LOCUS       Exported                5958 bp ds-DNA     circular SYN 05-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-DIO-HA-BACE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 5, 2025 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /note="AAV2 ITR"
     promoter        175..622
                     /note="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    653..686
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    737..770
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(779..2311)
                     /note="BACE"
     CDS             complement(2150..2176)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     protein_bind    complement(2318..2351)
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    complement(2402..2435)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2460..3048
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2931..2942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     misc_feature    3078..3198
                     /note="SV40p"
     repeat_region   3221..3361
                     /note="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3436..3891
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4173..4277
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4278..5138
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5309..5897
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agggtaccgg atcctctaga gtcgactccg gaataacttc
      661 gtataggata ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc
      721 aatatgaaac ctcttaataa cttcgtatag catacattat acgaagttat ggcgcgccct
      781 acttcagcag ggagatgtca tcagcaaagt catcatgctg ctggcgcagg cagcggaggc
      841 agcgccactg acacaccatg aggcagagtg gcagcatgaa gagggcgcag atggcagcca
      901 tgacataggc tatggtcatg agggttgact catctgtctg tggaatgttg tagccacagt
      961 cttccatgtc caaggtgaca aaagggcctt ccaccgctgc cgtcctgaac tcatcgtgca
     1021 catggcaagc gctgacagca aagccaattc gttttcgggc ccgatcaaag acaacgtaga
     1081 agccctccat gataacagct cccataacag tgcccgtgga tgactgtgag atggcaaact
     1141 tgtaacagtc gtcttgggac gtggccacat cttccactgg ccgcaggtat tgctgcggaa
     1201 ggatggtgat gcggaaggac tggttggtaa cctcacccat taggtagagt gagatgactg
     1261 ggaaaatgtt ccaaggggtg gtgcctgctt gccagcacac cagctgctct cctagccaga
     1321 aaccatcagg gaacttctcc gtggaggagg ctgccttgat ggatttgact gcagcttcaa
     1381 acactttctt gggcaaacga aggttggtgg tgccactgtc cacaatgctc ttgtcatagt
     1441 tgtactcctt gcagtccatt ttcagatcct gtccattgat ctccacccgc acaatgatca
     1501 cctcataata ccactcccgc cggatgggtg tataccagag actgcctgtg tacagcgagt
     1561 ggtcgatacc tccaatgatc atgctccctc cgacagaggc cagcacttca gactggttga
     1621 gggggaagcc agcaccacaa agctgcaggg agaagaggtt gggaacgtgg gtctgcttta
     1681 ccagagagtc aaagaaaggc tccagggagt cgtcaggcct ggcaatctca gcataggcca
     1741 gccccaggat gccttcccag ttggagccgt tgatgaagaa cttgtctgat tcagtgatgg
     1801 cagcaatgtt ggcacgcaca gtgacgttgg ggccatgggg gatgcttacc aggtcggtgc
     1861 ccagctcccc ttcccacttg ccctgggtgt agggcacata cacacccttc cggaggtccc
     1921 ggtatgtgct ggacagctgc ctctggtagt agcgatgcag gaaggggtgg ggggcagcac
     1981 ccactgcaaa gttactgctg cctgtatcca ccaggatgtt gagcgtctgc ggggggctgc
     2041 ccacggtcat ctccacgtag tagccctgcc ccgacttgcc cctcaggttg tccaccatct
     2101 ccacaaagct gcccctccgg ccgggctcct cgggctcttc gtcggtctca gcgtaatctg
     2161 gtacatcgta agggtaccgg ggcagccgca gccccagggg ggcgcccccc aggccgctgc
     2221 gcaggggcag ccggatgccg tgctgggtgc cgtgggcagg cagcactccc gcgcccatcc
     2281 acagcaggag ccagggcagg gcttgggcca tgctagcata acttcgtata aagtatccta
     2341 tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag
     2401 aataacttcg tataatgtat gctatacgaa gttatgaatt cgatatcaag cttatcgata
     2461 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2521 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2581 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2641 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2701 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2761 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2821 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2881 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2941 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3001 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     3061 cgctgctcga gagatctact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3121 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3181 actcatcaat gtatcttaca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     3241 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     3301 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     3361 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     3421 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3481 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     3541 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3601 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     3661 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3721 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     3781 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     3841 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     3901 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     3961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     4081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5941 ggccttttgc tcacatgt
//
