LOCUS       AAV-DIO-SPOTLigh        7076 bp DNA     circular SYN 06-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   VB220426-1051wsa
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     Sequence Label: AAV-DIO-SPOTLight-U2GCR
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="synthetic DNA construct"
     protein_bind    137..157
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        158..955
                     /label=CBh
                     /note="/application_notes=Strong promoter."
                     /note="/description=CMV early enhancer fused to modified 
                     chickenbeta-actin promoter"
                     /note="/full_name=The short version of CAG promoter"
                     /note="/official_designation=CBh"
                     /note="/uuid=ebd6140b3e0445c4a59db5b875853bca"
     protein_bind    980..1013
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     misc_feature    1046..1079
                     /label=LoxP
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/description=Locus of X(cross)-over in P1"
                     /note="/official_designation=LoxP"
                     /note="/uuid=b48e25a55648467f8c98b3f68faf613f"
     misc_feature    complement(1092..3952)
                     /label=SPOTLight U2GCR
                     /label={SPLT 1.0}
                     /label=nonstandard type:  ORF
                     /note="/application_notes="
                     /note="/official_designation=SPOTLight U2GCR"
                     /note="/uuid=a97376394505431c93011792c6b658c9"
     regulatory      3546..3555
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3959..3992)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    complement(4025..4058)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(4059..4083)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4113..4320
                     /label=BGH pA
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
     repeat_region   4328..4468
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4543..4998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5280..5384
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5385..6245
                     /label=AmpiciIIin
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
     rep_origin      6416..7004
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
     repeat_region   join(7066..7076,1..130)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
      241 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      301 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      361 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      421 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      481 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      541 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      601 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      661 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      721 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagctg agcaagaggt aagggtttaa gggatggttg gttggtgggg
      901 tattaatgtt taattacctg gagcacctgc ctgaaatcac tttttttcag gttggcaagt
      961 ttgtacaaaa aagcaggcta taacttcgta taggatactt tatacgaagt tatcctcgag
     1021 taccggtcac gcgtacgcaa gcttgataac ttcgtatagc atacattata cgaagttatc
     1081 ctagggccac cttacttgta cagctcgtcc atgccgtaca ggaacaggtg gtggcggccc
     1141 tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
     1201 ttggtgtcca cgtagtagta gccgggcagt tgcacgggct tcttggccat gtagatggtc
     1261 ttgaactcca ccaggtagcg gccgccgtcc ttcagcttca gggcctggtg gatctcgccc
     1321 ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc catggtcttc
     1381 ttctgcatta cggggccgtc gggggggaag tttgtgccgc gcatcttcac cttgtagatc
     1441 agcgtgccgt cctgcaggga ggagtcctgg gtcacggtca ccagaccgcc gtcctcgaag
     1501 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta atcggggatg
     1561 tcggcggggt gcttcacgta cgccttggag ccgtacatga actgggggga caggatgtcc
     1621 caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg ggtgccctcg
     1681 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcat ggagccctcc
     1741 atgcgcacct tgaagcgcat gaactctttg atgacggcca tgttgttgtc ctcggaggag
     1801 gcggtgccgg agctgccact accagtacta ccagtaccgt gtcctaggaa caagtgatgg
     1861 cgtccctctg atcgctcgta ttgttcaacg atagtgtagt cttcattatg ggatgttatg
     1921 tccaacttag tgtcgacgta atagtaacca ggcaactgca caggcttctt agccatatag
     1981 atagttttaa attcaacaag gtaatggccg ccgtccttga gtttaagagc ctggtggatc
     2041 tcgcctttga ggacaccatc cctaggataa agtcgctcag tgctggcctc ccagcccatt
     2101 gtcttctttt gcataacggg accgtcgggg gggaagttgg tacctctcat cttaactttg
     2161 taaatcaatg taccgtcttg aaggctagag tcttgggtta ctgttacgag ccccccgtct
     2221 tcaaaattca tcactcgctc ccatttgaac ccttctggga aggaaagttt cttataatct
     2281 ggaatatcgg cgggatgttt gacatatgcc ttactgccgt acataaactg aggactgagg
     2341 atgtcccatg caaatggcaa aggtccgcct tttgtgactt tcaatttagc ggtctgagtg
     2401 ccctcgtaag gtcgtccctc tccctcccct tcgatttcaa attcgtggcc gttcatagat
     2461 ccctccattc gtaccttgaa ccgcataaac tccttaatca cctcctcgcc cttgctaacc
     2521 accagatcct cttctgagat gagtttttgt tcagtcatgt tgtggggctt tgctggattc
     2581 gaggaatgtg cttaactcga aggtatcttt gtccgttaca gcaacactgc tgctggattt
     2641 cgtgaagagc gccacaggtc cagggttctc ctccacgtct ccagcctgct tcagcaggct
     2701 gaagttagta gctccgcttc ctccactacc cttgtacagc tcgtccatgc cgagagtgat
     2761 cccggcggcg gtcacgaact ccagcaggac catatgatcg cgcttctcgt tggggtcttt
     2821 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     2881 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     2941 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatta tatagacgtt
     3001 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     3061 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     3121 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     3181 catcgcactc ttgaagaagt cgtgctgctt catatgatcg gggtagcggc tgaagcactg
     3241 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     3301 gcagatgaac ttcagggtca gcttgccgta ggtggcgtcg ccctcgccct cgccggacac
     3361 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     3421 gaacagctcc tcgcccttgc tcaccgccat ggcttaggcc ggtgggggtt gccgctgcag
     3481 agcctggtgc tgctgccgcc gctgcaaagg ccaacgctgc cgcaggcact gctgcctcta
     3541 atacgccatg gtggccgcaa gccctgcggg aggggaagag gaaagggaca cccgagcaca
     3601 caacttaaac cggcagacag caagcagaaa tggcggcggc acgccctaaa cccgcccttt
     3661 atagcctcgg ccagggacgc agcacaccgc gtatcctccc actgtggtca cgtgatccta
     3721 ccgcagcgcc accaaaccgc ctctcactga cgccctcgcc cgccccaacc cttctccgct
     3781 gtgctcgggg ggtaactgtg gcgttagaga tcgtcctaaa ggccccatga gagaaaagtg
     3841 cactacttta taggttgacg accagccgcg gtctggttcc ggagcgcgga gggatcgctc
     3901 gttgacgaat ttccggtgtc ttacaaggcg gggtgggaca cctagccgga tctctagaat
     3961 aacttcgtat aaagtatcct atacgaagtt ataggtacct gagctcaaca tcgatagcac
     4021 tagtataact tcgtataatg tatgctatac gaagttatac ccagctttct tgtacaaagt
     4081 gggaattcct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4141 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4201 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4261 tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc atgctgggga
     4321 gggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4381 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4441 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4501 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4561 cattaagcgc ggcgggggtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     4621 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4681 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4741 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4801 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4861 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4921 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4981 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5041 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5101 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5161 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5221 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag
     6121 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6181 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6241 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6541 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcag
//
