LOCUS       pAAV-GFAP-CAS9          7700 bp ds-DNA     circular     18-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    100..645
                     /label="GFAP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    661..4857
                     /label="Cas9"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
       61 tggccaactc catcactagg ggttcctgcg gcctctagat ctgcaagcag acctggcagc
      121 attgggctgg ccgcccccca gggcctcctc ttcatgccca gtgaatgact caccttggca
      181 cagacacaat gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca
      241 aatgccttcc gagaagccca ttgagcaggg ggcttgcatt gcaccccagc ctgacagcct
      301 ggcatcttgg gataaaagca gcacagcccc ctaggggctg cccttgctgt gtggcgccac
      361 cggcggtgga gaacaaggct ctattcagcc tgtgcccagg aaaggggatc aggggatgcc
      421 caggcatgga cagtgggtgg caggggggga gaggagggct gtctgcttcc cagaagtcca
      481 aggacacaaa tgggtgaggg gagagctctc cccatagctg ggctgcggcc caaccccacc
      541 ccctcaggct atgccagggg gtgttgccag gggcacccgg gcatcgccag tctagcccac
      601 tccttcataa agccctcgca tcccaggagc gagcagagcc agagcaccgg tgccaccatg
      661 tacccatacg atgttccaga ttacgcttcg ccgaagaaaa agcgcaaggt cgaagcgtcc
      721 gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc
      781 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
      841 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
      901 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
      961 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     1021 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     1081 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     1141 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     1201 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     1261 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     1321 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     1381 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     1441 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     1501 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     1561 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     1621 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     1681 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     1741 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     1801 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     1861 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     1921 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     1981 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     2041 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     2101 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     2161 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     2221 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     2281 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     2341 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     2401 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     2461 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     2521 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     2581 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     2641 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     2701 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     2761 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     2821 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     2881 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     2941 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     3001 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     3061 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     3121 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     3181 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     3241 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     3301 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     3361 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     3421 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     3481 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     3541 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     3601 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     3661 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     3721 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     3781 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     3841 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     3901 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     3961 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     4021 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     4081 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     4141 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     4201 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     4261 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     4321 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     4381 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     4441 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     4501 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     4561 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     4621 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     4681 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     4741 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     4801 ctgtctcagc tgggaggcga cagccccaag aagaagagaa aggtggaggc cagctaagaa
     4861 ttcaataaaa gatctttatt ttcattagat ctgtgtgttg gttttttgtg tgcggccgca
     4921 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     4981 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     5041 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     5101 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     5161 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     5221 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5281 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5341 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5401 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5461 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     5521 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5581 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5641 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5701 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5761 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5821 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5881 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5941 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6001 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6061 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6121 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6181 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6241 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6301 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6361 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6421 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6481 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6541 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6601 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6661 attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg
     6721 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6781 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6841 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6901 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7621 cggttcctgg ccttttgctg gccttttgct cacatgtcct gcaggcagct gcgcgctcgc
     7681 tcgctcactg aggccgcccg
//