LOCUS       pZac2.1_gfaABC1D_H      6333 bp ds-DNA     circular SYN 17-JAN-2019
DEFINITION  pZac2.1 gfaABC1D-Lck-GCaMP3.
ACCESSION   urn.local...1427301926606.10
VERSION     .
KEYWORDS    pZac2.1_gfaABC1D_BioID2-BioID2-HA-WPRE
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Bal Khakh
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 29, 2020 from SnapGene 4.3.6
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        143..172
                     /label=lac_promoter
                     /note="/vntifkey=30"
     misc_feature    186..208
                     /label=M13_pUC_rev_primer
                     /note="/vntifkey=21"
     promoter        207..225
                     /label=M13_reverse_primer
                     /note="/vntifkey=30"
     promoter        449..1129
                     /label="gfaABC1D astrocyte minimal promoter"
                     /label="gfaABC1D   astrocyte   minimal promoter"
                     /note="/created_by=User"
                     /note="/vntifkey=30"
     misc_feature    1239..1371
                     /label=chim_int
                     /note="/vntifkey=21"
     primer_bind     1376..1397
                     /label="Chris primer F 194"
     promoter        1416..1434
                     /label=T7_promoter
                     /note="/vntifkey=30"
     primer          1425..1479
                     /label=pZac2.1-Igk-F
     CDS             1461..1523
                     /label=Igk
     CDS             1524..1550
                     /label=HA
     CDS             1551..2501
                     /label=HRP
     primer          complement(1556..1574)
                     /label=HRP-5-Seq-R
     primer          complement(2108..2126)
                     /label=HRP-seqR
     primer          2437..2456
                     /label=HRP-SeqF
     CDS             2502..2531
                     /label=Myc
     CDS             2535..2684
                     /label="PDGFR-beta-TM domain"
     primer          complement(2664..2705)
                     /label=pZac2.1-TM-R
     misc_feature    2706..3294
                     /label=WPRE
                     /note="/vntifkey=21"
     primer          complement(2781..2798)
                     /label=pWPRE-R
     terminator      3355..3474
                     /label=SV40_PA_terminator
                     /note="/vntifkey=43"
     misc_feature    3369..3388
                     /label=EBV_rev_primer
                     /note="/vntifkey=21"
     misc_feature    3786..3946
                     /label=lacZ_a
                     /note="/vntifkey=21"
     promoter        complement(3789..3805)
                     /label=M13_forward20_primer
                     /note="/vntifkey=30"
     misc_feature    3798..3820
                     /label=M13_pUC_fwd_primer
                     /note="/vntifkey=21"
     rep_origin      3964..4270
                     /direction=RIGHT
                     /label=f1_origin
                     /note="/vntifkey=33"
     promoter        4463..4491
                     /label=AmpR_promoter
                     /note="/vntifkey=30"
     gene            4533..5393
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/vntifkey=60"
     CDS             4533..5393
                     /codon_start=1
                     /label="ORF frame 1"
                     /label="ORF frame 1"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5548..6167
                     /direction=RIGHT
                     /label=pBR322_origin
                     /note="/vntifkey=33"
ORIGIN
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
       61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
      121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
      181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAGATTTA
      241 ATTAAGGCCT TAATTAGGCT GCGCGCTCGC TCGCTCACTG AGGCCGCCCG GGCAAAGCCC
      301 GGGCGTCGGG CGACCTTTGG TCGCCCGGCC TCAGTGAGCG AGCGAGCGCG CAGAGAGGGA
      361 GTGGCCAACT CCATCACTAG GGGTTCCTTG TAGTTAATGA TTAACCCGCC ATGCTACTTA
      421 TCTACGTAGC CATGCTCTAG GAAGATCTAA CATATCCTGG TGTGGAGTAG GGGACGCTGC
      481 TCTGACAGAG GCTCGGGGGC CTGAGCTGGC TCTGTGAGCT GGGGAGGAGG CAGACAGCCA
      541 GGCCTTGTCT GCAAGCAGAC CTGGCAGCAT TGGGCTGGCC GCCCCCCAGG GCCTCCTCTT
      601 CATGCCCAGT GAATGACTCA CCTTGGCACA GACACAATGT TCGGGGTGGG CACAGTGCCT
      661 GCTTCCCGCC GCACCCCAGC CCCCCTCAAA TGCCTTCCGA GAAGCCCATT GAGCAGGGGG
      721 CTTGCATTGC ACCCCAGCCT GACAGCCTGG CATCTTGGGA TAAAAGCAGC ACAGCCCCCT
      781 AGGGGCTGCC CTTGCTGTGT GGCGCCACCG GCGGTGGAGA ACAAGGCTCT ATTCAGCCTG
      841 TGCCCAGGAA AGGGGATCAG GGGATGCCCA GGCATGGACA GTGGGTGGCA GGGGGGGAGA
      901 GGAGGGCTGT CTGCTTCCCA GAAGTCCAAG GACACAAATG GGTGAGGGGA GAGCTCTCCC
      961 CATAGCTGGG CTGCGGCCCA ACCCCACCCC CTCAGGCTAT GCCAGGGGGT GTTGCCAGGG
     1021 GCACCCGGGC ATCGCCAGTC TAGCCCACTC CTTCATAAAG CCCTCGCATC CCAGGAGCGA
     1081 GCAGAGCCAG AGCAGGTTGG AGAGGAGACG CATCACCTCC GCTGCTCGCA AGCTTTATTG
     1141 CGGTAGTTTA TCACAGTTAA ATTGCTAACG CAGTCAGTGC TTCTGACACA ACAGTCTCGA
     1201 ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC
     1261 AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT
     1321 GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT
     1381 CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC
     1441 TCGAGCTCGG ATCCACCACC ATGGAGACAG ACACACTCCT GCTATGGGTA CTGCTGCTCT
     1501 GGGTTCCAGG TTCCACTGGT GACTATCCAT ATGATGTTCC AGATTATGCT GGGGCCCAGC
     1561 AGTTAACCCC TACATTCTAC GACAATAGCT GTCCCAACGT GTCCAACATC GTTCGCGACA
     1621 CAATCGTCAA CGAGCTCAGA TCCGATCCCA GGATCGCTGC TTCAATATTA CGTCTGCACT
     1681 TCCATGACTG CTTCGTGAAT GGTTGCGACG CTAGCATATT ACTGGACAAC ACCACCAGTT
     1741 TCCGCACTGA AAAGGATGCA TTCGGGAACG CTAACAGCGC CAGGGGCTTT CCAGTGATCG
     1801 ATCGCATGAA GGCTGCCGTT GAGTCAGCAT GCCCACGAAC AGTCAGTTGT GCAGACCTGC
     1861 TGACTATAGC TGCGCAACAG AGCGTGACTC TTGCAGGCGG ACCGTCCTGG AGAGTGCCGC
     1921 TCGGTCGACG TGACTCCCTA CAGGCATTCC TAGATCTGGC CAACGCCAAC TTGCCTGCTC
     1981 CATTCTTCAC CCTGCCCCAG CTGAAGGATA GCTTTAGAAA CGTGGGTCTG AATCGCTCGA
     2041 GTGACCTTGT GGCTCTGTCC GGAGGACACA CATTTGGAAA GAACCAGTGT AGGTTCATCA
     2101 TGGATAGGCT CTACAATTTC AGCAACACTG GGTTACCTGA CCCCACGCTG AACACTACGT
     2161 ATCTCCAGAC ACTGAGAGGC TTGTGCCCAC TGAATGGCAA CCTCAGTGCA CTAGTGGACT
     2221 TTGATCTGCG GACCCCAACC ATCTTCGATA ACAAGTACTA TGTGAATCTA GAGGAGCAGA
     2281 AAGGCCTGAT ACAGAGTGAT CAAGAACTGT TTAGCAGTCC AAACGCCACT GACACCATCC
     2341 CACTGGTGAG AAGTTTTGCT AACTCTACTC AAACCTTCTT TAACGCCTTC GTGGAAGCCA
     2401 TGGACCGTAT GGGTAACATT ACCCCTCTGA CGGGTACCCA AGGCCAGATT CGTCTGAACT
     2461 GCAGAGTGGT CAACAGCAAC TCTCCGCGGC TGCAGGTCGA CGAACAAAAA CTCATCTCAG
     2521 AAGAGGATCT GAATGCTGTG GGCCAGGACA CGCAGGAGGT CATCGTGGTG CCACACTCCT
     2581 TGCCCTTTAA GGTGGTGGTG ATCTCAGCCA TCCTGGCCCT GGTGGTGCTC ACCATCATCT
     2641 CCCTTATCAT CCTCATCATG CTTTGGCAGA AGAAGCCACG TTAGGGATCC AATTCGCTTA
     2701 TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG
     2761 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT
     2821 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG
     2881 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC
     2941 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC
     3001 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC
     3061 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC
     3121 TGCTCGCCTA TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG
     3181 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC
     3241 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT
     3301 ACCGGAATTC TGCAGATATC TAGAGTCGAC CCGGGCGGCC GCTTCGAGCA GACATGATAA
     3361 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
     3421 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
     3481 ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGATGTGG GAGGTTTTTT
     3541 AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCTTCCT AGAGCATGGC
     3601 TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG
     3661 TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC
     3721 CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA
     3781 CCTAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA
     3841 CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC
     3901 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGACGC GCCCTGTAGC
     3961 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC
     4021 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT
     4081 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC
     4141 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG
     4201 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA
     4261 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG
     4321 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC
     4381 AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA
     4441 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
     4501 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
     4561 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
     4621 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
     4681 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
     4741 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
     4801 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
     4861 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
     4921 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
     4981 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
     5041 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
     5101 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
     5161 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
     5221 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
     5281 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
     5341 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
     5401 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
     5461 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
     5521 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     5581 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     5641 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     5701 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     5761 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     5821 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     5881 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     5941 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     6001 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
     6061 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     6121 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     6181 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
     6241 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
     6301 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAG
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