LOCUS       pCMV-mGold2s-Actin-C-18 5778 bp ds-DNA     circular     05-DEC-2024
DEFINITION  .
KEYWORDS    "accession:addgene_56395_226587"
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported from database: AddGene Entry: mPapaya-Actin-C-18 URL:
            https://www.addgene.org/browse/sequence/226587/ GENERAL Depositing
            Lab: Michael Davidson Publication: Hoi et al Chem Biol. 2013 Oct
            24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013
            Oct 3. Purpose: Localization: Actin, Excitation: 530, Emission: 541
            BACKBONE Backbone size w/o insert (bp): 4750 Selectable markers:
            Neomycin (select with G418) Vector backbone: mPapaya-C1 Vector
            type: Mammalian Expression CLONING INFORMATION 3′ sequencing
            primer: SV40pA-R 5′ sequencing primer: CMV-F Cloning method:
            Unknown GROWTH IN BACTERIA Bacterial Resistance(s): Kanamycin Copy
            number: High Copy Growth Strain(s): DH5alpha Growth Temperature:
            37°C GENE/INSERT Alt name: NM_001101.3 Entrez Gene:  Gene/Insert
            name: Actin Promoter: CMV Species: H. sapiens (human) Tag / Fusion
            Protein:
FEATURES             Location/Qualifiers
     source          1..5778
                     /label="source"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     enhancer        1..304
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        305..508
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     459..479
                     /label="CMV-F"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    525..572
                     /label="5559_F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      547..552
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    553..572
                     /label="5555_F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    553..572
                     /label="5558_F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     source          553..1269
                     /label="source"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     source          553..1269
                     /label="source"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             553..1269
                     /label="mGold2s"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     source          553..1269
                     /label="source"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     source          553..1269
                     /label="source"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             553..1269
                     /label="Translation 553-1269"
                     /translation="MASKGEELFTGVVPILVELDGDINGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTSLGYGLQCFARYPDNMKRHDFFKSAMPEGYVQERTIFFEDDGNYKTRAEVKFEGGTLVNRVELKGIDFKEDGNILGHKLEYNYNCHNVFITADRQQNGIKVNFKIRHNIEVGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLSMDELYK"
     CDS             856..858
                     /label="Translation 856-858"
                     /translation="E"
     CDS             1072..1074
                     /label="Translation 1072-1074"
                     /translation="V"
     misc_feature    complement(1250..1269)
                     /label="3696_R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1250..1281)
                     /label="3681_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    2578..2699
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(2615..2634)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2669..2688
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      complement(2706..3161)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2843..2864)
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     3055..3074
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        3188..3292
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(3317..3337)
                     /label="pBABE 3'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        3361..3571
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3383..3579
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      3430..3565
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     3492..3511
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             3614..4408
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(3668..3687)
                     /label="Neo-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     4278..4297
                     /label="Neo-F"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4596..4615)
                     /label="TK-pA-R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    4640..4687
                     /label="HSV TK poly(A) signal"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      5016..5604
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     5505..5524
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccATGGCGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG
      601 GTCGAGCTGG ACGGCGACAT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC
      661 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG
      721 CCCTGGCCCA CCCTCGTGAC CAGCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC
      781 GACAACATGA AGCGGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG
      841 CGCACCATCT TCTTCGAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTTGAG
      901 GGCGGCACCC TGGTGAACCG CGTCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC
      961 ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACTGCCACA ACGTCTTTAT CACCGCCGAC
     1021 AGGCAGCAGA ACGGCATCAA GGTCAACTTC AAGATCCGCC ACAACATCGA GGTCGGCGGC
     1081 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG
     1141 CCCGACAACC ACTACCTGAG CTACCAGTCC AAGCTGAGCA AAGACCCCAA CGAGAAGCGC
     1201 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCAG CATGGACGAG
     1261 CTGTACAAGt ccggactcag atctggcagc ggtggaggca gcgcatccgg cggaagcgga
     1321 agcgatgatg atatcgccgc gctcgtcgtc gacaacggct ccggcatgtg caaggccggc
     1381 ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca tcgtggggcg ccccaggcac
     1441 cagggcgtga tggtgggcat gggtcagaag gattcctatg tgggcgacga ggcccagagc
     1501 aagagaggca tcctcaccct gaagtacccc atcgagcacg gcatcgtcac caactgggac
     1561 gacatggaga aaatctggca ccacaccttc tacaatgagc tgcgtgtggc tcccgaggag
     1621 caccccgtgc tgctgaccga ggcccccctg aaccccaagg ccaaccgcga gaagatgacc
     1681 cagatcatgt ttgagacctt caacacccca gccatgtacg ttgctatcca ggctgtgcta
     1741 tccctgtacg cctctggccg taccactggc atcgtgatgg actccggtga cggggtcacc
     1801 cacactgtgc ccatctacga ggggtatgcc ctcccccatg ccatcctgcg tctggacctg
     1861 gctggccggg acctgactga ctacctcatg aagatcctca ccgagcgcgg ctacagcttc
     1921 accaccacgg ccgagcggga aatcgtgcgt gacattaagg agaagctgtg ctacgtcgcc
     1981 ctggacttcg agcaagagat ggccacggct gcttccagct cctccctgga gaagagctac
     2041 gagctgcctg acggccaggt catcaccatt ggcaatgagc ggttccgctg ccctgaggca
     2101 ctcttccagc cttccttcct gggcatggag tcctgtggca tccacgaaac taccttcaac
     2161 tccatcatga agtgtgacgt ggacatccgc aaagacctgt acgccaacac agtgctgtct
     2221 ggcggcacca ccatgtaccc tggcattgcc gacaggatgc agaaggagat cactgccctg
     2281 gcacccagca caatgaagat caagatcatt gctcctcctg agcgcaagta ctccgtgtgg
     2341 atcggcggct ccatcctggc ctcgctgtcc accttccagc agatgtggat cagcaagcag
     2401 gagtatgacg agtccggccc ctccatcgtc caccgcaaat gcttctaggg atccaccgga
     2461 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2521 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2581 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2641 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     2701 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2761 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2821 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2881 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2941 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3001 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3061 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3121 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     3181 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3241 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     3301 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3361 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3421 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3481 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3541 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     3601 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3661 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3721 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3781 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3841 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3901 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3961 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4021 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4081 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4141 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4201 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4261 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4321 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4381 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4441 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4501 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4561 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     4621 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     4681 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4741 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4801 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     4861 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4921 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4981 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5041 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5101 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5161 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5221 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5281 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5341 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5401 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5461 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5521 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5581 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5641 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5701 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     5761 cccatatatg gagttccg
//