LOCUS       B8-pVTCB052             2940 bp    DNA     circular UNA 27-NOV-2024
DEFINITION  .
ACCESSION   urn.local...6f4-hmr77qj
VERSION     urn.local...6f4-hmr77qj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      1..588
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (pVTCB052 (add RBS) - ori)"
                     /label="ori"
     terminator      600..627
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - T7Te terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7Te
                     terminator (pVTCB052 (add RBS) - T7Te terminator)"
                     /label="T7Te terminator"
     CDS             complement(651..1511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="This feature has 2 segments: 1: 1728 .. 2519 /
                     #ccffcc 2: 2520 .. 2588 / #ccffcc / signal sequence
                     Cleavage site after base 2519"
                     /Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK
                     DAEDQLGARVGYIELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTA
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEADKVAGSLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS
                     QATMDERNRQIAEIGASLIKHW"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (pVTCB052 (add RBS) - AmpR)"
                     /label="AmpR"
     promoter        complement(1512..1614)
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - cat promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cat
                     promoter (pVTCB052 (add RBS) - cat promoter)"
                     /label="cat promoter"
     terminator      1615..1646
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - tonB terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tonB
                     terminator (pVTCB052 (add RBS) - tonB terminator)"
                     /label="tonB terminator"
     promoter        1766..1819
                     /standard_name="Bba_R0040 TetR Promoter"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNP561 (reversed)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (<a
                     href=""urn:local:.:ak-hgsb63n"">pNP561 (reversed)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (pVTCB050 v1 - Bba_R0040 TetR
                     Promoter)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (pVTCB050 v1 - part 1)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (<a
                     href=""urn:local:.:je-hgxlwdf"">pVTCB050 v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (<a
                     href=""urn:local:.:c3-hgsbtku"">pNP568-pNP566_Recombinatio
                     n_Product_(reversed)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (<a
                     href=""urn:local:.:4z-hgs9xym"">p2 gg site</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (<a
                     href=""urn:local:.:fi-hgscbxs"">pVTCB050 v1</a>)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - Bba_R0040 TetR
                     Promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Bba_R0040 TetR Promoter (pVTCB052 (add RBS) - Bba_R0040
                     TetR Promoter)"
     misc_feature    1825..1849
                     /created_by="vincenttran"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - RBS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RBS
                     (pVTCB052 (add RBS) - RBS)"
                     /label="RBS"
     CDS             1850..2539
                     /created_by="vincenttran"
                     /label="rd 2 plate 2 b8"
     terminator      2562..2694
                     /standard_name="BBa_B0015 Terminator"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pVTCB050 v1 - BBa_B0015 Terminator"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     BBa_B0015 Terminator (pVTCB050 v1 - BBa_B0015 Terminator)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     BBa_B0015 Terminator (<a
                     href=""urn:local:.:c6-hgsbyhn"">p3 terminator</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     BBa_B0015 Terminator (<a
                     href=""urn:local:.:je-hgxlwdf"">pVTCB050 v2</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     BBa_B0015 Terminator (<a
                     href=""urn:local:.:c3-hgsbtku"">pNP568-pNP566_Recombinatio
                     n_Product_(reversed)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     BBa_B0015 Terminator (<a
                     href=""urn:local:.:fi-hgscbxs"">pVTCB050 v1</a>)"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:-122/0;BBa_B0015 Terminator (pVTCB050
                     v1 - Ligation)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/123;
                     BBa_B0015 Terminator (pVTCB050 v1 - Ligation)"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - BBa_B0015
                     Terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     BBa_B0015 Terminator (pVTCB052 (add RBS) - BBa_B0015
                     Terminator)"
     terminator      complement(2657..2684)
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - T7Te terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7Te
                     terminator (pVTCB052 (add RBS) - T7Te terminator)"
                     /label="T7Te terminator"
     terminator      2890..2919
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
                     /note="Derived using Geneious Prime 2024.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVTCB052 (add RBS) - T3Te terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T3Te
                     terminator (pVTCB052 (add RBS) - T3Te terminator)"
                     /label="T3Te terminator"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaggt aactggcttc
      121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcatcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcagaaaggc ccacccgaag gtgagccagg tgattacatt tgggccctca ttaccaatgc
      661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      721 ctccccgtcg tgtagataac tacgatgcgg gagggcttac catctggccc cagtgctgca
      781 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      841 gggagggccg agcgcagaag tgatcctgca actttatccg cctccatcca gtctattaat
      901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1081 ttcggtcctc cgatcgttgc cagaagtaag ttggccgcag tgttatcact catggttatg
     1141 gcagcactgc ataattctct tactgtcatg ccatccgtga gatgcttttc tgtgactggt
     1201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1321 aaacgttctt cggggcgtaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1501 tgaatactca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     1561 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaagtcaa
     1621 aagcctccgg tcggaggctt ttgactttct gctatggagg tcaggtatga tttaaatggt
     1681 cagtattgag cgatatctag agaattcgta taggttgtcg gatagcgcgg aataggattg
     1741 tgccctctga gcggataaca atttctccct atcagtgata gagattgaca tccctatcag
     1801 tgatagagat actgagcact acctggatct gaaagaggag aaatgatcta tgtctaaagt
     1861 agaggaactg attaaacccg aaatgaaaat gaaactggaa atggaaggcg aggtgaatgg
     1921 tcacaagttc agtattgagg cagaaggcga aggtaagcca tacgaaggca aacaaacgat
     1981 taaggcctgg tccacgacgg gcaaactgcc gtttgcttgg gacatcttaa gcaccagcct
     2041 gacctatggc tttcgcatgt tcacgaagta tcctgaaggc ttagaagaac atgattattt
     2101 caagcagtct tttccggaag gttacagctg ggaacgcacg attacctatg aagatggtgc
     2161 gactgttaag gtgacttcag acatctcgct tgaggacggc gtgctgatca acaaaatcaa
     2221 atttaaaggc actaatttcc cgtcagacgg accggtgatg cagaaaaaaa ctacgggatg
     2281 ggagcctagt actgaattaa ttactcccga tccggccaca ggtggtctga aaggcgaagt
     2341 taaaatgcgt ctgaaactgg agggtggtgg ccacctgctg gcggatttca agacgaccta
     2401 ccgtagcaaa aagaaagaga agctgccgct cccgggcgta cactatgtcg atcataccat
     2461 tcgtaacgaa aaagctccac acccggaagg taaagagtat gtcgtgcagt atgaaaccgc
     2521 ggtcgcccgt ctggcataat gaaggcaagc ttatcgatga tccaggcatc aaataaaacg
     2581 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2641 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tagaaagctg
     2701 tcaaacatga gaattacaac ttatatcgta tggggctgac ttcaggtgct acatttgaag
     2761 agataaattg cactgaaatc tagaaatatt ttatctgatt aataagatga tcacgataac
     2821 gaattcaagc ttgatatcat tcaggacgag cctcagactc cagcgtaact ggactgcaat
     2881 caactcactg gctcaccttc acgggtgggc ctttcttcgg tagaaaatca aaggatcttc
//
