LOCUS       Exported                9449 bp ds-DNA     circular SYN 24-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9449
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    350..1925
                     /label=HPHMX6 cassette
     terminator      complement(350..547)
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(554..1575)
                     /codon_start=1
                     /label=HygR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     promoter        complement(1582..1925)
                     /label=TEF promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1968..2034
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=CDS"
                     /translation="LA**RRGPHRSPFPTVAQPEWR"
     rep_origin      2038..2466
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(2624..3427)
                     /codon_start=1
                     /label=URA3
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3428..3643)
                     /label=URA3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(3905..4408)
                     /label=CEN/ARS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4445..4549
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4550..5410
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5581..6169
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    6229..8249
                     /label=ACT1pr-yZ3EV-3'UTR
                     /note="Yeast codon-optimized Z3EV transcription factorunder
                     the control of the ACT1 promoter; has the A. gossypii TEF3 
                     3'UTR"
     misc_feature    8256..8954
                     /label=Z3 promoter
     misc_feature    8999..9096
                     /label=Retron Ec86
     misc_feature    9097..9102
                     /label=XhoI cloning site
     misc_feature    9103..9264
                     /label=sgRNA and HDV
     misc_feature    join(9265..9449,1..170)
                     /label=tGAL7
ORIGIN
        1 aaaaaaaaaa aaagaaaaaa gaattttagg aatacaatgc agcttgtaag taaaatctgg
       61 aatattcata tcgccacaac ttcttatgct tataaaagca ctaatgcctg aatttatgtt
      121 gaaaatatgt gtcacaaata aagaaactgt gacatctgac acatttccac gtacccaatt
      181 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagtc
      301 gagagctcgt tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga
      361 ccagcattca catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg
      421 ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa
      481 caactacaat ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat
      541 tgtcagtact gattattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
      601 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
      661 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
      721 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
      781 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
      841 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
      901 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
      961 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     1021 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     1081 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     1141 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     1201 atcggccgca gcaatggcat ccattgcctc cgcgaccggc tgcagaacag cgggcagttc
     1261 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     1321 gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     1381 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     1441 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     1501 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     1561 gagttcaggc tttttaccca tggttgttta tgttcggatg tgatgtgaga actgtatcct
     1621 agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt
     1681 atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt
     1741 ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca
     1801 aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag
     1861 tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc
     1921 atgtcgctgg ccgggtgacc cggcggggac gaggcaagct aaacagactg gcgtaatagc
     1981 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     2041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2221 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2461 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa
     2581 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
     2641 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
     2701 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca
     2761 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac
     2821 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa
     2881 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt
     2941 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt
     3001 cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac
     3061 cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca
     3121 atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact
     3181 tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat
     3241 ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt
     3301 ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat
     3361 taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt
     3421 tcgacatgat ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg gttaagaata
     3481 ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt
     3541 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa
     3601 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac
     3661 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3721 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3781 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3841 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3901 gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
     3961 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
     4021 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
     4081 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
     4141 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
     4201 acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg
     4261 tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt
     4321 ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta
     4381 aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa
     4441 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4501 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4561 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4621 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4681 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4741 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4801 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4861 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4921 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4981 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5041 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5101 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5161 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5221 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5281 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5341 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5401 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5461 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5521 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5581 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5641 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5701 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5761 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5821 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5881 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5941 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6001 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6061 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa aggccagcaa
     6181 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcgctcgc gtcgatctcc
     6241 cctcaagtgg caaactgcga ctgatatgaa agtaaagtat cggaccttct attacggaat
     6301 gtatacaaac gctacaggat acagtgatat taaatagaaa acaagtctac aattaaatgg
     6361 cagaaagaag aaccagctta ctactaacca ccatactcat caatacccag agcatcggtg
     6421 aacatctgct caaattcgaa gtcagccata tccagcgcac cgtaaggtgc tgaatcatgt
     6481 ggagtgaagc caggccctgg agagtccccg tcacccaaca tatctaaatc aaaatcgtct
     6541 aaagcgtcag cgtgggccat ggcaacgtct tcaccgtcca aatgcagttc gctagatgta
     6601 gaccctgctg tcgctaggtg ggattggtcg gtttcttcaa cggaggctcc gcctctagaa
     6661 gttggtgcat gcaacctgtg agcgtccagc atttctagta ataaatcata cagaggaacg
     6721 acgtttttac acttcataga atacaggtgc tccataccct tgttagacat atgtctaatg
     6781 tgtgacaaaa ttagtagtaa ctgagctagc ctctgatgct gttgctgcag agtcaatcct
     6841 gctttcgcca taaggtgaat caatgtatca gtgatcttgt ccaacactct atgtatatgg
     6901 tctttctctt ccaggctttt cagagttgag cttaaaaatg tatagacacc gctatttaac
     6961 aaaataattg atttcaggca aacgaattct tctccttgca aattcatcat tctgaacctg
     7021 gagctagtgg caagaagcat atcaaaaatc tcaaccatac cttctacgca tttaccttgg
     7081 ttcctgtcca ataataaatt aggtgcaaat aataatttta cagggtgctc catacttctc
     7141 cacactaatc cgatcatcaa tatttctaac cacgcgcatt caagaagatg tacctggtca
     7201 tgtaatgtca agtcaacaaa gccgggaact cttttggccc aattaatcat atgaactaat
     7261 tccctgtctg ctagatttgt caaaagaccc atcatggaag cctcggaaaa aggacgagtt
     7321 gggtcgtatt cactgtacaa tataggtggt tcagcatcaa gtagtgcgga aaccatttga
     7381 tcagcggtca aagacagtgc aagagaattc ttcttagatc ttttgatcat taatgggctt
     7441 ggccacaagt tggctgccct catgtctcca gcgcttggat cttctagggt tgaggctgct
     7501 gccggagtgc ccccacctcc tgtatggatc tttgtatgtc tctttctttc atcagatctg
     7561 gcgaactttc ttccacaaat gtcacacgcg aacggcttct cgccggtgtg ggttctaatg
     7621 tgcgtagtca aatggtcact tctgctgaag tttctcatac agattctaca ttggaaaggc
     7681 ttttggccgg tgtgaatacg aatatgccta gtcagctcgt cagatctgct gaatctccta
     7741 tcacaacttt ctacagggca agcgtagggt ctcgttccca ttgttaattc agtaaatttt
     7801 cgatcttggg aagaaaaaag cagtaagcgt gaaaaatcta aaagctgatg tagtagaaga
     7861 tcctattctt taacaaagat tgaccttttc tttttcttct tggtttgagt agaaagggga
     7921 aggaagaata caagagagag gaaaaaaagg aagataaaaa gagagcgtga tataaatgaa
     7981 tatatattaa acaagagaga ttgggaagga aaggatcaaa caaacccaaa aatatttcaa
     8041 aaaggagaga gagaggcgag tttggtttca aaacggttta tttatttatg caagaggacg
     8101 tggaagaaaa agaagaagga agaaaaaaat ttgaaagaaa aaaacgcgtg gcgggtaaag
     8161 aagaaaatgg aaaatagagg ccgggtgaca gagaaatatt gagggttaat tggaaaatat
     8221 gttagggtga ggcatatgtt tttaaaggga ctagtttata ttgaattttc aaaaattctt
     8281 actttttttt tggatggacg caaagaagtt taataatcat attacatggc attaccacca
     8341 tatacatatc catatacata tccatatcta atcttactta tatgttgtgg aaatgtaaag
     8401 agccccatta tcttagccta aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac
     8461 tgctcattgc tatattgaag tgcggccgcg tgggcgtgcg tgggcgggcg tgggcgtgcg
     8521 tgggcgggcg tgggcgtgcg tgggcgtcta gaccgtgcgt cctcgtcttc accggtcgcg
     8581 ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata
     8641 ctagctttta tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa
     8701 attaacgaat caaattaaca accataggat gataatgcga ttagtttttt agccttattt
     8761 ctggggtaat taatcagcga agcgatgatt tttgatctat taacagatat ataaatggaa
     8821 aagctgcata accactttaa ctaatacttt caacattttc agtttgtatt acttcttatt
     8881 caaatgtcat aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag
     8941 gagaaaaaac tataagggtg cgcatctgat gagtccgtga ggacgaaacg agctagctcg
     9001 tcatgcgcac ccttagcgag aggtttatca ttaaggtcaa cctctggatg ttgtttcggc
     9061 atcctgcatt gaatctgagt tactgtctgt tttcctctcg aggtttcaga gctatgctgg
     9121 aaacagcata gcaagttgaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     9181 gtcggtgctt tttgatggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac
     9241 accttcgggt ggcgaatggg acttaaagaa agtggaatat tcattcatat catatttttt
     9301 ctattaactg cctggtttct tttaaatttt ttattggttg tcgacttgaa cggagtgaca
     9361 atatatatat atatatttaa taatgacatc attatctgta aatctgattc ttaatgctat
     9421 tctagttatg taagagtggt cctttccat
//
