LOCUS       pcDNA3.1-flag-IARS2-?1-48.gb.xdna    8350 bp      DNA     circular   2024/12/25
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: synthetic circular DNA - [LOCUS]: Exported - [
            ACCESSION]: . - [SOURCE]: 1..8350 - [CDS]: 934..957
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..8350
                     /label=source
                     /SerialCloner_Color=&h2F4F4F
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=mol_type="other DNA" - organism="synthetic DNA construct" -  \ 
                     /SerialCloner_Desc=1..8350 \ 
     enhancer        235..614
                     /label=human cytomegalovirus immediate early enhancer_CMV enhancer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CMV enhancer - note="human cytomegalovirus immediate early enhancer" -  \ 
                     /SerialCloner_Desc=235..614 \ 
     promoter        615..818
                     /label=human cytomegalovirus (CMV) immediate early _CMV promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CMV promoter - note="human cytomegalovirus (CMV) immediate early  - promoter" -  \ 
                     /SerialCloner_Desc=615..818 \ 
     primer_bind     769..789
                     /label=cmv-f
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=cmv-f -  \ 
                     /SerialCloner_Desc=769..789 \ 
     promoter        863..881
                     /label=promoter for bacteriophage T7 RNA polymerase_T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 promoter - note="promoter for bacteriophage T7 RNA polymerase" -  \ 
                     /SerialCloner_Desc=863..881 \ 
     CDS             934..957
                     /label=FLAG
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - product="FLAG(R) epitope tag, followed by an enterokinase  - cleavage site" - label=FLAG - translation="DYKDDDDK" -  \ 
                     /SerialCloner_Desc=934..957 \ 
     primer_bind     968..1003
                     /label=IARS2-SAIKZATG-delta-1-48-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=IARS2-SAIKZATG-delta-1-48-F -  \ 
                     /SerialCloner_Desc=968..1003 \ 
     primer_bind     1157..1192
                     /label=iars2-tb-H112A+P115A-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-H112A+P115A-F -  \ 
                     /SerialCloner_Desc=1157..1192 \ 
     primer_bind     complement(1157..1192)
                     /label=iars2-tb-H112A+P115A-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-H112A+P115A-R -  \ 
                     /SerialCloner_Desc=complement(1157..1192) \ 
     RBS             1179..1187
                     /label=full consensus sequence for ribosome-binding sites _Shine-Dalgarno sequence
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Shine-Dalgarno sequence - note="full consensus sequence for ribosome-binding sites  - upstream of start codons in E. coli; complementary to a  - region in the 3' end of the 16S rRNA (Chen et al., 1994)" -  \ 
                     /SerialCloner_Desc=1179..1187 \ 
     primer_bind     1920..1953
                     /label=9突变F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=9突变F -  \ 
                     /SerialCloner_Desc=1920..1953 \ 
     primer_bind     complement(1920..1953)
                     /label=9突变-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=9突变-R -  \ 
                     /SerialCloner_Desc=complement(1920..1953) \ 
     RBS             2200..2211
                     /label=strong bacterial ribosome binding site (Elowitz and_RBS
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="strong bacterial ribosome binding site (Elowitz and - Leibler, 2000)" -  \ 
                     /SerialCloner_Desc=2200..2211 \ 
     primer_bind     2813..2852
                     /label=iars2-tb-K664A+K667A-F
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-K664A+K667A-F -  \ 
                     /SerialCloner_Desc=2813..2852 \ 
     primer_bind     complement(2813..2852)
                     /label=iars2-tb-K664A+K667A-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=iars2-tb-K664A+K667A-R -  \ 
                     /SerialCloner_Desc=complement(2813..2852) \ 
     primer_bind     complement(3848..3882)
                     /label=IARS2-ECORITAG-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=IARS2-ECORITAG-R -  \ 
                     /SerialCloner_Desc=complement(3848..3882) \ 
     polyA_signal    3950..4174
                     /label=bovine growth hormone polyadenylation signal_bGH poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bGH poly(A) signal - note="bovine growth hormone polyadenylation signal" -  \ 
                     /SerialCloner_Desc=3950..4174 \ 
     primer_bind     complement(3994..4011)
                     /label=bgh-r
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bgh-r -  \ 
                     /SerialCloner_Desc=complement(3994..4011) \ 
     rep_origin      4220..4648
                     /label=f1 bacteriophage origin of replication; arrow _f1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=RIGHT - label=f1 ori - note="f1 bacteriophage origin of replication; arrow  - indicates direction of (+) strand synthesis" -  \ 
                     /SerialCloner_Desc=4220..4648 \ 
     promoter        4662..4991
                     /label=SV40 enhancer and early promoter_SV40 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 promoter - note="SV40 enhancer and early promoter" -  \ 
                     /SerialCloner_Desc=4662..4991 \ 
     rep_origin      4842..4977
                     /label=SV40 origin of replication_SV40 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 ori - note="SV40 origin of replication" -  \ 
                     /SerialCloner_Desc=4842..4977 \ 
     CDS             5058..5852
                     /label=confers resistance to neomycin, kanamycin, and G418 _NeoR/KanR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="aph(3')-II (or nptII)" - product="aminoglycoside phosphotransferase from Tn5" - label=NeoR/KanR - note="confers resistance to neomycin, kanamycin, and G418  - (Geneticin(R))" - translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP - VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS - SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ - GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA - LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" -  \ 
                     /SerialCloner_Desc=5058..5852 \ 
     polyA_signal    6026..6147
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 poly(A) signal - note="SV40 polyadenylation signal" -  \ 
                     /SerialCloner_Desc=6026..6147 \ 
     primer_bind     complement(6196..6212)
                     /label=common sequencing primer, one of multiple similar _M13 rev
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 rev - note="common sequencing primer, one of multiple similar  - variants" -  \ 
                     /SerialCloner_Desc=complement(6196..6212) \ 
     protein_bind    6220..6236
                     /label=The lac repressor binds to the lac operator to _lac operator
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac operator - bound_moiety="lac repressor encoded by lacI" - note="The lac repressor binds to the lac operator to  - inhibit transcription in E. coli. This inhibition can be  - relieved by adding lactose or  - isopropyl-beta-D-thiogalactopyranoside (IPTG)." -  \ 
                     /SerialCloner_Desc=6220..6236 \ 
     promoter        complement(6244..6274)
                     /label=promoter for the E. coli lac operon_lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac promoter - note="promoter for the E. coli lac operon" -  \ 
                     /SerialCloner_Desc=complement(6244..6274) \ 
     protein_bind    6289..6310
                     /label=CAP binding activates transcription in the presence _CAP binding site
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CAP binding site - bound_moiety="E. coli catabolite activator protein" - note="CAP binding activates transcription in the presence  - of cAMP." -  \ 
                     /SerialCloner_Desc=6289..6310 \ 
     rep_origin      complement(6598..7183)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of _ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=LEFT - label=ori - note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  - replication" -  \ 
                     /SerialCloner_Desc=complement(6598..7183) \ 
     CDS             complement(7354..8214)
                     /label=confers resistance to ampicillin, carbenicillin, and_AmpR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="bla" - product="beta-lactamase" - label=AmpR - note="confers resistance to ampicillin, carbenicillin, and - related antibiotics" - translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS - PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW - EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA - LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS - LIKHW" -  \ 
                     /SerialCloner_Desc=complement(7354..8214) \ 
     promoter        complement(8215..8319)
                     /label=AmpR promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=gene="bla" - label=AmpR promoter -  \ 
                     /SerialCloner_Desc=complement(8215..8319) \ 
     misc_feature    931..3873
                     /label=IARS2 (human) ?1~48  N-Flag
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    934..957
                     /label=Flag tag
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaacgg gccctctaga ctcgaccacc atggactaca aggacgacga tgataagggc 
      961 ggatccctcg accggaccat gagtaaccac cagccgaact cgaatagtgg cagataccgg 
     1021 gacacggtgc tgctgccgca gacgagcttc cccatgaagc tgctgggccg ccagcagccg 
     1081 gacacggagc tggagatcca gcagaaatgt ggattttcag aactttattc atggcaaaga 
     1141 gaaagaaaag taaagacaga attttgcctt catgatggac ctccttatgc aaacggtgac 
     1201 cctcatgttg gacatgcttt aaataagatt ttgaaagaca tagccaatcg attccatatg 
     1261 atgaatggct ccaaaataca ttttgtgccc ggctgggatt gtcatgggtt gcccattgaa 
     1321 ataaaagtat tatcagaact tggtagagaa gctcagaatc tttcagctat ggaaattaga 
     1381 aagaaagcta gatcatttgc taaagcagcc attgagaaac agaaatcagc atttattcgt 
     1441 tggggaataa tggcagattg gaataattgc tactatacat ttgatgggaa gtatgaagcc 
     1501 aaacagttga gaacttttta ccaaatgtat gataagggct tggtttatcg atcttacaaa 
     1561 cctgtgtttt ggtctccgtc atctaggact gcattggctg aagcagaact tgaatataat 
     1621 cctgagcatg tcagtcgttc aatatatgta aaatttcctc tcttaaagcc ttctccaaaa 
     1681 ttggcatctc ttatagatgg ttcatctcct gttagtattt tggtctggac cacacaacct 
     1741 tggacgattc cagccaatga agctgtttgc tatatgcctg aatcaaagta tgctgttgtg 
     1801 aaatgttcta agtctggaga cctctacgta ctggcggcag ataaagtagc atctgttgct 
     1861 tctactttgg aaacaacatt tgagactatt tcaacacttt caggtgtaga tttggaaaat 
     1921 ggtacttgca gccaccctct gattcctgat aaagcctctc ctcttttacc tgcaaatcat 
     1981 gtgaccatgg caaaaggaac gggattggtt cacacagccc cagctcatgg tatggaagac 
     2041 tacggtgtag cgtctcagca caacctgccc atggattgtc tagtggacga agatggagtt 
     2101 ttcacagatg ttgcaggtcc tgaacttcaa aacaaggctg tccttgaaga gggaactgat 
     2161 gtggttataa agatgcttca gactgcaaag aatttgttga aagaggagaa attggtgcat 
     2221 agctatccgt atgactggag gaccaagaaa cctgtggtta ttcgtgccag caagcagtgg 
     2281 tttataaaca tcacggatat taagactgca gccaaggaat tgttaaaaaa ggtgaaattt 
     2341 attcctggat cagcactgaa tggcatggtt gaaatgatgg acaggcggcc atattggtgt 
     2401 atatcaaggc aaagagtttg gggtgttcca attcctgtgt ttcatcataa gaccaaggat 
     2461 gaatacttga tcaacagcca aaccactgag catattgtta aactagtgga acaacacggc 
     2521 agtgatatct ggtggactct tccccctgaa caacttcttc caaaagaagt cttatctgag 
     2581 gttggtggcc ctgatgcctt ggaatatgtg ccaggtcagg atattttgga catctggttt 
     2641 gatagcggaa cttcatggtc ttatgttctt ccaggtcctg accaaagagc agatttgtac 
     2701 ttggaaggaa aagaccagct cgggggttgg tttcagtcat ccttattaac aagtgtggca 
     2761 gcaaggaaga gagcacctta taagacagtg attgttcatg gatttaccct tggagaaaag 
     2821 ggagaaaaga tgtccaagtc tcttgggaat gtcattcatc ctgatgttgt cgttaatgga 
     2881 ggacaagatc aaagcaaaga gcctccgtat ggtgctgatg tccttcgctg gtgggtagct 
     2941 gattccaatg tcttcaccga agttgcaatt ggcccatccg tgctcaatgc tgccagagat 
     3001 gatattagca agcttaggaa tacacttcgc tttcttttgg gaaatgtggc tgatttcaac 
     3061 ccagaaacag attccatccc tgtaaacgat atgtatgtca tagaccagta catgctacac 
     3121 ttactgcagg atttggcaaa caagattacc gaattataca aacaatatga ttttggaaaa 
     3181 gttgttcggc tgttacggac gttttatacc agagagctct ctaactttta tttcagtata 
     3241 atcaaagata ggctctattg tgaaaaggaa aatgacccca aacgacgctc ttgtcagact 
     3301 gcattagttg aaattttgga tgtaatagtt cgttcttttg ctcccattct tcctcacctg 
     3361 gctgaagagg tgttccagca cataccttat attaaagagc ccaagagtgt tttccgtact 
     3421 gggtggatta gtactagttc tatctggaaa aagcccgggt tggaagaagc tgtggagagt 
     3481 gcgtgtgcaa tgcgagactc atttcttgga agcatccctg gcaaaaatgc agctgagtac 
     3541 aaggttatca ctgtgataga acctggactg ctttttgaga taatagagat gctgcagtct 
     3601 gaagagactt ccagcacctc tcagttgaat gaattaatga tggcttctga gtcaacttta 
     3661 ctggctcagg aaccacgaga gatgactgca gatgtaatcg agcttaaagg gaaattcctc 
     3721 atcaacttag aaggtggtga tattcgtgaa gagtcttcct ataaagtaat tgtcatgccg 
     3781 actacgaaag aaaaatgccc ccgttgttgg aagtatacag cggagtcttc agatacactg 
     3841 tgtcctcgat gtgcagaagt tgtcagtgga aaataggaat tccaccacac tggactagtg 
     3901 gatccgagct cggtaccaag cttaagttta aaccgctgat cagcctcgac tgtgccttct 
     3961 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 
     4021 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 
     4081 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 
     4141 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 
     4201 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 
     4261 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 
     4321 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 
     4381 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 
     4441 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 
     4501 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 
     4561 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 
     4621 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 
     4681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
     4741 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
     4801 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 
     4861 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     4921 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     4981 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag 
     5041 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 
     5101 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 
     5161 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 
     5221 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 
     5281 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 
     5341 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 
     5401 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 
     5461 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 
     5521 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 
     5581 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 
     5641 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 
     5701 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 
     5761 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 
     5821 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 
     5881 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 
     5941 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 
     6001 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata 
     6061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     6121 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag 
     6181 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     6241 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 
     6301 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     6361 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     6421 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     6481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     6541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     6601 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     6661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     6721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     6781 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     6841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     6901 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     6961 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     7021 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     7081 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt 
     7141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     7201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     7261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     7321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     7381 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     7441 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     7501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     7561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     7621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     7681 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     7741 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     7801 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     7861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     7921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     7981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     8041 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     8101 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     8161 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     8221 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     8281 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     8341 acctgacgtc 
//