LOCUS       HaloTag-LaminA_GK074    6890 bp ds-DNA     circular     09-DEC-2024
DEFINITION  GK074.
ACCESSION   GK074
VERSION     GK074
COMMENT     Imported using the Genbank importer. File name: 457CFL_5_GK074.gbk
            Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             597..3548
                     /label="HaloTag-LaminA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGRSRAQASNSAVDGTAGSAANLPAMETPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEKEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPAEYNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRSVGGSGGGSFGDNLVTRSYLLGNSSPRTQSPQNCSIM*"
     CDS             600..1484
                     /label="HaloTag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1554..3545
                     /label="LaminA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     polyA_signal    3678..3799
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      complement(3833..4261)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        4288..4392
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4394..4751
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4786..5580
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             5601..5753
                     /label="ble (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHP"
     polyA_signal    5812..5859
                     /label="HSV TK poly(A) signal"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(5950..5966)
                     /label="bGH poly(A) signal (fragment)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="polyA_signal"
     rep_origin      6188..6776
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc gagcgcatgc
      661 actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac ggtaacccga
      721 cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat cgctgcattg
      781 ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat ttcttcgacg
      841 accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag gtcgtcctgg
      901 tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat ccagagcgcg
      961 tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac gaatggccag
     1021 aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc aagctgatca
     1081 tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg
     1141 aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt
     1201 ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg
     1261 aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc tggggcaccc
     1321 caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg cctaactgca
     1381 aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg gacctgatcg
     1441 gcagcgagat cgcgcgctgg ctgtccacgc tcgagatttc cggcagatct cgagctcaag
     1501 cttcgaattc tgcagtcgac ggtaccgcgg gcagcgctgc caacctgccg gccatggaga
     1561 ccccgtccca gcggcgcgcc acccgcagcg gggcgcaggc cagctccact ccgctgtcgc
     1621 ccacccgcat cacccggctg caggagaagg aggacctgca ggagctcaat gatcgcttgg
     1681 cggtctacat cgaccgtgtg cgctcgctgg aaacggagaa cgcagggctg cgccttcgca
     1741 tcaccgagtc tgaagaggtg gtcagccgcg aggtgtccgg catcaaggcc gcctacgagg
     1801 ccgagctcgg ggatgcccgc aagacccttg actcagtagc caaggagcgc gcccgcctgc
     1861 agctggagct gagcaaagtg cgtgaggagt ttaaggagct gaaagcgcgc aataccaaga
     1921 aggagggtga cctgatagct gctcaggctc ggctgaagga cctggaggct ctgctgaact
     1981 ccaaggaggc cgcactgagc actgctctca gtgagaagcg cacgctggag ggcgagctgc
     2041 atgatctgcg gggccaggtg gccaagcttg aggcagccct aggtgaggcc aagaagcaac
     2101 ttcaggatga gatgctgcgg cgggtggatg ctgagaacag gctgcagacc atgaaggagg
     2161 aactggactt ccagaagaac atctacagtg aggagctgcg tgagaccaag cgccgtcatg
     2221 agacccgact ggtggagatt gacaatggga agcagcgtga gtttgagagc cggctggcgg
     2281 atgcgctgca ggaactgcgg gcccagcatg aggaccaggt ggagcagtat aagaaggagc
     2341 tggagaagac ttattctgcc aagctggaca atgccaggca gtctgctgag aggaacagca
     2401 acctggtggg ggctgcccac gaggagctgc agcagtcgcg catccgcatc gacagcctct
     2461 ctgcccagct cagccagctc cagaagcagc tggcagccaa ggaggcgaag cttcgagacc
     2521 tggaggactc actggcccgt gagcgggaca ccagccggcg gctgctggcg gaaaaggagc
     2581 gggagatggc cgagatgcgg gcaaggatgc agcagcagct ggacgagtac caggagcttc
     2641 tggacatcaa gctggccctg gacatggaga tccacgccta ccgcaagctc ttggagggcg
     2701 aggaggagag gctacgcctg tcccccagcc ctacctcgca gcgcagccgt ggccgtgctt
     2761 cctctcactc atcccagaca cagggtgggg gcagcgtcac caaaaagcgc aaactggagt
     2821 ccactgagag ccgcagcagc ttctcacagc acgcacgcac tagcgggcgc gtggccgtgg
     2881 aggaggtgga tgaggagggc aagtttgtcc ggctgcgcaa caagtccaat gaggaccagt
     2941 ccatgggcaa ttggcagatc aagcgccaga atggagatga tcccttgctg acttaccggt
     3001 tcccaccaaa gttcaccctg aaggctgggc aggtggtgac gatctgggct gcaggagctg
     3061 gggccaccca cagcccccct accgacctgg tgtggaaggc acagaacacc tggggctgcg
     3121 ggaacagcct gcgtacggct ctcatcaact ccactgggga agaagtggcc atgcgcaagc
     3181 tggtgcgctc agtgactgtg gttgaggacg acgaggatga ggatggagat gacctgctcc
     3241 atcaccacca cggctcccac tgcagcagct cgggggaccc cgctgagtac aacctgcgct
     3301 cgcgcaccgt gctgtgcggg acctgcgggc agcctgccga caaggcatct gccagcggct
     3361 caggagccca ggtgggcgga cccatctcct ctggctcttc tgcctccagt gtcacggtca
     3421 ctcgcagcta ccgcagtgtg gggggcagtg ggggtggcag cttcggggac aatctggtca
     3481 cccgctccta cctcctgggc aactccagcc cccgaaccca gagcccccag aactgcagca
     3541 tcatgtaagg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     3601 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3661 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3721 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3781 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     3841 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     3901 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     3961 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4021 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     4081 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     4141 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     4201 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     4261 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4381 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     4441 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4501 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4561 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4621 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4681 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4741 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4801 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4861 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4921 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     4981 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5041 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5101 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5161 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5221 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5281 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     5341 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5401 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5461 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5521 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5581 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5641 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5701 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     5761 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     5821 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     5881 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     5941 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     6001 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     6061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6301 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//