LOCUS       PC1575                  7285 bp ds-DNA     circular SYN 16-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Cornell and University
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 9, 2022 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     misc_feature    complement(879..898)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.2"
                     /Hairpin_Tm="45.7"
                     /Self_Dimer_Tm="6.4"
                     /Sequence="TGGTAAGCTTGGGTCTCCCT"
                     /annotation_group="1056546728430864782d74f7-caa3-442c-8d8d
                     -1b601febdf7curn:sequence:genbank:."
                     /label="898 R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     899..>936
                     /Sequence="TGGGAGACGATTAATGCGTCTCCGGCAGCGGTTCTGGCCGGCTGAAC
                     TTC"
                     /created_by="User"
                     /label
     intron          901..921
                     /created_by="User"
                     /label="Esp3I Cassette"
     overhang        901..921
                     /created_by="User"
                     /label
     misc_feature    901..920
                     /Original_Bases="AGGACCCTGAACGAGATCTACCACTGGTTCACCAGGATGTT
                     CGCCTTCTT"
                     /label="AGGACCCTGAACGAGATCTACCACTGGTTCACCAGGATGTTCGCCTTCTT
                     "
                     /note="Geneious type: Editing History Replacement"
     modified_base   complement(902..907)
                     /created_by="User"
                     /label
     misc_feature    complement(902..907)
                     /created_by="User"
                     /label
                     /note="Geneious type: Inserted sequence"
     misc_feature    915..920
                     /created_by="User"
                     /label
                     /note="Geneious type: Inserted sequence"
     ORF             922..>936
                     /created_by="User"
                     /label
     polylinker      922..936
                     /created_by="User"
                     /label
     source          <937..>1464
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    937..1464
                     /created_by="User"
                     /label="CHIP_TPR"
                     /note="Geneious type: topological domain"
     ORF             <937..>1464
                     /created_by="User"
                     /label
     primer_bind     954..971
                     /created_by="primer3"
                     /%GC="66.7"
                     /Tm="61.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CGACATCCCCAGCGCTCT"
                     /annotation_group="5580787904558326c814f3b-c133-4ed3-b42a-
                     b339d935f6b7urn:sequence:genbank:."
                     /label="984 F"
     misc_feature    983..1416
                     /Original_Bases="GAAGAAGACGCTGGAACAGCATTGAGGAGCGGCGCATCCAC
                     CAGGAGAGCGAGCTGCACTCCTACCTCTCCAGGCTCATTGCCGCGGAGCGTGAGAGGG
                     AGCTGGAAGAGTGCCAGCGAAACCACGAGGGTGATGAGGACGACAGCCACGTCCGGGC
                     CCAGCAGGCCTGCATTGAGGCCAGACACGACAGATACATGGCGGACATGGACGAGCTT
                     TTTTCTCAGGTGGATGAGAGAAGGAGAAGACGAGACATCCCCGACTACCTGTGTGGCA
                     GAATCAGCTTTGAGCTGATGCGGGAGCCGTGCATCACGCCCAGTGGCATCACCTACGA
                     CCGCAGAGACATCGAGGAGCACCTGCAGCGTGTGGGTCATTTTGACCCCGTGACCCGG
                     AGCCCCCTGACCCAGGAACAGCTCATCCCCAACTTGGCTATGAGA"
                     /label="GAAGAAGACGCTGGAACAGCATTGAGGAGCGGCGCATCCACCAGGAGAGC
                     GAGCTGCACTCCTACCTCTCCAGGCTCATTGCCGCGGAGCGTGAGAGGGAGCTGGAAG
                     AGTGCCAGCGAAACCACGAGGGTGATGAGGACGACAGCCACGTCCGGGCCCAGCAGGC
                     CTGCATTGAGGCCAGACACGACAGATACATGGCGGACATGGACGAGCTTTTTTCTCAG
                     GTGGATGAGAGAAGGAGAAGACGAGACATCCCCGACTACCTGTGTGGCAGAATCAGCT
                     TTGAGCTGATGCGGGAGCCGTGCATCACGCCCAGTGGCATCACCTACGACCGCAGAGA
                     CATCGAGGAGCACCTGCAGCGTGTGGGTCATTTTGACCCCGTGACCCGGAGCCCCCTG
                     ACCCAGGAACAGCTCATCCCCAACTTGGCTATGAGA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(1439..1458)
                     /created_by="primer3"
                     /%GC="60.0"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTCCACCCAGCCATTCTCAG"
                     /annotation_group="558104253661039e9ebedab-b35c-4704-981d-
                     a7be949e635durn:sequence:genbank:."
                     /label="1,488 R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1439..1458
                     /Sequence="CTGAGAATGGCTGGGTGGAG"
                     /Hairpin_Tm="None"
                     /Tm="60.1"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /modified_by="User"
                     /label="1,469 F"
     CDS             1477..1494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /translation="HHHHHH"
                     /label="6xHis"
     misc_feature    1495..1497
                     /label="Stop"
     misc_feature    1498..1503
                     /label="EcoRI cut site"
     misc_feature    1504..2088
                     /label="IRES2"
     misc_feature    complement(1540..1559)
                     /Sequence="GCCTTATTCCAAGCGGCTTC"
                     /Hairpin_Tm="32.4"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /modified_by="User"
                     /label="IRES_R"
                     /note="Geneious type: primer_bind_reverse"
     CDS             2092..2802
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
                     /label="mCherry"
     misc_feature    complement(2239..2258)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCCTTGGTCACCTTCAGCTT"
                     /annotation_group="88298945964400182e82c7a-14d0-4f22-9108-
                     ce0a41d27af4urn:sequence:genbank:."
                     /label="2,288 R"
                     /note="Geneious type: primer_bind_reverse"
     Polymorphism    2800..2802
                     /label
     promoter        complement(2837..2855)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     polyA_signal    2881..3105
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3151..3579
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3593..3923
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      3774..3909
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     CDS             3990..4784
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and
                     G418(Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /label="NeoR/KanR"
     polyA_signal    4958..5079
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5128..5144)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     protein_bind    5152..5168
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5176..5206)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5221..5242
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5530..6118)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     CDS             complement(6289..7149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     promoter        complement(7150..7254)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttaccatg
      901 ggagacgatt aatgcgtctc cggcagcggt tctggccggc tgaacttcgg ggacgacatc
      961 cccagcgctc ttcgaatcgc gaagaagaag cgctggaaca gcattgagga gcggcgcatc
     1021 caccaggaga gcgagctgca ctcctacctc tccaggctca ttgccgcgga gcgtgagagg
     1081 gagctggaag agtgccagcg aaaccacgag ggtgatgagg acgacagcca cgtccgggcc
     1141 cagcaggcct gcattgaggc caagcacgac aagtacatgg cggacatgga cgagcttttt
     1201 tctcaggtgg atgagaagag gaagaagcga gacatccccg actacctgtg tggcaagatc
     1261 agctttgagc tgatgcggga gccgtgcatc acgcccagtg gcatcaccta cgaccgcaag
     1321 gacatcgagg agcacctgca gcgtgtgggt cattttgacc ccgtgacccg gagccccctg
     1381 acccaggaac agctcatccc caacttggct atgaaggagg ttattgacgc attcatctct
     1441 gagaatggct gggtggagga ttacgtcgac ggagcacatc atcatcatca tcactgagaa
     1501 ttcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     1561 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     1621 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     1681 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     1741 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     1801 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     1861 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     1921 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     1981 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     2041 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
     2101 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     2161 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     2221 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     2281 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     2341 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2401 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     2461 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     2521 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     2581 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     2641 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     2701 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     2761 ggccgccact ccaccggcgg catggacgag ctgtacaagt gagcggccgc tcgagcatgc
     2821 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     2881 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2941 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3001 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3061 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3121 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3181 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3241 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3301 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3361 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3421 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3481 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt
     3541 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     3601 gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc
     3661 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3721 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3781 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3841 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     3901 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     3961 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4021 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4081 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4141 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4201 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4261 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4321 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4381 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4441 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4501 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4561 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4621 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4681 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4741 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     4801 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     4861 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4921 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     4981 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5041 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5101 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5161 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5221 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5701 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     5761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5941 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     6001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7261 tccccgaaaa gtgccacctg acgtc
//
