LOCUS       Exported               11421 bp ds-DNA     circular SYN 11-DEC-2024
DEFINITION  11109169981_V1740_Tc271_PRV1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 17, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(62..490)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(478..783)
                     /codon_start=1
                     /label=M13 gene IV (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=23.9"
                     /note="/other=CDS"
                     /translation="VAMAGGNIVLDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQ
                     TLLLGGLTDYKNTSQDSGVPFLSKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL"
     CDS             complement(794..861)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.1"
                     /note="/other=CDS"
                     /translation="AGVIAKRPAPIALPNSCAA*MA"
     repeat_region   866..968
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             complement(1066..3216)
                     /codon_start=1
                     /label=VP1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=98.1"
                     /note="/match_length=97.6"
                     /note="/other=CDS"
                     /translation="KPGPFANHVHTSYLPPTERHKDDSRGLVLPGYKYLGPFNGLDKGE
                     PVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQAK
                     KRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADS
                     VPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVI
                     TTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRL
                     INNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAH
                     QGCLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFED
                     VPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRN
                     WLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFP
                     QSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATAD
                     VNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPV
                     PANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDF
                     TVDTNGVYSEPRPIGTRYLTRNL"
     CDS             complement(1210..1221)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          complement(3165..3556)
                     /label=beta-globin intron (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=82.4"
                     /note="/other=intron"
     intron          complement(3924..4012)
                     /label=beta-globin intron (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.9"
                     /note="/match_length=18.7"
                     /note="/other=intron"
     CDS             complement(4014..4118)
                     /codon_start=1
                     /label=VP1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.8"
                     /note="/other=CDS"
                     /translation="MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPA"
     CDS             complement(4138..5328)
                     /codon_start=1
                     /label=Rep52
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDN
                     AGKIMSLTKTAPDYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRN
                     TIWLFGPATTGKTNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVV
                     ESAKAILGGSKVRVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMF
                     KFELTRRLDHDFGKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEP
                     KRVRESVAQPSTSDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHG
                     QKDCLECFPVSESQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
     intron          complement(5668..6059)
                     /label=beta-globin intron (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=82.4"
                     /note="/other=intron"
     CDS             complement(6343..6698)
                     /codon_start=1
                     /label=lambda repressor (ts) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=49.9"
                     /note="/other=CDS"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPK"
     RBS             6787..6795
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6799..6996
                     /codon_start=1
                     /label=cro
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTIN
                     ADGSVYAEEVKPFPSNKKTTA"
     CDS             7136..7363
                     /codon_start=1
                     /label=C1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=56.6"
                     /note="/match_length=82.6"
                     /note="/other=CDS"
                     /translation="RNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWKRDWIPKF
                     SMLLAVLEWGVVDDDMARLARQVAAILTNKK"
     terminator      complement(7346..7440)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             7444..7929
                     /codon_start=1
                     /label=O (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=48.6"
                     /note="/other=CDS"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGADL
                     TKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQGG
                     MFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSSEN"
     intron          complement(8019..8107)
                     /label=beta-globin intron (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.9"
                     /note="/match_length=18.7"
                     /note="/other=intron"
     CDS             complement(8105..8440)
                     /codon_start=1
                     /label=Rep68 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.2"
                     /note="/match_length=20.9"
                     /note="/other=CDS"
                     /translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
                     LGRFLSQV"
     repeat_region   8582..8684
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             complement(8691..8744)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.0"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQ"
     rep_origin      complement(8927..9515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9686..10546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10547..10651)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10746..10781
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     misc_feature    10883..10982
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=70.9"
                     /note="/other=misc_feature"
     CDS             complement(10990..11393)
                     /codon_start=1
                     /label=M13 gene II (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=32.8"
                     /note="/other=CDS"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVETSQK*LPSPA*IYQL
                     ERLNIILMVI*LSPAFLTRLNLYLHITQALHLKYMRVLKIFILALK*RLLPQKYYRVIM
                     FLVQPI*LYALRLYCLILLILCLACMIYWM"
     rep_origin      complement(join(11398..11421,1..490))
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(11398..11421)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.7"
                     /note="/other=rep_origin"
ORIGIN
        1 cccaaaaaca ggaagattgt ataagcaaat atttaaattg taagcgttaa tattttgtta
       61 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      121 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      181 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      241 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      301 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
      361 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
      421 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
      481 cagggcgcgt actatggttg ctttgacgag cacgtataac gtgctttcct cgttagaatc
      541 agagcgggag ctaaacagga ggccgattaa agggatttta gacaggaacg gtacgccaga
      601 atcctgagaa gtgtttttat aatcagtgag gccaccgagt aaaagagtct gtccatcacg
      661 caaattaacc gttgtcgcaa tacttctttg attagtaata acatcacttg cctgagtaga
      721 agaactcaaa ctatcggcct tgctggtaat atccagaaca atattaccgc cagccattgc
      781 aacggaatcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      841 cctcttcgct attacgccag cgatgcatca tcaataatat accttatttt ggattgaagc
      901 caatatgata atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg
      961 tgacgtaggt ttctctagag catggaaact agataagaaa gaaatacgca gagaccaaag
     1021 ttcaactgaa acgaattaaa cggtttattg attaacaagc aattacagat tacgagtcag
     1081 gtatctggtg ccaatggggc gaggctctga atacacgcca ttagtgtcca cagtaaagtc
     1141 cacattaaca gacttgttgt agttggaagt gtactgaatt tcgggattcc agcgtttgct
     1201 gttttccttc tgcagctccc actcgatctc cacgctgacc tgtcccgtgg agtactgtgt
     1261 gatgaaggaa gcaaactttg ccgcactgaa ggtggtcgaa ggattcgcag gtaccggggt
     1321 gttcttgatg agaatctgtg gaggagggtg tttaagtccg aatccaccca tgaggggaga
     1381 ggggtgaaaa tgtccgtccg tgtgtggaat ctttgcccag atgggcccct gaaggtacac
     1441 atctctgtcc tgccagacca tgcctggaag aacgccttgt gtgttgacat ctgcggtagc
     1501 tgcttgtctg ttgcctctct ggaggttggt agatacagaa ccatactgct ccgtagccac
     1561 gggattggtt gtcctgattt cctcttcgtc tgtaatcatg accttttcaa tgtccacatt
     1621 tgttttctct gagccttgct tcccaaagat gagaaccccg ctctgaggaa aaaacttttc
     1681 ttcatcgtcc ttgtggcttg ccatggccgg gcccggattc accagagagt ctctgccatt
     1741 gaggtggtac ttggtagctc cagtccacga gtattcactg ttgttgttat ccgcagatgt
     1801 ctttgatact cgctgctggc ggtaacaggg tccaggaagc cagttcctag actggtcccg
     1861 aatgtcactc gctccggcct gagaaaactg aagccttgac tgcgtggtgg ttccacttgg
     1921 agtgtttgtt ctgctcaagt aatacaggta ctggtcgatg agaggattca tgagacggtc
     1981 cagactctgg ctgtgagcgt agctgctgtg gaaaggaacg tcctcaaaag tgtagctgaa
     2041 ggtaaagttg tttccggtac gcagcatctg agaaggaaag tactccaggc agtaaaatga
     2101 agagcgtcct actgcctgac tcccgttgtt cagggtgagg tatccatact gtggcaccat
     2161 gaagacgtct gctgggaacg gcgggaggca tccttgatgc gccgagccga ggacgtacgg
     2221 gagctggtac tccgagtcag taaacacctg aaccgtgctg gtaaggttat tggcaatcgt
     2281 cgtcgtaccg tcattctgcg tgacctcttt gacttgaatg ttaaagagct tgaagttgag
     2341 tctcttgggt cggaatcccc agttgttgtt gatgagtctt tgccagtcac gtggtgaaaa
     2401 gtggcagtgg aatctgttga agtcaaaata cccccaaggg gtgctgtagc caaagtagtg
     2461 attgtcgttc gaggctcctg attggctgga aatttgtttg tagaggtggt tgttgtaggt
     2521 gggcagggcc caggttcggg tgctggtggt gatgactctg tcgcccatcc atgtggaatc
     2581 gcaatgccaa tttcccgagg aattacccac tccgtcggcg ccctcgttat tgtctgccat
     2641 tggtgcgcca ctgcctgtag ccatcgtatt agttcccaga ccagaggggg ctgctggtgg
     2701 ctgtccgaga ggctgggggt caggtactga gtctgcgtct ccagtctgac caaaattcaa
     2761 tctttttctt gcaggctgct ggcccgcctt tccggttccc gaggaggagt ctggctccac
     2821 aggagagtgc tctaccggcc tcttttttcc cggagccgtc ttaacaggtt cctcaaccag
     2881 gcccagaggt tcaagaaccc tctttttcgc ctggaagact gctcgtccga ggttgccccc
     2941 aaaagacgta tcttctttaa ggcgctcctg aaactccgcg tcggcgtggt tgtacttgag
     3001 gtacgggttg tctccgctgt cgagctgccg gtcgtaggct ttgtcgtgct cgagggccgc
     3061 ggcgtctgcc tcgttgaccg gctctccctt gtcgagtccg ttgaagggtc cgaggtactt
     3121 gtacccagga agcacaagac ccctgctgtc gtccttatgc cgctctgtgg gaggaagata
     3181 agaggtatga acatgattag caaaagggcc tggcttggac tcagaataat ccagccttat
     3241 cccaaccata aaataaaagc agaatggtag ctggattgta gctgctatta gcaatatgaa
     3301 acctcttaca tcagttacaa tttatatgca gaaatattta tatgcagaaa tattgctatt
     3361 gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagagg catgatacat
     3421 tgtatcatta ttgccctgaa agaaagagat tagggaaagt attagaaata agataaacaa
     3481 aaaagtatat taaaagaaga aagcattttt taaaattaca aatgcaaaat taccctgatt
     3541 tggtcaatat gtgtacacat ataaaacatt acactttaac ccataaatat gtataatgat
     3601 tatgtatcaa ttaaaaataa aagaaaataa agtagggaga ttatgaatat gcaaataagc
     3661 acacatatat tccaaatagt aatgtactag gcagactgtg taaagttttt ttttaagtta
     3721 cttaatgtat ctcagagata tttccttttg ttatacacaa tgttaaggca ttaagtataa
     3781 tagtaaaaat tgcggagaag aaaaaaaaga aagcaagaat taaacaaaag aaaacaattg
     3841 ttatgaacag caaatagaag aaactaaaac gatcctgaga cttccacact gatgcaatca
     3901 ttcgtctgtt tcccattcta aactgtaccc tgttacttat ccccttccta tgacatgaac
     3961 ttaaccatag aaaagaaggg gaaagaaaac atcaagggtc ccatagactc acctgcgggc
     4021 tttggtggtg gtgggccagg tttgagcttc caccactgtc ttattccttc agagagagtg
     4081 tcctcgagcc aatctggaag ataaccatcg gcagccatac ctgatttaaa tcatttattg
     4141 ttcaaagatg cagtcatcca aatccacatt gaccagatcg caggcagtgc aagcgtctgg
     4201 cacctttccc atgatatgat gaatgtagca cagtttctga tacgcctttt tgacgacaga
     4261 aacgggttga gattctgaca cgggaaagca ctctaaacag tctttctgtc cgtgagtgaa
     4321 gcagatattt gaattctgat tcattctctc gcattgtctg cagggaaaca gcatcagatt
     4381 catgcccacg tgacgagaac atttgttttg gtacctgtct gcgtagttga tcgaagcttc
     4441 cgcgtctgac gtcgatggct gcgcaactga ctcgcgcacc cgtttgggct cacttatatc
     4501 tgcgtcactg ggggcgggtc ttttcttggc tccacccttt ttgacgtaga attcatgctc
     4561 cacctcaacc acgtgatcct ttgcccaccg gaaaaagtct ttgacttcct gcttggtgac
     4621 cttcccaaag tcatgatcca gacggcgggt gagttcaaat ttgaacatcc ggtcttgcaa
     4681 cggctgctgg tgttcgaagg tcgttgagtt cccgtcaatc acggcgcaca tgttggtgtt
     4741 ggaggtgacg atcacgggag tcgggtctat ctgggccgag gacttgcatt tctggtccac
     4801 gcgcaccttg cttcctccga gaatggcttt ggccgactcc acgaccttgg cggtcatctt
     4861 cccctcctcc caccagatca ccatcttgtc gacacagtcg ttgaagggaa agttctcatt
     4921 ggtccagttt acgcacccgt agaagggcac agtgtgggct atggcctccg cgatgttggt
     4981 cttcccggta gttgcaggcc caaacagcca gatggtgttc ctcttgccga actttttcgt
     5041 ggcccatccc agaaagacgg aagccgcata ttggggatcg tacccgttta gttccaaaat
     5101 tttataaatc cgattgctgg aaatgtcctc cacgggctgc tggcccacca ggtagtcggg
     5161 ggcggtttta gtcaggctca taatctttcc cgcattgtcc aaggcagcct tgatttggga
     5221 ccgcgagttg gaggccgcat tgaaggagat gtatgaggcc tggtcctcct ggatccactg
     5281 cttctccgag gtaatcccct tgtccacgag ccacccgacc agctccatgt acctggctga
     5341 agtttttgat ctgatcaccg gcgcatcaga attgggattc tgattctctt tgttctgctc
     5401 ctgcgtctgc gacacgtgcg tcagatgctg cgccaccaac cgtttacgct ccgtgagatt
     5461 caaacaggcg cttaaatact gttccatatt agtccacgcc cactggagct caggctgggt
     5521 tttggggagc aagtaattgg ggatgtagca ctcatccacc accttgttcc cgcctccggc
     5581 gccatttctg gtctttgtga ccgcgaacca gtttggcaaa gtcggctcga tcccgcggta
     5641 aattctctga atcagttttt cgcgaatctg tgggaggaag ataagaggta tgaacatgat
     5701 tagcaaaagg gcctggcttg gactcagaat aatccagcct tatcccaacc ataaaataaa
     5761 agcagaatgg tagctggatt gtagctgcta ttagcaatat gaaacctctt acatcagtta
     5821 caatttatat gcagaaatat ttatatgcag aaatattgct attgccttaa cccagaaatt
     5881 atcactgtta ttctttagaa tggtgcaaag aggcatgata cattgtatca ttattgccct
     5941 gaaagaaaga gattagggaa agtattagaa ataagataaa caaaaaagta tattaaaaga
     6001 agaaagcatt ttttaaaatt acaaatgcaa aattaccctg atttggtcaa tatgtgtaca
     6061 catataaaac attacacttt aacccataaa tatgtataat gattatgtat caattaaaaa
     6121 taaaagaaaa taaagtaggg agattatgaa tatgcaaata agcacacata tattccaaat
     6181 agtaatgtac taggcagact gtgtaaagtt tttttttaag ttacttaatg tatctcagag
     6241 atatttcctt ttgttataca caatgttaag gcattaagta taatagtaaa aattgcggag
     6301 aagaaaaaaa agaaagcaag aattaaacaa aagaaaacaa ttagcttagg tgagaacatc
     6361 cctgcctgaa catgagaaaa aacagggtac tcatactcac ttctaagtga cggctgcata
     6421 ctaaccgctt catacatctc gtagatttct ctggcgattg aagggctaaa ttcttcaacg
     6481 ctaactttga gaatttttgt aagcaatgcg gcgttataag catttaatgc attgatgcca
     6541 ttaaataaag caccaacgcc tgactgcccc atccccatct tgtctgcgac agattcctgg
     6601 gataagccaa gttcattttt ctttttttca taaattgctt taaggcgacg tgcgtcctca
     6661 agctgctctt gtgttaatgg tttctttttt gtgctcatac gttaaatcta tcaccgcaag
     6721 ggataaatat ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc
     6781 atgtactaag gaggttgtat ggaacaacgc ataaccctga aagattatgc aatgcgcttt
     6841 gggcaaacca agacagctaa agatctcggc gtatatcaaa gcgcgatcaa caaggccatt
     6901 catgcaggcc gaaagatttt tttaactata aacgctgatg gaagcgttta tgcggaagag
     6961 gtaaagccct tcccgagtaa caaaaaaaca acagcataaa taaccccgct cttacacatt
     7021 ccagccctga aaaagggcat caaattaaac cacacctatg gtgtatgcat ttatttgcat
     7081 acattcaatc aattgttatc taaggaaata cttacatatg gttcgtgcaa acaaacgcaa
     7141 cgaggctcta cgaatcgaga gtgcgttgct taacaaaatc gcaatgcttg gaactgagaa
     7201 gacagcggaa gctgtgggcg ttgataagtc gcagatcagc aggtggaaga gggactggat
     7261 tccaaagttc tcaatgctgc ttgctgttct tgaatggggg gtcgttgacg acgacatggc
     7321 tcgattggcg cgacaagttg ctgcgattct caccaataaa aaacgcccgg cggcaaccga
     7381 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     7441 attatgacaa atacagcaaa aatactcaac ttcggcagag gtaactttgc cggacaggag
     7501 cgtaatgtgg cagatctcga tgatggttac gccagactat caaatatgct gcttgaggct
     7561 tattcgggcg cagatctgac caagcgacag tttaaagtgc tgcttgccat tctgcgtaaa
     7621 acctatgggt ggaataaacc aatggacaga atcaccgatt ctcaacttag cgagattaca
     7681 aagttacctg tcaaacggtg caatgaagcc aagttagaac tcgtcagaat gaatattatc
     7741 aagcagcaag gcggcatgtt tggaccaaat aaaaacatct cagaatggtg catccctcaa
     7801 aacgagggaa aatcccctaa aacgagggat aaaacatccc tcaaattggg ggattgctat
     7861 ccctcaaaac agggggacac aaaagacact attacaaaag aaaaaagaaa agattattcg
     7921 tcagagaatt aattgttatg aacagcaaat aaaagaaact aaaacgatcc tgagacttcc
     7981 acactgatgc aatcattcgt ctgtttccca ttctaaactg taccctgtta cttatcccct
     8041 tcctatgaca tgaacttaac catagaaaag aaggggaaag aaaacatcaa gggtcccata
     8101 gactcacctg actcaggaaa cgtcccaaaa ccatggattt caccccggtg gtttccacga
     8161 gcacgtgcat gtggaagtag ctctctccct tctcaaattg cacaaagaaa agggcctccg
     8221 gggccttact cacacggcgc cattccgtca gaaagtcgcg ctgcagcttc tcggccacgg
     8281 tcaggggtgc ctgctcaatc agattcagat ccatgtcaga atctggcggc aactcccatt
     8341 ccttctcggc cacccagttc acaaagctgt cagaaatgcc gggcagatgc tcgtcaaggt
     8401 cgctggggac cttaatcaca atctcgtaaa accccggcat ggcggctgcg cgttcaaacc
     8461 tcccgcttca aaatggagac cctgcgtgct cactcgggct taaataccca gcgtgaccac
     8521 atggtgtcgc aaaatgtcgc aaaacactca cgtgacctct aatacaggac ctctagagct
     8581 actacgtcac ccgccccgtt cccacgcccc gcgccacgtc acaaactcca ccccctcatt
     8641 atcatattgg cttcaatcca aaataaggta tattattgat gatgggaatt ctgcattaat
     8701 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9361 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9601 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10621 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10681 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10741 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    10801 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10861 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10921 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10981 ggcgattcca acatccaata aatcatacag gcaaggcaaa gaattagcaa aattaagcaa
    11041 taaagcctca gagcataaag ctaaatcggt tgtaccaaaa acattatgac cctgtaatac
    11101 ttttgcggga gaagccttta tttcaacgca aggataaaaa tttttagaac cctcatatat
    11161 tttaaatgca atgcctgagt aatgtgtagg taaagattca aacgggtgag aaaggccgga
    11221 gacagtcaaa tcaccatcaa tatgatattc aaccgttcta gctgataaat tcatgccgga
    11281 gagggtagct atttttgaga ggtctctaca aaggctatca ggtcattgcc tgagagtctg
    11341 gagcaaacaa gagaatcgat gaacggtaat cgtaaaacta gcatgtcaat catatgtacc
    11401 ccggttgata atcagaaaag c
//
