LOCUS       51_PAC_E83_LOV_e        7253 bp ds-DNA     circular     25-NOV-2024
DEFINITION  .
ACCESSION   urn.local...11-ihq0464
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             5..370
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="Puromycin N-acetyltransferase"
     CDS             380..433
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             join(434..436,437..439,440..1150)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="mammalian codon-optimized"
                     /product="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     polyA_signal    1231..1455
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      1501..1929
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /direction="RIGHT"
                     /label="f1 ori"
     promoter        1943..2272
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             2339..3133
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin(R))"
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    3307..3428
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3879..4464)
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /direction="LEFT"
                     /label="ori"
     CDS             complement(4635..5495)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to ampicillin, carbenicillin,
                     andrelated antibiotics"
                     /gene="bla"
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR"
     promoter        complement(5496..5600)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /gene="bla"
                     /label="AmpR promoter"
     enhancer        5866..6245
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        6246..6347
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        6348..6449
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        6494..6512
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             6568..6816
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="Puromycin N-acetyltransferase"
     CDS             6823..7251
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 transl_table: 1 translation:
                     LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRA
                     TVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEH
                     VRDAAEREGVMLIKKTAENIDEAAKEL"
                     /label="AsLOV2 hs"
ORIGIN      
        1 gagcgccggt gctgtgttcg ccgagatcgg cccgcgcatg gctgaactct ccggaagtag
       61 acttgcagcc cagcaacaga tggaggggct tctcgcacca caccgcccca aagaaccagc
      121 ttggttcctt gctactgtgg gcgtgtcccc ggatcaccag gggaagggac ttggaagtgc
      181 agtagtgctt cctggggtcg aggctgcgga acgcgctggt gtcccagctt ttttggaaac
      241 ttccgccccg cgcaacctgc cattctatga gagactcggc tttactgtta ctgcagatgt
      301 cgaagtacct gagggaccta ggacttggtg tatgacccgc aagcctgggg ctggctccac
      361 agggagcaga ggaagcggag agggcagggg aagtcttcta acatgcgggg acgtggagga
      421 aaatcccggc cccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      481 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      541 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      601 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      661 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      721 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      781 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      841 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      901 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      961 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1021 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1081 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1141 gctgtacaag tgagcttgaa ttccaccaca ctggactagt ggatccgagc tcggtaccaa
     1201 gcttaagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     1261 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     1321 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     1381 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     1441 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     1501 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1561 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1621 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1681 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1741 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1801 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1861 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1921 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     1981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     2041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     2101 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     2161 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     2221 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     2281 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     2341 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     2401 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     2461 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     2521 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     2581 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     2641 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     2701 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     2761 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     2821 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     2881 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     2941 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     3001 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     3061 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     3121 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     3181 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     3241 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     3301 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3421 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     3481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4381 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt
     4441 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4501 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4561 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4621 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4681 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4741 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4801 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4861 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4921 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4981 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5041 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5101 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5161 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5221 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5281 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5341 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5401 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5461 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5521 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5581 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     5641 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5701 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     5761 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     5821 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     5881 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5941 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     6001 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     6061 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     6121 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     6181 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     6241 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     6301 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     6361 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     6421 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     6481 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaacg
     6541 ggccctctag actcgaggga ggccaccatg actgagtata aaccaacagt taggttggca
     6601 acaagggacg atgtcccgcg agcggttaga acacttgctg ccgcttttgc ggattatccc
     6661 gcgacccggc acaccgtaga cccggacaga catatagaac gagtcacgga acttcaagag
     6721 ttgtttctta caagagtagg gctcgacatc gggaaggtct gggttgctga tgatggagct
     6781 gcagtagctg tttggacaac ccctgaatca gtggaatctg gtcttgctac aactttggag
     6841 cggattgaga agaatttcgt cataactgat cccaggttgc ctgataatcc tataatattc
     6901 gcctccgata gttttcttca acttacggag tactccagag aggagatact gggaaggaat
     6961 tgcagattcc tccaaggtcc agaaactgat agagctactg tgaggaagat acgggatgcc
     7021 attgacaatc aaactgaagt gacagtgcaa cttatcaatt acaccaagtc agggaaaaaa
     7081 ttctggaacc tgttccattt gcaacccatg agggaccaaa agggcgatgt gcaatatttc
     7141 ataggcgttc aacttgatgg tactgaacac gtcagagatg cggcggagcg agaaggagtg
     7201 atgctgatta agaaaacagc agagaatatc gacgaagcgg cgaaagagtt ggg
//
