LOCUS       Exported               13775 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     43..59
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     81..100
                     /label=T3 promoter
     gene            226..1801
                     /label=hphMX6
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=gene"
     promoter        226..569
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     249..268
                     /label=TEF forward primer
     CDS             570..1598
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      1604..1801
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1731..1750)
                     /label=TEF reverse primer
     promoter        complement(1918..1936)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     complement(1943..1960)
                     /label=M13 forward
     rep_origin      2100..2555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2667..4687
                     /label=ACT1pr-yZ3EV-3'UTR
     CDS             complement(4930..5889)
                     /codon_start=1
                     /label=RT86_ECOLX
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VKSAEYLNTFRLRNLGLPVMNNLHDMSKATRISVETLRLLIYTAD
                     FRYRIYTVEKKGPEKRMRTIYQPSRELKALQGWVLRNILDKLSSSPFSIGFEKHQSILN
                     NATPHIGANFILNIDLEDFFPSLTANKVFGVFHSLGYNRLISSVLTKICCYKNLLPQGA
                     PSSPKLANLICSKLDYRIQGYAGSRGLIYTRYADDLTLSAQSMKKVVKARDFLFSIIPS
                     EGLVINSKKTCISGPRSQRKVTGLVISQEKVGIGREKYKEIRAKIHHIFCGKSSEIEHV
                     RGWLSFILSVDSKSHRRLITYISKLEKKYGKNPLNKAKT"
     misc_feature    5925..6623
                     /label=Z3 promoter
     CDS             6649..10752
                     /codon_start=1
                     /label=cas9
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10765..10785
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      10847..11036
                     /label=CYC1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(11169..11671)
                     /label=CEN/ARS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        11708..11812
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             11813..12673
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12844..13432
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
       61 atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg ctagagcggc
      121 cgccagctga agcttcgtac gctgcaggtc gacggatccc cgggttaatt aaggcgcgcc
      181 agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag
      241 aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg
      301 tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc
      361 acggcgcgaa gcaaaaatta cggctcctcg ctgcggacct gcgagcaggg aaacgctccc
      421 ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag
      481 gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt
      541 tctcacatca catccgaaca taaacaacca tgggtaaaaa gcctgaactc accgcgacgt
      601 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
      661 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
      721 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
      781 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
      841 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
      901 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
      961 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     1021 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     1081 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     1141 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     1201 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     1261 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     1321 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     1381 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     1441 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     1501 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     1561 accgacgccc cagcactcgt ccgagggcaa aggaataatc agtactgaca ataaaaagat
     1621 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
     1681 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
     1741 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
     1801 gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctcga attcatcgat
     1861 gatatcagat ccactagtgg cctatgcggc cgccaccgcg gtggagctcc aattcgccct
     1921 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1981 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2041 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     2101 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2161 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2221 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2281 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2341 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2401 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2461 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2521 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     2581 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct
     2641 aatttgtgag tttagtatac atgcatgcgt cgatctcccc tcaagtggca aactgcgact
     2701 gatatgaaag taaagtatcg gaccttctat tacggaatgt atacaaacgc tacaggatac
     2761 agtgatatta aatagaaaac aagtctacaa ttaaatggca gaaagaagaa ccagcttact
     2821 actaaccacc atactcatca atacccagag catcggtgaa catctgctca aattcgaagt
     2881 cagccatatc cagcgcaccg taaggtgctg aatcatgtgg agtgaagcca ggccctggag
     2941 agtccccgtc acccaacata tctaaatcaa aatcgtctaa agcgtcagcg tgggccatgg
     3001 caacgtcttc accgtccaaa tgcagttcgc tagatgtaga ccctgctgtc gctaggtggg
     3061 attggtcggt ttcttcaacg gaggctccgc ctctagaagt tggtgcatgc aacctgtgag
     3121 cgtccagcat ttctagtaat aaatcataca gaggaacgac gtttttacac ttcatagaat
     3181 acaggtgctc catacccttg ttagacatat gtctaatgtg tgacaaaatt agtagtaact
     3241 gagctagcct ctgatgctgt tgctgcagag tcaatcctgc tttcgccata aggtgaatca
     3301 atgtatcagt gatcttgtcc aacactctat gtatatggtc tttctcttcc aggcttttca
     3361 gagttgagct taaaaatgta tagacaccgc tatttaacaa aataattgat ttcaggcaaa
     3421 cgaattcttc tccttgcaaa ttcatcattc tgaacctgga gctagtggca agaagcatat
     3481 caaaaatctc aaccatacct tctacgcatt taccttggtt cctgtccaat aataaattag
     3541 gtgcaaataa taattttaca gggtgctcca tacttctcca cactaatccg atcatcaata
     3601 tttctaacca cgcgcattca agaagatgta cctggtcatg taatgtcaag tcaacaaagc
     3661 cgggaactct tttggcccaa ttaatcatat gaactaattc cctgtctgct agatttgtca
     3721 aaagacccat catggaagcc tcggaaaaag gacgagttgg gtcgtattca ctgtacaata
     3781 taggtggttc agcatcaagt agtgcggaaa ccatttgatc agcggtcaaa gacagtgcaa
     3841 gagaattctt cttagatctt ttgatcatta atgggcttgg ccacaagttg gctgccctca
     3901 tgtctccagc gcttggatct tctagggttg aggctgctgc cggagtgccc ccacctcctg
     3961 tatggatctt tgtatgtctc tttctttcat cagatctggc gaactttctt ccacaaatgt
     4021 cacacgcgaa cggcttctcg ccggtgtggg ttctaatgtg cgtagtcaaa tggtcacttc
     4081 tgctgaagtt tctcatacag attctacatt ggaaaggctt ttggccggtg tgaatacgaa
     4141 tatgcctagt cagctcgtca gatctgctga atctcctatc acaactttct acagggcaag
     4201 cgtagggtct cgttcccatt gttaattcag taaattttcg atcttgggaa gaaaaaagca
     4261 gtaagcgtga aaaatctaaa agctgatgta gtagaagatc ctattcttta acaaagattg
     4321 accttttctt tttcttcttg gtttgagtag aaaggggaag gaagaataca agagagagga
     4381 aaaaaaggaa gataaaaaga gagcgtgata taaatgaata tatattaaac aagagagatt
     4441 gggaaggaaa ggatcaaaca aacccaaaaa tatttcaaaa aggagagaga gaggcgagtt
     4501 tggtttcaaa acggtttatt tatttatgca agaggacgtg gaagaaaaag aagaaggaag
     4561 aaaaaaattt gaaagaaaaa aacgcgtggc gggtaaagaa gaaaatggaa aatagaggcc
     4621 gggtgacaga gaaatattga gggttaattg gaaaatatgt tagggtgagg catatgtttt
     4681 taaagggatg catgcgaatt gcatatcttt ccatagattt tcaatttttg atgtctccat
     4741 ggtggtacag agaacttgta aacaattcgg tccctacatg tgaggaaatt cgctgtgaca
     4801 cttttatcac tgaactccaa atttaaaaaa tagcataaaa ttcgttatac agcaaatcta
     4861 tgtgttgcaa ttaagaacta aaagatatag agtgcatatt ttcaagaagg atagtaagct
     4921 ggcaaatcaa gtcttagcct tgttcaatgg gttcttaccg tacttctttt ccaacttaga
     4981 gatgtaggta atcaatcttc tgtgggactt agaatcaacg gacaagatga aagacaacca
     5041 acctctaaca tgttcgattt cggaggattt accgcagaaa atatggtgga tcttggctct
     5101 gatttctttg tacttttctc taccaatacc aaccttttct tgggagataa ccaaaccggt
     5161 aacttttctt tgggatcttg gaccagaaat acaggtcttt ttggagttga tgaccaaacc
     5221 ttctgatgga ataatggaga acaagaagtc tctggcctta acaactttct tcatagattg
     5281 agcagacaag gtcaagtcat cagcgtatct agtgtatatc aaacctctag aaccagcata
     5341 accttgaatt ctgtagtcca acttggagca aatcaagtta gccaattttg gagaagatgg
     5401 agcaccttgt ggcaacaagt tcttgtagca gcaaatcttg gtcaaaacag aggagatcaa
     5461 tctgttgtaa cccaaagaat ggaaaacacc gaaaaccttg ttagcagtca aagatgggaa
     5521 gaaatcttcc aagtcgatgt tcaaaatgaa gttggcacca atatgtggag tggcattgtt
     5581 caagatggat tggtgctttt caaaaccgat agaaaatggg gaggaggata acttgtccaa
     5641 gatgtttctc aaaacccaac cttgtaaagc cttcaattct ctggatggtt ggtagatagt
     5701 tctcattctc ttttctggac ccttcttttc aacagtgtag attctgtatc tgaaatcggc
     5761 ggtgtagatc aataatctca aggtttcaac agaaattctg gtagccttag acatatcgtg
     5821 caagttgttc atgactggca aacccaaatt tctcaatctg aaggtgttca agtattcagc
     5881 ggacttgacc tttcttttct tctttggtgg catttatatt gaatttatat tgaattttca
     5941 aaaattctta cttttttttt ggatggacgc aaagaagttt aataatcata ttacatggca
     6001 ttaccaccat atacatatcc atatacatat ccatatctaa tcttacttat atgttgtgga
     6061 aatgtaaaga gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat
     6121 acgcttaact gctcattgct atattgaagt gcggccgcgt gggcgtgcgt gggcgggcgt
     6181 gggcgtgcgt gggcgggcgt gggcgtgcgt gggcgtctag accgtgcgtc ctcgtcttca
     6241 ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat
     6301 tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca
     6361 aaccttcaaa ttaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta
     6421 gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata
     6481 taaatggaaa agctgcataa ccactttaac taatactttc aacattttca gtttgtatta
     6541 cttcttattc aaatgtcata aaagtatcaa caaaaaattg ttaatatacc tctatacttt
     6601 aacgtcaagg agaaaaaact ataaactagt ggatcccccg ggaaaaaaat ggacaagaag
     6661 tactccattg ggctcgatat cggcacaaac agcgtcggtt gggccgtcat tacggacgag
     6721 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag
     6781 aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc
     6841 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag
     6901 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc
     6961 tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac
     7021 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac
     7081 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt
     7141 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc
     7201 tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc
     7261 ggagttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac
     7321 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg
     7381 tcactcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt
     7441 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac
     7501 cagtacgcag accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat
     7561 attctgcgag tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc
     7621 tatgatgagc accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct
     7681 gagaagtaca aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac
     7741 ggcggagcaa gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac
     7801 ggcaccgagg agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact
     7861 ttcgacaatg gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg
     7921 cggcaagagg atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc
     7981 acatttcgga taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg
     8041 atgactcgca aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag
     8101 ggggcctctg cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac
     8161 gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc
     8221 accaaggtca aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag
     8281 aagaaagcta tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc
     8341 aaagaagact atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag
     8401 gatcgcttca acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag
     8461 gacttcctgg acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg
     8521 ttgtttgaag atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac
     8581 gacaaagtca tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga
     8641 aaactgatca atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag
     8701 tccgatggat ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt
     8761 aaggaggaca tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc
     8821 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg
     8881 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc
     8941 cgagagaacc aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt
     9001 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc
     9061 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg
     9121 gatcaggaac tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag
     9181 tcttttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaataga
     9241 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg
     9301 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa
     9361 cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca
     9421 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat
     9481 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca
     9541 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg
     9601 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt
     9661 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag
     9721 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat
     9781 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa
     9841 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg
     9901 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc
     9961 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa
    10021 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg
    10081 gttgtggcca aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg
    10141 ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg
    10201 aaaggatata aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt
    10261 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac
    10321 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag
    10381 ctcaaagggt ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac
    10441 taccttgatg agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac
    10501 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag
    10561 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc
    10621 aagtacttcg acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac
    10681 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag
    10741 ctcggtggag acagcagggc tgaccccaag aagaagagga aggtgtgatc tcttctcgag
    10801 tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg
    10861 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt
    10921 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt
    10981 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt
    11041 taatttcatc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
    11101 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
    11161 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
    11221 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
    11281 aataaagaag gtagaagagt tacggaatga agaaaaaaaa taaacaaagg tttaaaaaat
    11341 ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga
    11401 tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc
    11461 tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga gcaagataaa
    11521 aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat
    11581 tttttcttta atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat
    11641 ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg
    11701 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    11761 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    11821 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    11881 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    11941 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    12001 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    12061 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    12121 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    12181 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    12241 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    12301 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    12361 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    12421 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    12481 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    12541 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    12601 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    12661 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    12721 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    12781 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    12841 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    12901 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    12961 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    13021 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    13081 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    13141 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    13201 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    13261 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    13321 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    13381 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    13441 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    13501 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    13561 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    13621 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    13681 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    13741 ttaggcaccc caggctttac actttatgct tccgg
//
