LOCUS       Exported               15419 bp ds-DNA     circular SYN 11-DEC-2024
DEFINITION  11109169981_V1739_Tc270_pFdelta6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 17, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             278..2698
                     /codon_start=1
                     /label=L4
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MESVEKKDSLTAPSEFATTASTDAANAPTTFPVEAPPLEEEEVII
                     EQDPGFVSEDDEDRSVPTEDKKQDQDNAEANEEQVGRGDERHGDYLDVGDDVLLKHLQR
                     QCAIICDALQERSDVPLAIADVSLAYERHLFSPRVPPKRQENGTCEPNPRLNFYPVFAV
                     PEVLATYHIFFQNCKIPLSCRANRSRADKQLALRQGAVIPDIASLNEVPKIFEGLGRDE
                     KRAANALQQENSENESHSGVLVELEGDNARLAVLKRSIEVTHFAYPALNLPPKVMSTVM
                     SELIVRRAQPLERDANLQEQTEEGLPAVGDEQLARWLQTREPADLEERRKLMMAAVLVT
                     VELECMQRFFADPEMQRKLEETLHYTFRQGYVRQACKISNVELCNLVSYLGILHENRLG
                     QNVLHSTLKGEARRDYVRDCVYLFLCYTWQTAMGVWQQCLEECNLKELQKLLKQNLKDL
                     WTAFNERSVAAHLADIIFPERLLKTLQQGLPDFTSQSMLQNFRNFILERSGILPATCCA
                     LPSDFVPIKYRECPPPLWGHCYLLQLANYLAYHSDIMEDVSGDGLLECHCRCNLCTPHR
                     SLVCNSQLLNESQIIGTFELQGPSPDEKSAAPGLKLTPGLWTSAYLRKFVPEDYHAHEI
                     RFYEDQSRPPNAELTACVITQGHILGQLQAINKARQEFLLRKGRGVYLDPQSGEELNPI
                     PPPPQPYQQQPRALASQDGTQKEAAAAAATHGRGGILGQSGRGGFGRGGGGHDGRLGEP
                     RRGSFRGRRGVRRNTVTLGRIPLAGAPEIGNRFQHGYNLRSSGAAGTARSPTQP"
     CDS             2911..3294
                     /codon_start=1
                     /label=SF33K_ADE02 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=93.8"
                     /note="/match_length=56.1"
                     /note="/other=CDS"
                     /translation="TTVISTAHTAPAAAAATAAATQKQRRPDSKTLTKPKKSTAAAAAG
                     GGALRLAPNEPVSTRELRNRIFPTLYAIFQQSRGQEQELKIKNRSLRSLTRSCLYHKSE
                     DQLRRTLEDAEALFSKYCALTLKD"
     CDS             3385..4065
                     /codon_start=1
                     /label=L4
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSKEIPTPYMWSYQPQMGLAAGAAQDYSTRINYMSAGPHMISRVN
                     GIRAHRNRILLEQAAITTTPRNNLNPRSWPAALVYQESPAPTTVVLPRDAQAEVQMTNS
                     GAQLAGGFRHRVRSPGQGITHLTIRGRGIQLNDESVSSSLGLRPDGTFQIGGAGRSSFT
                     PRQAILTLQTSSSEPRSGGIGTLQFIEEFVPSVYFNPFSGPPGHYPDQFIPNFDAVKDS
                     ADGYD"
     CDS             4069..4347
                     /codon_start=1
                     /label=E312_ADE02 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=87.1"
                     /note="/match_length=86.9"
                     /note="/other=CDS"
                     /translation="MLSGEAEQLRLKHLVHCRRHKCFARDSGEFCYFELPEDHIEGPAH
                     GVRLTAQGELARSLIREFTQRPLLVERDRGPCVLTVICNCPNPGLHQD"
     CDS             complement(4425..4589)
                     /codon_start=1
                     /label=UXP_ADE05 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=25.3"
                     /note="/other=CDS"
                     /translation="MKIVGADGQEQEETDIPFRLWRKFAARRKLQYQSWEEGKEVLLNK
                     LDRNLLTDFK"
     CDS             4600..6342
                     /codon_start=1
                     /label=SPIKE_ADE05
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPG
                     VLSLRLSEPLVTSNGMLALKMGNGLSLDEAGNLTSQNVTTVSPPLKKTKSNINLEISAP
                     LTVTSEALTVAAAAPLMVAGNTLTMQSQAPLTVHDSKLSIATQGPLTVSEGKLALQTSG
                     PLTTTDSSTLTITASPPLTTATGSLGIDLKEPIYTQNGKLGLKYGAPLHVTDDLNTLTV
                     ATGPGVTINNTSLQTKVTGALGFDSQGNMQLNVAGGLRIDSQNRRLILDVSYPFDAQNQ
                     LNLRLGQGPLFINSAHNLDINYNKGLYLFTASNNSKKLEVNLSTAKGLMFDATAIAINA
                     GDGLEFGSPNAPNTNPLKTKIGHGLEFDSNKAMVPKLGTGLSFDSTGAITVGNKNNDKL
                     TLWTTPAPSPNCRLNAEKDAKLTLVLTKCGSQILATVSVLAVKGSLAPISGTVQSAHLI
                     IRFDENGVLLNNSFLDPEYWNFRNGDLTEGTAYTNAVGFMPNLSAYPKSHGKTAKSNIV
                     SQVYLNGDKTKPVTLTITLNGTQETGDTTPSAYSMSFSWDWSGHNYINEIFATSSYTFS
                     YIAQE"
     CDS             complement(6475..6552)
                     /codon_start=1
                     /label=E417_ADE03
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=73.1"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="FKASRGERTVYSVCWEGGGRLNTRVL"
     CDS             complement(6754..7635)
                     /codon_start=1
                     /label=E434_ADE02
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MTTSGVPFGMTLRPTRSRLSRRTPYSRDRLPPFETETRATILEDH
                     PLLPECNTLTMHNVSYVRGLPCSVGFTLIQEWVVPWDMVLTREELVILRKCMHVCLCCA
                     NIDIMTSMMIHGYESWALHCHCSSPGSLQCIAGGQVLASWFRMVVDGAMFNQRFIWYRE
                     VVNYNMPKEVMFMSSVFMRGRHLIYLRLWYDGHVGSVVPAMSFGYSALHCGILNNIVVL
                     CCSYCADLSEIRVRCCARRTRRLMLRAVRIIAEETTAMLYSCRTERRRQQFIRALLQHH
                     RPILMHDYDSTPM"
     CDS             complement(7913..8260)
                     /codon_start=1
                     /label=E411_ADE05
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MIRCLRLKVEGALEQIFTMAGLNIRDLLRDILRRWRDENYLGMVE
                     GAGMFIEEIHPEGFSLYVHLDVRAVCLLEAIVQHLTNAIICSLAVEFDHATGGERVHLI
                     DLHFEVLDNLLE"
     CDS             complement(8260..8649)
                     /codon_start=1
                     /label=E4RF2_ADE02
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MFERKMVSFSVVVPELTCLYLHEHDYDVLSFLREALPDFLSSTLH
                     FISPPMQQAYIGATLVSIAPSMRVIISVGSFVMVPGGEVAALVRADLHDYVQLALRRDL
                     RDRGIFVNVPLLNLIQVCEEPEFLQS"
     CDS             complement(8700..9083)
                     /codon_start=1
                     /label=E4RF1_ADE02
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MAAAVEALYVVLEREGAILPRQEGFSGVYVFFSPINFVIPPMGAV
                     MLSLRLRVCIPPGYFGRFLALTDVNQPDVFTESYIMTPDMTEELSVVLFNHGDQFFYGH
                     AGMAVVRLMLIRVVFPVVRQASNV"
     repeat_region   9393..9494
                     /label=ITR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.0"
                     /note="/other=repeat_region"
     repeat_region   9393..9477
                     /label=ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=82.5"
                     /note="/other=repeat_region"
     repeat_region   9494..9578
                     /label=ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=82.5"
                     /note="/other=repeat_region"
     CDS             complement(9665..9959)
                     /codon_start=1
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=24.8"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWI"
     promoter        complement(10007..10035)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        10148..10252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10253..11113
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11284..11872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             12055..12147
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    12160..12181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12196..12226
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12230..12252
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     protein_bind    12234..12250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12258..12274
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12295..12313
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    12326..12412
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=80.6"
                     /note="/other=misc_feature"
     primer_bind     12350..12366
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(12403..13155)
                     /codon_start=1
                     /label=PTP (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.6"
                     /note="/match_length=37.4"
                     /note="/other=CDS"
                     /translation="SVNDCARLTGQSVPTMEHFLPLRNIWNRVRDFPRASTTAAGITWM
                     SRYIYGYHRLMLEDLAPGAPATLRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAYSRL
                     RYTELSQPGHQTVNWSVMANCTYTINTGAYHRFVDMDDFQSTLTQVQQAILAERVVADL
                     ALLQPMRGFGVTRMGGRGRHLRPNSAAAAAIDARDAGQEEGEEEVPVERLMQDYYKDLR
                     RCQNEAWGMADRLRIQQAGPKDMVLLSTV"
     ncRNA           13184..13340
                     /label=VA
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.7"
                     /note="/other=ncRNA"
     CDS             complement(13321..13545)
                     /codon_start=1
                     /label=Y121_ADE02 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=86.7"
                     /note="/match_length=62.0"
                     /note="/other=CDS"
                     /translation="GKGHPVYAPSLGPRLEPGVPRLNPWKITLRLQGASHLMLSLSSLT
                     AYAARGQWPKKLAQQPPRLEGSQKEHSPVV"
     CDS             13513..13980
                     /codon_start=1
                     /label=L3 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.4"
                     /note="/match_length=76.5"
                     /note="/other=CDS"
                     /translation="ETGGVHWMAFAWNPHSKTCYLFEPFGFSDQRLKQVYQFEYESLLR
                     RSAIASSPDRCITLEKSTQSVQGPNSAACGLFCCMFLHAFANWPQTPMDHNPTMNLITG
                     VPNSMLNSPQVQPTLRRNQEQLYSFLERHSPYFRSHSAQIRSATSFCHLKNM"
     CDS             complement(join(14082..15419,1..249))
                     /codon_start=1
                     /label=DBP
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MASREEEQRETTPERGRGAARRPPTMEDVSSPSPSPPPPRAPPKK
                     RMRRRIESEDEEDSSQDALVPRTPSPRPSTSAADLAIAPKKKKKRPSPKPERPPSPEVI
                     VDSEEEREDVALQMVGFSNPPVLIKHGKGGKRTVRRLNEDDPVARGMRTQEEEEEPSEA
                     ESEITVMNPLSVPIVSAWEKGMEAARALMDKYHVDNDLKANFKLLPDQVEALAAVCKTW
                     LNEEHRGLQLTFTSNKTFVTMMGRFLQAYLQSFAEVTYKHHEPTGCALWLHRCAEIEGE
                     LKCLHGSIMINKEHVIEMDVTSENGQRALKEQSSKAKIVKNRWGRNVVQISNTDARCCV
                     HDAACPANQFSGKSCGMFFSEGAKAQVAFKQIKAFMQALYPNAQTGHGHLLMPLRCECN
                     SKPGHAPFLGRQLPKLTPFALSNAEDLDADLISDKSVLASVHHPALIVFQCCNPVYRNS
                     RAQGGGPNCDFKISAPDLLNALVMVRSLWSENFTELPRMVVPEFKWSTKHQYRNVSLPV
                     AHSDARQNPFDF"
ORIGIN
        1 cgcaatggcc aaatccgccg ccgaggtcga tggccgcggg ctgggtgtgc gcggcaccag
       61 cgcgtcttgt gatgagtctt cctcgtcctc ggactcgata cgccgcctca tccgcttttt
      121 tgggggcgcc cggggaggcg gcggcgacgg ggacggggac gacacgtcct ccatggttgg
      181 gggacgtcgc gccgcaccgc gtccgcgctc gggggtggtt tcgcgctgct cctcttcccg
      241 actggccatt tccttctcct ataggcagaa aaagatcatg gagtcagtcg agaagaagga
      301 cagcctaacc gccccctctg agttcgccac caccgcctcc accgatgccg ccaacgcgcc
      361 taccaccttc cccgtcgagg cacccccgct tgaggaggag gaagtgatta tcgagcagga
      421 cccaggtttt gtaagcgaag acgacgagga ccgctcagta ccaacagagg ataaaaagca
      481 agaccaggac aacgcagagg caaacgagga acaagtcggg cggggggacg aaaggcatgg
      541 cgactaccta gatgtgggag acgacgtgct gttgaagcat ctgcagcgcc agtgcgccat
      601 tatctgcgac gcgttgcaag agcgcagcga tgtgcccctc gccatagcgg atgtcagcct
      661 tgcctacgaa cgccacctat tctcaccgcg cgtacccccc aaacgccaag aaaacggcac
      721 atgcgagccc aacccgcgcc tcaacttcta ccccgtattt gccgtgccag aggtgcttgc
      781 cacctatcac atctttttcc aaaactgcaa gataccccta tcctgccgtg ccaaccgcag
      841 ccgagcggac aagcagctgg ccttgcggca gggcgctgtc atacctgata tcgcctcgct
      901 caacgaagtg ccaaaaatct ttgagggtct tggacgcgac gagaagcgcg cggcaaacgc
      961 tctgcaacag gaaaacagcg aaaatgaaag tcactctgga gtgttggtgg aactcgaggg
     1021 tgacaacgcg cgcctagccg tactaaaacg cagcatcgag gtcacccact ttgcctaccc
     1081 ggcacttaac ctacccccca aggtcatgag cacagtcatg agtgagctga tcgtgcgccg
     1141 tgcgcagccc ctggagaggg atgcaaattt gcaagaacaa acagaggagg gcctacccgc
     1201 agttggcgac gagcagctag cgcgctggct tcaaacgcgc gagcctgccg acttggagga
     1261 gcgacgcaaa ctaatgatgg ccgcagtgct cgttaccgtg gagcttgagt gcatgcagcg
     1321 gttctttgct gacccggaga tgcagcgcaa gctagaggaa acattgcact acacctttcg
     1381 acagggctac gtacgccagg cctgcaagat ctccaacgtg gagctctgca acctggtctc
     1441 ctaccttgga attttgcacg aaaaccgcct tgggcaaaac gtgcttcatt ccacgctcaa
     1501 gggcgaggcg cgccgcgact acgtccgcga ctgcgtttac ttatttctat gctacacctg
     1561 gcagacggcc atgggcgttt ggcagcagtg cttggaggag tgcaacctca aggagctgca
     1621 gaaactgcta aagcaaaact tgaaggacct atggacggcc ttcaacgagc gctccgtggc
     1681 cgcgcacctg gcggacatca ttttccccga acgcctgctt aaaaccctgc aacagggtct
     1741 gccagacttc accagtcaaa gcatgttgca gaactttagg aactttatcc tagagcgctc
     1801 aggaatcttg cccgccacct gctgtgcact tcctagcgac tttgtgccca ttaagtaccg
     1861 cgaatgccct ccgccgcttt ggggccactg ctaccttctg cagctagcca actaccttgc
     1921 ctaccactct gacataatgg aagacgtgag cggtgacggt ctactggagt gtcactgtcg
     1981 ctgcaaccta tgcaccccgc accgctccct ggtttgcaat tcgcagctgc ttaacgaaag
     2041 tcaaattatc ggtacctttg agctgcaggg tccctcgcct gacgaaaagt ccgcggctcc
     2101 ggggttgaaa ctcactccgg ggctgtggac gtcggcttac cttcgcaaat ttgtacctga
     2161 ggactaccac gcccacgaga ttaggttcta cgaagaccaa tcccgcccgc ctaatgcgga
     2221 gcttaccgcc tgcgtcatta cccagggcca cattcttggc caattgcaag ccatcaacaa
     2281 agcccgccaa gagtttctgc tacgaaaggg acggggggtt tacttggacc cccagtccgg
     2341 cgaggagctc aacccaatcc ccccgccgcc gcagccctat cagcagcagc cgcgggccct
     2401 tgcttcccag gatggcaccc aaaaagaagc tgcagctgcc gccgccaccc acggacgagg
     2461 aggaatactg ggacagtcag gcagaggagg ttttggacga ggaggaggag gacatgatgg
     2521 aagactggga gagcctagac gaggaagctt ccgaggtcga agaggtgtca gacgaaacac
     2581 cgtcaccctc ggtcgcattc ccctcgccgg cgccccagaa atcggcaacc ggttccagca
     2641 tggctacaac ctccgctcct caggcgccgc cggcactgcc cgttcgccga cccaaccgta
     2701 gatgggacac cactggaacc agggccggta agtccaagca gccgccgccg ttagcccaag
     2761 agcaacaaca gcgccaaggc taccgctcat ggcgcgggca caagaacgcc atagttgctt
     2821 gcttgcaaga ctgtgggggc aacatctcct tcgcccgccg ctttcttctc taccatcacg
     2881 gcgtggcctt cccccgtaac atcctgcatt actaccgtca tctctacagc ccatactgca
     2941 ccggcggcag cggcagcaac agcagcggcc acacagaagc aaaggcgacc ggatagcaag
     3001 actctgacaa agcccaagaa atccacagcg gcggcagcag caggaggagg agcgctgcgt
     3061 ctggcgccca acgaacccgt atcgacccgc gagcttagaa acaggatttt tcccactctg
     3121 tatgctatat ttcaacagag caggggccaa gaacaagagc tgaaaataaa aaacaggtct
     3181 ctgcgatccc tcacccgcag ctgcctgtat cacaaaagcg aagatcagct tcggcgcacg
     3241 ctggaagacg cggaggctct cttcagtaaa tactgcgcgc tgactcttaa ggactagttt
     3301 cgcgcccttt ctcaaattta agcgcgaaaa ctacgtcatc tccagcggcc acacccggcg
     3361 ccagcacctg ttgtcagcgc cattatgagc aaggaaattc ccacgcccta catgtggagt
     3421 taccagccac aaatgggact tgcggctgga gctgcccaag actactcaac ccgaataaac
     3481 tacatgagcg cgggacccca catgatatcc cgggtcaacg gaatacgcgc ccaccgaaac
     3541 cgaattctcc tggaacaggc ggctattacc accacacctc gtaataacct taatccccgt
     3601 agttggcccg ctgccctggt gtaccaggaa agtcccgctc ccaccactgt ggtacttccc
     3661 agagacgccc aggccgaagt tcagatgact aactcagggg cgcagcttgc gggcggcttt
     3721 cgtcacaggg tgcggtcgcc cgggcagggt ataactcacc tgacaatcag agggcgaggt
     3781 attcagctca acgacgagtc ggtgagctcc tcgcttggtc tccgtccgga cgggacattt
     3841 cagatcggcg gcgccggccg ctcttcattc acgcctcgtc aggcaatcct aactctgcag
     3901 acctcgtcct ctgagccgcg ctctggaggc attggaactc tgcaatttat tgaggagttt
     3961 gtgccatcgg tctactttaa ccccttctcg ggacctcccg gccactatcc ggatcaattt
     4021 attcctaact ttgacgcggt aaaggactcg gcggacggct acgactgaat gttaagtgga
     4081 gaggcagagc aactgcgcct gaaacacctg gtccactgtc gccgccacaa gtgctttgcc
     4141 cgcgactccg gtgagttttg ctactttgaa ttgcccgagg atcatatcga gggcccggcg
     4201 cacggcgtcc ggcttaccgc ccagggagag cttgcccgta gcctgattcg ggagtttacc
     4261 cagcgccccc tgctagttga gcgggacagg ggaccctgtg ttctcactgt gatttgcaac
     4321 tgtcctaacc ctggattaca tcaagatcct ctagttaatt aactagagta cccggggatc
     4381 ttattccctt taactaataa aaaaaaataa taaagcatca cttacttaaa atcagttagc
     4441 aaatttctgt ccagtttatt cagcagcacc tccttgccct cctcccagct ctggtattgc
     4501 agcttcctcc tggctgcaaa ctttctccac aatctaaatg gaatgtcagt ttcctcctgt
     4561 tcctgtccat ccgcacccac tatcttcatg ttgttgcaga tgaagcgcgc aagaccgtct
     4621 gaagatacct tcaaccccgt gtatccatat gacacggaaa ccggtcctcc aactgtgcct
     4681 tttcttactc ctccctttgt atcccccaat gggtttcaag agagtccccc tggggtactc
     4741 tctttgcgcc tatccgaacc tctagttacc tccaatggca tgcttgcgct caaaatgggc
     4801 aacggcctct ctctggacga ggccggcaac cttacctccc aaaatgtaac cactgtgagc
     4861 ccacctctca aaaaaaccaa gtcaaacata aacctggaaa tatctgcacc cctcacagtt
     4921 acctcagaag ccctaactgt ggctgccgcc gcacctctaa tggtcgcggg caacacactc
     4981 accatgcaat cacaggcccc gctaaccgtg cacgactcca aacttagcat tgccacccaa
     5041 ggacccctca cagtgtcaga aggaaagcta gccctgcaaa catcaggccc cctcaccacc
     5101 accgatagca gtacccttac tatcactgcc tcaccccctc taactactgc cactggtagc
     5161 ttgggcattg acttgaaaga gcccatttat acacaaaatg gaaaactagg actaaagtac
     5221 ggggctcctt tgcatgtaac agacgaccta aacactttga ccgtagcaac tggtccaggt
     5281 gtgactatta ataatacttc cttgcaaact aaagttactg gagccttggg ttttgattca
     5341 caaggcaata tgcaacttaa tgtagcagga ggactaagga ttgattctca aaacagacgc
     5401 cttatacttg atgttagtta tccgtttgat gctcaaaacc aactaaatct aagactagga
     5461 cagggccctc tttttataaa ctcagcccac aacttggata ttaactacaa caaaggcctt
     5521 tacttgttta cagcttcaaa caattccaaa aagcttgagg ttaacctaag cactgccaag
     5581 gggttgatgt ttgacgctac agccatagcc attaatgcag gagatgggct tgaatttggt
     5641 tcacctaatg caccaaacac aaatcccctc aaaacaaaaa ttggccatgg cctagaattt
     5701 gattcaaaca aggctatggt tcctaaacta ggaactggcc ttagttttga cagcacaggt
     5761 gccattacag taggaaacaa aaataatgat aagctaactt tgtggaccac accagctcca
     5821 tctcctaact gtagactaaa tgcagagaaa gatgctaaac tcactttggt cttaacaaaa
     5881 tgtggcagtc aaatacttgc tacagtttca gttttggctg ttaaaggcag tttggctcca
     5941 atatctggaa cagttcaaag tgctcatctt attataagat ttgacgaaaa tggagtgcta
     6001 ctaaacaatt ccttcctgga cccagaatat tggaacttta gaaatggaga tcttactgaa
     6061 ggcacagcct atacaaacgc tgttggattt atgcctaacc tatcagctta tccaaaatct
     6121 cacggtaaaa ctgccaaaag taacattgtc agtcaagttt acttaaacgg agacaaaact
     6181 aaacctgtaa cactaaccat tacactaaac ggtacacagg aaacaggaga cacaactcca
     6241 agtgcatact ctatgtcatt ttcatgggac tggtctggcc acaactacat taatgaaata
     6301 tttgccacat cctcttacac tttttcatac attgcccaag aataaagaat cgtttgtgtt
     6361 atgtttcaac gtgtttattt ttcaattgca gaaaatttca agtcattttt cattcagtag
     6421 tatagcccca ccaccacata gcttatacag atcaccgtac cttaatcaaa ctcacagaac
     6481 cctagtattc aacctgccac ctccctccca acacacagag tacacagtcc tttctccccg
     6541 gctggcctta aaaagcatca tatcatgggt aacagacata ttcttaggtg ttatattcca
     6601 cacggtttcc tgtcgagcca aacgctcatc agtgatatta ataaactccc cgggcagctc
     6661 acttaagttc atgtcgctgt ccagctgctg agccacaggc tgctgtccaa cttgcggttg
     6721 cttaacgggc ggcgaaggag aagtccacgc ctacatgggg gtagagtcat aatcgtgcat
     6781 caggataggg cggtggtgct gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt
     6841 cctgcaggaa tacaacatgg cagtggtctc ctcagcgatg attcgcaccg cccgcagcat
     6901 aaggcgcctt gtcctccggg cacagcagcg caccctgatc tcacttaaat cagcacagta
     6961 actgcagcac agcaccacaa tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa
     7021 gctcatggcg gggaccacag aacccacgtg gccatcatac cacaagcgca ggtagattaa
     7081 gtggcgaccc ctcataaaca cgctggacat aaacattacc tcttttggca tgttgtaatt
     7141 caccacctcc cggtaccata taaacctctg attaaacatg gcgccatcca ccaccatcct
     7201 aaaccagctg gccaaaacct gcccgccggc tatacactgc agggaaccgg gactggaaca
     7261 atgacagtgg agagcccagg actcgtaacc atggatcatc atgctcgtca tgatatcaat
     7321 gttggcacaa cacaggcaca cgtgcataca cttcctcagg attacaagct cctcccgcgt
     7381 tagaaccata tcccagggaa caacccattc ctgaatcagc gtaaatccca cactgcaggg
     7441 aagacctcgc acgtaactca cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg
     7501 atgatcctcc agtatggtag cgcgggtttc tgtctcaaaa ggaggtagac gatccctact
     7561 gtacggagtg cgccgagaca accgagatcg tgttggtcgt agtgtcatgc caaatggaac
     7621 gccggacgta gtcatatttc ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc
     7681 gtctccggtc tcgccgctta gatcgctctg tgtagtagtt gtagtatatc cactctctca
     7741 aagcatccag gcgccccctg gcttcgggtt ctatgtaaac tccttcatgc gccgctgccc
     7801 tgataacatc caccaccgca gaataagcca cacccagcca acctacacat tcgttctgcg
     7861 agtcacacac gggaggagcg ggaagagctg gaagaaccat gttttttttt tattccaaaa
     7921 gattatccaa aacctcaaaa tgaagatcta ttaagtgaac gcgctcccct ccggtggcgt
     7981 ggtcaaactc tacagccaaa gaacagataa tggcatttgt aagatgttgc acaatggctt
     8041 ccaaaaggca aacggccctc acgtccaagt ggacgtaaag gctaaaccct tcagggtgaa
     8101 tctcctctat aaacattcca gcaccttcaa ccatgcccaa ataattctca tctcgccacc
     8161 ttctcaatat atctctaagc aaatcccgaa tattaagtcc ggccattgta aaaatctgct
     8221 ccagagcgcc ctccaccttc agcctcaagc agcgaatcat gattgcaaaa attcaggttc
     8281 ctcacagacc tgtataagat tcaaaagcgg aacattaaca aaaataccgc gatcccgtag
     8341 gtcccttcgc agggccagct gaacataatc gtgcaggtct gcacggacca gcgcggccac
     8401 ttccccgcca ggaaccatga caaaagaacc cacactgatt atgacacgca tactcggagc
     8461 tatgctaacc agcgtagccc cgatgtaagc ttgttgcatg ggcggcgata taaaatgcaa
     8521 ggtgctgctc aaaaaatcag gcaaagcctc gcgcaaaaaa gaaagcacat cgtagtcatg
     8581 ctcatgcaga taaaggcagg taagctccgg aaccaccaca gaaaaagaca ccatttttct
     8641 ctcaaacatg tctgcgggtt tctgcataaa cacaaaataa aataacaaaa aaacatttaa
     8701 acattagaag cctgtcttac aacaggaaaa acaaccctta taagcataag acggactacg
     8761 gccatgccgg cgtgaccgta aaaaaactgg tcaccgtgat taaaaagcac caccgacagc
     8821 tcctcggtca tgtccggagt cataatgtaa gactcggtaa acacatcagg ttgattcaca
     8881 tcggtcagtg ctaaaaagcg accgaaatag cccgggggaa tacatacccg caggcgtaga
     8941 gacaacatta cagcccccat aggaggtata acaaaattaa taggagagaa aaacacataa
     9001 acacctgaaa aaccctcctg cctaggcaaa atagcaccct cccgctccag aacaacatac
     9061 agcgcttcca cagcggcagc cataacagtc agccttacca gtaaaaaaga aaacctatta
     9121 aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa agggccaagt
     9181 gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac
     9241 ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc acaacttcct
     9301 caaatcgtca cttccgtttt cccacgttac gtcacttccc attttaagaa aactacaatt
     9361 cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt tcccacgccc
     9421 cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc aaaataaggt
     9481 atattattga tgattatttt ggattgaagc caatatgata atgagggggt ggagtttgtg
     9541 acgtggcgcg gggcgtggga acggggcggg tgacgtagta gtgtggcgga agtgtgatgt
     9601 tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca aaagtgacgt ttttggtgtg
     9661 cgccggatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt
     9721 agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt
     9781 gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg
     9841 ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg
     9901 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata
     9961 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg
    10021 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt
    10081 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
    10141 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    10201 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    10261 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    10321 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    10381 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10441 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    10501 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10561 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10621 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10681 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10741 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10801 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10861 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10921 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10981 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    11041 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    11101 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    11161 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgacccaaat
    11221 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    11281 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    11341 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    11401 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    11461 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    11521 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11581 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11641 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    11701 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    11761 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    11821 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    11881 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    11941 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    12001 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    12061 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    12121 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    12181 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    12241 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    12301 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagaactag
    12361 tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg acagaagcac
    12421 catgtccttg ggtccggcct gctgaatgcg caggcggtcg gccatgcccc aggcttcgtt
    12481 ttgacatcgg cgcaggtctt tgtagtagtc ttgcatgagc ctttctaccg gcacttcttc
    12541 ttctccttcc tcttgtcctg catctcttgc atctatcgct gcggcggcgg cggagtttgg
    12601 ccgtaggtgg cgccctcttc ctcccatgcg tgtgaccccg aagcccctca tcggctgaag
    12661 cagggctagg tcggcgacaa cgcgctcggc taatatggcc tgctgcacct gcgtgagggt
    12721 agactggaag tcatccatgt ccacaaagcg gtggtatgcg cccgtgttga tggtgtaagt
    12781 gcagttggcc ataacggacc agttaacggt ctggtgaccc ggctgcgaga gctcggtgta
    12841 cctgagacgc gagtaagccc tcgagtcaaa tacgtagtcg ttgcaagtcc gcaccaggta
    12901 ctggtatccc accaaaaagt gcggcggcgg ctggcggtag aggggccagc gtagggtggc
    12961 cggggctccg ggggcgagat cttccaacat aaggcgatga tatccgtaga tgtacctgga
    13021 catccaggtg atgccggcgg cggtggtgga ggcgcgcgga aagtcgcgga cgcggttcca
    13081 gatgttgcgc agcggcaaaa agtgctccat ggtcgggacg ctctggccgg tcaggcgcgc
    13141 gcaatcgttg acgctctagc gtgcaaaagg agagcctgta agcgggcact cttccgtggt
    13201 ctggtggata aattcgcaag ggtatcatgg cggacgaccg gggttcgagc cccgtatccg
    13261 gccgtccgcc gtgatccatg cggttaccgc ccgcgtgtcg aacccaggtg tgcgacgtca
    13321 gacaacgggg gagtgctcct tttggcttcc ttccaggcgc ggcggctgct gcgctagctt
    13381 ttttggccac tggccgcgcg cagcgtaagc ggttaggctg gaaagcgaaa gcattaagtg
    13441 gctcgctccc tgtagccgga gggttatttt ccaagggttg agtcgcggga cccccggttc
    13501 gagtctcgga ccgagactgg gggcgtacac tggatggcct ttgcctggaa cccgcactca
    13561 aaaacatgct acctctttga gccctttggc ttttctgacc agcgactcaa gcaggtttac
    13621 cagtttgagt acgagtcact cctgcgccgt agcgccattg cttcttcccc cgaccgctgt
    13681 ataacgctgg aaaagtccac ccaaagcgta caggggccca actcggccgc ctgtggacta
    13741 ttctgctgca tgtttctcca cgcctttgcc aactggcccc aaactcccat ggatcacaac
    13801 cccaccatga accttattac cggggtaccc aactccatgc tcaacagtcc ccaggtacag
    13861 cccaccctgc gtcgcaacca ggaacagctc tacagcttcc tggagcgcca ctcgccctac
    13921 ttccgcagcc acagtgcgca gattaggagc gccacttctt tttgtcactt gaaaaacatg
    13981 taaaaataat gtactagaga cactttcaat aaaggcaaat gcttttattt gtacactctc
    14041 gggtgattat ttacccccac ccttgccgtc tgcgccgttt aaaaatcaaa ggggttctgc
    14101 cgcgcatcgc tatgcgccac tggcagggac acgttgcgat actggtgttt agtgctccac
    14161 ttaaactcag gcacaaccat ccgcggcagc tcggtgaagt tttcactcca caggctgcgc
    14221 accatcacca acgcgtttag caggtcgggc gccgatatct tgaagtcgca gttggggcct
    14281 ccgccctgcg cgcgcgagtt gcgatacaca gggttgcagc actggaacac tatcagcgcc
    14341 gggtggtgca cgctggccag cacgctcttg tcggagatca gatccgcgtc caggtcctcc
    14401 gcgttgctca gggcgaacgg agtcaacttt ggtagctgcc ttcccaaaaa gggcgcgtgc
    14461 ccaggctttg agttgcactc gcaccgtagt ggcatcaaaa ggtgaccgtg cccggtctgg
    14521 gcgttaggat acagcgcctg cataaaagcc ttgatctgct taaaagccac ctgagccttt
    14581 gcgccttcag agaagaacat gccgcaagac ttgccggaaa actgattggc cggacaggcc
    14641 gcgtcgtgca cgcagcacct tgcgtcggtg ttggagatct gcaccacatt tcggccccac
    14701 cggttcttca cgatcttggc cttgctagac tgctccttca gcgcgcgctg cccgttttcg
    14761 ctcgtcacat ccatttcaat cacgtgctcc ttatttatca taatgcttcc gtgtagacac
    14821 ttaagctcgc cttcgatctc agcgcagcgg tgcagccaca acgcgcagcc cgtgggctcg
    14881 tgatgcttgt aggtcacctc tgcaaacgac tgcaggtacg cctgcaggaa tcgccccatc
    14941 atcgtcacaa aggtcttgtt gctggtgaag gtcagctgca acccgcggtg ctcctcgttc
    15001 agccaggtct tgcatacggc cgccagagct tccacttggt caggcagtag tttgaagttc
    15061 gcctttagat cgttatccac gtggtacttg tccatcagcg cgcgcgcagc ctccatgccc
    15121 ttctcccacg cagacacgat cggcacactc agcgggttca tcaccgtaat ttcactttcc
    15181 gcttcgctgg gctcttcctc ttcctcttgc gtccgcatac cacgcgccac tgggtcgtct
    15241 tcattcagcc gccgcactgt gcgcttacct cctttgccat gcttgattag caccggtggg
    15301 ttgctgaaac ccaccatttg tagcgccaca tcttctcttt cttcctcgct gtccacgatt
    15361 acctctggtg atggcgggcg ctcgggcttg ggagaagggc gcttcttttt cttcttggg
//
