LOCUS       Exported                7751 bp ds-DNA     circular SYN 06-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    KOR BRET Sensor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     UUID: edacf53f-a5a8-4250-bb98-b752f6d4b1b0
            Type: Synthetic
            ConfirmedExperimentally: 0
            SequenceClass: UNA
            TransformedInto: unspecified
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    235..818
                     /locus_tag="CMV promotor"
                     /label=CMV promotor
                     /label=CMV promotor
     misc_feature    863..881
                     /locus_tag="T7 Promotor"
                     /label=T7 Promotor
                     /label=T7 Promotor
     misc_feature    961..1743
                     /locus_tag="OPRK"
                     /label=OPRK
                     /label=OPRK
     misc_feature    1741..1743
                     /locus_tag="G261"
                     /label=G261
                     /label=G261
     misc_feature    1744..1749
                     /locus_tag="SacII"
                     /label=SacII
                     /label=SacII
     misc_feature    1750..2466
                     /locus_tag="sYFP2"
                     /label=sYFP2
                     /label=sYFP2
     misc_feature    2467..2472
                     /locus_tag="HpaI"
                     /label=HpaI
                     /label=HpaI
     misc_feature    2473..2778
                     /locus_tag="OPRK(1)"
                     /label=OPRK
                     /label=OPRK(1)
                     /label=OPRK(1)
     misc_feature    2776..2778
                     /locus_tag="T363"
                     /label=T363
                     /label=T363
     misc_feature    2779..2784
                     /locus_tag="AgeI"
                     /label=AgeI
                     /label=AgeI
     misc_feature    2785..3298
                     /locus_tag="nLuc"
                     /label=nLuc
                     /label=nLuc
     misc_feature    3299..3304
                     /locus_tag="EcoRV"
                     /label=EcoRV
                     /label=EcoRV
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcacc
      961 atggaatccc cgattcagat cttccgcggg gagccgggcc ctacctgcgc cccgagcgcc
     1021 tgcctgcccc ccaacagcag cgcctggttt cccggctggg ccgagcccga cagcaacggc
     1081 agcgccggct cggaggacgc gcagctggag cccgcgcaca tctccccggc catcccggtc
     1141 atcatcacgg cggtctactc cgtagtgttc gtcgtgggct tggtgggcaa ctcgctggtc
     1201 atgttcgtga tcatccgata cacaaagatg aagacagcaa ccaacattta catatttaac
     1261 ctggctttgg cagatgcttt agttactaca accatgccct ttcagagtac ggtctacttg
     1321 atgaattcct ggccttttgg ggatgtgctg tgcaagatag taatttccat tgattactac
     1381 aacatgttca ccagcatctt caccttgacc atgatgagcg tggaccgcta cattgccgtg
     1441 tgccaccccg tgaaggcttt ggacttccgc acacccttga aggcaaagat catcaatatc
     1501 tgcatctggc tgctgtcgtc atctgttggc atctctgcaa tagtccttgg aggcaccaaa
     1561 gtcagggaag acgtcgatgt cattgagtgc tccttgcagt tcccagatga tgactactcc
     1621 tggtgggacc tcttcatgaa gatctgcgtc ttcatctttg ccttcgtgat ccctgtcctc
     1681 atcatcatcg tctgctacac cctgatgatc ctgcgtctca agagcgtccg gctcctttct
     1741 ggcccgcgga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1801 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1861 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     1921 tggcccaccc tcgtgaccac cctgggctac ggcgtgcagt gcttcgcccg ctaccccgac
     1981 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2041 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2101 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2161 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     2221 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     2281 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2341 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     2401 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2461 tacaaggtta actcccgaga gaaagatcgc aacctgcgta ggatcaccag actggtcctg
     2521 gtggtggtgg cagtcttcgt cgtctgctgg actcccattc acatattcat cctggtggag
     2581 gctctgggga gcacctccca cagcacagct gctctctcca gctattactt ctgcatcgcc
     2641 ttaggctata ccaacagtag cctgaatccc attctctacg cctttcttga tgaaaacttc
     2701 aagcggtgtt tccgggactt ctgctttcca ctgaagatga ggatggagcg gcagagcact
     2761 agcagagtcc gaaatacaac cggtatggtc ttcacactcg aagatttcgt tggggactgg
     2821 cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg
     2881 tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat
     2941 gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg
     3001 ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg
     3061 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
     3121 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
     3181 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
     3241 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtga
     3301 tatctagctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3361 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3421 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3481 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3541 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     3601 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3661 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3721 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3781 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3841 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3901 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3961 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4021 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     4081 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4141 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4201 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4261 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4321 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4381 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     4441 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4501 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4561 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4621 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4681 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4741 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4801 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4861 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4921 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4981 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5041 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5101 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5161 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5221 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5281 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5341 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5401 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5461 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5521 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5581 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5641 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5701 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6421 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt
     6541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6661 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6721 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6781 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6841 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6901 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6961 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7021 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7081 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7141 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7201 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7261 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7321 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7381 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7441 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7501 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7561 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7621 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7681 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7741 cacctgacgt c
//
