LOCUS       pCAG-GPHN-FingR-mGold2s 7500 bp ds-DNA     circular     05-DEC-2024
DEFINITION  .
KEYWORDS    "marker:AmpR" "ref:pmid\:23791193"
COMMENT     <small>[1] Recombinant probes for visualizing endogenous synaptic
            proteins in living neurons. Neuron. 2013 Jun 19;78(6):971-85. doi:
            10.1016/j.neuron.2013.04.017. Gross GG, Junge JA, Mora RJ, Kwon HB,
            Olson CA, Takahashi TT, Liman ER, Ellis-Davies GC, McGee AW,
            Sabatini BL, Roberts RW, Arnold DB (pmid:23791193)</small>
FEATURES             Location/Qualifiers
     protein_bind    24..35
                     /label="ZF binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: CCR5TC zinc-finger domain note: target sequence for the CCR5TC zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 2008; Gross et al., 2013)"
     enhancer        39..418
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        421..696
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     intron          697..1708
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: chimera between introns from chicken β-actin and rabbit β-globin"
     CDS             2056..2079
                     /label="FLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="product: FLAG® epitope tag, followed by an enterokinase cleavage site codon_start: 1 translation: DYKDDDDK transl_table: 1"
     misc_feature    2104..2120
                     /label="5554_F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             2104..2814
                     /label="mGold2s"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="ASKGEELFTGVVPILVELDGDINGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTSLGYGLQCFARYPDNMKRHDFFKSAMPEGYVQERTIFFEDDGNYKTRAEVKFEGGTLVNRVELKGIDFKEDGNILGHKLEYNYNCHNVFITADRQQNGIKVNFKIRHNIEVGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLSMDELY"
     CDS             2404..2406
                     /label="Translation 2404-2406"
                     /translation="E"
     CDS             2620..2622
                     /label="Translation 2620-2622"
                     /translation="V"
     CDS             2860..3201
                     /label="CCR5TC"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="product: zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease that recognizes the left half-site target sequence in the human CCR5 gene (Perez et al., 2008; Gross et al., 2013) codon_start: 1 translation: FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLNSHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRHTKIHLRGSQL transl_table: 1"
     CDS             3250..3375
                     /label="KRAB-A"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="product: Krüppel-associated box (KRAB) transcriptional repression domain from the rat zinc finger protein Kid-1 (Witzgall et al., 1994) codon_start: 1 translation: VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM transl_table: 1"
     intron          3424..3556
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: chimera between introns from human β-globin and immunoglobulin heavy chain genes"
     misc_feature    3565..4153
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: woodchuck hepatitis virus posttranscriptional regulatory element"
     CDS             complement(4036..4047)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="product: Factor Xa recognition and cleavage site codon_start: 1 translation: IEGR transl_table: 1"
     polyA_signal    4186..4297
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     polyA_signal    4589..4644
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: rabbit β-globin polyadenylation signal (Gil and Proudfoot, 1987)"
     primer_bind     complement(5003..5019)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    5027..5043
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5051..5057)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(5058..5075)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(5076..5081)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    5096..5117
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        5176..5371
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 early promoter"
     rep_origin      5222..5357
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     polyA_signal    5377..5511
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: SV40 polyadenylation signal"
     rep_origin      complement(5750..6338)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(6509..7300)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="product: β-lactamase gene: bla codon_start: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW* note: confers resistance to ampicillin, carbenicillin, and related antibiotics transl_table: 1"
     CDS             complement(7301..7369)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="product: β-lactamase gene: bla codon_start: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW* note: confers resistance to ampicillin, carbenicillin, and related antibiotics transl_table: 1"
     promoter        complement(7370..7474)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
ORIGIN
        1 gtcgacattg attattgact agagtcatcc tcatcgtcga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 cgcgcgccag gcggggcggg gcgggggcga gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
      721 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      901 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      961 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1021 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
     1081 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     1141 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1201 cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1261 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     1321 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     1381 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     1441 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     1501 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     1561 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     1621 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     1681 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     1741 tcatcatttt ggcaaagaat tcggtaccgc gggcccggga ttacaatgct cgaagtcaag
     1801 gaagcatcac caaccagcat ccagatcagc tggggcaagt acaaggtcat ggttcgctac
     1861 taccgcatca cctacggtga aactggtggc aatagccctg tccaggaatt caccgtgcct
     1921 ggcagcaagt ccactgctac catcagcagc ctgaaacctg gtgtcgacta taccatcacg
     1981 gtgtacgccg tcacgatcga ccactggaac taccaggacc cgatcccgat ctccatcaac
     2041 taccgcaccg gatccgatta caaggatgac gacgataagg gtagcggctc cagtagatct
     2101 gggGCGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     2161 GGCGACATAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
     2221 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     2281 CTCGTGACCA GCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CAACATGAAG
     2341 CGGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
     2401 TTCGAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTTGAGGG CGGCACCCTG
     2461 GTGAACCGCG TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2521 AAGCTGGAGT ACAACTACAA CTGCCACAAC GTCTTTATCA CCGCCGACAG GCAGCAGAAC
     2581 GGCATCAAGG TCAACTTCAA GATCCGCCAC AACATCGAGG TCGGCGGCGT GCAGCTCGCC
     2641 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
     2701 TACCTGAGCT ACCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
     2761 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCAGCA TGGACGAGCT GTACtctagg
     2821 ggagctggcg ctggagcggg tgcaggggct ggctctagat tccagtgccg gatctgcatg
     2881 cggaacttca gcgaccggtc caacctgagc aggcacatca gaacccacac cggagaaaag
     2941 cccttcgcct gcgacatttg cggccggaag ttcgccatca gcagcaacct gaacagccac
     3001 accaagatcc acactggcag ccagaaacct ttccagtgca gaatttgtat gagaaacttt
     3061 agcagaagcg acaacctggc cagacacatc cggacacata ctggtgaaaa accttttgcc
     3121 tgtgatatct gtggcagaaa gtttgccacc tccggcaatc tgacccggca cacaaagatt
     3181 cacctgcggg gcagccagct atcgattgtc gacgctcctg aacaacgtga aggtgcttct
     3241 caagtttctg tttctgttac ttttgaagat gttgctgttc tttttactcg tgatgaatgg
     3301 aaaaaacttg atctttctca acgttctctt tatcgtgaag ttatgcttga aaattattct
     3361 aatcttgctt ctatggctta aacgcgttgt acaagtaaag cggccccgga ctcgaggccg
     3421 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     3481 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     3541 gcctttctct ccacaggtgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3601 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3661 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3721 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3781 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3841 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3901 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3961 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4021 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4081 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4141 gccgcctccc cgcctggaat tcgagctcgg tacgatcagc ctcgactgtg ccttctagtt
     4201 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4261 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4321 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4381 ggccagcttt tgttcccttt agggttaatt gcgcgcttgg cgctaatcat ggtcatagct
     4441 gtttccactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     4501 ctggctcaca aataccactg agatcgtttt ccctctgcca aaaattatgg ggacatcatg
     4561 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     4621 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     4681 catcagaatg agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga
     4741 acaaaggtgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     4801 attccataga aaagccttga cttgaggtta gatttttttt tatattttgt ttgtgttatt
     4861 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     4921 ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatccc tcgacctgca
     4981 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5041 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5101 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5161 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5221 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5281 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5341 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     5401 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5461 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     5521 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5581 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5641 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5701 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5761 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5821 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5881 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5941 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6001 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6061 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6121 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6181 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6241 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6301 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6361 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6421 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6481 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6541 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6601 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6661 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6721 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6781 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6841 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6901 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6961 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7021 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7081 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7141 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7201 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7261 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7321 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7381 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7441 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//