LOCUS       Exported                7203 bp ds-DNA     circular SYN 06-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3/OPRM_nLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     UUID: de5ee73f-6172-4c0e-a9fa-de27519f60a6
            Type: Synthetic
            ConfirmedExperimentally: 0
            SequenceClass: UNA
            TransformedInto: unspecified
            Comments: 4
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..809
                     /locus_tag="CMV\promoter(1)"
                     /label=CMV\promoter
                     /label=CMV\promoter(1)
                     /note="/vntifkey=29"
     misc_feature    745..762
                     /locus_tag="pEGFPN1for"
                     /label=pEGFPN1for
     misc_feature    769..789
                     /locus_tag="CMV for"
                     /label=CMV for
     promoter        810..863
                     /locus_tag="CMV\promoter"
                     /label=CMV\promoter
                     /note="/vntifkey=29"
     misc_feature    863..882
                     /locus_tag="T7"
                     /label=T7
     promoter        864..882
                     /locus_tag="T7\promoter"
                     /label=T7\promoter
                     /note="/vntifkey=30"
     misc_feature    889..894
                     /locus_tag="Polylinker"
                     /label=Polylinker
     misc_feature    889..894
                     /locus_tag="HindIII"
                     /label=HindIII
     CDS             964..990
                     /codon_start=1
                     /locus_tag="HA-Tag"
                     /label=HA-Tag
                     /translation="YPYDVPDYA"
     misc_feature    1003..2193
                     /locus_tag="MOR rattus norvegicus"
                     /label=MOR rattus norvegicus
     misc_feature    2194..2199
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    2200..2713
                     /locus_tag="nLuc"
                     /label=nLuc
     misc_feature    2716..2721
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    2756..2774
                     /locus_tag="SP6"
                     /label=SP6
     promoter        complement(2756..2773)
                     /locus_tag="SP6\promoter"
                     /label=SP6\promoter
                     /note="/vntifkey=30"
     polyA_signal    2775..3006
                     /locus_tag="BGH\poly\A"
                     /label=BGH\poly\A
                     /note="/vntifkey=25"
     misc_feature    2793..2810
                     /locus_tag="pCR3.1-BGHrev"
                     /label=pCR3.1-BGHrev
     misc_feature    2797..2816
                     /locus_tag="pcDNA3 rev"
                     /label=pcDNA3 rev
     misc_feature    3506..3526
                     /locus_tag="pcDNA3_for Primer"
                     /label=pcDNA3_for Primer
     misc_feature    3506..3526
                     /locus_tag="pBABE3"
                     /label=pBABE3
     misc_feature    3506..3526
                     /locus_tag="pBabe3 Primer, rev"
                     /label=pBabe3 Primer, rev
     promoter        3547..3872
                     /locus_tag="SV40\promoter"
                     /label=SV40\promoter
                     /note="/vntifkey=30"
     rep_origin      3741..3826
                     /direction=BOTH
                     /locus_tag="SV40"
                     /label=SV40
                     /note="origin of replication"
     misc_feature    3908..4702
                     /locus_tag="Neomycin"
                     /label=Neomycin
                     /note="/vntifkey=21"
     polyA_signal    4757..5129
                     /locus_tag="SV40\poly\A"
                     /label=SV40\poly\A
                     /note="/vntifkey=25"
     misc_feature    4967..4986
                     /locus_tag="pCEP reverse"
                     /label=pCEP reverse
     misc_feature    5045..5062
                     /locus_tag="M13 rev (-29)"
                     /label=M13 rev (-29)
     misc_feature    5059..5082
                     /locus_tag="M13 rev (-49)"
                     /label=M13 rev (-49)
     misc_feature    5104..5129
                     /locus_tag="pEX-Rev"
                     /label=pEX-Rev
     rep_origin      5389..6062
                     /direction=BOTH
                     /locus_tag="ColE1\origin"
                     /label=ColE1\origin
                     /note="/vntifkey=33"
     misc_feature    complement(6207..7067)
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /note="/vntifkey=21"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcggcttgc cttcagcacc
      961 atgtacccat acgatgtgcc cgactatgct ctggtgccgc gggacagcag caccggccca
     1021 gggaacacca gcgactgctc agacccctta gctcaggcaa gttgctcccc agcacctggc
     1081 tcctggctca acttgtccca cgttgatggc aaccagtccg atccatgcgg tctgaaccgc
     1141 accgggcttg gcgggaacga cagcctgtgc cctcagaccg gcagcccttc catggtcaca
     1201 gccattacca tcatggccct ctactctatc gtgtgtgtag tgggcctctt cggaaacttc
     1261 ctggtcatgt atgtgattgt aagatacacc aaaatgaaga ctgccaccaa catctacatt
     1321 ttcaaccttg ctctggcaga cgccttagcg accagtacac tgccctttca gagtgtcaac
     1381 tacctgatgg gaacatggcc cttcggaacc atcctctgca agatcgtgat ctcaatagat
     1441 tactacaaca tgttcaccag catattcacc ctctgcacca tgagcgtgga ccgctacatt
     1501 gctgtctgcc acccagtcaa agccctggat ttccgtaccc cccgaaatgc caaaatcgtc
     1561 aacgtctgca actggatcct ctcttctgcc atcggtctgc ctgtaatgtt catggcaacc
     1621 acaaaataca ggcaggggtc catagattgc accctcacgt tctcccaccc aacctggtac
     1681 tgggagaacc tgctcaaaat ctgtgtcttt atcttcgctt tcatcatgcc gatcctcatc
     1741 atcactgtgt gttacggcct gatgatctta cgactcaaga gcgttcgcat gctatcgggc
     1801 tccaaagaaa aggacaggaa tctgcgcagg atcacccgga tggtgctggt ggtcgtggct
     1861 gtatttatcg tctgctggac ccccatccac atctacgtca tcatcaaagc gctgatcacg
     1921 attccagaaa ccacatttca gaccgtttcc tggcacttct gcattgcttt gggttacacg
     1981 aacagctgcc tgaatccagt tctttacgcc ttcctggatg aaaacttcaa gcgatgcttc
     2041 agagagttct gcatcccaac ctcgtccacg atcgaacagc aaaactccac tcgagtccgt
     2101 cagaacacta gggaacatcc ctccacggct aatacagtgg atcgaactaa ccaccagcta
     2161 gaaaatctgg aggcagaaac tgctccattg cccaccggta tggtcttcac actcgaagat
     2221 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
     2281 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
     2341 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
     2401 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
     2461 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
     2521 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
     2581 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
     2641 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
     2701 cgcattctgg cgtaatctag ataactgggt ctcacaccat ctagagggcc ctattctata
     2761 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     2821 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2881 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2941 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3001 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3061 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
     3241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3421 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
     3481 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3541 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3601 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3661 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3721 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3781 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3841 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     3901 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3961 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4021 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4081 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4141 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4201 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4261 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4321 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4381 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4441 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4501 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4561 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4621 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4681 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4741 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4801 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4861 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4981 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5041 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5101 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5161 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5221 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5341 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5401 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5461 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5521 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5581 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5641 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5701 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5761 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5821 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5881 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5941 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6001 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6061 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6121 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6181 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6241 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6301 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6361 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6421 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6481 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6541 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6601 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6661 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6721 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6781 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6841 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6901 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6961 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7021 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7081 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7141 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7201 gtc
//
