LOCUS       #231614_pS5             6960 bp ds-DNA     circular     19-MAR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     Linker          303..349
                     /label="LMS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Terminator      360..462
                     /label="SEVA_T0"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     Selection Ma... 489..1415
                     /label="SEVA_Km"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Ori_Transfer    1437..1682
                     /label="SEVA_oriT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1691..3211
                     /label="SEVA_pBBR1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Terminator      3226..3330
                     /label="SEVA_T1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     Linker          3346..3392
                     /label="LMP"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     regulatory      3397..3457
                     /label="Terminator1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3458..3477
                     /label="SpacerA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Promoter        3478..3766
                     /label="pBAD"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     Promoter        complement(3575..3656)
                     /label="paraC Promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     Promoter        3690..3766
                     /label="araBAD Promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     misc_feature    3767..3841
                     /label="RiboA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Linker          3848..3879
                     /label="UTR1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     Ribosomal Bi... 3881..3894
                     /label="RBS2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     Repressor       3899..4522
                     /label="TetR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     Linker          4529..4560
                     /label="UTR3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Ribosomal Bi... 4562..4575
                     /label="RBS3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Reporter        4580..5290
                     /label="superfolder GFP"
                     /ApEinfo_revcolor="#a2d39c"
                     /ApEinfo_fwdcolor="#a2d39c"
     Linker          5297..5341
                     /label="L1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      5346..5396
                     /label="Terminator3"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    5397..5416
                     /label="SpacerA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Promoter        5417..5492
                     /label="pTet"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    5493..5567
                     /label="RiboC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Linker          5574..5605
                     /label="UTR2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Ribosomal Bi... 5607..5620
                     /label="RBS3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Repressor       5625..6503
                     /label="AraC"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     Linker          6510..6541
                     /label="UTR3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Ribosomal Bi... 6543..6556
                     /label="RBS3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Reporter        6561..296
                     /label="mKate2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 AAAAAAAAAC TCTTGGTTGG GAAGCATCAA CAGAAACCTT ATATCCTGCG GACGGTGGCT
       61 TAGAAGGACG CGCAGACATG GCACTGAAAT TAGTTGGAGG CGGTCATTTA ATCTGCAACC
      121 TGAAAACAAC CTATCGTTCC AAAAAACCCG CTAAAAACCT TAAAATGCCT GGAGTATACT
      181 ATGTTGATCG TCGCTTAGAA CGTATTAAAG AAGCTGATAA AGAAACCTAC GTTGAACAAC
      241 ATGAAGTAGC CGTAGCCCGT TATTGTGACC TTCCGTCGAA ATTAGGACAT CGTTGAGGCT
      301 CGGGAGACCT ATCGGTAATA ACAGTCCAAT CTGGTGTAAC TTCGGAATCG TCCACTAGTC
      361 TTGGACTCCT GTTGATAGAT CCAGTAATGA CCTCAGAACT CCATCTGGAT TTGTTCAGAA
      421 CGCTCGGTTG CCGCCGGGCG TTTTTTATTG GTGAGAATCC AGGGGTCCCC AATAATTACG
      481 ATTTAAATTT GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC
      541 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT
      601 TCAGCGTGAA ACGAGCTGTA GCCGTCCGCG TCTGAACAGC AACATGGATG CGGATCTGTA
      661 TGGCTATAAA TGGGCGCGTG ATAACGTGGG TCAGAGCGGC GCGACCATTT ATCGTCTGTA
      721 TGGCAAACCG GATGCGCCGG AACTGTTTCT GAAACATGGC AAAGGCAGCG TGGCGAACGA
      781 TGTGACCGAT GAAATGGTGC GTCTGAACTG GCTGACCGAA TTTATGCCGC TGCCGACCAT
      841 TAAACATTTT ATTCGCACCC CGGATGATGC GTGGCTGCTG ACCACCGCGA TTCCGGGCAA
      901 AACCGCGTTT CAGGTGCTGG AAGAATATCC GGATAGCGGC GAAAACATTG TGGATGCGCT
      961 GGCCGTGTTT CTGCGTCGTC TGCATAGCAT TCCGGTGTGC AACTGCCCGT TTAACAGCGA
     1021 TCGTGTGTTT CGTCTGGCCC AGGCGCAGAG CCGTATGAAC AACGGCCTGG TGGATGCGAG
     1081 CGATTTTGAT GATGAACGTA ACGGCTGGCC GGTGGAACAG GTGTGGAAAG AAATGCATAA
     1141 ACTGCTGCCG TTTAGCCCGG ATAGCGTGGT GACCCACGGC GATTTTAGCC TGGATAACCT
     1201 GATTTTCGAT GAAGGCAAAC TGATTGGCTG CATTGATGTG GGCCGTGTGG GCATTGCGGA
     1261 TCGTTATCAG GATCTGGCCA TTCTGTGGAA CTGCCTGGGC GAATTTAGCC CGAGCCTGCA
     1321 AAAACGTCTG TTTCAGAAAT ATGGCATTGA TAATCCGGAT ATGAACAAAC TGCAATTTCA
     1381 TCTGATGCTG GATGAATTTT TCTAATAATT AATTGGACCG CGGTCCGCGC GTTGTCCTTT
     1441 TCCGCTGCAT AACCCTGCTT CGGGGTCATT ATAGCGATTT TTTCGGTATA TCCATCCTTT
     1501 TTCGCACGAT ATACAGGATT TTGCCAAAGG GTTCGTGTAG ACTTTCCTTG GTGTATCCAA
     1561 CGGCGTCAGC CGGGCAGGAT AGGTGAAGTA GGCCCACCCG CGAGCGGGTG TTCCTTCTTC
     1621 ACTGTCCCTT ATTCGCACCT GGCGGTGCTC AACGGGAATC CTGCTCTGCG AGGCTGGCCG
     1681 TAGGCCGGCC CTACCGGCGC GGCAGCGTTA CCCGTGTCGG CGGCTCCAAC GGCTCGCCAT
     1741 CGTCCAGAAA ACACGGCTCA TCGGGCATCG GCAGGCGCTG CTGCCCGCGC CGTTCCCATT
     1801 CCTCCGTTTC GGTCAAGGCT GGCAGGTCTG GTTCCATGCC CGGAATGCCG GGCTGGCTGG
     1861 GCGGCTCCTC GCCGGGGCCG GTCGGTAGTT GCTGCTCGCC CGGATACAGG GTCGGGATGC
     1921 GGCGCAGGTC GCCATGCCCC AACAGCGATT CGTCCTGGTC GTCGTGATCA ACCACCACGG
     1981 CGGCACTGAA CACCGACAGG CGCAACTGGT CGCGGGGCTG GCCCCACGCC ACGCGGTCAT
     2041 TGACCACGTA GGCCGACACG GTGCCGGGGC CGTTGAGCTT CACGACGGAG ATCCAGCGCT
     2101 CGGCCACCAA GTCCTTGACT GCGTATTGGA CCGTCCGCAA AGAACGTCCG ATGAGCTTGG
     2161 AAAGTGTCTT CTGGCTGACC ACCACGGCGT TCTGGTGGCC CATCTGCGCC ACGAGGTGAT
     2221 GCAGCAGCAT TGCCGCCGTG GGTTTCCTCG CAATAAGCCC GGCCCACGCC TCATGCGCTT
     2281 TGCGTTCCGT TTGCACCCAG TGACCGGGCT TGTTCTTGGC TTGAATGCCG ATTTCTCTGG
     2341 ACTGCGTGGC CATGCTTATC TCCATGCGGT AGGGGTGCCG CACGGTTGCG GCACCATGCG
     2401 CAATCAGCTG CAACTTTTCG GCAGCGCGAC AACAATTATG CGTTGCGTAA AAGTGGCAGT
     2461 CAATTACAGA TTTTCTTTAA CCTACGCAAT GAGCTATTGC GGGGGGTGCC GCAATGAGCT
     2521 GTTGCGTACC CCCCTTTTTT AAGTTGTTGA TTTTTAAGTC TTTCGCATTT CGCCCTATAT
     2581 CTAGTTCTTT GGTGCCCAAA GAAGGGCACC CCTGCGGGGT TCCCCCACGC CTTCGGCGCG
     2641 GCTCCCCCTC CGGCAAAAAG TGGCCCCTCC GGGGCTTGTT GATCGACTGC GCGGCCTTCG
     2701 GCCTTGCCCA AGGTGGCGCT GCCCCCTTGG AACCCCCGCA CTCGCCGCCG TGAGGCTCGG
     2761 GGGGCAGGCG GGCGGGCTTC GCCCTTCGAC TGCCCCCACT CGCATAGGCT TGGGTCGTTC
     2821 CAGGCGCGTC AAGGCCAAGC CGCTGCGCGG TCGCTGCGCG AGCCTTGACC CGCCTTCCAC
     2881 TTGGTGTCCA ACCGGCAAGC GAAGCGCGCA GGCCGCAGGC CGGAGGCTTT TCCCCAGAGA
     2941 AAATTAAAAA AATTGATGGG GCAAGGCCGC AGGCCGCGCA GTTGGAGCCG GTGGGTATGT
     3001 GGTCGAAGGC TGGGTAGCCG GTGGGCAATC CCTGTGGTCA AGCTCGTGGG CAGGCGCAGC
     3061 CTGTCCATCA GCTTGTCCAG CAGGGTTGTC CACGGGCCGA GCGAAGCGAG CCAGCCGGTG
     3121 GCCGCTCGCG GCCATCGTCC ACATATCCAC GGGCTGGCAA GGGAGCGCAG CGACCGCGCA
     3181 GGGCGAAGCC CGGAGAGCAA GCCCGTAGGG GGGCGCGCCC AGCTGTCTAG GGCGGCGGAT
     3241 TTGTCCTACT CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT
     3301 TCGACTGAGC CTTTCGTTTT ATTTGATGCC TTTAATTAAG GCTCGGGTAA GAACTCGCAC
     3361 TTCGTGGAAA CACTATTATC TGGTGGGTCT CTGTCCCTCG GTACCAAATT CCAGAAAAGA
     3421 GGCCTCCCGA AAGGGGGGCC TTTTTTCGTT TTGGTCCGTG CCTACTCTGG AAAATCTAGA
     3481 AACCAATTGT CCATATTGCA TCAGACATTG CCGTCACTGC GTCTTTTACT GGCTCTTCTC
     3541 GCTAACCAAA CCGGTAACCC CGCTTATTAA AAGCATTCTG TAACAAAGCG GGACCAAAGC
     3601 CATGACAAAA ACGCGTAACA AAAGTGTCTA TAATCACGGC AGAAAAGTCC ACATTGATTA
     3661 TTTGCACGGC GTCACACTTT GCTATGCCAT AGCATTTTTA TCCATAAGAT TAGCGGATCC
     3721 TACCTGACGC TTTTTATCGC AACTCTCTAC TGTTTCTCCA TACCCGAGCT GTCACCGGAT
     3781 GTGCTTTCCG GTCTGATGAG TCCGTGAGGA CGAAACAGCC TCTACAAATA ATTTTGTTTA
     3841 AGGCTCGTTG AACACCGTCT CAGGTAAGTA TCAGTTGTAA AAAGAGGGGA AATAGTCCAT
     3901 GTCCAGATTA GATAAAAGTA AAGTGATTAA CAGCGCATTA GAGCTGCTTA ATGAGGTCGG
     3961 AATCGAAGGT TTAACAACCC GTAAACTCGC CCAGAAGCTA GGTGTAGAGC AGCCTACATT
     4021 GTATTGGCAT GTAAAAAATA AGCGGGCTTT GCTCGACGCC TTAGCCATTG AGATGTTAGA
     4081 TAGGCACCAT ACTCACTTTT GCCCTTTAGA AGGGGAAAGC TGGCAAGATT TTTTACGTAA
     4141 TAACGCTAAA AGTTTTAGAT GTGCTTTACT AAGTCATCGC GATGGAGCAA AAGTACATTT
     4201 AGGTACACGG CCTACAGAAA AACAGTATGA AACTCTCGAA AATCAATTAG CCTTTTTATG
     4261 CCAACAAGGT TTTTCACTAG AGAATGCATT ATATGCACTC AGCGCTGTGG GGCATTTTAC
     4321 TTTAGGTTGC GTATTGGAAG ATCAAGAGCA TCAAGTCGCT AAAGAAGAAA GGGAAACACC
     4381 TACTACTGAT AGTATGCCGC CATTATTACG ACAAGCTATC GAATTATTTG ATCACCAAGG
     4441 TGCAGAGCCA GCCTTCTTAT TCGGCCTTGA ATTGATCATA TGCGGATTAG AAAAACAACT
     4501 TAAATGTGAA AGTGGGTCCT GAGGCTCGGT ATCTCGTGGT CTGACGGTAA AATCTATTGT
     4561 AAAAGAGGAG AAATAGTCCA TGCGTAAAGG CGAAGAGCTG TTCACTGGTG TCGTCCCTAT
     4621 TCTGGTGGAA CTGGATGGTG ATGTCAACGG TCATAAGTTT TCCGTGCGTG GCGAGGGTGA
     4681 AGGTGACGCA ACTAATGGTA AACTGACGCT GAAGTTCATC TGTACTACTG GTAAACTGCC
     4741 GGTACCTTGG CCGACTCTGG TAACGACGCT GACTTATGGT GTTCAGTGCT TTGCTCGTTA
     4801 TCCGGACCAT ATGAAGCAGC ATGACTTCTT CAAGTCCGCC ATGCCGGAAG GCTATGTGCA
     4861 GGAACGCACG ATTTCCTTTA AGGATGACGG CACGTACAAA ACGCGTGCGG AAGTGAAATT
     4921 TGAAGGCGAT ACTCTGGTAA ACCGCATTGA GCTGAAAGGC ATTGACTTTA AAGAAGACGG
     4981 CAATATCCTG GGCCATAAGC TGGAATACAA TTTTAACAGC CACAATGTTT ACATCACCGC
     5041 CGATAAACAA AAAAATGGCA CTAAAGCGAA TTTTAAAATT CGCCACAACG TGGAGGATGG
     5101 CAGCGTGCAG CTGGCTGATC ACTACCAGCA AAACACTCCA ATCGGTGATG GTCCTGTTCT
     5161 GCTGCCAGAC AATCACTATC TGAGCACGCA AAGCGTTCTG TCTAAAGATC CGAACGAGAA
     5221 ACGCGATCAT ATGGTTCTGC TGGAGTTCGT AACCGCAGCG GGCATCACGC ATGGTATGGA
     5281 TGAACTGTAC GGCTCGTTAC TTACGACACT CCGAGACAGT CAGAGGGTAT TTATTGAACT
     5341 AGTCCGACGA ACAATAAGGC CTCCCTAACG GGGGGCCTTT TTTATTGATA ACAAAAGTGC
     5401 CTACTCTGGA AAATCTTTTT CAGCAGGACG CACTGACCTC CCTATCAGTG ATAGAGATTG
     5461 ACATCCCTAT CAGTGATAGA GATACTGAGC ACAGTAGTCA CCGGCTGTGC TTGCCGGTCT
     5521 GATGAGCCTG TGAAGGCGAA ACTACCTCTA CAAATAATTT TGTTTAAGGC TCGTGTTACT
     5581 ATTGGCTGAG ATAAGGGTAG CAGAAAAAAG AGGAGAAATA GTCCATGGCT GAAGCGCAAA
     5641 ATGATCCCCT GCTGCCGGGA TACTCGTTTA ATGCCCATCT GGTGGCGGGT TTAACGCCGA
     5701 TTGAGGCCAA CGGTTATCTC GATTTTTTTA TCGACCGACC GCTGGGAATG AAAGGTTATA
     5761 TTCTCAATCT CACCATTCGC GGTCAGGGGG TGGTGAAAAA TCAGGGACGA GAATTTGTTT
     5821 GCCGACCGGG TGATATTTTG CTGTTCCCGC CAGGAGAGAT TCATCACTAC GGTCGTCATC
     5881 CGGAGGCTCG CGAATGGTAT CACCAGTGGG TTTACTTTCG TCCGCGCGCC TACTGGCATG
     5941 AATGGCTTAA CTGGCCGTCA ATATTTGCCA ATACGGGGTT CTTTCGCCCG GATGAAGCGC
     6001 ACCAGCCGCA TTTCAGCGAC TTTTTTGGGC AAATCATTAA CGCCGGGCAA GGGGAAGGGC
     6061 GCTATTCGGA GCTGCTGGCG ATAAATCTGC TTGAGCAATT GTTACTGCGG CGCATGCTAG
     6121 CGATTAACGG ATCGCTCCAT CCACCGATGG ATAATCGGGT ACGCGAGGCT TGTCAGTACA
     6181 TCAGCGATCA CCTGGCAGAC AGCAATTTTG ATATCGCCAG CGTCGCACAG CATGTTTGCT
     6241 TGTCGCCGTC GCGTCTGTCA CATCTTTTCC GCCAGCAGTT AGGGATTAGC GTCTTAAGCT
     6301 GGCGCGAGGA CCAACGTATC AGCCAGGCGA AGCTGCTTTT GAGCACCACC CGGATGCCTA
     6361 TCGCCACCGT CGGTCGCAAT GTTGGTTTTG ACGATCAACT CTATTTCTCG CGGGTATTTA
     6421 AAAAATGCAC CGGGGCCAGC CCGAGCGAGT TCCGTGCCGG TTTGGAAGAA AAAGTGAATG
     6481 ATGTAGCCGT CAAGTTGTCA TGAGGCTCGG TATCTCGTGG TCTGACGGTA AAATCTATTG
     6541 TAAAAGAGGA GAAATAGTCC ATGTCAGAAT TAATTAAAGA AAATATGCAC ATGAAATTAT
     6601 ATATGGAAGG TACTGTCAAC AATCATCATT TCAAATGCAC ATCCGAAGGT GAAGGTAAAC
     6661 CATATGAAGG CACACAAACA ATGCGCATCA AAGCAGTTGA AGGTGGACCC CTGCCCTTTG
     6721 CGTTTGACAT TCTCGCAACG AGCTTTATGT ACGGGTCTAA AACTTTTATC AATCACACCC
     6781 AAGGCATTCC TGACTTTTTT AAACAGTCCT TTCCTGAAGG CTTTACCTGG GAACGTGTAA
     6841 CAACTTATGA AGATGGCGGT GTACTTACAG CAACTCAAGA TACGAGTTTA CAAGATGGCT
     6901 GTCTGATTTA CAATGTTAAA ATCCGTGGCG TAAATTTCCC GAGTAACGGA CCCGTAATGC
//