LOCUS       Exported                5039 bp ds-DNA     circular SYN 17-NOV-2024
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
REFERENCE   1  (bases 1 to 5039)
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(1..105)
                     /label=T1 terminator
     CDS             complement(179..1261)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1262..1339)
                     /gene="lacI"
     misc_feature    1395..1448
                     /label=pLac_promoter
     misc_feature    1397..1413
                     /label=lac operator
     misc_feature    1420..1436
                     /label=lac operator
     misc_feature    1452..1471
                     /label=sgRNA-1
                     /note="GC50b2-sg2"
     misc_feature    1472..1513
                     /label=handle
     misc_feature    1514..1551
                     /label=term
     terminator      complement(1608..1710)
                     /label=T0 terminator
     CDS             1871..2731
                     /label=AmpR
                     /note="Ap"
     misc_feature    complement(2804..3008)
                     /label=oriT
     rep_origin      3049..3668
                     /label=pBR322_origin
ORIGIN
        1 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
       61 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctttaa ttaaagcgga
      121 taacaatttc acacaggagg ccgcctaggc cgcggccgcg cgatagaatt atgacaactc
      181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      241 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
      301 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
      361 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
      421 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
      481 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
      541 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
      601 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
      661 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
      721 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
      781 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
      841 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
      901 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
      961 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     1021 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     1081 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     1141 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     1201 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     1261 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     1321 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
     1381 aagcagccct agaaaattgt gagcggataa caattgacat tgtgagcgga taacaagata
     1441 ctgagcacat aatttccgtg cctgggtatg agttttagag ctagaaatag caagttaaaa
     1501 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tctgcaggca
     1561 tgcaagcttg cggccgcgtc gtgactggga aaaccctggc gactagtctt ggactcctgt
     1621 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     1681 gccgggcgtt ttttattggt gagaatccag gggtccccaa taattacgat ttaaattagt
     1741 agcccgccta atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca
     1801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1861 aaggaagagt atgagcattc agcattttcg tgtggcgctg attccgtttt ttgcggcgtt
     1921 ttgcctgccg gtgtttgcgc atccggaaac cctggtgaaa gtgaaagatg cggaagatca
     1981 actgggtgcg cgcgtgggct atattgaact ggatctgaac agcggcaaaa ttctggaatc
     2041 ttttcgtccg gaagaacgtt ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc
     2101 ggttctgagc cgtgtggatg cgggccagga acaactgggc cgtcgtattc attatagcca
     2161 gaacgatctg gtggaatata gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt
     2221 gcgtgaactg tgcagcgcgg cgattaccat gagcgataac accgcggcga acctgctgct
     2281 gacgaccatt ggcggtccga aagaactgac cgcgtttctg cataacatgg gcgatcatgt
     2341 gacccgtctg gatcgttggg aaccggaact gaacgaagcg attccgaacg atgaacgtga
     2401 taccaccatg ccggcagcaa tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct
     2461 gaccctggca agccgccagc aactgattga ttggatggaa gcggataaag tggcgggtcc
     2521 gctgctgcgt agcgcgctgc cggctggctg gtttattgcg gataaaagcg gtgcgggcga
     2581 acgtggcagc cgtggcatta ttgcggcgct gggcccggat ggtaaaccga gccgtattgt
     2641 ggtgatttat accaccggca gccaggcgac gatggatgaa cgtaaccgtc agattgcgga
     2701 aattggcgcg agcctgatta aacattggta aaccgataca attaaaggct ccttttggag
     2761 cctttttttt tggacgaccc ttgtcctttt ccgctgcata accctgcttc ggggtcatta
     2821 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     2881 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     2941 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     3001 acgggaatcc tgctctgcga ggctggccgt aggccggccc cgtagaaaag atcaaaggat
     3061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3181 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3721 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3781 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3841 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     3901 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     3961 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4021 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4081 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     4141 gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     4201 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     4261 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     4321 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     4381 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggggcgcg agattgattt
     4441 accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa
     4501 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     4561 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gcccgagata
     4621 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     4681 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa
     4741 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     4801 cgatttagag cttgacgggg aaagcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4861 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4921 ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat cctttttgat
     4981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgaggcgc gcccagctg
//
