LOCUS       pcDNA4-mCherry-cGAS-3xH 7332 bp ds-DNA     circular     09-DEC-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(182..770)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(944..1603)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2172..2551
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2552..2751
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2921..5239
                     /label="mCherry-cGAS-3xHA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKLEMQPWHGKAMQRASEAGATAPKASARNARGAPMDPTESPAAPEAALPKAGKFGPARKSGSRQKKSAPDTQERPPVRATGARAKKAPQRAQDTQPSDATSAPGAEGLEPPAAREPALSRAGSCRQRGARCSTKPRPPPGPWDVPSPGLPVSAPILVRRDAAPGASKLRAVLEKLKLSRDDISTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLEVPRIQLEEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASKMLSKFRKIIKEEINDIKDTDVIMKRKRGGSPAVTLLISEKISVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKLMKYLLEQLKERFKDKKHLDKFSSYHVKTAFFHVCTQNPQDSQWDRKDLGLCFDNCVTYFLQCLRTEKLENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEFSGRYPYDVPDYA*"
     Fluorophore     2924..3627
                     /label="mCherry"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3635..5200)
                     /label="cGAS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5210..5236
                     /label="3x HA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     polyA_signal    5283..5507
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    complement(6942..7063)
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
     2761 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
     2821 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     2881 ccgatccagc ctccggactc tagcgtttaa actaaagctt atggtgagca agggcgagga
     2941 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     3001 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3061 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     3121 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     3181 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3241 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3301 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     3361 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     3421 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     3481 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     3541 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     3601 caccggcggc atggacgagc tgtacaagct cgagatgcag ccttggcacg gaaaggccat
     3661 gcagagagct tccgaggccg gagccactgc ccccaaggct tccgcacgga atgccagggg
     3721 cgccccgatg gatcccaccg agtctccggc tgcccccgag gccgccctgc ctaaggcggg
     3781 aaagttcggc cccgccagga agtcgggatc ccggcagaaa aagagcgccc cggacaccca
     3841 ggagaggccg cccgtccgcg caactggggc ccgcgccaaa aaggcccctc agcgcgccca
     3901 ggacacgcag ccgtctgacg ccaccagcgc ccctggggca gaggggctgg agcctcctgc
     3961 ggctcgggag ccggctcttt ccagggctgg ttcttgccgc cagaggggcg cgcgctgctc
     4021 cacgaagcca agacctccgc ccgggccctg ggacgtgccc agccccggcc tgccggtctc
     4081 ggcccccatt ctcgtacgga gggatgcggc gcctggggcc tcgaagctcc gggcggtttt
     4141 ggagaagttg aagctcagcc gcgatgatat ctccacggcg gcggggatgg tgaaaggggt
     4201 tgtggaccac ctgctgctca gactgaagtg cgactccgcg ttcagaggcg tcgggctgct
     4261 gaacaccggg agctactatg agcacgtgaa gatttctgca cctaatgaat ttgatgtcat
     4321 gtttaaactg gaagtcccca gaattcaact agaagaatat tccaacactc gtgcatatta
     4381 ctttgtgaaa tttaaaagaa atccgaaaga aaatcctctg agtcagtttt tagaaggtga
     4441 aatattatca gcttctaaga tgctgtcaaa gtttaggaaa atcattaagg aagaaattaa
     4501 cgacattaaa gatacagatg tcatcatgaa gaggaaaaga ggagggagcc ctgctgtaac
     4561 acttcttatt agtgaaaaaa tatctgtgga tataaccctg gctttggaat caaaaagtag
     4621 ctggcctgct agcacccaag aaggcctgcg cattcaaaac tggctttcag caaaagttag
     4681 gaagcaacta cgactaaagc cattttacct tgtacccaag catgcaaagg aaggaaatgg
     4741 tttccaagaa gaaacatggc ggctatcctt ctctcacatc gaaaaggaaa ttttgaacaa
     4801 tcatggaaaa tctaaaacgt gctgtgaaaa caaagaagag aaatgttgca ggaaagattg
     4861 tttaaaacta atgaaatacc ttttagaaca gctgaaagaa aggtttaaag acaaaaaaca
     4921 tctggataaa ttctcttctt atcatgtgaa aactgccttc tttcacgtat gtacccagaa
     4981 ccctcaagac agtcagtggg accgcaaaga cctgggcctc tgctttgata actgcgtgac
     5041 atactttctt cagtgcctca ggacagaaaa acttgagaat tattttattc ctgaattcaa
     5101 tctattctct agcaacttaa ttgacaaaag aagtaaagaa tttctgacaa agcaaattga
     5161 atatgaaaga aacaatgagt ttccagtttt tgatgaattt agcggccgct acccatacga
     5221 tgttccagat tacgcttgac tcgagtctag agggcccgtt taaacccgct gatcagcctc
     5281 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5341 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5401 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5461 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5521 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5581 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5641 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5701 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5761 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5821 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5881 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5941 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6241 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6301 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6361 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     6421 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     6481 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     6541 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     6601 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     6661 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     6721 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     6781 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     6841 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6901 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7081 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7321 gtattgggcg ct
//