LOCUS       Exported               11324 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      773..1361
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1544..1636
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1649..1670
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1685..1715
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1719..1741
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     promoter        1784..1802
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1930..2273
                     /label=TEF promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2274..2927
                     /codon_start=1
                     /label=S. pombe his5
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      2933..3130
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     promoter        3246..3264
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             3267..3424
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.8"
                     /note="/other=CDS"
                     /translation="IHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPN
                     SCAA*MA"
     rep_origin      3428..3856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    3884..3935
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     CDS             complement(4231..5187)
                     /codon_start=1
                     /label=RT86_ECOLX
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
                     /translation="KSAEYLNTFRLRNLGLPVMNNLHDMSKATRISVETLRLLIYTADF
                     RYRIYTVEKKGPEKRMRTIYQPSRELKALQGWVLRNILDKLSSSPFSIGFEKHQSILNN
                     ATPHIGANFILNIDLEDFFPSLTANKVFGVFHSLGYNRLISSVLTKICCYKNLLPQGAP
                     SSPKLANLICSKLDYRIQGYAGSRGLIYTRYADDLTLSAQSMKKVVKARDFLFSIIPSE
                     GLVINSKKTCISGPRSQRKVTGLVISQEKVGIGREKYKEIRAKIHHIFCGKSSEIEHVR
                     GWLSFILSVDSKSHRRLITYISKLEKKYGKNPLNKAKT"
     promoter        5226..5878
                     /label=GAL1,10 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=96.8"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    5879..5897
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.6"
                     /note="/other=misc_feature"
     CDS             5904..10007
                     /codon_start=1
                     /label=cas9
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      10056..10300
                     /label=CYC1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=99.2"
                     /note="/other=terminator"
     CDS             complement(10320..10355)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     misc_feature    10423..10924
                     /label=CEN/ARS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        10961..11065
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(11066..11324,1..602)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
       61 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      121 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      181 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      241 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
      301 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      361 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
      421 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
      481 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      541 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      601 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      661 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      721 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      781 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      841 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      901 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      961 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1021 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1081 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1141 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1201 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1261 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1321 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1381 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1441 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1501 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1561 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1621 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1681 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1741 ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa
     1801 gggaacaaaa gctgctagag cggccgccag ctgaagcttc gtacgctgca ggtcgacgga
     1861 tccccgggtt aattaaggcg cgccagatct gtttagcttg cctcgtcccc gccgggtcac
     1921 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
     1981 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
     2041 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag
     2101 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
     2161 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
     2221 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta
     2281 ggagggcttt tgtagaaaga aatacgaacg aaacgaaaat cagcgttgcc atcgctttgg
     2341 acaaagctcc cttacctgaa gagtcgaatt ttattgatga acttataact tccaagcatg
     2401 caaaccaaaa gggagaacaa gtaatccaag tagacacggg aattggattc ctggatcaca
     2461 tgtatcatgc actggctaaa catgcaggct ggagcttacg actttactca agaggtgatt
     2521 taatcatcga tgatcatcac actgcagaag atactgctat tgcacttggt attgcattca
     2581 agcaggctat gggtaacttt gccggcgtta aaagatttgg acatgcttat tgtccacttg
     2641 acgaagccct ttctagaagc gtagttgact tgtcgggacg gccctatgct gttatcgatt
     2701 tgggattaaa gcgtgaaaag gttggggaat tgtcctgtga aatgatccct cacttactat
     2761 attccttttc ggtagcagct ggaattactt tgcatgttac ctgcttatat ggtagtaatg
     2821 accatcatcg tgctgaaagc gcttttaaat ctctggctgt tgccatgcgc gcggctacta
     2881 gtcttactgg aagttctgaa gtcccaagca cgaagggagt gttgtaaaga gtactgacaa
     2941 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt
     3001 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc
     3061 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt
     3121 cgctatactg ctgtcgattc gatactaacg ccgccatcca gttcgaaaac gagctcgaat
     3181 tcatcgatga tatcagatcc actagtggcc tatgcggccg ccaccgcggt ggagctccaa
     3241 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     3301 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3361 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3421 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3721 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3781 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3841 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     3901 tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt
     3961 gaagctctaa tttgtgagtt tagtatacat gcatgcgaat tgcatatctt tccatagatt
     4021 ttcaattttt gatgtctcca tggtggtaca gagaacttgt aaacaattcg gtccctacat
     4081 gtgaggaaat tcgctgtgac acttttatca ctgaactcca aatttaaaaa atagcataaa
     4141 attcgttata cagcaaatct atgtgttgca attaagaact aaaagatata gagtgcatat
     4201 tttcaagaag gatagtaagc tggcaaatca agtcttagcc ttgttcaatg ggttcttacc
     4261 gtacttcttt tccaacttag agatgtaggt aatcaatctt ctgtgggact tagaatcaac
     4321 ggacaagatg aaagacaacc aacctctaac atgttcgatt tcggaggatt taccgcagaa
     4381 aatatggtgg atcttggctc tgatttcttt gtacttttct ctaccaatac caaccttttc
     4441 ttgggagata accaaaccgg taacttttct ttgggatctt ggaccagaaa tacaggtctt
     4501 tttggagttg atgaccaaac cttctgatgg aataatggag aacaagaagt ctctggcctt
     4561 aacaactttc ttcatagatt gagcagacaa ggtcaagtca tcagcgtatc tagtgtatat
     4621 caaacctcta gaaccagcat aaccttgaat tctgtagtcc aacttggagc aaatcaagtt
     4681 agccaatttt ggagaagatg gagcaccttg tggcaacaag ttcttgtagc agcaaatctt
     4741 ggtcaaaaca gaggagatca atctgttgta acccaaagaa tggaaaacac cgaaaacctt
     4801 gttagcagtc aaagatggga agaaatcttc caagtcgatg ttcaaaatga agttggcacc
     4861 aatatgtgga gtggcattgt tcaagatgga ttggtgcttt tcaaaaccga tagaaaatgg
     4921 ggaggaggat aacttgtcca agatgtttct caaaacccaa ccttgtaaag ccttcaattc
     4981 tctggatggt tggtagatag ttctcattct cttttctgga cccttctttt caacagtgta
     5041 gattctgtat ctgaaatcgg cggtgtagat caataatctc aaggtttcaa cagaaattct
     5101 ggtagcctta gacatatcgt gcaagttgtt catgactggc aaacccaaat ttctcaatct
     5161 gaaggtgttc aagtattcag cggacttgac ctttcttttc ttctttggtg gcatttatat
     5221 tgaattttca aaaattctta cttttttttt ggatggacgc aaagaagttt aataatcata
     5281 ttacatggca ttaccaccat atacatatcc atatctaatc ttacttatat gttgtggaaa
     5341 tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt tcagtaatac
     5401 gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg ggcgacagcc
     5461 ctccgacgga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc
     5521 agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg
     5581 gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat taacgaatca
     5641 aattaacaac cataggatga taatgcgatt agttttttag ccttatttct ggggtaatta
     5701 atcagcgaag cgatgatttt tgatctatta acagatatat aaatggaaaa gctgcataac
     5761 cactttaact aatactttca acattttcag tttgtattac ttcttattca aatgtcataa
     5821 aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga gaaaaaacaa
     5881 ctagtggatc ccccgggaaa aaaatggaca agaagtactc cattgggctc gatatcggca
     5941 caaacagcgt cggttgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca
     6001 aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt
     6061 tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata
     6121 cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg
     6181 tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc
     6241 acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc
     6301 caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt
     6361 tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg
     6421 acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca
     6481 atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga
     6541 gcgctaggct gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga
     6601 agaagaacgg cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta
     6661 aatctaactt cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg
     6721 atgatctcga caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg
     6781 caaagaacct gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca
     6841 ccaaagctcc gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga
     6901 ctttgctgaa ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg
     6961 atcagtctaa aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt
     7021 acaaatttat taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc
     7081 ttaacagaga agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc
     7141 agattcacct gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt
     7201 tgaaagataa cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag
     7261 gccccctcgc ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacca
     7321 tcactccctg gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg
     7381 aaaggatgac taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc
     7441 tgctgtacga gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag
     7501 ggatgagaaa gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct
     7561 tcaagacgaa ccggaaagtt accgtgaaac agctcaaaga agactatttc aaaaagattg
     7621 aatgtttcga ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa
     7681 cgtatcacga tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg
     7741 aggacattct tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg
     7801 aagaacgctt gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga
     7861 ggcgccgata tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca
     7921 agcagagtgg aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact
     7981 tcatgcagtt gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag
     8041 tttctggcca gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta
     8101 tcaaaaaggg aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa
     8161 ggcataagcc cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg
     8221 gacagaagaa cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt
     8281 cccaaatcct taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc
     8341 tgtactacct gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc
     8401 tctccgacta cgacgtggat catatcgtgc cccagtcttt tctcaaagat gattctattg
     8461 ataataaagt gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag
     8521 aagaagttgt caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca
     8581 cacaacggaa gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata
     8641 aagccggctt catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc
     8701 aaattctcga ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg
     8761 tgaaagttat tactctgaag tctaagctgg tctcagattt cagaaaggac tttcagtttt
     8821 ataaggtgag agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg
     8881 taggcactgc acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact
     8941 ataaagtgta cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca
     9001 ccgctaagta cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg
     9061 ccaatggaga gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg
     9121 tgtgggacaa gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga
     9181 acatcgttaa aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga
     9241 aaaggaacag cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg
     9301 gattcgattc tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga
     9361 agtctaaaaa actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa
     9421 gcttcgaaaa aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag
     9481 acctcatcat taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa
     9541 tgctcgctag tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg
     9601 ttaatttctt gtatctggcc agccactatg aaaagctcaa agggtctccc gaagataatg
     9661 agcagaagca gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa
     9721 taagcgaatt ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg
     9781 cttacaataa gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt
     9841 ttactctgac caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca
     9901 gaaagcggta cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta
     9961 cggggctcta tgaaacaaga atcgacctct ctcagctcgg tggagacagc agggctgacc
    10021 ccaagaagaa gaggaaggtg tgatctcttc tcgagtcatg taattagtta tgtcacgctt
    10081 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
    10141 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
    10201 tcaaattttt ctttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
    10261 ttgcttgaga aggttttggg acgctcgaag gctttaattt catccgggag ctgcatgtgt
    10321 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
    10381 tttttatagg ttaatgtcat gataataatg gtttcttaga cggatcgctt gcctgtaact
    10441 tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct gtgtttattt
    10501 atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa gagttacgga
    10561 atgaagaaaa aaataaacaa aggtttaaaa aatttcaaca aaaagcgtac tttacatata
    10621 tatttattag acaagaaaag cagattaaat agatatacat tcgattaacg ataagtaaaa
    10681 tgtaaaatca caggattttc gtgtgtggtc ttctacacag acaagatgaa acaattcggc
    10741 attaatacct gagagcagga agagcaagat aaaaggtagt atttgttggc gatcccccta
    10801 gagtctttta catcttcgga aaacaaaaac tattttttct ttaatttctt tttttacttt
    10861 ctatttttaa tttatatatt tatattaaaa aatttaaatt ataattattt ttatagcacg
    10921 tgatgaaaag gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    10981 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    11041 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    11101 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    11161 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    11221 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    11281 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caag
//
