LOCUS       pVCS7                   6388 bp ds-DNA     circular     22-NOV-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     Linker          5..51
                     /label="LMP"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     regulatory      56..116
                     /label="Terminator1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    117..136
                     /label="SpacerA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Repressor       137..226
                     /label="CymR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    227..301
                     /label="RiboA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Linker          308..339
                     /label="UTR2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Ribosomal Bi... 341..354
                     /label="RBS3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Repressor       359..1093
                     /label="VanR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     Linker          1100..1131
                     /label="UTR3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Ribosomal Bi... 1133..1146
                     /label="RBS3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Reporter        1151..1846
                     /label="mKate2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Linker          1853..1897
                     /label="L1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      1902..1962
                     /label="Terminator1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1963..1982
                     /label="SpacerA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Promoter        1983..2032
                     /label="pVan"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2033..2107
                     /label="RiboA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Linker          2114..2145
                     /label="UTR2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Ribosomal Bi... 2147..2160
                     /label="RBS1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Repressor       2165..2776
                     /label="CymR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Linker          2783..2814
                     /label="UTR3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Ribosomal Bi... 2816..2829
                     /label="RBS3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Reporter        2834..3544
                     /label="superfolder GFP"
                     /ApEinfo_revcolor="#a2d39c"
                     /ApEinfo_fwdcolor="#a2d39c"
     Linker          3551..3597
                     /label="LMS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Terminator      3608..3710
                     /label="SEVA_T0"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     Selection Ma... 3737..4663
                     /label="SEVA_Km"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Misc            4674..4736
                     /label="oriT Replacement Spacer"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     misc_feature    4737..6258
                     /label="SEVA_pBBR1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Terminator      6273..6377
                     /label="SEVA_T1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 CTCGGGTAAG AACTCGCACT TCGTGGAAAC ACTATTATCT GGTGGGTCTC TGTCCCTCGG
       61 TACCAAATTC CAGAAAAGAG GCCTCCCGAA AGGGGGGCCT TTTTTCGTTT TGGTCCGTGC
      121 CTACTCTGGA AAATCTAACA AACAGACAAT CTGGTCTGTT TGTATTATGG AAAATTTTTC
      181 TGTATAATAG ATTCAACAAA CAGACAATCT GGTCTGTTTG TATTATAGCT GTCACCGGAT
      241 GTGCTTTCCG GTCTGATGAG TCCGTGAGGA CGAAACAGCC TCTACAAATA ATTTTGTTTA
      301 AGGCTCGTGT TACTATTGGC TGAGATAAGG GTAGCAGAAA AAAGAGGAGA AATAGTCCAT
      361 GGACATGCCT CGTATTAAAC CGGGTCAGCG TGTTATGATG GCACTGCGTA AAATGATTGC
      421 AAGCGGTGAA ATCAAAAGTG GTGAACGTAT TGCAGAAATT CCGACCGCAG CAGCACTGGG
      481 TGTTAGCCGT ATGCCGGTTC GTATCGCACT GCGTTCACTG GAACAAGAAG GTCTGGTTGT
      541 TCGTCTGGGT GCACGTGGTT ATGCAGCCCG TGGTGTTAGC AGCGATCAGA TTCGTGATGC
      601 AATTGAAGTT CGTGGTGTTC TGGAAGGTTT TGCAGCACGT CGTCTGGCAG AACGTGGTAT
      661 GACCGCAGAA ACCCATGCAC GTTTTGTTGT ACTGATTGCA GAAGGTGAAG CACTGTTTGC
      721 AGCCGGTCGC CTGAATGGTG AAGATCTGGA TCGTTATGCC GCATATAATC AGGCATTTCA
      781 TGATACCCTG GTTAGCGCAG CAGGTAATGG TGCAGTTGAA AGCGCACTGG CACGTAATGG
      841 TTTTGAACCG TTTGCAGCAG CCGGTGCACT GGCCCTGGAT CTGATGGACC TGTCTGCCGA
      901 ATATGAACAT CTGCTGGCAG CACATCGTCA GCATCAGGCA GTTCTGGATG CAGTTAGCTG
      961 TGGTGATGCC GAAGGTGCAG AACGTATTAT GCGTGATCAT GCACTGGCAG CAATTCGTAA
     1021 TGCAAAAGTT TTTGAAGCAG CAGCAAGCGC AGGCGCACCG CTGGGTGCAG CATGGTCAAT
     1081 TCGTGCAGAT TGAGGCTCGG TATCTCGTGG TCTGACGGTA AAATCTATTG TAAAAGAGGA
     1141 GAAATAGTCC ATGTCAGAAT TAATTAAAGA AAATATGCAC ATGAAATTAT ATATGGAAGG
     1201 TACTGTCAAC AATCATCATT TCAAATGCAC ATCCGAAGGT GAAGGTAAAC CATATGAAGG
     1261 CACACAAACA ATGCGCATCA AAGCAGTTGA AGGTGGACCC CTGCCCTTTG CGTTTGACAT
     1321 TCTCGCAACG AGCTTTATGT ACGGGTCTAA AACTTTTATC AATCACACCC AAGGCATTCC
     1381 TGACTTTTTT AAACAGTCCT TTCCTGAAGG CTTTACCTGG GAACGTGTAA CAACTTATGA
     1441 AGATGGCGGT GTACTTACAG CAACTCAAGA TACGAGTTTA CAAGATGGCT GTCTGATTTA
     1501 CAATGTTAAA ATCCGTGGCG TAAATTTCCC GAGTAACGGA CCCGTAATGC AAAAAAAAAC
     1561 TCTTGGTTGG GAAGCATCAA CAGAAACCTT ATATCCTGCG GACGGTGGCT TAGAAGGACG
     1621 CGCAGACATG GCACTGAAAT TAGTTGGAGG CGGTCATTTA ATCTGCAACC TGAAAACAAC
     1681 CTATCGTTCC AAAAAACCCG CTAAAAACCT TAAAATGCCT GGAGTATACT ATGTTGATCG
     1741 TCGCTTAGAA CGTATTAAAG AAGCTGATAA AGAAACCTAC GTTGAACAAC ATGAAGTAGC
     1801 CGTAGCCCGT TATTGTGACC TTCCGTCGAA ATTAGGACAT CGTTGAGGCT CGTTACTTAC
     1861 GACACTCCGA GACAGTCAGA GGGTATTTAT TGAACTAGTC CCTCGGTACC AAATTCCAGA
     1921 AAAGAGGCCT CCCGAAAGGG GGGCCTTTTT TCGTTTTGGT CCGTGCCTAC TCTGGAAAAT
     1981 CTATTGGATC CAATTGACAG CTAGCTCAGT CCTAGGTACC ATTGGATCCA ATAGCTGTCA
     2041 CCGGATGTGC TTTCCGGTCT GATGAGTCCG TGAGGACGAA ACAGCCTCTA CAAATAATTT
     2101 TGTTTAAGGC TCGTGTTACT ATTGGCTGAG ATAAGGGTAG CAGAAAATCA CACAGGACTA
     2161 GTCCATGAGC CCGAAACGTC GTACCCAGGC AGAACGTGCA ATGGAAACCC AGGGTAAACT
     2221 GATTGCAGCA GCACTGGGTG TTCTGCGTGA AAAAGGTTAT GCAGGTTTTC GTATTGCAGA
     2281 TGTTCCGGGT GCAGCCGGTG TTAGCCGTGG TGCACAGAGC CATCATTTTC CGACCAAACT
     2341 GGAACTGCTG CTGGCAACCT TTGAATGGCT GTATGAGCAG ATTACCGAAC GTAGCCGTGC
     2401 ACGTCTGGCA AAACTGAAAC CGGAAGATGA TGTTATTCAG CAGATGCTGG ATGATGCAGC
     2461 AGAATTTTTT CTGGATGATG ATTTTAGCAT CGGCCTGGAT CTGATTGTTG CAGCAGATCG
     2521 TGATCCGGCA CTGCGTGAAG GTATTCAGCG TACCGTTGAA CGTAATCGTT TTGTTGTTGA
     2581 AGATATGTGG CTGGGTGTGC TGGTGAGCCG TGGTCTGAGC CGTGATGATG CCGAAGATAT
     2641 TCTGTGGCTG ATTTTTAACA GCGTTCGTGG TCTGGTAGTT CGTAGCCTGT GGCAGAAAGA
     2701 TAAAGAACGT TTTGAACGTG TGCGTAATAG CACCCTGGAA ATTGCACGTG AACGTTATGC
     2761 AAAATTCAAA CGTTGAGGCT CGGTATCTCG TGGTCTGACG GTAAAATCTA TTGTAAAAGA
     2821 GGAGAAATAG TCCATGCGTA AAGGCGAAGA GCTGTTCACT GGTGTCGTCC CTATTCTGGT
     2881 GGAACTGGAT GGTGATGTCA ACGGTCATAA GTTTTCCGTG CGTGGCGAGG GTGAAGGTGA
     2941 CGCAACTAAT GGTAAACTGA CGCTGAAGTT CATCTGTACT ACTGGTAAAC TGCCGGTACC
     3001 TTGGCCGACT CTGGTAACGA CGCTGACTTA TGGTGTTCAG TGCTTTGCTC GTTATCCGGA
     3061 CCATATGAAG CAGCATGACT TCTTCAAGTC CGCCATGCCG GAAGGCTATG TGCAGGAACG
     3121 CACGATTTCC TTTAAGGATG ACGGCACGTA CAAAACGCGT GCGGAAGTGA AATTTGAAGG
     3181 CGATACTCTG GTAAACCGCA TTGAGCTGAA AGGCATTGAC TTTAAAGAAG ACGGCAATAT
     3241 CCTGGGCCAT AAGCTGGAAT ACAATTTTAA CAGCCACAAT GTTTACATCA CCGCCGATAA
     3301 ACAAAAAAAT GGCACTAAAG CGAATTTTAA AATTCGCCAC AACGTGGAGG ATGGCAGCGT
     3361 GCAGCTGGCT GATCACTACC AGCAAAACAC TCCAATCGGT GATGGTCCTG TTCTGCTGCC
     3421 AGACAATCAC TATCTGAGCA CGCAAAGCGT TCTGTCTAAA GATCCGAACG AGAAACGCGA
     3481 TCATATGGTT CTGCTGGAGT TCGTAACCGC AGCGGGCATC ACGCATGGTA TGGATGAACT
     3541 GTACGGCTCG GGAGACCTAT CGGTAATAAC AGTCCAATCT GGTGTAACTT CGGAATCGTC
     3601 CACTAGTCTT GGACTCCTGT TGATAGATCC AGTAATGACC TCAGAACTCC ATCTGGATTT
     3661 GTTCAGAACG CTCGGTTGCC GCCGGGCGTT TTTTATTGGT GAGAATCCAG GGGTCCCCAA
     3721 TAATTACGAT TTAAATTTGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT
     3781 ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG
     3841 AGCCATATTC AGCGTGAAAC GAGCTGTAGC CGTCCGCGTC TGAACAGCAA CATGGATGCG
     3901 GATCTGTATG GCTATAAATG GGCGCGTGAT AACGTGGGTC AGAGCGGCGC GACCATTTAT
     3961 CGTCTGTATG GCAAACCGGA TGCGCCGGAA CTGTTTCTGA AACATGGCAA AGGCAGCGTG
     4021 GCGAACGATG TGACCGATGA AATGGTGCGT CTGAACTGGC TGACCGAATT TATGCCGCTG
     4081 CCGACCATTA AACATTTTAT TCGCACCCCG GATGATGCGT GGCTGCTGAC CACCGCGATT
     4141 CCGGGCAAAA CCGCGTTTCA GGTGCTGGAA GAATATCCGG ATAGCGGCGA AAACATTGTG
     4201 GATGCGCTGG CCGTGTTTCT GCGTCGTCTG CATAGCATTC CGGTGTGCAA CTGCCCGTTT
     4261 AACAGCGATC GTGTGTTTCG TCTGGCCCAG GCGCAGAGCC GTATGAACAA CGGCCTGGTG
     4321 GATGCGAGCG ATTTTGATGA TGAACGTAAC GGCTGGCCGG TGGAACAGGT GTGGAAAGAA
     4381 ATGCATAAAC TGCTGCCGTT TAGCCCGGAT AGCGTGGTGA CCCACGGCGA TTTTAGCCTG
     4441 GATAACCTGA TTTTCGATGA AGGCAAACTG ATTGGCTGCA TTGATGTGGG CCGTGTGGGC
     4501 ATTGCGGATC GTTATCAGGA TCTGGCCATT CTGTGGAACT GCCTGGGCGA ATTTAGCCCG
     4561 AGCCTGCAAA AACGTCTGTT TCAGAAATAT GGCATTGATA ATCCGGATAT GAACAAACTG
     4621 CAATTTCATC TGATGCTGGA TGAATTTTTC TAATAATTAA TTGGACCGCG GTCGGCTCGA
     4681 CCCACGACTA TTGACTGCTC TGAGAAAGTT GATTGTTACG ATTAGTCCGG CCGGCCCTAC
     4741 CGGCGCGGCA GCGTTACCCG TGTCGGCGGC TCCAACGGCT CGCCATCGTC CAGAAAACAC
     4801 GGCTCATCGG GCATCGGCAG GCGCTGCTGC CCGCGCCGTT CCCATTCCTC CGTTTCGGTC
     4861 AAGGCTGGCA GGTCTGGTTC CATGCCCGGA ATGCCGGGCT GGCTGGGCGG CTCCTCGCCG
     4921 GGGCCGGTCG GTAGTTGCTG CTCGCCCGGA TACAGGGTCG GGATGCGGCG CAGGTCGCCA
     4981 TGCCCCAACA GCGATTCGTC CTGGTCGTCG TGATCAACCA CCACGGCGGC ACTGAACACC
     5041 GACAGGCGCA ACTGGTCGCG GGGCTGGCCC CACGCCACGC GGTCATTGAC CACGTAGGCC
     5101 GACACGGTGC CGGGGCCGTT GAGCTTCACG ACGGAGATCC AGCGCTCGGC CACCAAGTCC
     5161 TTGACTGCGT ATTGGACCGT CCGCAAAGAA CGTCCGATGA GCTTGGAAAG TGTCTTCTGG
     5221 CTGACCACCA CGGCGTTCTG GTGGCCCATC TGCGCCACGA GGTGATGCAG CAGCATTGCC
     5281 GCCGTGGGTT TCCTCGCAAT AAGCCCGGCC CACGCCTCAT GCGCTTTGCG TTCCGTTTGC
     5341 ACCCAGTGAC CGGGCTTGTT CTTGGCTTGA ATGCCGATTT CTCTGGACTG CGTGGCCATG
     5401 CTTATCTCCA TGCGGTAGGG GTGCCGCACG GTTGCGGCAC CATGCGCAAT CAGCTGCAAC
     5461 TTTTCGGCAG CGCGACAACA ATTATGCGTT GCGTAAAAGT GGCAGTCAAT TACAGATTTT
     5521 CTTTAACCTA CGCAATGAGC TATTGCGGGG GGTGCCGCAA TGAGCTGTTG CGTACCCCCC
     5581 TTTTTTAAGT TGTTGATTTT TAAGTCTTTC GCATTTCGCC CTATATCTAG TTCTTTGGTG
     5641 CCCAAAGAAG GGCACCCCTG CGGGGTTCCC CCACGCCTTC GGCGCGGCTC CCCCTCCGGC
     5701 AAAAAGTGGC CCCTCCGGGG CTTGTTGATC GACTGCGCGG CCTTCGGCCT TGCCCAAGGT
     5761 GGCGCTGCCC CCTTGGAACC CCCGCACTCG CCGCCGTGAG GCTCGGGGGG CAGGCGGGCG
     5821 GGCTTCGCCC TTCGACTGCC CCCACTCGCA TAGGCTTGGG TCGTTCCAGG CGCGTCAAGG
     5881 CCAAGCCGCT GCGCGGTCGC TGCGCGAGCC TTGACCCGCC TTCCACTTGG TGTCCAACCG
     5941 GCAAGCGAAG CGCGCAGGCC GCAGGCCGGA GGCTTTTCCC CAGAGAAAAT TAAAAAAATT
     6001 GATGGGGCAA GGCCGCAGGC CGCGCAGTTG GAGCCGGTGG GTATGTGGTC GAAGGCTGGG
     6061 TAGCCGGTGG GCAATCCCTG TGGTCAAGCT CGTGGGCAGG CGCAGCCTGT CCATCAGCTT
     6121 GTCCAGCAGG GTTGTCCACG GGCCGAGCGA AGCGAGCCAG CCGGTGGCCG CTCGCGGCCA
     6181 TCGTCCACAT ATCCACGGGC TGGCAAGGGA GCGCAGCGAC CGCGCAGGGC GAAGCCCGGA
     6241 GAGCAAGCCC GTAGGGGGGG CGCGCCCAGC TGTCTAGGGC GGCGGATTTG TCCTACTCAG
     6301 GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTCG ACTGAGCCTT
     6361 TCGTTTTATT TGATGCCTTT AATTAAGG
//