     1801 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     1861 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     1921 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     1981 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2041 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2101 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2161 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2221 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2281 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2341 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2401 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2461 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2521 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2581 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     2641 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     2701 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     2761 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     2821 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     2881 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     2941 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3001 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3061 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3121 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3181 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3241 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3301 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3361 ccagcgcgcg gacaagctag gattggcagg atatattcag ttgtaaatgc gagctgttgg
     3421 ctggctggtg gcaggatata ttcagttgta aataaattga cgcttagaca acttaataac
     3481 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgagc tcttctagag
     3541 atctgataat ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca
     3601 atacaaagac agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga
     3661 gtaagatcac ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa
     3721 atactcaaac cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc
     3781 caacatggga gtccaagatt ctgcagtcaa atctcggtga cgggcaggac cggacggggc
     3841 ggtaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
     3901 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
     3961 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
     4021 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
     4081 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggccagcg
     4141 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
     4201 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     4261 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     4321 gcacggcgga tgtcggccgg gcgtcgttct gggctcatcg attcgatttg gtgtatcgag
     4381 attggttatg aaattcagat gctagtgtaa tgtattggta atttgggaag atataatagg
     4441 aagcaaggct atttatccat ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg
     4501 cattccgttc ttgctgtaaa gcgttgtttg gtacactttt gactagcgag gcttggcgtg
     4561 tcagcgtatc tattcaaaag tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa
     4621 cagaataccc gcgaaattca ggcccggttg ccatgtccta cacgccgaaa taaacgacca
     4681 aattagtaga aaaataaaaa ctgactcgga tacttacgtc acgtcttgcg cactgatttg
     4741 aaaaatctca gctcgaggac tagagccaag ctgatctcct ttgccccgga gatcaccatg
     4801 gacgactttc tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc
     4861 atgttcacca ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca
     4921 cagatggtta gagaggctta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat
     4981 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     5041 aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta
     5101 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag
     5161 aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca
     5221 gaactcgccg tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag
     5281 aagaaaatct tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
     5341 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
     5401 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
     5461 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
     5521 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     5581 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     5641 gatgacgcac aatcccacta tccttcgcaa tgagactttt caacaaaggg taatatcggg
     5701 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     5761 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     5821 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     5881 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     5941 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     6001 tcatttggag agaggcctat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     6061 ttacaattac tatttacaat tacaggcctc ccgggtcagg aagcgaaaca ccaggaacat
     6121 cagaaagtgc aacaccagaa tctggatccg gctctgaaac tcctggtact agtgaatcgg
     6181 ctacccctga atcagggtcc tctgtgagta aaggtgaaga agataacatg gcgtctttgc
     6241 cagctactca cgaacttcac atcttcggtt ctatcaacgg tgttgatttc gacatggttg
     6301 gtcaaggaac tggaaaccca aacgatggat acgaagaact taaccttaag tctactaagg
     6361 gtgatcttca attctctcca tggatcttgg ttcctcacat tggttacggt ttccaccagt
     6421 acttgcctta ccctgatggt atgtctcctt tccaggctgc tatggttgac ggttctggtt
     6481 atcaggttca ccgtactatg cagttcgaag acggtgcttc tttgactgtt aactatcgtt
     6541 atacatatga aggatctcac atcaagggag aagctcaggt gaagggaaca ggattccctg
     6601 ctgacggtcc tgttatgact aactctctca ctgcagctga ttggtgtcgt tctaagaaga
     6661 cgtacccaaa tgataagacc atcatctcca ctttcaaatg gagctacact actggtaatg
     6721 gaaagaggta ccgttctact gctaggacta cttacacatt cgctaagcct atggctgcta
     6781 actacctcaa gaaccagcca atgtacgttt tcaggaagac agagttgaag cactctaaga
     6841 ctgagttgaa cttcaaggaa tggcagaagg ctttcactga tgtgatggga atggatgagc
     6901 tctacaagtg agggtcctct gtcctgatca cggccatgct agagtccgca aaaatcacca
     6961 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     7021 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     7081 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     7141 caaaatccag tgacctggta acctggcagg atatatgcgg ttgtaatttt ggcaggatat
     7201 atgcggttgt aattcatttt tattgtctaa atttctgtat ttgtttgttt gcaattagat
     7261 ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     7321 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     7381 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     7441 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     7501 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     7561 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     7621 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     7681 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     7741 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     7801 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     7861 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     7921 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     7981 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     8041 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     8101 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     8161 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     8221 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     8281 ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
