LOCUS       pAVEC2_N-TurboID_corrected        8757 bp    DNA     circular SYN 25-JUN-2025
DEFINITION  3_AVEC5_NTID_fixed_c1.
ACCESSION   3_AVEC5_NTID_fixed_c1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     CDS             827..1456
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="pVS1 StaA"
     CDS             1885..2958
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="pVS1 RepA"
     rep_origin      3024..3218
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="pVS1 oriV"
     misc_feature    3385..3408
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="95.8"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc_feature"
                     /label="LB T-DNA repeat"
     misc_feature    3430..3453
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="95.8"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc_feature"
                     /label="LB T-DNA repeat"
     terminator      complement(3542..3790)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.4"
                     /match_length="101.6"
                     /fragment="False"
                     /other="terminator"
                     /label="HSP terminator"
     CDS             complement(3795..4310)
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="P19_TBSVC"
     promoter        5563..5863
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="87.0"
                     /fragment="True"
                     /other="promoter"
                     /label="CaMV 35S promoter (fragment)"
     promoter        5864..6205
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="97.7"
                     /match_length="98.8"
                     /fragment="False"
                     /other="promoter"
                     /label="CaMV 35S promoter"
     misc_feature    6221..6276
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.2"
                     /match_length="101.8"
                     /fragment="False"
                     /other="misc_feature"
                     /label="TMV Ω"
     CDS             6292..7251
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="95.3"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
                     /label="BIRA_ECOLI"
     polyA_signal    7427..7586
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="91.4"
                     /fragment="True"
                     /other="polyA_signal"
                     /label="CaMV poly(A) signal (fragment)"
     CDS             7802..8611
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="KanR"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgactga agaaccgagc
      721 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
      781 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
      841 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
      901 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
      961 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1021 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1081 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1141 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1201 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1261 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1321 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1381 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1441 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1501 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1561 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1621 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     1681 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     1741 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     1801 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     1861 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     1921 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     1981 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2041 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2101 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2161 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2221 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2281 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2341 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2401 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2461 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2521 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2581 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     2641 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     2701 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     2761 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     2821 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     2881 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     2941 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3001 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3061 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3121 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3181 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3241 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3301 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3361 ccagcgcgcg gacaagctag gattggcagg atatattcag ttgtaaatgc gagctgttgg
     3421 ctggctggtg gcaggatata ttcagttgta aataaattga cgcttagaca acttaataac
     3481 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgagc tctctagaaa
     3541 gcttatcttt aatcatattc catagtccat accatagcac atacagtagt tatatgctgc
     3601 agaagagatc caacaaaaca ttcacaatgg attatagaaa catttgttta ttcattataa
     3661 tgagatctta cattcattta atattagaaa aagccacaaa ttcataacac aacaagccaa
     3721 gaagaaaaca caaacttaag cacacaagct ttttatttga cacaccaaat atttcatctt
     3781 catcttcata tttactcgct ttctttttcg aaggtctcag taccttcagg gcatcctctt
     3841 gatacattac tttccacttc gattggggca agctgtagca gttcttgctt agaccgaatt
     3901 gccatctcac agagatgctg aagagttcgc gaccctccag aaacggtgat actaactcct
     3961 cgaaaccgaa tactataggt acatccgatc tggtcgaaac cgaaaaatcg agatgctgca
     4021 tagttaaccg aatctcccgt ccaagatcca aggactctgt gcagtgaagc ttccgtcctg
     4081 tcgtatctga gatatctctt aaatacaact ttcccgaaac cccagctttc cttgaaacca
     4141 aggggattat cttgattcga attagtctca tcgttatgta gccgccactc agtccaactc
     4201 ggactttcgt caggaagttt gaagggagaa gtggtacctc ctgatcctcc atcccaacgt
     4261 tcactgttag cttgttccct agcgtcgttt ccttgtatag ctcgttccat ctgttaatca
     4321 gaaaaactca gattaatcga caaattcgat cgcacaaact agaaactaac accagatcta
     4381 gatagaaatc acaaatcgaa gagtaattat tcgacaaaac tcaaattatt tgaacaaatc
     4441 ggatgatatc tatgaaaccc taatcgagaa ttaagatgat atctaacgat caaacccaga
     4501 aaatcgtctt cgatctaaga ttaacagaat ctaaaccaaa gaacatatac gaaattggga
     4561 tcgaacgaaa acaaaatcga agattttgag agaataagga acacagaaat ttaccttcat
     4621 cacggtagag agaattgaga gaaagttttt aagattttga gaaattgaaa tctgaattgt
     4681 gaagaagaag agctctttgg gtattgtttt atagaagaag aagaagaaaa gacgaggacg
     4741 actaggtcac gagaaagcta aggcggtgaa gcaatagcta ataataaaat gacacgtgta
     4801 ttgagcgttg tttacacgca aagttgtttt tggctaattg ccttattttt aggttgagga
     4861 aaagtatttg tgctttgagt tgataaacac gactcgtgtg tgccggctgc aaccactttg
     4921 acgccgttta ttactgactc gtcgactcga gactagagcc aagctgatct cctttgcccc
     4981 ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt tcgacggaga
     5041 aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca atttcagaaa
     5101 gaatgctgac ccacagatgg ttagagaggc ttacgcggca ggtctcatca agacgatcta
     5161 cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5221 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5281 tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat
     5341 tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa aaattcagat
     5401 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
     5461 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tcgtctactc
     5521 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     5581 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     5641 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     5701 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5761 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5821 cactgacgta agggatgacg cacaatccca ctatccttcg caatgagact tttcaacaaa
     5881 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
     5941 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
     6001 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     6061 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     6121 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     6181 aaggaagttc atttcatttg gagagaggcc tatttttaca acaattacca acaacaacaa
     6241 acaacaaaca acattacaat tactatttac aattacaggc ctcccgggac catgaaagat
     6301 aacactgttc cattgaaact catcgctctc ctcgctaacg gtgagttcca ctctggtgag
     6361 cagctcggtg agactttagg tatgtctcgt gctgctatca acaagcacat ccagacttta
     6421 cgtgattggg gagttgatgt tttcactgtt ccaggaaagg gttactcttt accagagcca
     6481 atcccattat tgaacgctaa gcagatcttg ggtcagcttg atggaggatc tgttgctgtt
     6541 cttccagttg ttgattctac taaccagtac cttctcgatc gtattggaga gctcaagtct
     6601 ggtgatgctt gcattgctga gtaccagcag gcaggtcgtg gttctcgtgg taggaagtgg
     6661 ttctctccat tcggtgcaaa cctctacctt tctatgttct ggaggcttaa gaggggtcca
     6721 gctgctattg gtcttggtcc agttattggt attgttatgg ctgaggctct caggaagctc
     6781 ggtgctgata aggttagggt gaagtggcca aacgatctct acctccagga taggaagttg
     6841 gctggtattt tggtggagct tgctggaatt actggagatg ctgctcagat tgtgatcgga
     6901 gctggaatca acgtggctat gaggagggtc gaggagtctg tcgtcaacca gggatggatc
     6961 actcttcagg aggctggtat caaccttgat aggaacacac ttgctgctac acttatcagg
     7021 gagcttaggg ctgctcttga gttgttcgag caagagggtt tggctccata cttgccaagg
     7081 tgggagaagt tggataactt catcaacagg ccagtcaagt tgatcatcgg tgataaggag
     7141 attttcggta tttctagggg tattgataag caaggtgctt tgttgttgga gcaagatggt
     7201 gttatcaagc catggatggg tggtgagatc tctttgaggt ctgctgagaa ggggtcagga
     7261 agcgaaacac caggaacatc agaaagtgca acaccagaat ctggatccgg ctctgaaact
     7321 cctggtacta gtgaatcggc tacccctgaa tcagggtcct gatcacggcc atgctagagt
     7381 ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa
     7441 tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg
     7501 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     7561 tctaattcct aaaaccaaaa tccagtgacc tggtaacctg gcaggatata tgcggttgta
     7621 attttggcag gatatatgcg gttgtaattc atttttattg tctaaatttc tgtatttgtt
     7681 tgtttgcaat tagatccacg ttgtgtctca aaatctctga tgttacattg cacaagataa
     7741 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     7801 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     7861 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     7921 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     7981 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     8041 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     8101 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     8161 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     8221 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     8281 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     8341 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     8401 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     8461 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     8521 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     8581 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     8641 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     8701 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
