LOCUS       pcDNA3.1-IARS2-K664A_K667A.gb.xdna    8445 bp      DNA     circular   2024/12/25
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: synthetic circular DNA - [LOCUS]: Exported - [
            ACCESSION]: . - [SOURCE]: 1..8445 - [CDS]: 5153..5947
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..8445
                     /label=source
                     /SerialCloner_Color=&h2F4F4F
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=mol_type="other DNA" - organism="synthetic DNA construct" -  \ 
                     /SerialCloner_Desc=1..8445 \ 
     enhancer        235..614
                     /label=human cytomegalovirus immediate early enhancer_CMV enhancer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CMV enhancer - note="human cytomegalovirus immediate early enhancer" -  \ 
                     /SerialCloner_Desc=235..614 \ 
     promoter        615..818
                     /label=human cytomegalovirus (CMV) immediate early _CMV promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CMV promoter - note="human cytomegalovirus (CMV) immediate early  - promoter" -  \ 
                     /SerialCloner_Desc=615..818 \ 
     primer_bind     769..789
                     /label=cmv-f
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=cmv-f -  \ 
                     /SerialCloner_Desc=769..789 \ 
     promoter        863..881
                     /label=promoter for bacteriophage T7 RNA polymerase_T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 promoter - note="promoter for bacteriophage T7 RNA polymerase" -  \ 
                     /SerialCloner_Desc=863..881 \ 
     primer_bind     1077..1098
                     /label=primer_bind
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= \ 
                     /SerialCloner_Desc=1077..1098 \ 
                     ..
                     /label=
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= \ 
                     /SerialCloner_Desc= \ 
     primer_bind     complement(1252..1287)
                     /label=iars2-tb-H112A+P115A-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-H112A+P115A-R -  \ 
                     /SerialCloner_Desc=complement(1252..1287) \ 
     primer_bind     1252..1287
                     /label=iars2-tb-H112A+P115A-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-H112A+P115A-F -  \ 
                     /SerialCloner_Desc=1252..1287 \ 
     RBS             1274..1282
                     /label=full consensus sequence for ribosome-binding sites _Shine-Dalgarno sequence
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Shine-Dalgarno sequence - note="full consensus sequence for ribosome-binding sites  - upstream of start codons in E. coli; complementary to a  - region in the 3' end of the 16S rRNA (Chen et al., 1994)" -  \ 
                     /SerialCloner_Desc=1274..1282 \ 
     primer_bind     1580..1602
                     /label=pc3.1-IARS2-P1
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=pc3.1-IARS2-P1 -  \ 
                     /SerialCloner_Desc=1580..1602 \ 
     primer_bind     complement(2015..2048)
                     /label=9突变-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=9突变-R -  \ 
                     /SerialCloner_Desc=complement(2015..2048) \ 
     primer_bind     2015..2048
                     /label=exon9 t突变F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=exon9 t突变F -  \ 
                     /SerialCloner_Desc=2015..2048 \ 
     primer_bind     2078..2097
                     /label=pc3.1-IARS2-P2
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=pc3.1-IARS2-P2 -  \ 
                     /SerialCloner_Desc=2078..2097 \ 
     RBS             2295..2306
                     /label=strong bacterial ribosome binding site (Elowitz and_RBS
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="strong bacterial ribosome binding site (Elowitz and - Leibler, 2000)" -  \ 
                     /SerialCloner_Desc=2295..2306 \ 
     primer_bind     2567..2586
                     /label=pc3.1-IARS2-P3
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=pc3.1-IARS2-P3 -  \ 
                     /SerialCloner_Desc=2567..2586 \ 
     primer_bind     complement(2908..2947)
                     /label=iars2-tb-K664A+K667A-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-K664A+K667A-R -  \ 
                     /SerialCloner_Desc=complement(2908..2947) \ 
     primer_bind     2908..2947
                     /label=iars2-tb-K664A+K667A-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-K664A+K667A-F -  \ 
                     /SerialCloner_Desc=2908..2947 \ 
     primer_bind     3076..3094
                     /label=pc3.1-IARS2-P4
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=pc3.1-IARS2-P4 -  \ 
                     /SerialCloner_Desc=3076..3094 \ 
     primer_bind     3559..3578
                     /label=Primer 5（先不订）
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Primer 5（先不订） -  \ 
                     /SerialCloner_Desc=3559..3578 \ 
     polyA_signal    4045..4269
                     /label=bovine growth hormone polyadenylation signal_bGH poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bGH poly(A) signal - note="bovine growth hormone polyadenylation signal" -  \ 
                     /SerialCloner_Desc=4045..4269 \ 
     primer_bind     complement(4089..4106)
                     /label=bgh-r
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bgh-r -  \ 
                     /SerialCloner_Desc=complement(4089..4106) \ 
     rep_origin      4315..4743
                     /label=f1 bacteriophage origin of replication; arrow _f1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=RIGHT - label=f1 ori - note="f1 bacteriophage origin of replication; arrow  - indicates direction of (+) strand synthesis" -  \ 
                     /SerialCloner_Desc=4315..4743 \ 
     promoter        4757..5086
                     /label=SV40 enhancer and early promoter_SV40 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 promoter - note="SV40 enhancer and early promoter" -  \ 
                     /SerialCloner_Desc=4757..5086 \ 
     rep_origin      4937..5072
                     /label=SV40 origin of replication_SV40 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 ori - note="SV40 origin of replication" -  \ 
                     /SerialCloner_Desc=4937..5072 \ 
     CDS             5153..5947
                     /label=confers resistance to neomycin, kanamycin, and G418 _NeoR/KanR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="aph(3')-II (or nptII)" - product="aminoglycoside phosphotransferase from Tn5" - label=NeoR/KanR - note="confers resistance to neomycin, kanamycin, and G418  - (Geneticin(R))" - translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP - VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS - SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ - GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA - LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" -  \ 
                     /SerialCloner_Desc=5153..5947 \ 
     polyA_signal    6121..6242
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 poly(A) signal - note="SV40 polyadenylation signal" -  \ 
                     /SerialCloner_Desc=6121..6242 \ 
     primer_bind     complement(6291..6307)
                     /label=common sequencing primer, one of multiple similar _M13 rev
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 rev - note="common sequencing primer, one of multiple similar  - variants" -  \ 
                     /SerialCloner_Desc=complement(6291..6307) \ 
     protein_bind    6315..6331
                     /label=The lac repressor binds to the lac operator to _lac operator
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac operator - bound_moiety="lac repressor encoded by lacI" - note="The lac repressor binds to the lac operator to  - inhibit transcription in E. coli. This inhibition can be  - relieved by adding lactose or  - isopropyl-beta-D-thiogalactopyranoside (IPTG)." -  \ 
                     /SerialCloner_Desc=6315..6331 \ 
     promoter        complement(6339..6369)
                     /label=promoter for the E. coli lac operon_lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac promoter - note="promoter for the E. coli lac operon" -  \ 
                     /SerialCloner_Desc=complement(6339..6369) \ 
     protein_bind    6384..6405
                     /label=CAP binding activates transcription in the presence _CAP binding site
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CAP binding site - bound_moiety="E. coli catabolite activator protein" - note="CAP binding activates transcription in the presence  - of cAMP." -  \ 
                     /SerialCloner_Desc=6384..6405 \ 
     rep_origin      complement(6693..7278)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of _ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=LEFT - label=ori - note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  - replication" -  \ 
                     /SerialCloner_Desc=complement(6693..7278) \ 
     CDS             complement(7449..8309)
                     /label=confers resistance to ampicillin, carbenicillin, and_AmpR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="bla" - product="beta-lactamase" - label=AmpR - note="confers resistance to ampicillin, carbenicillin, and - related antibiotics" - translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS - PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW - EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA - LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS - LIKHW" -  \ 
                     /SerialCloner_Desc=complement(7449..8309) \ 
     promoter        complement(8310..8414)
                     /label=AmpR promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=gene="bla" - label=AmpR promoter -  \ 
                     /SerialCloner_Desc=complement(8310..8414) \ 
     misc_feature    933..3968
                     /label=IARS2 (Human) ORF
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3969..3971
                     /label=Stop Codon
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2922..2924
                     /label=K664A
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2931..2933
                     /label=K667A
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaacgg gccctctaga ctcgaccgga ccatgcgttg ggggctgcgc cctcgcgggc 
      961 cgggcgcggc cgccctggcc actgcccgaa gtttgtgggg gacgccccgc cttccctgca 
     1021 gcccgggatg gcaaggggcg acgaagaggc ttctggtgcg gtcggtctcc ggggccagta 
     1081 accaccagcc gaactcgaat agtggcagat accgggacac ggtgctgctg ccgcagacga 
     1141 gcttccccat gaagctgctg ggccgccagc agccggacac ggagctggag atccagcaga 
     1201 aatgtggatt ttcagaactt tattcatggc aaagagaaag aaaagtaaag acagaatttt 
     1261 gccttcatga tggacctcct tatgcaaacg gtgaccctca tgttggacat gctttaaata 
     1321 agattttgaa agacatagcc aatcgattcc atatgatgaa tggctccaaa atacattttg 
     1381 tgcccggctg ggattgtcat gggttgccca ttgaaataaa agtattatca gaacttggta 
     1441 gagaagctca gaatctttca gctatggaaa ttagaaagaa agctagatca tttgctaaag 
     1501 cagccattga gaaacagaaa tcagcattta ttcgttgggg aataatggca gattggaata 
     1561 attgctacta tacatttgat gggaagtatg aagccaaaca gttgagaact ttttaccaaa 
     1621 tgtatgataa gggcttggtt tatcgatctt acaaacctgt gttttggtct ccgtcatcta 
     1681 ggactgcatt ggctgaagca gaacttgaat ataatcctga gcatgtcagt cgttcaatat 
     1741 atgtaaaatt tcctctctta aagccttctc caaaattggc atctcttata gatggttcat 
     1801 ctcctgttag tattttggtc tggaccacac aaccttggac gattccagcc aatgaagctg 
     1861 tttgctatat gcctgaatca aagtatgctg ttgtgaaatg ttctaagtct ggagacctct 
     1921 acgtactggc ggcagataaa gtagcatctg ttgcttctac tttggaaaca acatttgaga 
     1981 ctatttcaac actttcaggt gtagatttgg aaaatggtac ttgcagccac cctctgattc 
     2041 ctgataaagc ctctcctctt ttacctgcaa atcatgtgac catggcaaaa ggaacgggat 
     2101 tggttcacac agccccagct catggtatgg aagactacgg tgtagcgtct cagcacaacc 
     2161 tgcccatgga ttgtctagtg gacgaagatg gagttttcac agatgttgca ggtcctgaac 
     2221 ttcaaaacaa ggctgtcctt gaagagggaa ctgatgtggt tataaagatg cttcagactg 
     2281 caaagaattt gttgaaagag gagaaattgg tgcatagcta tccgtatgac tggaggacca 
     2341 agaaacctgt ggttattcgt gccagcaagc agtggtttat aaacatcacg gatattaaga 
     2401 ctgcagccaa ggaattgtta aaaaaggtga aatttattcc tggatcagca ctgaatggca 
     2461 tggttgaaat gatggacagg cggccatatt ggtgtatatc aaggcaaaga gtttggggtg 
     2521 ttccaattcc tgtgtttcat cataagacca aggatgaata cttgatcaac agccaaacca 
     2581 ctgagcatat tgttaaacta gtggaacaac acggcagtga tatctggtgg actcttcccc 
     2641 ctgaacaact tcttccaaaa gaagtcttat ctgaggttgg tggccctgat gccttggaat 
     2701 atgtgccagg tcaggatatt ttggacatct ggtttgatag cggaacttca tggtcttatg 
     2761 ttcttccagg tcctgaccaa agagcagatt tgtacttgga aggaaaagac cagctcgggg 
     2821 gttggtttca gtcatcctta ttaacaagtg tggcagcaag gaagagagca ccttataaga 
     2881 cagtgattgt tcatggattt acccttggag aaaagggaga agccatgtcc gcctctcttg 
     2941 ggaatgtcat tcatcctgat gttgtcgtta atggaggaca agatcaaagc aaagagcctc 
     3001 cgtatggtgc tgatgtcctt cgctggtggg tagctgattc caatgtcttc accgaagttg 
     3061 caattggccc atccgtgctc aatgctgcca gagatgatat tagcaagctt aggaatacac 
     3121 ttcgctttct tttgggaaat gtggctgatt tcaacccaga aacagattcc atccctgtaa 
     3181 acgatatgta tgtcatagac cagtacatgc tacacttact gcaggatttg gcaaacaaga 
     3241 ttaccgaatt atacaaacaa tatgattttg gaaaagttgt tcggctgtta cggacgtttt 
     3301 ataccagaga gctctctaac ttttatttca gtataatcaa agataggctc tattgtgaaa 
     3361 aggaaaatga ccccaaacga cgctcttgtc agactgcatt agttgaaatt ttggatgtaa 
     3421 tagttcgttc ttttgctccc attcttcctc acctggctga agaggtgttc cagcacatac 
     3481 cttatattaa agagcccaag agtgttttcc gtactgggtg gattagtact agttctatct 
     3541 ggaaaaagcc cgggttggaa gaagctgtgg agagtgcgtg tgcaatgcga gactcatttc 
     3601 ttggaagcat ccctggcaaa aatgcagctg agtacaaggt tatcactgtg atagaacctg 
     3661 gactgctttt tgagataata gagatgctgc agtctgaaga gacttccagc acctctcagt 
     3721 tgaatgaatt aatgatggct tctgagtcaa ctttactggc tcaggaacca cgagagatga 
     3781 ctgcagatgt aatcgagctt aaagggaaat tcctcatcaa cttagaaggt ggtgatattc 
     3841 gtgaagagtc ttcctataaa gtaattgtca tgccgactac gaaagaaaaa tgcccccgtt 
     3901 gttggaagta tacagcggag tcttcagata cactgtgtcc tcgatgtgca gaagttgtca 
     3961 gtggaaaata ggaattccac cacactggac tagtggatcc gagctcggta ccaagcttaa 
     4021 gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 
     4081 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 
     4141 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 
     4201 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 
     4261 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 
     4321 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     4381 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     4441 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     4501 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
     4561 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     4621 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
     4681 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
     4741 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 
     4801 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
     4861 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     4921 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     4981 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 
     5041 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 
     5101 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 
     5161 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 
     5221 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 
     5281 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 
     5341 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 
     5401 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 
     5461 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 
     5521 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 
     5581 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 
     5641 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 
     5701 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 
     5761 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 
     5821 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 
     5881 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 
     5941 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 
     6001 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 
     6061 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 
     6121 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     6181 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     6241 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 
     6301 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     6361 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
     6421 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 
     6481 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     6541 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     6601 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     6661 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     6721 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     6781 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     6841 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     6901 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     6961 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     7021 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     7081 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 
     7141 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     7201 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc 
     7261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     7321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     7381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     7441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     7501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     7561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     7621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     7681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     7741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     7801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     7861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     7921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     7981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     8041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     8101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     8161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     8221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     8281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     8341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     8401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//