LOCUS       rag2-myr-mAkt2_p       11777 bp    DNA     circular SYN 30-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2024 from SnapGene 8.0.1
            https://www.snapgene.com
COMMENT     Sequence Label: rag2-myr-mAkt2/pISceI
FEATURES             Location/Qualifiers
     source          1..11777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..6505
                     /label=zebrafish rag2 promoter
                     /label=zrag2 promoter
     misc_feature    6545..8074
                     /label=myr-mAkt2
     CDS             6545..6631
                     /label=myr-HA
     misc_feature    6632..8074
                     /label=mAkt2
     polyA_signal    8208..8338
                     /label=SV40 pA
     misc_signal     complement(8478..8495)
                     /label=I-SceI (Rev)
     misc_feature    9298..10158
                     /label=Ampicillin-R
                     /label=nonstandard type: misc. marker
     rep_origin      complement(10303..10976)
                     /direction=LEFT
                     /label=Ori
     misc_signal     complement(11391..11408)
                     /label=I-SceI (Rev)
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgccccag tataaaccag caggtcaacc tgtagtctgt gtgggtccta atgccccagt
      961 ataaaccagc aggtctacct gtggtctgtg tgggtcctaa tgccccagta taaaccaaca
     1021 ggtcaacctg tgatctgtgt gggtcctaat gccccagtat aaaccagcag gtcaacctgt
     1081 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1141 gtcctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg tcctaatgcc
     1201 ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc cagtataaac
     1261 cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa
     1321 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg
     1381 tgtgggtcct aatgccccag tataaaccag caggtcaacc tgtggtctgt gtgggtccta
     1441 atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta
     1501 taaaccagca ggtcaacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
     1561 gtctacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg
     1621 gtctgtgtgg gtcctaatgc cccagtataa accagcaggt caacctgtgg tctgtgtggg
     1681 tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc
     1741 cagtataaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc
     1801 agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac
     1861 ctgtggtctg tgtgggtcct aatgccccag tataaaccag caggtcaacc tgtggtctgt
     1921 gtgggtccta atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa
     1981 tgccccagta taaaccagca gatcaacctg tggtctgtgt gggtcctaat gccccagtat
     2041 aaaccagcag atcaacctgt ggtctgtgtg ggtcctaaag tcccaggata aaccagcatg
     2101 tcaacctgtg gtctgtgtgg gtcctaatgc cccggtataa accagcagat caacctgtgg
     2161 tctgtgtggg tcctaatgcc ccaggataaa ccagcaggtc aacctgtggt ctgtgtgggt
     2221 cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc
     2281 agtatattga tggagactct atactgctcg gtgagctcca tctttcggat aagatgttaa
     2341 aacaaggtcc cgactctctg tggtggtaaa aaattcccag gatgtccttc ctaccctggc
     2401 atcctggaca aatttgctca ctgtcctctg tccatcatgg cctcctaacc atccccatat
     2461 cataattggc tttatcactc tagctggggc cgaaatcgca tacttccatt ttatgaggta
     2521 cgctaaaatc agtatgcgag ccaagtaata tgtccgaata cttccggcga gattctagag
     2581 tgtgcattcg atgcacgcta cgctattcca tgatgccccg tgagagaatt cttgaatcgg
     2641 agagaagcaa cgcaactcat gcgggtaggt tacgtgacga tgacatattg gcggatgtag
     2701 tacgtccaag ttccattcat actgctcaca ttcatatttt atagaatgtt cttttcccac
     2761 agccaagtag taagtttaaa ttcaaataca gtacctacta agtagtagta gacggtttcg
     2821 gacgcagccc ctgtctcctc tccaccaata tactggtgtg tagtgtgcgg tctggtgcaa
     2881 tatggctgcc atcgcgtcat ccaggtggat gctgcacact ggtggttgat gaggagattt
     2941 tgcccaaaaa agtgttttga gtttccagaa aagcgcgata taaatgtaag gaattatcat
     3001 tagcttatta taatggaact ttctggataa cttgtgccag agctaggggc cattaaaact
     3061 ttgccctggg gtacctcagg gagctgcaag gaagtgctat agggctaatg aaatgtttgg
     3121 gggaaaaaaa aaaattttat aagtaaaaat aaaactaaaa taattaataa tttcattgtt
     3181 gaaaattata gtaaaattga taaacaagat aagcagcatt agtataaaaa actaaaaaca
     3241 aacgtaattt aaaataatta aattgtttta aataaattat acaaaataat gtttacaacg
     3301 acacggtggt gcagtaggta gtgctgtcgc cagcaaagca agaaggtcgc tggttcgagc
     3361 ctcagctgga tcacttgatg tttctgtgtg gagcattcgc gtgcgtttaa ccccagatta
     3421 ataaagagac taagccgaaa agaaaataaa tgtatgaaaa tgtttacgta aaatgtaaat
     3481 aaatgcatga ttaataaaat atatgaattt taaagattga ggtagaattg ttttattatt
     3541 cacacatttg gttattttta atcagtgaca acttgtgatg ctctttatat tgtttattac
     3601 gctactttga atattcagtc tcaaattgca tgtaaatacg acttcaaaac agaattagtt
     3661 taatttaatt gcctgtaaac agtgttgtac tttgatggtg attgtctgat aataatatga
     3721 tttcactatt taaaatttga aaaaaaaaaa tctgaaaaat actcaccggc cactttatta
     3781 ggtacacctg tctaactgct tgttaacgta aatttctaat cagccaatca ccaggcagca
     3841 actcagtgca tttaggcatg tagacatggt caagacgatc tgctgcagtt caaagcgagc
     3901 atcagaatgg agaagcaagg tgatttaagt gactttaaac gtgacgtgtt tgttggtgcc
     3961 agacgggctg gtctgagtat ttcagaaact gctgatttac tggggttttc acacacaaac
     4021 atctctgggg tttacagaga atggtccaaa aaaagataat atccagtgag cggcagttct
     4081 gtgggggcaa atgtcttgtt gatgccagag gtcagaggag aatggcctga ctggttccag
     4141 ctgatagaaa ggcaacagta attcaaataa ccactcgtta gaactgaggc atgcagaaga
     4201 gcacctttga acacaacatg tcgaaccttg aggcgggtgg gctacagcag ctcaagacca
     4261 catcgggtgc cactcctgtc agctaagaac aggaaactga ggctacaatt cgcacaggct
     4321 caacataagt agacaataga agattgaaca ttatgttgcc tggtcagatg gatacattct
     4381 accttgtatc aacagttcag gctggtgatg gtggtgtaat ggtgtggggg atatattctt
     4441 ggcacacttt gggcccatta gtaccaatta accatcgtgt caacgccaca gcctaactga
     4501 gtattgttgc tgaccatgtt catttctttt tgaccacagt gtacccatct tctgatggct
     4561 acttcctgca ggataatgca ccatgtcata aagcgtgaat gatctcagac tggtttcttg
     4621 aacatgacaa tgagttcact gtgctcgaat gacctccaca gtcaccagat ctcaatccaa
     4681 tagagcgcct ttggggtgtg gtagaatgga agattcgcat cctggatgtg cagccgacaa
     4741 atctgcatga tgctagcatg tcaatatgga ccaaaatctc tgaggaattt tccagtacct
     4801 ggttgaaatg caaaaggaag ggaatctatg ctaaggcaaa aagaggtcca acccagtact
     4861 agtggggtgt acataataaa gtggccggtg agtgtatatt gttaaggata tctagataat
     4921 ccaaataatt tacagcacta tttaaaaaaa aaaactttat gtcaccaagt ctgcatttat
     4981 ttgatcacaa ttacagtcga aaaagcaata tcgggataca ttgtttccat ttaaaataac
     5041 tatattatat tttaaatatt tttgacaatg ttatttcttt aatgccaaag ctaaattgta
     5101 ttcaatctga attttagtgt cacatgatcc ttcagaaaat attaacacat tgctaaaaat
     5161 gtatattatt atcaatttgg attttttttt ttttttgtat attaacctat gctattggtt
     5221 actatacaaa agtattactg tgaatatatg catatacaga accgtatcaa gcgcgggggg
     5281 tggttgggtt atgtccccat ttggaaagac ctggaaacat tcataatgac taaaattgca
     5341 cggaaaaata taatatctat gagtattgct gatcagcagc acaaaaccag aactccatat
     5401 taataaacag ccaatgatgc agtgagtttg tgcatgtttt cattcaaact cgtgtgattg
     5461 tgctcaaaca cctgttgtga gcagcagttg caaacagtaa acctcaagag gagccagtca
     5521 accttcacaa acactcaacc tctggctcac tcactaaaca attcgcgtta atatctaaaa
     5581 taaaaatttc cagcaccaaa cagctctcag atttcggagt acactgacca agaggtaaga
     5641 agtaacgtta cattactagg acgactattg taagactaga tatgtaatat ttggaagcgt
     5701 acgtgtttta agtgacatat agagtagagg aactgatgga aatacgattt gcataatgta
     5761 aaatgatata tattaaaata taggctacag taggtaacgt ggtttacata taacgttact
     5821 gtgttaatgt agtgctcagt tactcgtgtt gacagttatt attagtgctt gctaatttgt
     5881 ttgttaatgc ttctcttgcg tatttgatta ccttcaggcg aatctaaaac atatcagatg
     5941 tagaaacggc agaaaaatgt tgaagaaaat acgagaatag tagcttttgg agcacataga
     6001 aaagtacatc agataaagcc taccgtttaa acgcagacgc cgctctcgca ctgaatgctg
     6061 ggtactgtag tccttatatt gtatgcttaa tgtgcgtctc aacgggaaac tagagttagg
     6121 aaaactacaa tttcctgtgg aaaatgtttc tgttttcggg gaaacagcga aagaaagata
     6181 tggttccgcg tttttttctt actttgaata ataaaataag acctaactac acacttcgct
     6241 tactcttatg ctaatttgaa gcactagcat tctttgatac aatgataatc atttaaacat
     6301 cacagttata ctacaaatac tatggttaaa ctatgtctag tgtagtgtaa cactatggtt
     6361 gccattgtta tctttaagtt taagtattat ctgtggtaaa gccatggttg gtttttataa
     6421 gaggtaataa aattaacatt gtacaattaa atgactataa attaatgttt ttttttattt
     6481 tttttacaca gctaccttca aaaagggatc ccatcgattc gaattcaagg agcttgccgc
     6541 caccatgggg agcagcaaga gcaagcccaa ggaccccagc cagcgcgggg gagaattcat
     6601 gtacccttat gatgtgccag attatgcctc taatgaggta tctgtcatca aagaaggctg
     6661 gctccacaaa cgtggtgaat acatcaagac ctggaggcca cggtacttcc ttctgaagag
     6721 tgatggatct ttcattgggt ataaggagag gcccgaggcc cctgaccaga ccttaccccc
     6781 cctgaacaat ttctctgtag cagaatgcca gctgatgaag actgagaggc cacgacccaa
     6841 cacctttgtc atacgctgcc tgcagtggac cacagtcatc gagaggacct tccatgtaga
     6901 ctctccagat gagagggaag agtggatgcg ggctatccag atggtcgcca acagtctgaa
     6961 gcagcggggc ccaggtgagg acgccatgga ttacaagtgt ggctccccca gtgactcttc
     7021 cacatctgag atgatggagg tagctgtcaa caaggcacgg gccaaagtga ccatgaatga
     7081 cttcgattat ctcaaactcc tcggcaaggg caccttcggc aaggtcattc tggttcgaga
     7141 gaaggccact ggccgctatt atgccatgaa gatcctgcgc aaggaggtca tcattgcaaa
     7201 ggatgaagtc gcccacacag tcacagagag ccgggttctg cagaatacca ggcacccctt
     7261 ccttacagcc ctcaagtatg ccttccagac ccatgaccgc ctatgctttg tgatggagta
     7321 tgccaacggg ggtgagctgt ttttccacct ctctcgggag cgagtcttca cggaggatcg
     7381 ggcgcgcttt tatggagcag agattgtgtc agctctggag tatttgcact cgagagatgt
     7441 ggtgtaccgt gacatcaagc tggaaaacct tatgttggac aaagatggcc acatcaagat
     7501 cactgacttt ggcttgtgca aagagggcat cagtgatgga gccaccatga aaaccttctg
     7561 tggtaccccg gagtacttgg cgcctgaggt gctagaggac aatgactatg ggcgagcagt
     7621 ggactggtgg gggctgggtg tggtcatgta tgagatgatg tgtggccgcc tgccattcta
     7681 caaccaggac cacgagcgcc tctttgagct cattcttatg gaggagatcc gcttcccgcg
     7741 cacactcggg ccagaggcca agtccctgct ggctggactg ctgaagaagg acccaaagca
     7801 gaggctcggc ggaggtccca gtgatgcgaa ggaggtcatg gagcatagat tcttcctcag
     7861 catcaactgg caggacgtgg tacagaaaaa gctcctgcca cccttcaaac ctcaggtcac
     7921 ttcagaagtg gacacaaggt actttgatga cgagttcacc gcccagtcca tcacaatcac
     7981 acccccagac cgatatgaca gcctggaccc gctggaactg gaccagcgga cgcacttccc
     8041 ccagttctcc tactcagcca gcatccgaga gtgagcagcc ctctgccacc acaggacaca
     8101 agcatggccg tcatccactg cctgggtggc tttttaaaaa aaaaaaaaaa aaggaattct
     8161 gcagatcctc tcgagcctag cgctaccgga gcttatcgat gatgatccag acatgataag
     8221 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     8281 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     8341 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     8401 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag atcagatcca
     8461 ctagttctag agcggccatt accctgttat ccctaggccg ccaccgcggt ggagctccaa
     8521 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     8581 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8641 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8701 tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     8761 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     8821 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     8881 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     8941 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     9001 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9061 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9121 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     9181 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9301 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9361 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9421 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9481 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9541 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9601 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9661 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9721 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9781 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9841 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9901 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9961 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10021 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10081 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10141 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10201 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10441 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10981 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11041 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    11101 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    11161 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    11221 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    11281 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
    11341 attaaccctc actaaaggga acaaaagctg ggtaccgggc cccccctcga attaccctgt
    11401 tatccctatc gagcaaaatc aatcctaatg taaaatagta tacgttcatc attataatgc
    11461 aaacactgta gaactgttgt gttgtgcctt gcatttcatt ctcagtttct tatcgtctgt
    11521 ctctttttac gacagagcta gaaaaaacta gccttcattt gccatcgccg tatgcaaatc
    11581 cactccagaa ctcaacaggt ttgttctgcg gcgggtgtaa gtgtgaacac cggcatgtaa
    11641 gtgcgcttga gcaggagaat aaagcgctca gagacaccgg aggaatcctt tattttgcgt
    11701 tgcgcctcag gggattcgct gagccttctc ttcatggccc tgctcttcag acacaggtgc
    11761 tccgctgttt gtgtttt
//
