LOCUS       Exported                8144 bp ds-DNA     circular SYN 20-NOV-2024
DEFINITION  synthetic circular DNA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8144)
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    12..47
                     /label=GFP promoter
     misc_feature    12..31
                     /label=Target Site (iA)
     misc_feature    32..40
                     /label=(-10)
     CDS             77..799
                     /codon_start=1
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP"
     terminator      803..850
                     /label=T7 terminator SBa_000587
     terminator      857..908
                     /label=PheA-1 terminator
     misc_feature    926..956
                     /label=dCas9 promoter
     CDS             994..5100
                     /codon_start=1
                     /label=dCas9
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      complement(5160..5262)
     CDS             5494..6285
                     /label=SpecR/StrepR
     misc_feature    complement(6314..6518)
                     /label=OriT
     rep_origin      6550..8023
                     /label=PSC101
     terminator      complement(8056..8122)
                     /label=T1
ORIGIN
        1 taattaatag ctttacaccc tagcgtagct ttggtattat gctagcttct agacaattaa
       61 tagggaggtt ctcgagatgc gtaaaggcga agaactgttt accggtgtgg ttccgattct
      121 ggtggaactg gacggcgatg ttaatggtca taaattcagt gttcgcggcg aaggtgaagg
      181 cgatgcgacg aacggcaaac tgaccctgaa atttatctgc accacgggta aactgccggt
      241 cccgtggccg acgctggtga ccacgctgac ctatggcgtt caatgttttg cgcgttaccc
      301 ggatcacatg aaacagcacg actttttcaa atcggccatg ccggaaggct atgtgcagga
      361 acgtacgatt agctttaaag acgatggtac gtataaaacc cgcgcggaag tgaaattcga
      421 aggcgatacc ctggttaacc gtatcgaact gaaaggtatc gatttcaaag aagacggcaa
      481 tattctgggt cataaactgg aatataactt caattcccac aacgtgtaca tcaccgcgga
      541 taaacagaaa aacggcatta aagccaattt caaaatccgc cataatgtgg aagatggtag
      601 cgttcagctg gccgaccact atcagcaaaa cacgccgatt ggtgatggcc cggtcctgct
      661 gccggacaat cactacctga gtacccagtc cgtgctgtca aaagatccga acgaaaaacg
      721 tgaccacatg gtcctgctgg aatttgtgac ggctgcgggt atcacccacg gcatggacga
      781 actgtataaa aggccttaat aatactcgaa cccctagccc gctcttatcg ggcggctagg
      841 ggttttttgt cctagagacg aacaataagg cctcccaaat cggggggcct tttttattga
      901 taacaaaacc taggccaggc atcaattgaa atcatcaaac tcattatgga tttaatttaa
      961 actcaaggga catcaggagg agggaattct agaatggata agaaatactc aataggctta
     1021 gctatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct
     1081 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg
     1141 gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt
     1201 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag
     1261 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa
     1321 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat
     1381 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg
     1441 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg
     1501 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta
     1561 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa
     1621 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc
     1681 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc
     1741 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat
     1801 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg
     1861 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat
     1921 actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat
     1981 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa
     2041 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa
     2101 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta
     2161 ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct
     2221 attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt
     2281 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct
     2341 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct
     2401 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa
     2461 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca
     2521 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat
     2581 gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt
     2641 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc
     2701 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct
     2761 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat
     2821 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg
     2881 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa
     2941 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt
     3001 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc
     3061 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa
     3121 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt
     3181 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa
     3241 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca
     3301 actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa
     3361 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa
     3421 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat
     3481 attaatcgtt taagtgatta tgatgtcgat gccattgttc cacaaagttt ccttaaagac
     3541 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac
     3601 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc
     3661 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt
     3721 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag
     3781 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt
     3841 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat
     3901 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta
     3961 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc
     4021 tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata
     4081 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa
     4141 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact
     4201 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg
     4261 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca
     4321 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa
     4381 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg
     4441 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg
     4501 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa
     4561 gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt
     4621 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca
     4681 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca
     4741 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt
     4801 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa
     4861 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt
     4921 attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca
     4981 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat
     5041 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactga
     5101 taactgcagg catgcaagct tgcggccgcg tcgtgactgg gaaaaccctg gcgactagtc
     5161 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     5221 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
     5281 atttacgtat ttaaatgaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     5341 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     5401 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     5461 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
     5521 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     5581 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     5641 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     5701 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     5761 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     5821 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     5881 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     5941 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     6001 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     6061 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     6121 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     6181 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     6241 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataagacaa ttgtcctttt
     6301 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     6361 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     6421 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     6481 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt
     6541 aggccggcct ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct
     6601 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg
     6661 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     6721 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     6781 ttgcgtgagc catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa
     6841 ttttgcctca aaactggtga gctgaatttt tgctgttaaa gcatcgtgta gtgtttttct
     6901 tagtccgtta tgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     6961 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     7021 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     7081 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     7141 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     7201 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     7261 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     7321 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     7381 atagtttgtc cactggaaga tcttaaagcc tttaaccaaa ggattcctga tttccacagt
     7441 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     7501 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     7561 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     7621 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     7681 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     7741 ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     7801 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     7861 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     7921 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     7981 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga cctggcgcgc ccagctgtct
     8041 agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     8101 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctt
//
