LOCUS       EER_15_Tol2_mfap        5746 bp ds-DNA     circular     17-DEC-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    561..724
                     /locus_tag="Tol2 right arm"
                     /label="Tol2 right arm"
                     /ApEinfo_label="Tol2 right arm"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2350..3045
                     /locus_tag="BFP"
                     /label="BFP"
                     /ApEinfo_label="BFP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3136..3186
                     /locus_tag="SV40 polyA"
                     /label="SV40 polyA"
                     /ApEinfo_label="SV40 polyA"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3292..3507
                     /locus_tag="Tol2 left arm"
                     /label="Tol2 left arm"
                     /ApEinfo_label="Tol2 left arm"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4762..5622
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3561..3580
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3601..3617
                     /locus_tag="M13 Reverse (-27)"
                     /label="M13 Reverse (-27)"
                     /ApEinfo_label="M13 Reverse (-27)"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3614..3636
                     /locus_tag="M13 Reverse (-48)"
                     /label="M13 Reverse (-48)"
                     /ApEinfo_label="M13 Reverse (-48)"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(730..1069,1071..1128,1131..1134,1137..1212,1214..1280,
                     1283..1292,1294..1296,1298..1475,1477..1484,1486..1762,
                     1764..1925,1927..1942,1944..1948,1950..2031,2033..2058,
                     2060..2125,2127..2227,2229..2329)
                     /locus_tag="mfap4"
                     /label="mfap4"
                     /ApEinfo_label="mfap4"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="#ff8040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2342..2349
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#408080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
       61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
      121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
      181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT
      241 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG
      301 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCCAC AATGACGGCT ACTACATGGT
      361 GCCATTCCTT CCTCTTTATA GGAATGGAGA CTACCTCCTG TCCAACAAGG CTCTTGGATA
      421 CGAGTACGCC TACCTGTTGG ACCCAGGTCA TTGCACAACA CCAGAAATGC CCTCTGATCT
      481 GCAAAAGACG TGAATATCTG TTCAGACACC CATATCCACT CTGTTCCACA CAGGTCAGAG
      541 GTTTGTCCAG GAGTTCTTGA CAGAGGTGTA AAAAGTACTC AAAAATTTTA CTCAAGTGAA
      601 AGTACAAGTA CTTAGGGAAA ATTTTACTCA ATTAAAAGTA AAAGTATCTG GCTAGAATCT
      661 TACTTGAGTA AAAGTAAAAA AGTACTCCAT TAAAATTGTA CTTGAGTATT AAGGAAGTAA
      721 AAGGCTAGCG CGTTTCTTGG TACAGCTGGa aagggaataa cgcggtcagt atgaaaacca
      781 tcagcatcaa gatcaaacaa gtgccttaga aacagggctt ttaaaaggaa acctcttctc
      841 ttttaaatgt gtatctgaaa aatcgagtct gaaatgtatg gctgttggtg taatgacatt
      901 tttctctttg ttttcacttt aaagctttaa taaactcctg tatgcagatt tatgagattg
      961 tgtcctgcta ttctttgaaa caaaagctca ttttccattc aaataatggg aactggatta
     1021 atgtacaaaa ctgtaaaagg gcataaagca tttgtggata aagcagagtt tccatctaat
     1081 gagacaaaga caacacacaa taaatgcaac gctgtggctt ttgaaggtga gagatgctct
     1141 ttgggatgct caagacagtt ctggaggtaa taataataat aataatactg aaccactgtg
     1201 atgacagact ttaaggcatt ttacaataag caaaacaaca tttcagatgt tttacagtgt
     1261 gctggaatta aatgctgcaa agtccattga tgcgtcccat cacgcacact tttttctgat
     1321 catcatctgt tcaaataaat cacattacat atgctgcagc tttctggatt gtctgtagag
     1381 gtttatgggt gcaaggcagg agtccagcta gaagagcatt gcaatagtcc agctttttgg
     1441 actaaatgtt gtgcagtatg atctcttaag aatgggtctg atgttaaaga gtgcaaacaa
     1501 tttttcactg agggattcac acatgcagat atgtctgata atcagatcat ccatataaat
     1561 tagaaatatt aagaaatcga gtggatttac agccagattc ttagtgttcg ctcttaactt
     1621 gcgccatttg attagctctg tcacttcagg tattcttgtg tatgctgaat cagtcaatgt
     1681 taacccaaag agaacactcg actggttctt gtgttagaga atttagagtc ttgtaaatgc
     1741 agactccagg ttaacattgc acttatctct gcattgtgtc cttttactcc tcaaccaagc
     1801 ttttaaccaa ccaatgtttt atagttgttt tgaaaaaagt cattagtgtt gtgcaggttg
     1861 aatcgtgtgt ttgttatctt ctgcagaaaa gggttatttg gtggggttat cataacattt
     1921 gtctgcgttt gcagatttgg cagaaaacag aaccgagaac tgctgtcctt tcactcttcg
     1981 gttgtgaaaa cagattgttg ggtttttgag gattgcctca agcaggtttt gtcacagagc
     2041 acgagactca gcatcagcga tttaaaggag cgtttgagtt atttccagct gaacacacac
     2101 ggtgagtgac ttattctgta gagcgagatt atacgaagaa ataacctgca acaataagaa
     2161 ggtgtgagtg aagctctttt acatctcttg tagataaact gagctgttga ggagagagtg
     2221 agaagattgc agtaagttct gtggctgttt tattccttgt gacgatcaca tttacacacg
     2281 ccattttgtc tcgtattggt gattTCTTTC TTCATGACTT TAGATCGTGG TCGACggtac
     2341 CTCGCCACCA tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     2401 accgtggaca accatcactt cgagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     2461 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     2521 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
     2581 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     2641 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     2701 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     2761 ctcggctggg AGGCCTTCAC CGAGACGCTG TACCCCGCTG ACGGCGGCCT GGAAGGCAGA
     2821 AACGACATGG CCCTGAAGCT CGTGGGCGGG AGCCATCTGA TCGCAAACAT CAAGACCACA
     2881 TATAGATCCA AGAAACCCGC TAAGAACCTC AAGATGCCTG GCGTCTACTA TGTGGACTAC
     2941 AGACTGGAAA GAATCAAGGA GGCCAACAAC GAGACCTACG TCGAGCAGCA CGAGGTGGCA
     3001 GTGGCCAGAT ACTGCGACCT CCCTAGCAAA CTGGGGCACA AGTGAGTCGA TGATCTAGAA
     3061 CTATAGTGAG TCGTATTACG TAGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA
     3121 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
     3181 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT
     3241 TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA TTCGCGGCCG CAAGGGAAAA
     3301 TAGAATGAAG TGATCTCCAA AAAATAAGTA CTTTTTGACT GTAAATAAAA TTGTAAGGAG
     3361 TAAAAAGTAC TTTTTTTTCT AAAAAAATGT AATTAAGTAA AAGTAAAAGT ATTGATTTTT
     3421 AATTGTACTC AAGTAAAGTA AAAATCCCCA AAAATAATAC TTAAGTACAG TAATCAAGTA
     3481 AAATTACTCA AGTACTTTAC ACCTCTGGTT CTTGACCCCC TACCTTCAGC AAGCCCAGCA
     3541 GATCCACTAG ATCTTTTGTT CCCTTTAGTG AGGGTTAATT GCGCGCTTGG CGTAATCATG
     3601 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC
     3661 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC
     3721 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT
     3781 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
     3841 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
     3901 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
     3961 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
     4021 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     4081 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     4141 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     4201 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     4261 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     4321 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     4381 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     4441 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     4501 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     4561 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     4621 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     4681 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     4741 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     4801 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     4861 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
     4921 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     4981 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     5041 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     5101 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     5161 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     5221 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     5281 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     5341 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     5401 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     5461 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     5521 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     5581 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     5641 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     5701 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTG 
//
