LOCUS       plasmid                 4530 bp    DNA     circular SYN 27-NOV-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(join(4215..4530,1..113))
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3080..3871)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2199..3059)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1440..2028)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(1086..1116)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1131..1152)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(1002..1020)
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        277..295
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1062..1078)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(117..274)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             366..917
                     /note="pLannotate"
                     /label="MOGAT2 (fragment)"
                     /database="swissprot"
                     /identity="62.0"
                     /match_length="55.1"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3903..4205)
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="98.0"
                     /match_length="21.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1165..1257)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(3060..3076)
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="26.6"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
       61 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
      121 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      181 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      241 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
      301 ttgggccctc tagatgcatg ctcgagcggc cgccagtgtg atggatatct gcagaattcg
      361 cccttgctgg ttggctgtat ctggaccggg acacgccctc atgtggaggt cggaggtcac
      421 agtgggtcag aagctggagg atctggacgc atttcaggga ttatttccca atcacactgg
      481 tcaagacggt ggatctggac ccgaggcaca attatctgct gggtttccat ccgcacggtg
      541 ttctggtggc cggtggattc ggaaacttct gcacggaggc gtccggattc tcccagatgt
      601 tcccgggact cacgccgtac ctgctgatgc tgccgttctg gttcagggtg cccttcttca
      661 gagaatacat catgtgcgga gggctggtgt ccagtgagaa ggccagtgcg agttacctgc
      721 tgggtcatcc aggcgggggt caagcggccg tcattgcagt gggcggagct ccggagtctc
      781 tggaggcgcg gcctggagca ctgacactgc aactgctgca gcgcaaaggc ttcatcaaac
      841 tcgccctcaa acacggagcg tggctggttc cagtgttctc gtttggggag aacgagctct
      901 tcgaccagat ggagaatccc gcaagggcga attccagcac actggcggcc gttactagtg
      961 gatccgagct cggtaccaag cttgatgcat agcttgagta ttctatagtg tcacctaaat
     1021 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1081 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1141 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1201 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1261 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1321 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1381 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1441 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1501 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1561 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1621 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1681 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1801 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1861 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1921 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1981 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2041 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2101 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2161 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2221 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2281 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2341 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2401 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2461 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2521 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2581 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2641 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2701 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2761 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2821 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2881 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2941 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3001 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3061 ctcttccttt ttcaattcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3121 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     3181 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     3241 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     3301 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     3361 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     3421 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     3481 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     3541 cggcaggagc aaggtgggat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     3601 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     3661 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     3721 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     3781 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     3841 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     3901 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     3961 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     4021 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     4081 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     4141 agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc
     4201 ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4261 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4321 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4381 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4441 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4501 ttgacgggga aagccggcga acgtggcgag
//
