LOCUS       pAAV-CAG-HRP-TM            5954 bp ds-DNA     circular SYN 21-FEB-2024
DEFINITION  AAV-mediated expression of tdTomato (codon diversified) under the 
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-HRP-TM
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 6161)
  TITLE     Accessory plasmids for optogenetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2024 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label="AAV2 ITR"
     primer_bind     1002..1024
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1086..1105
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer          1118..1167
                     /label=CAG-HRP-F
     unsure          1144..1149
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1150..2373
                     /label=igk-HA-HRP-TM
     primer          complement(2354..2404)
                     /label=CAG-HRP-R
     misc_feature    2398..2986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2451..2471)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2869..2880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label="Factor Xa site"
                     /translation="IEGR"
     polyA_signal    3029..3150
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3040..3059)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3094..3113
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3312..3441
                     /label="AAV2 ITR"
     primer_bind     complement(3507..3524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3678..4266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3758..3777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4437..5297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5060..5079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5298..5402)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      complement(5428..5883)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5565..5586)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5777..5796
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACGTA GCCATGCTCT
      181 AGGAAGAGTA CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
      241 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
      301 AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
      361 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
      421 AGTCATCGCT ATTACCATGG TCGAGGTGAG CCCCACGTTC TGCTTCACTC TCCCCATCTC
      481 CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT TTAATTATTT TGTGCAGCGA
      541 TGGGGGCGGG GGGGGGGGGG GGGGGGGCGC GCGCCAGGCG GGGCGGGGCG GGGCGAGGGG
      601 CGGGGCGGGG CGAGGCGGAG AGGTGCGGCG GCAGCCAATC AGAGCGGCGC GCTCCGAAAG
      661 TTTCCTTTTA TGGCGAGGCG GCGGCGGCGG CGGCCCTATA AAAAGCGAAG CGCGCGGCGG
      721 GCGGGAGTCG CTGCGCGCTG CCTTCGCCCC GTGCCCCGCT CCGCCGCCGC CTCGCGCCGC
      781 CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA GCGGGCGGGA CGGCCCTTCT
      841 CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTTG TTTCTTTTCT GTGGCTGCGT
      901 GAAAGCCTTG AGGGGCTCCG GGAGGGCCCT TTGTGCGGGG GGAGCGGCTC GGGGCTGTCC
      961 GCGGGGGGAC GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT
     1021 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
     1081 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGGATCCGGT
     1141 ACCGCCACCA TGGAGACAGA CACACTCCTG CTATGGGTAC TGCTGCTCTG GGTTCCAGGT
     1201 TCCACTGGTG ACTATCCATA TGATGTTCCA GATTATGCTG GGGCCCAGCA GTTAACCCCT
     1261 ACATTCTACG ACAATAGCTG TCCCAACGTG TCCAACATCG TTCGCGACAC AATCGTCAAC
     1321 GAGCTCAGAT CCGATCCCAG GATCGCTGCT TCAATATTAC GTCTGCACTT CCATGACTGC
     1381 TTCGTGAATG GTTGCGACGC TAGCATATTA CTGGACAACA CCACCAGTTT CCGCACTGAA
     1441 AAGGATGCAT TCGGGAACGC TAACAGCGCC AGGGGCTTTC CAGTGATCGA TCGCATGAAG
     1501 GCTGCCGTTG AGTCAGCATG CCCACGAACA GTCAGTTGTG CAGACCTGCT GACTATAGCT
     1561 GCGCAACAGA GCGTGACTCT TGCAGGCGGA CCGTCCTGGA GAGTGCCGCT CGGTCGACGT
     1621 GACTCCCTAC AGGCATTCCT AGATCTGGCC AACGCCAACT TGCCTGCTCC ATTCTTCACC
     1681 CTGCCCCAGC TGAAGGATAG CTTTAGAAAC GTGGGTCTGA ATCGCTCGAG TGACCTTGTG
     1741 GCTCTGTCCG GAGGACACAC ATTTGGAAAG AACCAGTGTA GGTTCATCAT GGATAGGCTC
     1801 TACAATTTCA GCAACACTGG GTTACCTGAC CCCACGCTGA ACACTACGTA TCTCCAGACA
     1861 CTGAGAGGCT TGTGCCCACT GAATGGCAAC CTCAGTGCAC TAGTGGACTT TGATCTGCGG
     1921 ACCCCAACCA TCTTCGATAA CAAGTACTAT GTGAATCTAG AGGAGCAGAA AGGCCTGATA
     1981 CAGAGTGATC AAGAACTGTT TAGCAGTCCA AACGCCACTG ACACCATCCC ACTGGTGAGA
     2041 AGTTTTGCTA ACTCTACTCA AACCTTCTTT AACGCCTTCG TGGAAGCCAT GGACCGTATG
     2101 GGTAACATTA CCCCTCTGAC GGGTACCCAA GGCCAGATTC GTCTGAACTG CAGAGTGGTC
     2161 AACAGCAACT CTCCGCGGCT GCAGGTCGAC GAACAAAAAC TCATCTCAGA AGAGGATCTG
     2221 AATGCTGTGG GCCAGGACAC GCAGGAGGTC ATCGTGGTGC CACACTCCTT GCCCTTTAAG
     2281 GTGGTGGTGA TCTCAGCCAT CCTGGCCCTG GTGGTGCTCA CCATCATCTC CCTTATCATC
     2341 CTCATCATGC TTTGGCAGAA GAAGCCACGT TAGGAATTCG ATATCAAGCT TATCGATAAT
     2401 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT
     2461 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG
     2521 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG
     2581 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT
     2641 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT
     2701 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG
     2761 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC
     2821 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT
     2881 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC
     2941 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA
     3001 CCCGGGCGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA
     3061 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT
     3121 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT
     3181 CAGGTTCAGG GGGAGATGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
     3241 AAAATCGATA AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT
     3301 CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC
     3361 GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC
     3421 AGTGAGCGAG CGAGCGCGCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
     3481 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
     3541 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
     3601 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     3661 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     3721 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     3781 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     3841 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     3901 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     3961 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     4021 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     4081 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
     4141 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     4201 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     4261 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     4321 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     4381 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     4441 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     4501 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     4561 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     4621 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     4681 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     4741 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     4801 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     4861 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     4921 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     4981 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     5041 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     5101 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     5161 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     5221 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     5281 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     5341 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     5401 CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG
     5461 CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC
     5521 CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA
     5581 GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG
     5641 ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG
     5701 CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA
     5761 ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG
     5821 TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG
     5881 CGTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT
     5941 CGCTATTACG CCAG
//
