LOCUS       RUBY-ABE#2       16235 bp DNA     circular SYN 22-DEC-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16235)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 22, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16235
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    2301..2306
                     /label=Esp3I recognition site(1)
                     /note="Esp3I recognition site"
     misc_feature    2764
                     /label=G to C
                     /note="To kill BsaI site"
     misc_feature    complement(5150..5155)
                     /label=Esp3I recognition site
                     /note="Esp3I recognition site"
     misc_feature    complement(6234..6259)
                     /label=RightBorder
                     /note="RightBorder"
     misc_feature    complement(6338..6500)
                     /label=LacZpromoter(1)
                     /note="LacZpromoter"
     promoter        6519..7287
                     /label=2X35S promoter
                     /note="2X35S promoter"
     protein_bind    7328..7352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7356..7391
                     /label=Unknown feature
     CDS             7392..7412
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7413..7910
                     /codon_start=1
                     /label=ecTadA8e
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQS
                     SIN"
     misc_feature    7734..7736
                     /label=A109S
     misc_feature    7740..7742
                     /label=T111R
     misc_feature    7764..7766
                     /label=D119N
     misc_feature    7773..7775
                     /label=H122N
     misc_feature    7848..7850
                     /label=Y147D
     misc_feature    7854..7856
                     /label=F149D
     misc_feature    7905..7907
                     /label=T166I
     misc_feature    7908..7910
                     /label=D167N
     misc_feature    7911..8006
                     /label=32 Aa linker
     sig_peptide     8013..8078
                     /label=3xFLAG
     misc_feature    8085..8105
                     /label=SV40 NLS
     CDS             8130..12230
                     /codon_start=1
                     /label=zCas9
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    8154..8156
                     /label=D10A
     misc_feature    12231..12278
                     /label=nucleoplasmin NLS
     terminator      12286..12906
                     /label=rbcS-E9t
                     /note="/dnas_title=rbcS-E9t
                     /vntifkey=43"
     protein_bind    12953..12973
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_feature    12990..13002
                     /label=For Left homology arm
                     /label=nonstandard type: insertion_seq
                     /note="/dnas_title=For Left homology arm"
                     /note="/vntifkey=14"
     promoter        13010..13331
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     modified_base   13085
                     /label=G to c
                     /note="G to c"
     misc_feature    13271..13280
                     /label=USE
                     /note="USE"
     TATA_signal     13305..13312
                     /note="TATA box"
     misc_feature    13338..13358
                     /label=sgRNA
     misc_feature    13359..13434
                     /label=guide RNA scaffold
                     /note="guide RNA scaffold"
     terminator      13435..13616
                     /note="AtU3 terminator"
     protein_bind    complement(13637..13661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(13715..13954)
                     /label=nos terminator
                     /note="nos terminator"
     terminator      complement(13955..13972)
                     /label=nos terminator(1)
                     /note="nos terminator"
     misc_feature    complement(14001..14064)
                     /label=LacZpromoter
                     /note="LacZpromoter"
     misc_binding    complement(14082..14104)
                     /label=LacO
                     /note="LacO"
     misc_feature    complement(14294..15074)
                     /label=2X35S Promoter
                     /note="2X35S Promoter"
     misc_feature    complement(15105..15902)
                     /label=nptII (kanR)
                     /note="nptII"
     misc_feature    complement(15940..16143)
                     /label=CaMV 3'UTR(polyA signal)
                     /note="CaMV 3'UTR"
     misc_feature    complement(16210..16235)
                     /label=LeftBorder
                     /note="LeftBorder"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac
     6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
     6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
     6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag
     7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgatgaa acggacagcc gacggaagcg
     7381 agttcgagtc accaaagaag aagcggaaag tctctgaggt ggagttttcc cacgagtact
     7441 ggatgagaca tgccctgacc ctggccaaga gggcacggga tgagagggag gtgcctgtgg
     7501 gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga gccatcggcc
     7561 tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc ctggtcatgc
     7621 agaactacag actgattgac gccaccctgt acgtgacatt cgagccttgc gtgatgtgcg
     7681 ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg aactcaaaaa
     7741 gaggcgccgc aggctccctg atgaacgtgc tgaactaccc cggcatgaat caccgcgtcg
     7801 aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgcgat ttctatcgga
     7861 tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccatcaac tccggaggat
     7921 ctagcggagg ctcctctggc tctgagacac ctggcacaag cgagagcgca acacctgaaa
     7981 gcagcggggg cagcagcggg gggtcaacca tggattacaa ggaccacgac ggggattaca
     8041 aggaccacga cattgattac aaggatgatg atgacaagat ggctccgaag aagaagagga
     8101 aggttggcat ccacggggtg ccagctgctg acaagaagta ctcgatcggc ctcgctattg
     8161 ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc tcaaagaagt
     8221 tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc
     8281 tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt
     8341 acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac gagatggcga
     8401 aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag gaggataaga
     8461 agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt
     8521 accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag gcggacctcc
     8581 gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc ctgatcgagg
     8641 gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc gtgcagacgt
     8701 acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc
     8761 tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg
     8821 agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc acgcccaact
     8881 tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag gacacatacg
     8941 acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac ctgttcctcg
     9001 ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg aacaccgaga
     9061 ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac catcaggatc
     9121 tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag gagatcttct
     9181 tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct caggaggagt
     9241 tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag ctgctggtga
     9301 agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc agcatcccac
     9361 accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac ttctaccctt
     9421 tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc ccgtactacg
     9481 tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag tcagaggaga
     9541 cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca
     9601 tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc cctaagcact
     9661 cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag tatgtgaccg
     9721 agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc gtggacctcc
     9781 tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac ttcaagaaga
     9841 ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac gcctccctgg
     9901 ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac aacgaggaga
     9961 atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac cgggagatga
    10021 tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg aagcagctca
    10081 agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac ggcattcggg
    10141 acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc gcgaaccgca
    10201 atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc cagaaggctc
    10261 aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcgccag
    10321 ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg aaggtcatgg
    10381 gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag accacgcaga
    10441 agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt aaggagctgg
    10501 ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat gagaagctct
    10561 acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg gatattaaca
    10621 ggctcagcga ttacgacgtc gatcatatcg ttccacagtc attcctgaag gatgactcca
    10681 ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat aatgttcctt
    10741 cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat gccaagctga
    10801 tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc tctgagctgg
    10861 acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact aagcacgttg
    10921 cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag ctgatccgcg
    10981 aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag gatttccagt
    11041 tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac ctgaacgcgg
    11101 tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc gtgtacgggg
    11161 actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag attggcaagg
    11221 ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca gagatcactc
    11281 tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag acgggggaga
    11341 tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc atgccacaag
    11401 tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag tcaattctgc
    11461 ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg aagaagtacg
    11521 gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag gttgagaagg
    11581 gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt atggagcgct
    11641 ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag gaggtgaaga
    11701 aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac ggcaggaagc
    11761 ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg ccgtccaagt
    11821 atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc cccgaggaca
    11881 acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcattgagc
    11941 agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat aaggtcctct
    12001 ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat atcattcatc
    12061 tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac acaactatcg
    12121 atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc caccagtcga
    12181 ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac aagcggccag
    12241 cggcgacgaa gaaggcgggg caggcgaaga agaagaagtg agctcagagc tttcgttcgt
    12301 atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc gcacacacca
    12361 gaatcctact gagtttgagt attatggcat tgggaaaact gtttttcttg taccatttgt
    12421 tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa
    12481 tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat
    12541 ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt
    12601 ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag
    12661 taaaacactt gtagttgtac cattatgctt attcactagg caacaaatat attttcagac
    12721 ctagaaaagc tgcaaatgtt actgaataca agtatgtcct cttgtgtttt agacatttat
    12781 gaactttcct ttatgtaatt ttccagaatc cttgtcagat tctaatcatt gctttataat
    12841 tatagttata ctcatggatt tgtagttgag tatgaaaata ttttttaatg cattttatga
    12901 cttgccaatt gttccggaac tctagataag cttaccggaa agggcgaatt cgcaactttg
    12961 tatacaaaag ttgccccatg gcgttccctc tagataacgc aggatcctat ttactttaaa
    13021 tttttcttat ggctcagcct gtgatggata actgaatcaa acaaatggcg tctgggttta
    13081 agaacatctg ttttggctat gttggacgaa acaagtgaac ttttaggatc aacttccgtt
    13141 tatatacgga gcttatatcg agcaataaga taagtgggct ttttatgtaa tttaatgggc
    13201 tatcgtccat atattcacta atacccatgc ccagtaccca tgtatgcgtt tcatataagc
    13261 tcctaatttc tcccacatcg ctcaaatcta aacaaatctt gttgtatata taacactgag
    13321 ggagcaccat tggtcactct atggggagag cagatgcagt tttagagcta gaaatagcaa
    13381 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
    13441 ttccctttcc ttttttcttt tttttgccat aaacttaaat ttgtatatcg atcattgtag
    13501 atattgaaaa cctagaacaa accaacatcc atgtgaatgt ctttcatgac tgatttagag
    13561 ataattcttg aattttggaa ctagaatcta taatgagcct aaattaaaac attgtgaact
    13621 agtaagggcg aattcgaccc agctttcttg tacaaagtgg ttcgataatt cttaattaac
    13681 tagttctaga gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt tcaaacattt
    13741 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
    13801 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
    13861 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
    13921 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctgaattcgt
    13981 aatcatggtc atagaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
    14041 agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    14101 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    14161 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    14221 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga
    14281 gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
    14341 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
    14401 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
    14461 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
    14521 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
    14581 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacactct
    14641 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt
    14701 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
    14761 catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg
    14821 aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
    14881 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
    14941 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagaccttcc
    15001 tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct
    15061 acaaatctat ctctctcgag ctttcgcaga tctgtcgatc gaccatgggg attgaacaag
    15121 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    15181 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    15241 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccag gacgaggcag
    15301 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    15361 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    15421 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    15481 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    15541 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    15601 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
    15661 tcgtgacaca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    15721 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    15781 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    15841 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    15901 gagcgggact ctggggttcg gatcgatcct ctagctagag tcgatcgaca agctcgagtt
    15961 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
    16021 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
    16081 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa
    16141 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
    16201 gcgtcaattt gtttacacca caatatatcc tgcca
//
