LOCUS       pcDNA3.1-eIF4E.gb.xdna    6011 bp      DNA     circular   2024/12/25
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: synthetic circular DNA - [LOCUS]: Exported - [
            ACCESSION]: . - [SOURCE]: 1..6011 - [CDS]: 2719..3513
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..6011
                     /label=source
                     /SerialCloner_Color=&h2F4F4F
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=mol_type="other DNA" - organism="synthetic DNA construct" -  \ 
                     /SerialCloner_Desc=1..6011 \ 
     enhancer        235..614
                     /label=human cytomegalovirus immediate early enhancer_CMV enhancer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CMV enhancer - note="human cytomegalovirus immediate early enhancer" -  \ 
                     /SerialCloner_Desc=235..614 \ 
     promoter        615..818
                     /label=human cytomegalovirus (CMV) immediate early _CMV promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CMV promoter - note="human cytomegalovirus (CMV) immediate early  - promoter" -  \ 
                     /SerialCloner_Desc=615..818 \ 
     primer_bind     769..789
                     /label=cmv-f
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=cmv-f -  \ 
                     /SerialCloner_Desc=769..789 \ 
     promoter        863..881
                     /label=promoter for bacteriophage T7 RNA polymerase_T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 promoter - note="promoter for bacteriophage T7 RNA polymerase" -  \ 
                     /SerialCloner_Desc=863..881 \ 
     primer_bind     921..950
                     /label=Primer 1
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Primer 1 -  \ 
                     /SerialCloner_Desc=921..950 \ 
     primer_bind     complement(1550..1584)
                     /label=Primer 2
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Primer 2 -  \ 
                     /SerialCloner_Desc=complement(1550..1584) \ 
     polyA_signal    1611..1835
                     /label=bovine growth hormone polyadenylation signal_bGH poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bGH poly(A) signal - note="bovine growth hormone polyadenylation signal" -  \ 
                     /SerialCloner_Desc=1611..1835 \ 
     primer_bind     complement(1655..1672)
                     /label=bgh-r
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bgh-r -  \ 
                     /SerialCloner_Desc=complement(1655..1672) \ 
     rep_origin      1881..2309
                     /label=f1 bacteriophage origin of replication; arrow _f1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=RIGHT - label=f1 ori - note="f1 bacteriophage origin of replication; arrow  - indicates direction of (+) strand synthesis" -  \ 
                     /SerialCloner_Desc=1881..2309 \ 
     promoter        2323..2652
                     /label=SV40 enhancer and early promoter_SV40 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 promoter - note="SV40 enhancer and early promoter" -  \ 
                     /SerialCloner_Desc=2323..2652 \ 
     rep_origin      2503..2638
                     /label=SV40 origin of replication_SV40 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 ori - note="SV40 origin of replication" -  \ 
                     /SerialCloner_Desc=2503..2638 \ 
     CDS             2719..3513
                     /label=confers resistance to neomycin, kanamycin, and G418 _NeoR/KanR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="aph(3')-II (or nptII)" - product="aminoglycoside phosphotransferase from Tn5" - label=NeoR/KanR - note="confers resistance to neomycin, kanamycin, and G418  - (Geneticin(R))" - translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP - VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS - SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ - GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA - LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" -  \ 
                     /SerialCloner_Desc=2719..3513 \ 
     polyA_signal    3687..3808
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 poly(A) signal - note="SV40 polyadenylation signal" -  \ 
                     /SerialCloner_Desc=3687..3808 \ 
     primer_bind     complement(3857..3873)
                     /label=common sequencing primer, one of multiple similar _M13 rev
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 rev - note="common sequencing primer, one of multiple similar  - variants" -  \ 
                     /SerialCloner_Desc=complement(3857..3873) \ 
     protein_bind    3881..3897
                     /label=The lac repressor binds to the lac operator to _lac operator
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac operator - bound_moiety="lac repressor encoded by lacI" - note="The lac repressor binds to the lac operator to  - inhibit transcription in E. coli. This inhibition can be  - relieved by adding lactose or  - isopropyl-beta-D-thiogalactopyranoside (IPTG)." -  \ 
                     /SerialCloner_Desc=3881..3897 \ 
     promoter        complement(3905..3935)
                     /label=promoter for the E. coli lac operon_lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac promoter - note="promoter for the E. coli lac operon" -  \ 
                     /SerialCloner_Desc=complement(3905..3935) \ 
     protein_bind    3950..3971
                     /label=CAP binding activates transcription in the presence _CAP binding site
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CAP binding site - bound_moiety="E. coli catabolite activator protein" - note="CAP binding activates transcription in the presence  - of cAMP." -  \ 
                     /SerialCloner_Desc=3950..3971 \ 
     rep_origin      complement(4259..4844)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of _ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=LEFT - label=ori - note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  - replication" -  \ 
                     /SerialCloner_Desc=complement(4259..4844) \ 
     CDS             complement(5015..5875)
                     /label=confers resistance to ampicillin, carbenicillin, and_AmpR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="bla" - product="beta-lactamase" - label=AmpR - note="confers resistance to ampicillin, carbenicillin, and - related antibiotics" - translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS - PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW - EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA - LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS - LIKHW" -  \ 
                     /SerialCloner_Desc=complement(5015..5875) \ 
     promoter        complement(5876..5980)
                     /label=AmpR promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=gene="bla" - label=AmpR promoter -  \ 
                     /SerialCloner_Desc=complement(5876..5980) \ 
     misc_feature    927..1580
                     /label=eIF4E (Human) ORF
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=ENST00000450253.7  NM_001968.5 \ 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaacgg gccctctaga ctcgagatgg cgactgtcga accggaaacc acccctactc 
      961 ctaatccccc gactacagaa gaggagaaaa cggaatctaa tcaggaggtt gctaacccag 
     1021 aacactatat taaacatccc ctacagaaca gatgggcact ctggtttttt aaaaatgata 
     1081 aaagcaaaac ttggcaagca aacctgcggc tgatctccaa gtttgatact gttgaagact 
     1141 tttgggctct gtacaaccat atccagttgt ctagtaattt aatgcctggc tgtgactact 
     1201 cactttttaa ggatggtatt gagcctatgt gggaagatga gaaaaacaaa cggggaggac 
     1261 gatggctaat tacattgaac aaacagcaga gacgaagtga cctcgatcgc ttttggctag 
     1321 agacacttct gtgccttatt ggagaatctt ttgatgacta cagtgatgat gtatgtggcg 
     1381 ctgttgttaa tgttagagct aaaggtgata agatagcaat atggactact gaatgtgaaa 
     1441 acagagaagc tgttacacat atagggaggg tatacaagga aaggttagga cttcctccaa 
     1501 agatagtgat tggttatcag tcccacgcag acacagctac taagagcggc tccaccacta 
     1561 aaaataggtt tgttgtttaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc 
     1621 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     1681 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     1741 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     1801 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
     1861 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     1921 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     1981 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     2041 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     2101 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     2161 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     2221 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     2281 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 
     2341 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 
     2401 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     2461 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 
     2521 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 
     2581 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 
     2641 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 
     2701 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 
     2761 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 
     2821 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 
     2881 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 
     2941 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 
     3001 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 
     3061 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 
     3121 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 
     3181 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 
     3241 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 
     3301 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 
     3361 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 
     3421 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 
     3481 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 
     3541 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 
     3601 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 
     3661 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 
     3721 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     3781 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 
     3841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     3901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     3961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     4021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     4081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     4141 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     4201 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     4261 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     4321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     4381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     4441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     4501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     4561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     4621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     4681 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     4741 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt 
     4801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     4861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     4921 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     4981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     5041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     5101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     5161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     5221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     5281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     5341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     5401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     5461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     5521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     5581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     5641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     5701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     5761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     5821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     5881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     5941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     6001 cacctgacgt c
//