LOCUS       Exported                7143 bp ds-DNA     circular SYN 05-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-DIO-ATLASsnFlp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Don Arnold
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 5, 2025 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1139..2434
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /note="AAV2 ITR"
     promoter        175..622
                     /note="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    623..1285
                     /note="seq4"
     protein_bind    653..686
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    737..770
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(779..3496)
                     /codon_start=1
                     /note="ATLASsnFlp"
                     /translation="MEVQLEESGGGLVRPGGSLRLSCAASGFPFSKYFMSWVRQAPGKG
                     LEWVSTISASGNYETYTESVKGRFTIARDNAKNTLYLQMNSLKPEDTAVYYCAKGSWAR
                     DMTRGQGTQVTVSSEPKGGGSQLMMIILGVICAIILIIIIVYFSTTSPSRLEEELRRRL
                     TEPELASEISEVNLDAEFRASMLEVKEASPTSIQISWSRRDQVIRYYRITYGETGGNSP
                     VQKFTVPGSKSTATISGLKPGVDYTITVYAVTPFANFWDWHGPISINYRTGSDYKDDDD
                     KGSGSSRGSGSGYVSRHPGGGCGYVSRHPGGGCGYVSRHPGGGCGYVSRHPGGGCKLGS
                     EQKLISEEDLGSCTMAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRIGAPGSFQCRICMRNFSDRSNLSRHIR
                     THTGEKPFACDICGRKFAISSNLNSHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTH
                     TGEKPFACDICGRKFATSGNLTRHTKIHLRGSQL"
     misc_feature    1711..2597
                     /note="seq"
     misc_feature    2598..2897
                     /note="Seq 3"
     misc_feature    2898..3621
                     /note="seq2"
     protein_bind    complement(3503..3536)
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    complement(3587..3620)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3645..4233
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4116..4127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     misc_feature    4263..4383
                     /note="SV40p"
     repeat_region   4406..4546
                     /note="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4621..5076
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5358..5462
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             5463..6323
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6494..7082
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agggtaccgg atcctctaga gtcgactccg gaataacttc
      661 gtataggata ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc
      721 aatatgaaac ctcttaataa cttcgtatag catacattat acgaagttat ggcgcgccct
      781 atagctggct gccccgcagg tgaatctttg tgtgccgggt cagattgccg gaggtggcaa
      841 actttctgcc acagatatca caggcaaaag gtttttcacc agtatgtgtc cggatgtgtc
      901 tggccaggtt gtcgcttctg ctaaagtttc tcatacaaat tctgcactgg aaaggtttct
      961 ggctgccagt gtggatcttg gtgtggctgt tcaggttgct gctgatggcg aacttccggc
     1021 cgcaaatgtc gcaggcgaag ggcttttctc cggtgtgggt tctgatgtgc ctgctcaggt
     1081 tggaccggtc gctgaagttc cgcatgcaga tccggcactg gaagctaccg ggcgcgccga
     1141 tccgcctgtt gatgtagctg ctcaggtagt ccagcacctc ctggctgatg atgccgttcc
     1201 aggcggggta tctgatgctg ccctcggcgc tgcccttcag ctgctcgatg tgctgccact
     1261 cctcgatggg gttggtctcg tccttcaggg cgatcatctc cttgctgatg gggtcgtagg
     1321 cgtagtacct ggacaccagg gcgaagtagt ggtcggggat ggcggtgatc tggtgggtgt
     1381 aggtggtcct ggccacggcg gaggccctct tgtcgctcca gttgcccacc acgtttgtca
     1441 gctcggtcag gcccttcatg ctcagaaagc tggtcatcag gtgcctgccg atgtggctct
     1501 tagggccgtt cttgatagcg aagatggggt agggggcgtt cttcttcagg gccttgttgt
     1561 agctgcgcac caggttgtcc ttcagcagct ggtactcctg cttgttgctg ctgctgttgc
     1621 cggtcctgtt cactctcttc agcacgggct cgctgttcct caggaactcg tccaggtaca
     1681 ccagggggtc gatcctgcct ctggcgctga aaaagtagat gtgcctggac acgcttgtct
     1741 tggtctcggt caccaggcac tgaatgatca cgcccaggta cttgttctgc accagcttga
     1801 agctcttggg gtccacgttc ttgatgtcgc tgaacctgcc gcagttgatg aatgtggcca
     1861 ggaacaggaa ctggtacagg gtcttggtct tggtgaacct gctggtgtac tcgaagctgt
     1921 tcaggatctt ctcggtgatc tcccagatgc tctcgccctc ggacagcagg gccttcagca
     1981 tcttcttgct gtggctgttg cccttgtcgg cctcctcgct gctctcgaac tgcagctgca
     2041 ggctggacac gatgtcggtg atgtcgctct ggtgcttctg gccgttgtaa gggatgatgg
     2101 tgaactccca ggcggggatc agcttcttca ggctggcctc caggatggtg gccttctggg
     2161 tcttgtactt gaactgcagg ctcttgttca cgatgtcgaa gctcaggctg ttgctgatga
     2221 tggtgttgta gctcatgaag gtggccctct tgatggcggt gccgttgtgg gtgatcatcc
     2281 agcacaggta ggtcagctcg gcggcacagc tggcgatctt ctcgccgctg ggcctctcga
     2341 atctctccac gaactgccgc accagcacct tggggggggt cttgcacagg atgtcgaact
     2401 ggctcatcac cttcctcttc ttcttaggag ccattgtaca gctacccaga tcctcttctg
     2461 agatgagttt ttgttcacta ccaagcttgc atccccctcc gggatgacgg ctcacataac
     2521 cgcagcctcc tcccggatgt cgacttacat aaccgcaacc accgccgggg tgccgtgaaa
     2581 cgtaaccgca cccgcctccg ggatgcctgc ttacatatcc gcttccgctt cctctagagg
     2641 agccgctacc cttatcgtcg tcatccttgt agtcggatcc ggtgcggtag ttgatggaga
     2701 tcgggccgtg ccagtcccag aagttggcga agggcgtgac ggcgtacacc gtgatggtat
     2761 agtcaacacc aggtttcagg ccgctgatgg tagcagtgga tttgctgcca ggcacggtga
     2821 atttttggac agggctattg ccaccagttt cgccgtaggt gatgcggtag tagcgaatca
     2881 cctggtccct cctgctccag ctgatctgga tgctggttgg tgatgcttcc ttgacctcga
     2941 gcatgctagc tctaaattca gcgtctaggt ttacttcact aatttcacta gcgagctccg
     3001 gttcggtcag gcggcggcgc agttcttctt ccaggcggct cggactagta gtgctgaagt
     3061 aaacgatgat gatgatgagg atgatggcgc aaatcactcc caagatgatc atcatcaatt
     3121 gggaccctcc gcccttgggt tctgaggaga cagtgacctg ggtcccctga cccctcgtca
     3181 tatcccgggc ccagctccct tttgcacagt aatagacggc cgtgtcctcc ggtttcaggc
     3241 tgttcatctg cagatacagc gtgttcttgg cgttgtctct ggcgatggtg aatcggccct
     3301 tcacggactc tgtataggtt tcataattgc cactagcact aatagttgag acccattcga
     3361 gcccctttcc tggagcctgg cggacccagc tcataaaata tttactgaag gggaatccag
     3421 aggctgcaca ggagagtctt agagaccccc caggccgcac caagccgccc ccagactcct
     3481 ccagctgcac ctccatgcta gcataacttc gtataaagta tcctatacga agttatttgc
     3541 cttaacccag aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa
     3601 tgtatgctat acgaagttat gaattcgata tcaagcttat cgataatcaa cctctggatt
     3661 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3721 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3781 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3841 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3901 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3961 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4021 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     4081 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4141 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4201 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat
     4261 ctacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4381 ttacacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     4441 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     4501 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     4561 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     4621 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4681 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4741 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4801 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4861 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4921 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     4981 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     5041 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     5101 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     5161 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5221 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5281 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5341 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5401 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5461 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5521 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5581 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5641 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5701 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5761 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5821 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5881 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5941 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6001 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6061 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6121 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6181 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6241 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6301 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6361 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6601 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7141 tgt
//
