LOCUS       Exported               15415 bp ds-DNA     circular SYN 21-MAR-2023
DEFINITION  Neelamegham_X3D_2_LVDP_CMV-NME_EF1a-LucPS9     coverage: 171x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2344..2363
                     /label=NME1stSeq1
     misc_feature    2384..2593
                     /label=sMAR8
     primer_bind     2640..2685
                     /label=LVDP NME insert Rev
     misc_feature    2666..3254
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2772..2783
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(3270..3500)
                     /codon_start=1
                     /label=E protein
                     /translation="MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCN
                     IVNVSLVKPSFYVYSRVKNLNSSRVPDLLVL"
     misc_feature    3497..3506
                     /label=Kozak
     misc_feature    3538..4111
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(4120..4788)
                     /codon_start=1
                     /label=M protein
                     /translation="MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRF
                     LYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFA
                     RTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKD
                     LPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ
                     "
     CDS             complement(4789..4842)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    complement(4855..6108)
                     /label=N protein
     primer_bind     5882..5901
                     /label=NME1stSeq2
     primer_bind     complement(6088..6138)
                     /label=LVDP NME insert Fwd
     misc_feature    6106..6114
                     /label=Kozak
     promoter        complement(6145..6348)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6349..6652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(6723..7170)
                     /label=cHS4
     primer_bind     7161..7182
                     /label=LVDP lucPS9 insert PacI Fwd
     misc_feature    complement(7190..7550)
                     /label=Tactb
     primer_bind     7535..7554
                     /label=LVDPAsisISeqFwd
     misc_feature    complement(7579..7633)
                     /label='spa
     primer_bind     complement(7608..7648)
                     /label=LVDP lucPS9 insert PacI Rev
     primer_bind     7631..7677
                     /label=LVDP Luc-PS9 insert Rev (no WPRE)
     misc_feature    7661..8752
                     /label=SARS-CoV-2 PS9
     primer_bind     complement(7881..7900)
                     /label=LVDPAsisISeqRev
     CDS             complement(8798..10450)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     9296..9321
                     /label=LVDP 1stAgeI Fwd
     primer_bind     10304..10323
                     /label=LVDPAgeISeqFwd
     primer_bind     complement(10441..10465)
                     /label=LVDP Luc-PS9 insert  Fwd
     primer_bind     complement(10446..10485)
                     /label=LVDP 2ndAgeI Luc Rev
     regulatory      10447..10456
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(10466..10677)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     10466..10485
                     /label=EF1a Rev
     primer_bind     10660..10699
                     /label=LVDP PmeI Fwd
     primer_bind     complement(10660..10679)
                     /label=EF1a Fwd
     LTR             11217..11397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11404..11427)
                     
     polyA_signal    11429..11653
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11699..12127
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        12141..12470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12321..12456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        12518..12565
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12584..12958
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13088..13209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13258..13274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13282..13298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13306..13336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13351..13372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13660..14248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14419..15279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15280..15384)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcatc tttaatttct
     2401 aatatattta gaatctttaa tttctaatat atttagaatc tttaatttct aatatattta
     2461 gaatctttaa tttctaatat atttagaatc tttaatttct aatatattta gaatctttaa
     2521 tttctaatat atttagaatc tttaatttct aatatattta gaatctttaa tttctaatat
     2581 atttagaagg atctccgcgt ttatgaacaa acgacccaac acccgtgggt tttttctgtc
     2641 tttttattgc cgtcattctc gaggggcggg gaggcggccc aaagggagat ccgactcgtc
     2701 tgagggcgaa ggcgaagatg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
     2761 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
     2821 gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa caccacggaa
     2881 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
     2941 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
     3001 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
     3061 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
     3121 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
     3181 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
     3241 tccagaggtt gattgtcgag tcggtcgagt taaagaacga ggagatccgg caccctgcta
     3301 gaattgagat tcttcaccct gctgtaaacg tagaatgagg gtttcacgag agatacattg
     3361 acaatgttac aacagtaggc gcaaagcctc aatgcagtca gaattgccag tgtgacgagg
     3421 agaaatacga cgaatgccag aaacaagagc acagaattta cgatcagtgt cccggtttct
     3481 tctgatacga agctgtacat ggtggcggcg aatttctcct tgtgggttgt ggcaagctta
     3541 tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt
     3601 taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca
     3661 atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt
     3721 gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg
     3781 tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg
     3841 ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc
     3901 ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg
     3961 aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa
     4021 tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc
     4081 agtaacgtta gggggggggg agggagaggg gcgggatcct tactggacga gcaaagcaat
     4141 gttatcagaa gatgatgagt ggtctgtatt cagtttatag ttccctatcc tgtagcgtga
     4201 ataggctgca aatccactat cgccagcgac cctttgggac gctccaagct tgtaatagct
     4261 caaagttcga ctggtagcta cagttatttc ttttggcaaa tccttaatat cacagcgacc
     4321 aagatgatgt cctgcgatcc tcaagtggcc cctcaggatc acggctccaa tcaccaattc
     4381 tgattcaagg agcggtcggg tcaaaattgt gccatgcagg gggacattaa gcaagatatt
     4441 tgtttcaggg ttaaatgacc acatgcttcg cgtgcgtgca aaaagtctga atgacgctat
     4501 aaaataggag agccacatca aacctaccag gcatgccata gctattgcta tgccgccagt
     4561 tatccagttt attcgataca ccgccgcgag tacgaagcag gcaagggtca caggccacag
     4621 gagccaaagg aatataagtt ttataatata gaggaatcgg ttgcgattcg cgtatgcgaa
     4681 ttgcaggaga caaatccatg tcaagaagag aaatccaatc acaaggttcc attgctccaa
     4741 cagttttttc agctcctcta cagtaatggt cccatttgag tcggccattg ggccaggatt
     4801 ctcctccacg tcaccgcatg ttaggagact tcctctgccc tctcctccgg acccctgggt
     4861 gctatcggcg ctagacatag actgctggag ctgcttggaa aaatcgtcca ggtcggcggc
     4921 tggcagcagt gtcacggtct gctgcttctt ctgtctctga ggcagggcct gggtctcatc
     4981 ggccttcttc ttcttgtcct tcttgggctc tgtaggggga aaggtcttat aggcatcgat
     5041 gtgcttgttc agcaggatga cctgatcctt gaagttaggg tccttatcgt ccagcttgat
     5101 ggcgcctgtg taggtcagcc atgtgccgga tggggtcacc tccattccga tcctgctcat
     5161 gccaaagaag gcggaggcgc ttggtgcgaa ctgtgcgatc tgaggccagt gcttataatc
     5221 ggtgccctgc ctgatcagct cctggtcgcc aaaattgccc tgtgtctgct ctggtccgcg
     5281 cctgccgaag gcctgggtca cgttgtaggc ctttgtggcg gtgcgcttct gccttggctt
     5341 cttgctggcc tcggcggcag acttctttgt cacggtctgt ccctgctgct gctgtccctt
     5401 gccgctcatc ttagactcca gctgattcag tctatccagc agcagcaggg ccagggcggc
     5461 gtcgcctccg tttcctgcca ttcttgcagg gcttgtgccc atggagctgc ctggggtaga
     5521 attccgagag gagtttctgg accggctaga ggagcggcta gaggcctggg agccgcccct
     5581 gctgccctct gcgtaaaagc ccttgggcag tgtggtgccc tgtggcagct ggagcacgat
     5641 tgctgcattg ttggcgggat tgcgtgtgcc gatgtgatcc tttggtgtgt tcagggcgcc
     5701 ctcggttgcc acccagatga ttccgtcctt attggcgcca tatggcagtc ctgcctcagg
     5761 tccggtgccc agatagtaga agtaccatct gggggacaga tccttcatct tgccgtcgcc
     5821 tccccggatt ctccttgttg ccctcctata gtagccgatc tgatcgtcag gggagctatt
     5881 ggtgttgata ggcacgccct ggcccctggg aaacttcagg tcctccttgc cgtgctgtgt
     5941 cagggcggtg aaccagctgg cggtattgtt gggcaggccc tgtggtctcc gctgcttaga
     6001 gcgtgctccg gacctctctc cattctggtt ggagcctgta gaatcggagg ggccgccgaa
     6061 ggtgattctt ggggcattcc gctggttctg agggccgttg tcagacatgg tggcgagcta
     6121 gcggatctga cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct
     6181 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     6241 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     6301 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     6361 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     6421 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     6481 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     6541 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     6601 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     6661 catatatggg ctatgaacta atgaccccgt aattgattac tattaatcga atttgtggat
     6721 ccgtgacgca ctgaacaggt tgcccaagga ggctgtggat gccccatccc tgcaggcatt
     6781 caaggccagg ctggatgtgg ctctgggcag cctgggctgc tggttggcga ccctgcacat
     6841 agcagggggt tggagctggg tgagcactgt gctcctttgc aacccaggcc attctatgat
     6901 tctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc
     6961 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcgcgtgc caccttcccc
     7021 gtgccgggct gtccccgcac gctgccggct ccggctcggg gatgcggggg gagcgccgga
     7081 ccggagcgga gccccgggcg gctcgctgct gccccctagc ggaagaggga tgcaattaca
     7141 tccctggggg ctttggcggg gggctctccc ttaattaact gacagccacg atcccatagg
     7201 tgaaggcaaa ggcgtggcag ctaggaacac tcactacacc cccactcaag ggaacaccgt
     7261 gcccagctac cctgagacag ccccactccc aggaaatgca ggtgccatgc ctcactgggg
     7321 ctggttggca cctgagccag acacccccaa atagtccctt cccacctcct caagcatctg
     7381 cactctgggt gaacccagaa aaactggggt acctggggag acatagaagg gccaggccag
     7441 gaactcccca ataagcagga acagagacct gacccctgag cctcccccac cctctaaggc
     7501 tgctcaatgt caaggcagga gggatctcca cacacctgca ctctgggtaa ggacaagttg
     7561 gcccccactg ccccacctct agacacacaa aaaaccaaca cacagatcta atgaaaataa
     7621 agatctttta tttctagcag gcgatcgcgg agttgggatc tatctttata gccacggaac
     7681 ctccaagagc tagcttttgt tgtataaacc cacaaatgta agtgaaaaaa ccctctttag
     7741 agtcattttc ttttgtaaca tttttagtct tagggtcgta catatcacta ataatgagat
     7801 cccatttatt agctgtatgt acagttgcac aatcaccaat caaagttgaa tctgcatcag
     7861 agacaaagtc attaagatct gaatcgacaa gcagcgtacc cgtaggcaac cactgtctta
     7921 aaacagctgt acctggtgca actcctttat cagaaccagc accaaaatgt ataactctca
     7981 tattataggg tacagctaat gttaatgtgt ttaaatattg acacagttga gtatattttg
     8041 cgacattcat cattatgcct ttaggtaatg ttgcactatc accataattt tgaaggtcac
     8101 acttttctaa tagcattctt tgcattttgt aaagattagg catagcaaca cccggttgcc
     8161 acgcttgact agattgtaat tttgggtaaa atgtttctac atggccatct ttacaccaaa
     8221 gcataaatga aatttctgta tagtcaatag tcactttgac aaccttagaa actacagata
     8281 aatcttggga ttttattatt tcaacaaaat catcaagtaa taaatcaata acagaacaca
     8341 cacacttaga tgaacctgtt tgcgcatctg ttatgaaata gtttttaact gtactgtcca
     8401 taggaataaa atcttctaat tcaaaaggtg attccttaaa acgtttagct agtccaatca
     8461 gtagatgtaa accacctaac tgactatgac taaaatctcc ataaacgata tgttcgaagg
     8521 catagccttc taatttatac cgttcaatga attcatccat agctaattct aagaaatcaa
     8581 tttccatttg actcctgggt ttaaattctt gtaaatttct actctgagta aagtaagttt
     8641 caggtaattg ttggacaaca ccatcaactt tcttataata attgaactgt gtttttacgg
     8701 cttctccaat taatgtgact ccattaagac tagcttgttt gggacctaca gagatctata
     8761 tctccttctt aaaggaggcg gccgcataag cgaattctta cacggcgatc ttgccgccct
     8821 tcttggcctt aatgagaatc tcgcggatct tgcgggcgtc caacttgccg gtcagtcctt
     8881 taggcacctc gtccacgaac acaacaccac cgcgcagctt cttggcggtt gtaacctggc
     8941 tggccacata gtccacgatc tccttctcgg tcatggtttt accgtgttcc agcacgacga
     9001 ctgcggcggg cagctcgccg gcatcgtcgt cgggcaggcc ggcgaccccg gcgtcgaaga
     9061 tgttggggtg ttgcagcagg atgctctcca gttcggctgg ggctacctgg tagcccttgt
     9121 atttgatcag gctcttcagc cggtccacga tgaagaagtg ctcgtcctcg tcccagtagg
     9181 cgatgtcgcc gctgtgcagc cagccgtcct tgtcgatgag agcgtttgta gcctcggggt
     9241 tgttaacgta gccgctcatg atcatggggc cacggacgca cagctcgccg cgctggttca
     9301 cacccagtgt cttaccggtg tccaagtcca ccaccttagc ctcgaagaag ggcaccacct
     9361 tgcctactgc gccaggcttg tcgtcccctt cgggggtgat cagaatggcg ctggttgttt
     9421 ctgtcaggcc gtagccctgg cggatgcctg gtaggtggaa gcgtttggcc acggcctcac
     9481 ctacctcctt gctgagcggc gccccgccgc tggcgatctc gtgcaagttg cttaggtcgt
     9541 acttgtcgat gagagtgctc ttagcgaaga agctaaatag tgtgggcacc agcagggcag
     9601 attgaatctt atagtcttgc aagctgcgca agaatagctc ctcctcgaag cggtacatga
     9661 gcacgacccg aaagccgcag atcaagtagc ccagcgtggt gaacatgccg aagccgtggt
     9721 gaaatggcac cacgctgagg atagcggtgt cggggatgat ctggttgccg aagatggggt
     9781 cgcgggcatg actgaatcgg acacaagcgg tgcggtgcgg tagggctacg cccttgggca
     9841 atccggtact gccactactg ttcatgatca gggcgatggt tttgtcccgg tcgaagctct
     9901 cgggcacgaa gtcgtactcg ttgaagccgg gtggcaaatg ggaagtcacg aaggtgtaca
     9961 tgctttggaa gccctggtag tcggtcttgc tatccatgat gatgatcttt tgtatgatcg
    10021 gtagcttctt ttgcacgttg aggatctttt gcagcccttt cttgctcacg aatacgacgg
    10081 tgggctggct gatgcccatg ctgttcagca gctcgcgctc gttgtagatg tcgttagctg
    10141 gggccacagc cacaccgatg aacagggcac ccaacacggg catgaagaac tgcaagctat
    10201 tctcgctgca caccacgatc cgatggtttg tattcagccc atagcgcttc atagcttctg
    10261 ccagccgaac gctcatctcg aagtactcgg cgtaggtaat gtccacctcg atatgtgcgt
    10321 cggtaaaggc gatggtgccg ggcaccaggg cgtagcgctt catggctttg tgcagctgct
    10381 cgccggcggt cccgtcttcg agtgggtaga atggcgctgg gcccttctta atgtttttgg
    10441 catcttccat ggcggcgcta ccggtctgtg ttctggcggc aaacccgttg cgaaaaagaa
    10501 cgttcacggc gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga
    10561 gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggatc
    10621 aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccacg
    10681 tggaattatc gattccgcgg tgaattcttc gagacctaga aaaacatgga gcaatcacaa
    10741 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
    10801 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
    10861 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    10921 gaagacaaga tatccttgat ttgtggatct accacacaca aggctacttc cctgattggc
    10981 agaactacac accagggcca gggattagat atccactgac ctttggatgg tgctacaagc
    11041 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
    11101 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
    11161 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
    11221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    11281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    11341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
    11401 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    11461 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    11521 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    11581 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    11641 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
    11701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    11761 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    11821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    11881 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    11941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    12001 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    12061 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    12121 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
    12181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    12241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    12301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    12361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
    12421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
    12481 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
    12541 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
    12601 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
    12661 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
    12721 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
    12781 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
    12841 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
    12901 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    12961 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    13021 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    13081 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13141 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13201 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    13261 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    13321 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    13381 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    13441 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    13501 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    13561 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    13621 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    13681 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    13741 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    13801 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    13861 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    13921 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    13981 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    14041 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    14101 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    14161 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    14221 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    14281 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    14341 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    14401 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    14461 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    14521 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    14581 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    14641 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    14701 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    14761 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    14821 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    14881 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    14941 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    15001 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    15061 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    15121 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    15181 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    15241 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    15301 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    15361 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
