LOCUS       Exported                6581 bp ds-DNA     circular SYN 08-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 4, 2024 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1015..2883
                     /codon_start=1
                     /label=ZDHHC13
                     /translation="MEGPGLGSQCRNHSHGPHPPGFGRYGICAHENKELANAREALPLI
                     EDSSNCDIVKATQYGIFERCKELVEAGYDVRQPDKENVSLLHWAAINNRLDLVKFYISK
                     GAVVDQLGGDLNSTPLHWAIRQGHLPMVILLLQHGADPTLIDGEGFSSIHLAVLFQHMP
                     IIAYLISKGQSVNMTDVNGQTPLMLSAHKVIGPEPTGFLLKFNPSLNVVDKIHQNTPLH
                     WAVAAGNVNAVDKLLEAGSSLDIQNVKGETPLDMALQNKNQLIIHMLKTEAKMRANQKF
                     RLWRWLQKCELFLLLMLSVITMWAIGYILDFNSDSWLLKGCLLVTLFFLTSLFPRFLVG
                     YKNLVYLPTAFLLSSVFWIFMTWFILFFPDLAGAPFYFSFIFSIVAFLYFFYKTWATDP
                     GFTKASEEEKKVNIITLAETGSLDFRTFCTSCLIRKPLRSLHCHVCNCCVARYDQHCLW
                     TGRCIGFGNHHYYIFFLFFLSMVCGWIIYGSFIYLSSHCATTFKEDGLWTYLNQIVACS
                     PWVLYILMLATFHFSWSTFLLLNQLFQIAFLGLTSHERISLQKQSKHMKQTLSLRKTPY
                     NLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRSV"
     polyA_signal    2922..3544
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        3573..3902
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3753..3888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3941..3959)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3973..3989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3997..4013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4021..4051)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4066..4087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4375..4963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5134..5994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5995..6099)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6126..6581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcaatggag
     1021 gggccggggc tgggctcgca gtgcaggaat cacagccatg gcccccaccc tccaggattt
     1081 ggtcgatatg gcatctgtgc acatgaaaac aaagaacttg ccaatgcaag agaagctctt
     1141 cctcttatag aggactctag taactgtgac attgtcaaag ctactcaata cggaattttt
     1201 gaacgatgta aagagttggt agaagcagga tatgatgtca ggcaaccaga taaagaaaat
     1261 gtgtcgcttc ttcattgggc tgctattaac aacagactgg atcttgtaaa gttttatatt
     1321 tcaaaaggtg ctgttgtaga tcagttgggt ggagatttaa attcaactcc tcttcactgg
     1381 gccatccgac aaggacattt acctatggtc atattattac tccagcatgg tgcagacccc
     1441 actcttattg atggagaggg attcagcagc atccacctgg cagtattgtt tcaacacatg
     1501 cctattatag catatctcat ctcaaaggga cagagtgtga atatgacaga tgtaaatggg
     1561 cagacacctc tcatgttatc agctcacaaa gtaattgggc cagaaccaac tggatttctt
     1621 ttaaagttta atccttctct caatgtggtt gataaaatac accaaaacac tccacttcac
     1681 tgggcagttg cagcaggaaa tgttaatgca gttgataagc ttttggaagc tggttctagc
     1741 ctggatatcc agaatgttaa gggagaaaca cctcttgata tggctctaca aaacaaaaat
     1801 cagctcatta ttcatatgct aaaaacagaa gccaaaatga gagccaacca aaagttcaga
     1861 ctttggaggt ggctgcagaa atgcgagctc ttcctgctgc tgatgctttc tgtgattacc
     1921 atgtgggcta ttggatacat attggacttc aattcagatt cttggctttt aaaaggatgt
     1981 cttctagtaa cactgttttt tctgacatct ttgtttccaa ggttcttggt tgggtataag
     2041 aaccttgtat acttaccaac agcctttctg ctaagttctg ttttttggat atttatgact
     2101 tggttcatct tattttttcc tgatttagca ggagcccctt tctatttcag tttcattttc
     2161 agcatagtag cctttctata ctttttctat aagacttggg caactgatcc aggcttcact
     2221 aaggcttctg aagaagaaaa gaaagtgaat atcatcaccc ttgcagaaac tggctctctg
     2281 gacttcagaa cattttgtac atcatgtctt ataaggaagc cattaaggtc actccactgc
     2341 catgtatgca actgctgtgt ggctcgatat gatcaacact gcctgtggac tggacggtgc
     2401 ataggttttg gcaaccatca ctattacata ttcttcttgt ttttcctttc catggtatgt
     2461 ggctggatta tatatggatc tttcatctat ttgtccagtc attgtgccac aacattcaaa
     2521 gaagatggat tatggactta cctcaatcag attgtggcct gttccccttg ggttttatat
     2581 atcttgatgc tagcaacttt ccatttctca tggtcaacat ttttattatt aaatcaactc
     2641 tttcagattg cctttctggg cctgacctcc catgagagaa tcagcctgca gaagcagagc
     2701 aagcatatga aacagacgtt gtccctcagg aagacaccat acaatcttgg attcatgcag
     2761 aacctggcag atttctttca gtgtggctgc tttggcttgg tgaagccctg tgtggtagat
     2821 tggacatcac agtacaccat ggtctttcac ccagccaggg agaaggttct tcgctcagta
     2881 tgaagatctg atatcggtac cagtcgactc tagaggatcc cgggtggcat ccctgtgacc
     2941 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     3001 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     3061 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     3121 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     3181 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     3241 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     3301 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     3361 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc
     3421 agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt
     3481 tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg
     3541 aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3601 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3661 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3721 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3781 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3841 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3901 aaaagctcct cgaggaactg aaaaaccaga aagttaattc cctatagtga gtcgtattaa
     3961 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6121 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6541 atttaacgcg aattttaaca aaatattaac gcttacaatt t
//
