LOCUS       Exported                6860 bp ds-DNA     circular SYN 21-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 4, 2024 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1015..3162
                     /codon_start=1
                     /label=ZDHHC5
                     /translation="MPAESGKRFKPSKYVPVSAAAIFLVGATTLFFAFTCPGLSLYVSP
                     AVPIYNAIMFLFVLANFSMATFMDPGIFPRAEEDEDKEDDFRAPLYKTVEIKGIQVRMK
                     WCATCRFYRPPRCSHCSVCDNCVEEFDHHCPWVNNCIGRRNYRYFFLFLLSLTAHIMGV
                     FGFGLLYVLYHIEELSGVRTAVTMAVMCVAGLFFIPVAGLTGFHVVLVARGRTTNEQVT
                     GKFRGGVNPFTNGCCNNVSRVLCSSPAPRYLGRPKKEKTIVIRPPFLRPEVSDGQITVK
                     IMDNGIQGELRRTKSKGSLEITESQSADAEPPPPPKPDLSRYTGLRTHLGLATNEDSSL
                     LAKDSPPTPTMYKYRPGYSSSSTSAAMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSE
                     PSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKS
                     LANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPR
                     LVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPG
                     SGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSM
                     SYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSS
                     PTRGGVKKVSGVGGTTYEISV"
     polyA_signal    3201..3823
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        3852..4181
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4032..4167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4220..4238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4252..4268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4276..4292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4300..4330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4345..4366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4654..5242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5413..6273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6274..6378)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6405..6860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcaatgccc
     1021 gcagagtctg gaaagagatt caaacccagc aagtatgtcc cggtctctgc agccgccatc
     1081 ttcctagtgg gagctacgac actcttcttt gcctttacgt gtccaggact aagcctgtat
     1141 gtgtcacctg cagtgcccat ctacaatgca attatgtttc tctttgtgtt ggccaacttc
     1201 agcatggcca ccttcatgga cccagggatt ttccctcgag ctgaggagga tgaggacaag
     1261 gaagatgatt tccgagctcc cctttacaaa acagtggaga taaagggcat ccaggtgcgc
     1321 atgaaatggt gtgccacctg ccgcttttac cgtccccctc gatgttccca ctgcagtgtc
     1381 tgtgacaact gtgtggagga atttgatcat cactgcccct gggtgaataa ctgtattggt
     1441 cgccggaact accgttattt tttccttttc ctcctttccc tgacagccca cattatgggt
     1501 gtgtttggct ttggcctcct ttatgtcctc taccacatag aggaactctc aggggtccgc
     1561 acggctgtca caatggcagt aatgtgtgtg gctggcttat tcttcatccc tgtagctggc
     1621 ctcacgggat ttcacgtggt tctggtggcc aggggacgca caaccaatga acaggttacg
     1681 ggtaaattcc ggggaggtgt gaaccccttc accaatggct gctgtaacaa tgtcagccgt
     1741 gttctctgca gttctccagc acccaggtat ttggggagac caaagaaaga gaagacaatt
     1801 gtaatcagac ctcccttcct tcgaccagaa gtttcagatg ggcagataac tgtgaagatc
     1861 atggataatg gcatccaggg agagctgagg agaacaaagt ctaagggaag cctggagata
     1921 acagagagcc agtctgcaga tgctgaacct ccacctcctc ctaagccaga cctgagccgt
     1981 tacacagggt tgcgaacaca cctcggcttg gctactaatg aggatagtag cttattggcc
     2041 aaggacagcc ccccgacacc taccatgtac aagtatcggc cgggttacag tagcagcagt
     2101 acgtcagctg ccatgccgca ttcctccagc gccaagttga gtcgtgggga cagcttgaag
     2161 gagccaacct caattgcaga gagcagccgt caccccagct accgctcaga gcccagcttg
     2221 gaaccagaga gcttccgttc tcctaccttt ggcaaaagtt ttcacttcga tccactatcc
     2281 agtggctcac gctcctccag cctcaagtca gcccagggca caggctttga gctgggccag
     2341 ttgcaatcca ttcgttcaga gggcaccacc tccacctcct ataagagcct ggccaaccag
     2401 acacgcaatg gaagcctatc ttatgacagc ttgctcacac cttcagacag ccctgatttt
     2461 gagtcagtgc aggcagggcc tgagccagac ccacctttag gctatacctc tcccttcctg
     2521 tcagccaggc tggcccagca acgggaagct gagaggcacc cacgtttggt gccaactggc
     2581 ccaacacacc gagagccctc accagtccgt tacgacaatc tgtcgcgcca cattgtggcc
     2641 tctctccagg aacgagagaa gttgctgcgc cagtcacccc cactcccggg ccgtgaggaa
     2701 gaaccaggct tgggggactc aggcattcag tcaacaccag gctcgggcca tgcccctcgt
     2761 actagttcct cctcagatga ttcaaagaga tcacctttgg gcaagactcc actgggacgc
     2821 ccagctgtcc cccgttttgg caagccagat gggctaaggg gccggggagt agggtcccct
     2881 gaaccaggcc caacagcccc atacctgggc cgatcgatgt cttacagcag ccaaaaagcc
     2941 caacctggtg tctctgagac agaagaagtg gccttgcagc cattactgac acccaaagat
     3001 gaagtacagc tgaagaccac ctacagcaaa tccaacgggc agcccaagag cttaggctca
     3061 gcctcccctg gcccaggcca gccacctctc agtagcccca cgaggggagg agtcaagaag
     3121 gtgtcagggg ttggtggtac cacctatgag atttcggtgt gaagatctga tatcggtacc
     3181 agtcgactct agaggatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     3241 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     3301 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     3361 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     3421 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     3481 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3541 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     3601 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     3661 ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac
     3721 agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg
     3781 tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg cggccagctt
     3841 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3901 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3961 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4021 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4081 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4141 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcctc gaggaactga
     4201 aaaaccagaa agttaattcc ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct
     4261 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4321 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4381 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4441 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4501 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4561 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5281 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5341 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5401 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5461 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5521 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5581 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5641 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5701 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5761 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5821 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5881 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5941 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6001 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6061 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6121 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6181 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6241 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6301 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6361 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     6421 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6481 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6541 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6601 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6661 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6721 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6781 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6841 aatattaacg cttacaattt
//
