LOCUS       pNEU-KlacRS_2321        8050 bp DNA     circular SYN 17-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(38..43)
                     /locus_tag="termination sequence"
                     /label=termination sequence
     misc_feature    complement(44..112)
                     /locus_tag="M15(2)"
                     /label=M15
                     /label=M15(2)
     promoter        complement(113..370)
                     /locus_tag="Human U6 promoter"
                     /label=Human U6 promoter
     misc_feature    complement(377..382)
                     /locus_tag="termination sequence(2)"
                     /label=termination sequence
                     /label=termination sequence(2)
     misc_feature    complement(383..451)
                     /locus_tag="M15"
                     /label=M15
     promoter        complement(452..709)
                     /locus_tag="Human U6 promoter(2)"
                     /label=Human U6 promoter
                     /label=Human U6 promoter(2)
     misc_feature    complement(716..721)
                     /locus_tag="termination sequence(1)"
                     /label=termination sequence
                     /label=termination sequence(1)
     misc_feature    complement(722..790)
                     /locus_tag="M15(3)"
                     /label=M15
                     /label=M15(3)
     promoter        complement(791..1048)
                     /locus_tag="Human U6 promoter(1)"
                     /label=Human U6 promoter
                     /label=Human U6 promoter(1)
     misc_feature    complement(1055..1060)
                     /locus_tag="termination sequence(3)"
                     /label=termination sequence
                     /label=termination sequence(3)
     misc_feature    complement(1061..1129)
                     /locus_tag="M15(1)"
                     /label=M15
                     /label=M15(1)
     promoter        complement(1130..1387)
                     /locus_tag="Human U6 promoter(3)"
                     /label=Human U6 promoter
                     /label=Human U6 promoter(3)
     primer_bind     complement(1457..1476)
                     /locus_tag="bla-103_rev"
                     /label=bla-103_rev
     promoter        1614..2202
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
     primer_bind     2151..2171
                     /locus_tag="Primer CMV fwd"
                     /label=Primer CMV fwd
     promoter        2245..2264
                     /locus_tag="T7 promoter/priming site"
                     /label=T7 promoter/priming site
     misc_feature    2289..3653
                     /locus_tag="MmAcKRS"
                     /label=MmKlacRS1
                     /label=MmAcKRS
     misc_feature    3189..3191
                     /locus_tag="L301M"
                     /label=L301M
     misc_feature    3204..3206
                     /locus_tag="Y306L"
                     /label=Y306L
     misc_feature    3213..3215
                     /locus_tag="L309A"
                     /label=L309A
     misc_feature    3215
                     /locus_tag="L309"
                     /label=L309
     misc_feature    3330..3332
                     /locus_tag="C348F"
                     /label=C348F
     primer_bind     3680..3697
                     /locus_tag="BGH rev pri"
                     /label=BGH rev pri
     polyA_signal    3686..3910
                     /locus_tag="BGH polyA sequence"
                     /label=BGH polyA sequence
     misc_feature    3753..3773
                     /locus_tag="pNEU-Reverse End seq by Jun Liu"
                     /label=pNEU-Reverse End seq by Jun Liu
     rep_origin      3956..4384
                     /locus_tag="f1 origin"
                     /label=f1 origin
     promoter        4389..4732
                     /locus_tag="SV40 early promoter and origin"
                     /label=SV40 early promoter and origin
     primer_bind     4562..4581
                     /locus_tag="Primer SV40 fwd"
                     /label=Primer SV40 fwd
     gene            4794..5588
                     /locus_tag="Neo resistance gene (ORF)"
                     /label=Neo resistance gene (ORF)
     polyA_signal    5762..5892
                     /locus_tag="SV40 early polyA"
                     /label=SV40 early polyA
     primer_bind     5930..5946
                     /locus_tag="Primer M13r"
                     /label=Primer M13r
     primer_bind     5978..5997
                     /locus_tag="Primer pUCM13-rev-157"
                     /label=Primer pUCM13-rev-157
     rep_origin      complement(6273..6943)
                     /direction=LEFT
                     /locus_tag="pUC origin"
                     /label=pUC origin
     gene            complement(7088..7953)
                     /locus_tag="Amp resistance (ORF)"
                     /label=Amp resistance (ORF)
     primer_bind     7884..7903
                     /locus_tag="Amp Start primer"
                     /label=Amp Start primer
     promoter        complement(7949..8047)
                     /locus_tag="bla promoter"
                     /label=bla promoter
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctga cgtcgacaaa aaacggaaac cccgggaatc
       61 gaacccggct gaacggattt agagtccgtt cggtctccct gaccaggttt ccggtgtttc
      121 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
      181 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
      241 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
      301 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
      361 cttcctgccc ctcgacaaaa aacggaaacc ccgggaatcg aacccggctg aacggattta
      421 gagtccgttc ggtctccctg accaggtttc cggtgtttcg tcctttccac aagatatata
      481 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      541 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      601 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      661 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccc tcgacaaaaa
      721 acggaaaccc cgggaatcga acccggctga acggatttag agtccgttcg gtctccctga
      781 ccaggtttcc ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
      841 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
      901 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
      961 tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa
     1021 tcatgggaaa taggccctct tcctgcccct cgacaaaaaa cggaaacccc gggaatcgaa
     1081 cccggctgaa cggatttaga gtccgttcgg tctccctgac caggtttccg gtgtttcgtc
     1141 ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat
     1201 gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc
     1261 tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct
     1321 ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt
     1381 cctgcccctc gagtctagac ccgatcccct atggtgcact ctcagtacaa tctgctctga
     1441 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     1501 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     1561 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     1621 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1681 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1741 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1801 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1861 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1921 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1981 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2041 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2101 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     2161 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     2221 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     2281 gcgccaccat ggataaaaag cctttgaaca ctctgatttc tgcgaccggt ctgtggatgt
     2341 cccgcaccgg caccatccac aaaatcaaac accatgaagt tagccgttcc aaaatctaca
     2401 ttgaaatggc ttgcggcgat cacctggttg tcaacaactc ccgttcttct cgtaccgctc
     2461 gcgcactgcg ccaccacaaa tatcgcaaaa cctgcaaacg ttgccgtgtt agcgatgagg
     2521 acctgaacaa attcctgacc aaagctaacg aggatcagac ctccgtaaaa gtgaaggtag
     2581 taagcgctcc gacccgtact aaaaaggcta tgccaaaaag cgtggcccgt gccccgaaac
     2641 ctctggaaaa caccgaggcg gctcaggctc aaccatccgg ttctaaattt tctccggcga
     2701 tcccagtgtc cacccaagaa tctgtttccg taccagcaag cgtgtctacc agcattagca
     2761 gcatttctac cggtgctacc gcttctgcgc tggtaaaagg taacactaac ccgattacta
     2821 gcatgtctgc accggtacag gcaagcgccc cagctctgac taaatcccag acggaccgtc
     2881 tggaggtgct gctgaaccca aaggatgaaa tctctctgaa cagcggcaag cctttccgtg
     2941 agctggaaag cgagctgctg tctcgtcgta aaaaggatct gcaacagatc tacgctgagg
     3001 aacgcgagaa ctatctgggt aagctggagc gcgaaattac tcgcttcttc gtggatcgcg
     3061 gtttcctgga gatcaaatct ccgattctga ttccgctgga atacattgaa cgtatgggca
     3121 tcgataatga taccgaactg tctaaacaga tcttccgtgt ggataaaaac ttctgtctgc
     3181 gtccgatgat ggccccgaac ctgctgaact atgcgcgtaa actggaccgt gccctgccgg
     3241 acccgatcaa aattttcgag atcggtcctt gctaccgtaa agagtccgac ggtaaagagc
     3301 acctggaaga attcaccatg ctgaacttct ttcagatggg tagcggttgc acgcgtgaaa
     3361 acctggaatc cattatcacc gacttcctga atcacctggg tatcgatttc aaaattgttg
     3421 gtgacagctg tatggtgtat ggcgatacgc tggatgttat gcacggcgat ctggagctgt
     3481 cttccgcagt agtgggccca atcccgctgg atcgtgagtg gggtatcgac aaaccttgga
     3541 tcggtgcggg ttttggtctg gagcgtctgc tgaaagtaaa acacgacttc aagaacatca
     3601 aacgtgctgc acgttccgag tcctattaca atggtatttc tactaacctg taagcggccg
     3661 ctttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     3721 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     3781 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     3841 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     3901 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     3961 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4021 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4081 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4141 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4201 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4261 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4321 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4381 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4441 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4501 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4561 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     4681 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4741 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     4801 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4861 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4921 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     4981 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5041 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5101 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5161 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5221 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5281 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5341 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5401 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5461 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5521 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5581 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5641 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5701 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5761 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tgtataccgg tcctctagct agagcttggc gtaatcatgg tcatagctgt
     5941 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6001 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6061 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6121 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6181 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6241 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6301 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6361 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6421 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6481 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6541 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6601 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6661 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6721 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6781 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6841 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     6901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7081 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7141 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7201 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7261 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7321 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7381 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7441 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7501 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7561 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7621 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7681 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7741 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7801 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7861 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7921 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7981 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8041 taggggttcc
//
