LOCUS       Exported                7915 bp ds-DNA     circular SYN 06-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    NOP FRET Sensor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    235..818
                     /label=CMV promotor
     misc_feature    863..881
                     /label=T7 Promotor
     misc_feature    961..1710
                     /label=NOP
     misc_feature    1708..1710
                     /label=G250
     misc_feature    1711..1716
                     /label=SacII
     misc_feature    1717..2439
                     /label=sYFP2
     misc_feature    2434..2439
                     /label=HpaI
     misc_feature    2440..2742
                     /label=NOP
                     /label=NOP(1)
     misc_feature    2740..2742
                     /label=S351
     misc_feature    2743..2748
                     /label=AgeI
     misc_feature    2749..3462
                     /label=mTur2
     misc_feature    3463..3468
                     /label=EcoRV
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcacc
      961 atggagcccc tgtttccggc ccccttttgg gaagtcatct atggctcaca tctgcagggg
     1021 aacctgagtc tgctgtcccc caaccattca ctgcttcctc cccatctgtt gctgaacgct
     1081 tctcatggag cattcctgcc tctcggattg aaggtaacta tcgtcggctt gtatctcgcc
     1141 gtctgcgtgg gcggcctcct gggtaactgt ctggttatgt acgttattct ccggcacact
     1201 aaaatgaaga cagcaacaaa catttacatc tttaatctgg cactggctga tacactggta
     1261 ttgttgacgc tgcctttcca ggggaccgat attctcctgg gattctggcc tttcgggaac
     1321 gccctgtgta agactgtaat agcaatagat tattacaaca tgtttactag cacattcacg
     1381 ctgacagcca tgagtgtgga tagatacgtg gctatctgcc accccattcg agcactggac
     1441 gtgcgcacct cctcaaaagc ccaagcagtc aatgtggcca tctgggcact ggccagtgtg
     1501 gttggtgtgc ccgtagcgat aatgggcagc gcccaagtgg aggacgaaga gatcgaatgc
     1561 ctcgtggaga ttccaactcc acaggactat tggggccctg tctttgccat ctgtatcttt
     1621 ttgttctctt tcatcgtgcc agtactcgtc atctcagtat gttacagcct gatgattcgc
     1681 aggctccgag gcgttagact gctgagtgga ccgcggatgg tgagcaaggg cgaggagctg
     1741 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1801 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
     1861 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
     1921 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1981 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2041 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2101 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2161 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     2221 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     2281 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg
     2341 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2401 gggatcactc tcggcatgga cgagctgtac aaggttaaca gcagggagaa ggaccgcaac
     2461 ctccgacgca tcacgaggct ggtcctcgtg gttgtggcgg tgttcgtggg ctgttggacc
     2521 cccgtgcagg tcttcgttct cgcccaggga cttggggtgc agccgtcaag cgagacagca
     2581 gtggctatac tgcgcttttg caccgcactg gggtacgtca acagctgcct gaacccaatt
     2641 ctttacgctt ttctcgatga gaatttcaag gcctgcttcc ggaaattctg ttgtgcttca
     2701 gccctgcgac gggatgtaca agtctcagac agagtgaggt caaccggtgt gagcaagggc
     2761 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2821 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2881 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2941 tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     3001 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3061 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3121 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3181 tactttagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     3241 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     3301 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3361 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3421 accgccgccg ggatcactct cggcatggac gagctgtaca aggatatcta gctcgagtct
     3481 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3541 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3601 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3661 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3721 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3781 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3901 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3961 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4021 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4081 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4141 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4201 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4381 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4561 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     4621 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4681 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4741 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4801 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4861 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4921 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4981 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5041 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5101 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     5161 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5221 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5281 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5341 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5401 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5461 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5521 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5581 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5641 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5701 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5761 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5821 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5881 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5941 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6001 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6061 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6601 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
