LOCUS       pZP06C                  7769 bp ds-DNA     circular     01-SEP-2024
DEFINITION  .
COMMENT     remove galK from pZP06 and add sacB and mCherry=pZP06(sacBmCherry.
FEATURES             Location/Qualifiers
     misc_feature    1..58
                     /label="Tfdx"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    63..98
                     /label="galKrem-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(64..98)
                     /label="galKcm"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(99..1520)
                     /label="sacB"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1521..1753
                     /label="PrpsJfsuo"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1754..1754
                     /label="pirmeF"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1754..1754
                     /label="Pfdx"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1754..1754
                     /label="primer1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1754..2979
                     /label="galKrem-R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1783..2978
                     /label="mCherry"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(3227..4582)
                     /label="repA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4557..4579
                     /label="primer 3"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4583..4835
                     /label="Pfdx-E"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4583..4835
                     /label="Pfdx-E"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4859..4891
                     /label="primer 4"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4868..5667
                     /label="oriFn"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5959..5987
                     /label="priemr2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6154..6777)
                     /label="catP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    7035..7654
                     /label="OriEC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 TCGCGACCCA ACACTCTCCT ACATTTATTC CATCCGAAGA TGATCTTAAT AATATTAGTA
       61 TACCTAGTTT ATTTTTTTAG CACCATCAGC TATGTTTATT ATTTGTTAAC TGTTAATTGT
      121 CCTTGTTCAA GGATGCTGTC TTTGACAACA GATGTTTTCT TGCCTTTGAT GTTCAGCAGG
      181 AAGCTCGGCG CAAACGTTGA TTGTTTGTCT GCGTAGAATC CTCTGTTTGT CATATAGCTT
      241 GTAATCACGA CATTGTTTCC TTTCGCTTGA GGTACAGCGA AGTGTGAGTA AGTAAAGGTT
      301 ACATCGTTAG GATCAAGATC CATTTTTAAC ACAAGGCCAG TTTTGTTCAG CGGCTTGTAT
      361 GGGCCAGTTA AAGAATTAGA AACATAACCA AGCATGTAAA TATCGTTAGA CGTAATGCCG
      421 TCAATCGTCA TTTTTGATCC GCGGGAGTCA GTGAACAGGT ACCATTTGCC GTTCATTTTA
      481 AAGACGTTCG CGCGTTCAAT TTCATCTGTT ACTGTGTTAG ATGCAATCAG CGGTTTCATC
      541 ACTTTTTTCA GTGTGTAATC ATCGTTTAGC TCAATCATAC CGAGAGCGCC GTTTGCTAAC
      601 TCAGCCGTGC GTTTTTTATC GCTTTGCAGA AGTTTTTGAC TTTCTTGACG GAAGAATGAT
      661 GTGCTTTTGC CATAGTATGC TTTGTTAAAT AAAGATTCTT CGCCTTGGTA GCCATCTTCA
      721 GTTCCAGTGT TTGCTTCAAA TACTAAGTAT TTGTGGCCTT TATCTTCTAC GTAGTGAGGA
      781 TCTCTCAGCG TATGGTTGTC GCCTGAGCTG TAGTTGCCTT CATCGATGAA CTGCTGTACA
      841 TTTTGATACG TTTTTCCGTC ACCGTCAAAG ATTGATTTAT AATCCTCTAC ACCGTTGATG
      901 TTCAAAGAGC TGTCTGATGC TGATACGTTA ACTTGTGCAG TTGTCAGTGT TTGTTTGCCG
      961 TAATGTTTAC CGGAGAAATC AGTGTAGAAT AAACGGATTT TTCCGTCAGA TGTAAATGTG
     1021 GCTGAACCTG ACCATTCTTG TGTTTGGTCT TTTAGGATAG AATCATTTGC ATCGAATTTG
     1081 TCGCTGTCTT TAAAGACGCG GCCAGCGTTT TTCCAGCTGT CAATAGAAGT TTCGCCGACT
     1141 TTTTGATAGA ACATGTAAAT CGATGTGTCA TCCGCATTTT TAGGATCTCC GGCTAATGCA
     1201 AAGACGATGT GGTAGCCGTG ATAGTTTGCG ACAGTGCCGT CAGCGTTTTG TAATGGCCAG
     1261 CTGTCCCAAA CGTCCAGGCC TTTTGCAGAA GAGATATTTT TAATTGTGGA CGAATCAAAT
     1321 TCAGAAACTT GATATTTTTC ATTTTTTTGC TGTTCAGGGA TTTGCAGCAT ATCATGGCGT
     1381 GTAATATGGG AAATGCCGTA TGTTTCCTTA TATGGCTTTT GGTTCGTTTC TTTCGCAAAC
     1441 GCTTGAGTTG CGCCTCCTGC CAGCAGTGCG GTAGTAAAGG TTAATACTGT TGCTTGTTTT
     1501 GCAAACTTTT TGATGTTCAT TTATTTATTG CACCTCCTTT AAATTAACTA CATAAGATAA
     1561 TTCTTATGCA CTCTTGAGAG TATAACATAC TTTTTAAAAA AATAAAAGAA AAATTTTTTA
     1621 AATCATTAAA AATTTTTTTA TACTACTAAC TATTATTTTT TATTGACTTT TAATCTAAAA
     1681 AATTGTATTA TCATAGTGTA AAATTCTATA TATTTATTTA AATATTTAAA ATTTTACAAA
     1741 TTATTTTATT TTTGTTAAAA AGACTTTAGA AGAATTAAAA GCCTTAGCAA TGGTGTTGCA
     1801 ATGTCGTTTT ATACGTGTTT TAATAATCAA GTTGCTTTGA CAGGTCGCGG TTAAGCGGGC
     1861 TGGCGCAGCA ACCATGACAC CTTCATGGTG TGGGTTCAGA AATGGACCAC ACGTTAAGGA
     1921 TCCTTAAGGC CAAGGCGTTA AGCCTGTTCA ACCTTGAGGG CCGGAGTTTG AAGGGGCATG
     1981 GCAAATAAAC AGCGGCAGTA TACGTAAGGG TCACGCTTAT CGTGAGATAA AGTCCCTTCC
     2041 GGCTTGACAC TACGGGTCTT GTACCCCGTC AGGATGTTTA CGGCGTTTGT TATGTGCCAT
     2101 CGTCCTATGA ACTCCACCAA GTCGATAAGA CAGTCAAGTT AAGAACTGGC GTTGCGCCCA
     2161 GGTCCCCAAA GATCCGGACA ACGTTATAGA GAAATATTGA AGCGATTCCC ACCAATCCAA
     2221 GGATGTGGGA CAAGCGAGGA AGAGGATAAG CATATGATGG TGAGCAAGGG CGAGGAGGAT
     2281 AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC
     2341 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC
     2401 GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT
     2461 CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG
     2521 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC
     2581 GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG
     2641 CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG
     2701 GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG
     2761 AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC
     2821 AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC
     2881 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC
     2941 GGCGGCATGG ACGAGCTGTA CAAGTAGTAG GGATCCTCGA TTGAATTAGA AAAAATTGAA
     3001 AAAAGTAAAA AGAAATTAAA AGAGTTAGAA AAAAAAGTAC TAGAAGAAAG AAACAAAAAG
     3061 TTATTATATG AATTTAAAGA TTTAGATGAT TTTAATTTAG AAAAAGTAAT TAATTTTATT
     3121 CGTCAACTAA AAAAAGATAT AGAAAAGGAA GAAAAAGAAA AAGTTGAAAA AAAATGAAGC
     3181 TCTAAAATCA ATTTTAAGCC ACTTAAAAAA AGAAAGAGGT ATTATACTAT ACCTCTTTTT
     3241 TTAATTTATT TAGTTCTTCT TGCATTCTTT TTATATTTTC TTCTAATTCG TGAAGCTCTT
     3301 TTTCTTTTTC ATTAACTTCT TCTTTAGTAT TAATTATATT ATATTTTTTT GATTGACTTG
     3361 CATTAAATAT AATTTCTAAA AGTTTTGGAT CACTATTAGG AACTTCATCT TTTCGTTTTA
     3421 TTATCCAATC TTCTTTAATC TTATTATATT CTTCTTCAGT TACATCTATT TTAAATAAAA
     3481 CTTCTTGTTC TATTTGTTCA GGAGTTATAT TCTTTCTAAC TTCATAATCT GTAATGTCTT
     3541 TTTCTACTAA ATCTTTTAAT GATGTAATAG TATTTGTACT TTCTATATTT CTATTTCTTT
     3601 CTTCAACTTT TAGTTCTTCA TAGTCAGCTG GTAGATGATT TTTATATACT GCTTTTTCTT
     3661 CTAAAATTAG TTTTGAGAAA TATGCTGATT TATTTAAAAT ATTTTTATCA TTTTGTACAT
     3721 ACTCCCACTG CTCAGATAAA TATTTTTTAA TATATTCTAA ACTATTATTT TTTAATAATT
     3781 TTTCTAAAAC TTCTTTATGC TTCTTTGAAT AATTTACATC AGTAAAAGTT GATTTAAAAT
     3841 ATTTTTCTAA CTCTCCTGAA GCAATATTTT CTTTTTCTTC CACTACTTCT GCTTCTTCTA
     3901 TATTATCTTT CTTATTTTGA AAATTAAAAT CATTAGTCCA GCTAAATTTA TATCCTTTTA
     3961 TTACTCTTCC ATTTTTTAAT CGCTCAACCT TTAAATTTTC AAAAAGTGTT TTTAATTCTT
     4021 CAACAGCTGG TTTTAGAATT CTCTTATTAA AATCTGATAA ATTATTTCTA TAACTTTCTT
     4081 CAACTTCTAG AATATTAAAA AATCTCTCTT TTTCAAATTC AGCTTCTTTT TTATGACTAA
     4141 ACTCCATTAA ATAAGGAACC AATAACTTTG AATATTTTCC TGAAAGTTTT CTAACATCTG
     4201 AAAAGAGAAA CCTTAAATAA CTGTTAGCAA TATTAAAAAA ATAAAGAGAA TCTTTTGTTA
     4261 CTCTTATAGT TAAAGTATTA TTATCTAAAT CATTTATGAA TTTTTCAAAT AAAAAGATTG
     4321 TTTCAATTTT TTTATTTGTT ATATTAGTAA TATGTAAATT TCCTAATTTT TGATGAAGTC
     4381 CTAAAATATA TTTTTTTAAA TCTCTAAAAT TAATTAAACC TAAACTTTTT TTTATAGAAG
     4441 AAAAATCCAT TGTTATAATT TCATCTCTAA TATCCTTTAC ATTTAAACAT ATATAATAAA
     4501 ATAATTCTAA TTCTTTTTCA CTTAATGTCC CTATATTTAA TTTGGTAAAA TCTTTATGTA
     4561 CTTTTACTAA ATCATTATTC ATCTTGTTGT TACCTCCTTA GCAGGGTGCT GCCAAGGGCA
     4621 TCAAGACGAT GCTGGTATCA CCGGAACCTA TAGTGAGTCG TATTGGTACC GATTTATATT
     4681 TTACCATGTT TTTATTAATT TTTAAAGTAA TTTTTATATA ACTGTATAAC CTATATTTTC
     4741 TATGGATTCT ATTATCCTAT CTACATCTAC AAAATAATCA TCATATATAA CACTTACTTC
     4801 TTTTTTTTCC TTACTTATTT CACAGGCTAC TACACTTACA TCTAAAATCT TCAGCTCCTT
     4861 TTATGGAAAT TTTATTTCAT AAAAACATTA TACACCAATA ATATAAGACT GTCAATGAAA
     4921 TTTGTCCTGT TAAAGTTGAA AATTTGTCCT GTTAAAGTTG AAAATTTGTC CTGTTAAAGT
     4981 TGAAAATTTG TCCTGTTAAA GTTGAAAATT TGTCCTGTTA AAGTTGAAAA TTTGTCCTGT
     5041 TAAAGTTGAA AATTTGTCCT GTTATCTTTT TGTTTTACAT TGCTATTAAA AAGAAAAATG
     5101 AGTGCTGTAT TATATATTAT TATATAATAT TATTATATTA ATCATATTAG TAATGTGATT
     5161 TTTTTTAATA AAAGAAGCTC AATAAAAAAA TATAAAAAAT TACCGAAAGT GGAAAATCAA
     5221 CTTTCGGTAA AAAAGATTGA AAATAAATAT AAATATGTTT CCCTAAAAAA AAAGAGAGTT
     5281 TTCAAGCTCT CACACTTTAC TAACTCTCTA GCATTATTTT TTACAAGAAC TATGCTAATA
     5341 AACTTGTGAT AATGTGAAAC CATATTAATA TAACTGCATA TTGTAAAATA GCAATAAATA
     5401 TTAAAGCCAT TATCAATAGT ATCCATTCCT TGAACATCTT TCACCTCCTT TGGAGGAAAC
     5461 GACAATTAGA ATTAATAACA TATTGAAATT AAAAATTGAA TGTGATATAA TTAGTTTGAA
     5521 ACATAGCAAC AAAGTATCCG TTGCTTGAAC AAAACGACAT AAAAAGTTAG GACTCTCACA
     5581 ATGCCTAACT TTTTTTATTT AAAAAAACAT AAAGAAGTTT AACACACAAA CAGCATTTAA
     5641 AATCAATTTA AAAGTCCTAG AACTAGTGAA ATTTCCGGCT TTATTAAACT TATTTTTAGG
     5701 AATTTTATTT TCATTTTCAT CTTTACAGGA TTTGATTATA TCTTTAAATA TGTTTTATCA
     5761 AATATTATCT TTTTCTAAAT TTATATATAT TTTTATTATA TTTATTATTA TATATATTTT
     5821 ATTTTTAAGT TTCTTTCTAA CAGCTATTAA AAAGAAACTT AAAAATAAAA ACACGTACTC
     5881 TAAACCAATA AATAAAACTA TTTTTATTAT TGCTGCCTTG ATTGGAATAG TTTTTAGTAA
     5941 AATTAATTTC AATATTCCGG GCTTGGTTGT GTTGCTTTTC GGGGCTTAAA TCAAGGAAAA
     6001 ATCCTTGTTT TAAGCCTTTC AAAAAGAAAC ACAAGGTCTT TGTACTAACC TGTGGTTATG
     6061 TATAAAATTG TAGATTTTAG GGTAACAAAA AACACCGTAT TTCTACGATG TTTTTGCTTA
     6121 AATACTTGTT TTTAGTTACA GACAAACCTG AAGTTAACTA TTTATCAATT CCTGCAATTC
     6181 GTTTACAAAA CGGCAAATGT GAAATCCGTC ACATACTGCG TGATGAACTT GAATTGCCAA
     6241 AGGAAGTATA ATTTTGTTAT CTTCTTTATA ATATTTCCCC ATAGTAAAAA TAGGAATCAA
     6301 ATAATCATAT CCTTTCTGCA AATTCAGATT AAAGCCATCG AAGGTTGACC ACGGTATCAT
     6361 AGATACATTA AAAATGTTTT CCGGAGCATT TGGCTTTCCT TCCATTCTAT GATTGTTTCC
     6421 ATACCGTTGC GTATCACTTT CATAATCTGC TAAAAATGAT TTAAAGTCAG ACTTACACTC
     6481 AGTCCAAAGG CTGGAAAATG TTTCAGTATC ATTGTGAAAT ATTGTATAGC TTGGTATCAT
     6541 CTCATCATAT ATCCCCAATT CACCATCTTG ATTGATTGCC GTCCTAAACT CTGAATGGCG
     6601 GTTTACAATC ATTGCAATAT AATAAAGCAT TGCAGGATAT AGTTTCATTC CCTTTTCCTT
     6661 TATTTGTGTG ATATCCACTT TAACGGTCAT GCTGTATGTA CAAGGTACAC TTGCAAAGTA
     6721 GTGGTCAAAA TACTCTTTTC TGTTCCAACT ATTTTTATCA ATTTTTTCAA ATACCATCTA
     6781 AGTTCCCTCT CAAATTCAAG TTTATCGCTC TAATGAACAA AGATATTATA CCACATTTTT
     6841 GTGAATTTTT CAACTTGCCC ACTTCGACTG CACTCCCGAC TTAATAACTT CTTGAACACT
     6901 TGCCGAAAAA GAAAAACTGC CGAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
     6961 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
     7021 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
     7081 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
     7141 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
     7201 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
     7261 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
     7321 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
     7381 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
     7441 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
     7501 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
     7561 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
     7621 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
     7681 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT
     7741 TACCGCCTTT GAGTGAGCTG ATACCGCTC
//