LOCUS       71_CMV_Zdk3-VPR_       16927 bp    DNA     circular UNA 28-NOV-2024
DEFINITION  Generated by Golden Gate/Type IIS Cloning (3347-P2A-bb,
            3579_Zdk3-VPR PCR Product).
ACCESSION   urn.local...2a-fwjsr43
VERSION     urn.local...2a-fwjsr43
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        2672..3850
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /standard_name="EF-1-alpha promoter"
     intron          2903..3841
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
                     /standard_name="EF-1-alpha intron A"
     CDS             3875..3895
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /codon_start=1
                     /translation="PKKKRKV"
                     /transl_table=1
                     /standard_name="SV40 NLS"
     CDS             3929..4102
                     /translation="VLVARQEIYWLPNLNWEQKFAFISSLTNDPSQSANLLAEAKKLN
                     GAQP"
                     /transl_table=1
                     /standard_name="Zdk3"
     CDS             4157..4306
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDML"
                     /codon_start=1
                     /transl_table=1
                     /product="tetrameric repeat of the minimal activation
                     domain of herpes simplex virus VP16 (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/9843940""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/9843940"">Beerl
                     i et al., 1998</a>)"
                     /standard_name="VP64"
     CDS             4775..5131
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDN
                     SEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLS
                     GDEDFSSIADMDFSALL"
                     /codon_start=1
                     /transl_table=1
                     /product="transcriptional activation domain of human
                     RelA,also known as p65
                     (<ahref=""http://www.ncbi.nlm.nih.gov/pubmed/18631125""tit
                     le=""http://www.ncbi.nlm.nih.gov/pubmed/18631125"">O'Shea
                     and Perkins, 2008</a>)"
                     /standard_name="RelA (p65) AD"
     CDS             5150..5719
                     /codon_start=1
                     /transl_table=1
                     /product="transcriptional activation domain from the
                     humanherpesvirus 4 (Epstein-Barr virus) replication
                     andtranscription activator Rta/BRLF1
                     (ahref=""http://www.ncbi.nlm.nih.gov/pubmed/1323708""title
                     =""http://www.ncbi.nlm.nih.gov/pubmed/1323708""Hardwick et
                     al.,
                     1992/a;ahref=""http://www.ncbi.nlm.nih.gov/pubmed/25730490
                     ""title=""http://www.ncbi.nlm.nih.gov/pubmed/25730490""Cha
                     vez et al., 2015/a)"
                     /standard_name="Rta AD"
     CDS             5741..5761
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /codon_start=1
                     /translation="PKKKRKV"
                     /transl_table=1
                     /standard_name="SV40 NLS"
     CDS             5840..5860
                     /codon_start=1
                     /locus_tag="<br>"
                     /note="<br>"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /protein_id="<br>"
                     /transl_table=1
                     /translation="PKKKRKV"
                     /standard_name="SV40 NLS"
     CDS             5870..5896
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc
                     proto-oncogenea
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/3054508""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/3054508""(Dang
                     and Lee, 1988)/a"
                     /transl_table=1
                     /translation="PAAKRVKLD"
                     /standard_name="c-myc NLS"
     CDS             5906..5926
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /codon_start=1
                     /transl_table=1
                     /translation="PKKKRKV"
                     /standard_name="SV40 NLS"
     CDS             5936..10036
                     /product="catalytically dead mutant of the Cas9
                     endonuclease from the Streptococcus pyogenes Type II
                     CRISPR/Cas system"
                     /note="RNA-guided DNA-binding protein that lacks
                     endonuclease activity due to the D10A mutation in the RuvC
                     catalytic domain and the H840A mutation in the HNH
                     catalytic domain"
                     /codon_start=1
                     /transl_table=1
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
                     KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV
                     KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH
                     PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVL
                     TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
                     AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /standard_name="dCas9"
     CDS             10064..10488
                     /codon_start=1
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILG
                     RNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGD
                     VQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAK"
                     /transl_table=1
                     /standard_name="AsLOV2 hs"
     CDS             10517..10945
                     /codon_start=1
                     /transl_table=1
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILG
                     RNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGD
                     VQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
                     /standard_name="AsLOV2 hs"
     CDS             10973..11397
                     /codon_start=1
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILG
                     RNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGD
                     VQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAK"
                     /transl_table=1
                     /standard_name="AsLOV2 hs"
     misc_feature    11445..12033
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /standard_name="WPRE"
     LTR             12558..12738
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /standard_name="5' LTR (truncated)"
     polyA_signal    12770..12994
                     /note="bovine growth hormone polyadenylation signal"
                     /standard_name="bGH poly(A) signal"
     rep_origin      13204..13632
     promoter        13646..13975
                     /note="SV40 enhancer and early promoter"
                     /standard_name="SV40 promoter"
     promoter        14023..14070
                     /note="synthetic bacterial promoter"
                     /standard_name="EM7 promoter"
     CDS             14089..14463
                     /product="antibiotic-binding protein"
                     /note="confers resistance to bleomycin, phleomycin, and
                     Zeocin(TM)"
                     /codon_start=1
                     /transl_table=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /standard_name="BleoR"
     polyA_signal    14593..14714
                     /note="SV40 polyadenylation signal"
                     /standard_name="SV40 poly(A) signal"
     protein_bind    14787..14803
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator"
     promoter        complement(14811..14841)
                     /note="promoter for the E. coli lac operon"
                     /standard_name="lac promoter"
     protein_bind    14856..14877
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /standard_name="CAP binding site"
     rep_origin      complement(15165..15753)
     CDS             complement(15924..16784)
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /standard_name="AmpR"
     promoter        complement(16785..16889)
                     /gene="bla"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
       61 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      121 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      181 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      241 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      301 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      361 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      421 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      481 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      541 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      601 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      661 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      721 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      781 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      841 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      901 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      961 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1021 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1081 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1141 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1201 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1261 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1321 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1381 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1441 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1501 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1561 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1621 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1681 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1741 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1801 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1861 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1921 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1981 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     2041 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     2101 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     2161 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2221 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2281 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2341 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2401 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2461 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2521 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2581 tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
     2641 gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     2701 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     2761 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     2821 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     2881 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     2941 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     3001 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     3061 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     3121 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     3181 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     3241 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     3301 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     3361 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     3421 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     3481 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     3541 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     3601 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
     3661 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     3721 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     3781 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     3841 ggtgtcgtga cgtacggcca ccatgggtag cggacctaag aaaaagagga aggtggcggc
     3901 cgctggaggt tctggaagtg gatccatggt ggataacaaa ttcaataaag aagtgctggt
     3961 ggcccggcag gaaatctatt ggctgccaaa cctgaattgg gagcagaagt ttgccttcat
     4021 cagtagcctg actaatgatc catctcagag cgccaatctg ctggccgaag ccaaaaaact
     4081 gaacggtgcc cagcccccaa aaggaggagg tggaagcgga ggcggcggaa gtgcggccgc
     4141 tggatccgga cgggctgacg cattggacga ttttgatctg gatatgctgg gaagtgacgc
     4201 cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact ttgacctcga
     4261 catgctcggc agtgacgccc ttgatgattt cgacctggac atgctgatta acactagtgg
     4321 cggctctggc ggcggctccg gaggctctag ccagtacctg cccgacaccg acgacaggca
     4381 ccggatcgag gaaaagcgga agcggaccta cgagacattc aagagcatca tgaagaagtc
     4441 ccccttcagc ggccccaccg accctagacc tccacctaga agaatcgccg tgcccagcag
     4501 atccagcgcc agcgtgccaa aacctgcccc ccagccttac cccttcacca gcagcctgag
     4561 caccatcaac tacgacgagt tccctaccat ggtgttcccc agcggccaga tctctcaggc
     4621 ctctgctctg gctccagccc ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc
     4681 tccagccatg gtgtctgcac tggctcaggc accagcaccc gtgcctgtgc tggctcctgg
     4741 acctccacag gctgtggctc caccagcccc taaacctaca caggccggcg agggcacact
     4801 gtctgaagct ctgctgcagc tgcagttcga cgacgaggat ctgggagccc tgctgggaaa
     4861 cagcaccgat cctgccgtgt tcaccgacct ggccagcgtg gacaacagcg agttccagca
     4921 gctgctgaac cagggcatcc ctgtggcccc tcacaccacc gagcccatgc tgatggaata
     4981 ccccgaggcc atcacccggc tcgtgacagg cgctcagagg cctcctgatc cagctcctgc
     5041 ccctctggga gcaccaggcc tgcctaatgg actgctgtct ggcgacgagg acttcagctc
     5101 tatcgccgat atggatttct cagccttgct gggctctggc agcggcagcc gggattccag
     5161 ggaagggatg tttttgccga agcctgaggc cggctccgct attagtgacg tgtttgaggg
     5221 ccgcgaggtg tgccagccaa aacgaatccg gccatttcat cctccaggaa gtccatgggc
     5281 caaccgccca ctccccgcca gcctcgcacc aacaccaacc ggtccagtac atgagccagt
     5341 cgggtcactg accccggcac cagtccctca gccactggac ccagcgcccg cagtgactcc
     5401 cgaggccagt cacctgttgg aggaccccga tgaagagacg agccaggctg tcaaagccct
     5461 tcgggagatg gccgatactg tgattcccca gaaggaagag gctgcaatct gtggccaaat
     5521 ggacctttcc catccgcccc caaggggcca tctggatgag ctgacaacca cacttgagtc
     5581 catgaccgag gatctgaacc tggactcacc cctgaccccg gaattgaacg agattctgga
     5641 taccttcctg aacgacgagt gcctcttgca tgccatgcat atcagcacag gactgtccat
     5701 cttcgacaca tctctgtttg gttctggaag tggatccagc cccaagaaga agagaaaggt
     5761 ggaggccagc ggcagtggag ctacaaactt tagccttctc aaacaagcag gagatgtcga
     5821 agagaatcct ggcccgagcc ccaagaagaa gaggaaagtc ggctctggcc ctgccgctaa
     5881 gagagtgaag ctggacggaa gcggacccaa gaagaagaga aaggtggagg ccagcgacaa
     5941 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
     6001 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     6061 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     6121 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     6181 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     6241 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     6301 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     6361 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     6421 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     6481 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     6541 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     6601 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
     6661 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     6721 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     6781 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     6841 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     6901 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     6961 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     7021 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     7081 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     7141 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     7201 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     7261 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     7321 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     7381 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     7441 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     7501 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     7561 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     7621 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     7681 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     7741 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     7801 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     7861 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     7921 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     7981 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     8041 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     8101 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     8161 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     8221 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     8281 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     8341 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     8401 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     8461 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     8521 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     8581 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     8641 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     8701 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     8761 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     8821 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     8881 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     8941 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     9001 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     9061 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     9121 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     9181 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     9241 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     9301 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     9361 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     9421 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     9481 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     9541 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     9601 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     9661 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     9721 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     9781 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     9841 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     9901 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     9961 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
    10021 tcagctggga ggcgacggtt ctggaagtgg atccggaagc ggacttgcta caactttgga
    10081 gcggattgag aagaatttcg tcataactga tcccaggttg cctgataatc ctataatatt
    10141 cgcctccgat agttttcttc aacttacgga gtactccaga gaggagatac tgggaaggaa
    10201 ttgcagattc ctccaaggtc cagaaactga tagagctact gtgaggaaga tacgggatgc
    10261 cattgacaat caaactgaag tgacagtgca acttatcaat tacaccaagt cagggaaaaa
    10321 attctggaac ctgttccatt tgcaacccat gagggaccaa aagggcgatg tgcaatattt
    10381 cataggcgtt caacttgatg gtactgaaca cgtcagagat gcggcggagc gagaaggagt
    10441 gatgctgatt aagaaaacag cagagaatat cgacgaagcg gcgaaagagt tgagcgctgg
    10501 aggaggtgga agcggacttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac
    10561 tgatcccagg ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac
    10621 ggagtactcc agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac
    10681 tgatagagct actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt
    10741 gcaacttatc aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc
    10801 catgagggac caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga
    10861 acacgtcaga gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa
    10921 tatcgacgaa gcggcgaaag agttgggttc tggaagtgga tccggaagcg gacttgctac
    10981 aactttggag cggattgaga agaatttcgt cataactgat cccaggttgc ctgataatcc
    11041 tataatattc gcctccgata gttttcttca acttacggag tactccagag aggagatact
    11101 gggaaggaat tgcagattcc tccaaggtcc agaaactgat agagctactg tgaggaagat
    11161 acgggatgcc attgacaatc aaactgaagt gacagtgcaa cttatcaatt acaccaagtc
    11221 agggaaaaaa ttctggaacc tgttccattt gcaacccatg agggaccaaa agggcgatgt
    11281 gcaatatttc ataggcgttc aacttgatgg tactgaacac gtcagagatg cggcggagcg
    11341 agaaggagtg atgctgatta agaaaacagc agagaatatc gacgaagcgg cgaaagagtt
    11401 gtaaagcgaa gtcaatccca gaattcgata tcaagcttat cgataatcaa cctctggatt
    11461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
    11521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
    11581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
    11641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
    11701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
    11761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    11821 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
    11881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    11941 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
    12001 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
    12061 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
    12121 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
    12181 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
    12241 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
    12301 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
    12361 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
    12421 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
    12481 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
    12541 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    12601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    12661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    12721 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
    12781 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    12841 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    12901 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    12961 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    13021 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggggggggg
    13081 ggggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
    13141 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
    13201 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    13261 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    13321 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    13381 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    13441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    13501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    13561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    13621 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    13681 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    13741 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    13801 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    13861 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
    13921 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    13981 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
    14041 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
    14101 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
    14161 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
    14221 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
    14281 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
    14341 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
    14401 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    14461 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    14521 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    14581 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    14641 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    14701 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    14761 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    14821 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    14881 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    14941 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    15001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    15061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    15121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    15181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    15241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    15301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    15361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    15421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    15481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    15541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    15601 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    15661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    15721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    15781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    15841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    15901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    15961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    16021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    16081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    16141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    16201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    16261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    16321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    16381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    16441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    16501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    16561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    16621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    16681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    16741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    16801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    16861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    16921 gacggat
//
