LOCUS       pGL3_LRFN2              5643 bp ds-DNA     circular     17-MAY-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          2228..2249
                     /label="pGL3_5pseq_f"
                     /note="sequence: CGCTCTCCATCAAAACAAAACG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2341..2346
                     /label="NheI site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2979..2984
                     /label="XhoI site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3227..4879
                     /label="Luciferase"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5149..5150
                     /label="New Feature"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5149..5150
                     /label="SV40 enhancer WOULD BE HERE"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          complement(5206..5225)
                     /label="RVprimer4"
                     /note="sequence: GACGATAGTCATGCCCCGCG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
       61 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
      121 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
      181 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
      241 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
      301 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
      361 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
      421 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
      481 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
      541 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
      601 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
      661 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
      721 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
      781 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
      841 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
      901 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
      961 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     1021 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     1081 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     1141 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     1201 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     1261 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     1321 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     1381 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     1441 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     1501 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     1561 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG
     1621 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT
     1681 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA
     1741 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT
     1801 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
     1861 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT
     1921 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
     1981 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTG CCATTCGCCA
     2041 TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG
     2101 CCCAAGCTAC CATGATAAGT AAGTAATATT AAGGTACGGG AGGTACTTGG AGCGGCCGCA
     2161 ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC
     2221 TAACATACGC TCTCCATCAA AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC
     2281 CCAGTGCAAG TGCAGGTGCC AGAACATTTC TCTATCGATA GGTACCGAGC TCTTACGCGT
     2341 GCTAGCGCCC AGCTAGTAAG TTGGCagagc tgggatttaa atcaggcagt ttggctccag
     2401 ggcctccgca cctgacccct gggtcatgct gcctctcgga cactgccccg tcttcctgcc
     2461 cttcctgggc aactttgtga ggaaccctgc ctggtctccc caactcacct ttgctcccca
     2521 actcagagaa aatcataatg tgatgaagcc acatcctaat gtgatgaagc tgcggttggg
     2581 ctgtgctgct gatggaggtg taacaaggca aggctgattc ttggcgggcc actggaaccg
     2641 ggatgaaagg tcaggtctgg aacagttttg cactgtaatt ggcccatcag aaacaaggcc
     2701 agcaggaaaa gcctgctagg atgaagagca gaaaggcgga aggcggctcc tgattcctct
     2761 gctctgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta tgtgtgcgtg
     2821 tgtacgtctg tgtgtgtgtg ttggcggtag gaggagttgg ggtgacttaa ggagagccag
     2881 agccagctaa ctcaaaacag ccctggctgg caaggtcaag tttgcctttt ctaataacaa
     2941 taataaccag tatttgcctA GCACTTACTT GTCCTGGCCT CGAGATCTGC GATCTGCATC
     3001 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC
     3061 CCAGTTCCGC CCATTCTCCG CCCCATCGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG
     3121 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG
     3181 GCTTTTGCAA AAAGCTTGGC ATTCCGGTAC TGTTGGTAAA GCCACCATGG AAGACGCCAA
     3241 AAACATAAAG AAAGGCCCGG CGCCATTCTA TCCGCTGGAA GATGGAACCG CTGGAGAGCA
     3301 ACTGCATAAG GCTATGAAGA GATACGCCCT GGTTCCTGGA ACAATTGCTT TTACAGATGC
     3361 ACATATCGAG GTGGACATCA CTTACGCTGA GTACTTCGAA ATGTCCGTTC GGTTGGCAGA
     3421 AGCTATGAAA CGATATGGGC TGAATACAAA TCACAGAATC GTCGTATGCA GTGAAAACTC
     3481 TCTTCAATTC TTTATGCCGG TGTTGGGCGC GTTATTTATC GGAGTTGCAG TTGCGCCCGC
     3541 GAACGACATT TATAATGAAC GTGAATTGCT CAACAGTATG GGCATTTCGC AGCCTACCGT
     3601 GGTGTTCGTT TCCAAAAAGG GGTTGCAAAA AATTTTGAAC GTGCAAAAAA AGCTCCCAAT
     3661 CATCCAAAAA ATTATTATCA TGGATTCTAA AACGGATTAC CAGGGATTTC AGTCGATGTA
     3721 CACGTTCGTC ACATCTCATC TACCTCCCGG TTTTAATGAA TACGATTTTG TGCCAGAGTC
     3781 CTTCGATAGG GACAAGACAA TTGCACTGAT CATGAACTCC TCTGGATCTA CTGGTCTGCC
     3841 TAAAGGTGTC GCTCTGCCTC ATAGAACTGC CTGCGTGAGA TTCTCGCATG CCAGAGATCC
     3901 TATTTTTGGC AATCAAATCA TTCCGGATAC TGCGATTTTA AGTGTTGTTC CATTCCATCA
     3961 CGGTTTTGGA ATGTTTACTA CACTCGGATA TTTGATATGT GGATTTCGAG TCGTCTTAAT
     4021 GTATAGATTT GAAGAAGAGC TGTTTCTGAG GAGCCTTCAG GATTACAAGA TTCAAAGTGC
     4081 GCTGCTGGTG CCAACCCTAT TCTCCTTCTT CGCCAAAAGC ACTCTGATTG ACAAATACGA
     4141 TTTATCTAAT TTACACGAAA TTGCTTCTGG TGGCGCTCCC CTCTCTAAGG AAGTCGGGGA
     4201 AGCGGTTGCC AAGAGGTTCC ATCTGCCAGG TATCAGGCAA GGATATGGGC TCACTGAGAC
     4261 TACATCAGCT ATTCTGATTA CACCCGAGGG GGATGATAAA CCGGGCGCGG TCGGTAAAGT
     4321 TGTTCCATTT TTTGAAGCGA AGGTTGTGGA TCTGGATACC GGGAAAACGC TGGGCGTTAA
     4381 TCAAAGAGGC GAACTGTGTG TGAGAGGTCC TATGATTATG TCCGGTTATG TAAACAATCC
     4441 GGAAGCGACC AACGCCTTGA TTGACAAGGA TGGATGGCTA CATTCTGGAG ACATAGCTTA
     4501 CTGGGACGAA GACGAACACT TCTTCATCGT TGACCGCCTG AAGTCTCTGA TTAAGTACAA
     4561 AGGCTATCAG GTGGCTCCCG CTGAATTGGA ATCCATCTTG CTCCAACACC CCAACATCTT
     4621 CGACGCAGGT GTCGCAGGTC TTCCCGACGA TGACGCCGGT GAACTTCCCG CCGCCGTTGT
     4681 TGTTTTGGAG CACGGAAAGA CGATGACGGA AAAAGAGATC GTGGATTACG TCGCCAGTCA
     4741 AGTAACAACC GCGAAAAAGT TGCGCGGAGG AGTTGTGTTT GTGGACGAAG TACCGAAAGG
     4801 TCTTACCGGA AAACTCGACG CAAGAAAAAT CAGAGAGATC CTCATAAAGG CCAAGAAGGG
     4861 CGGAAAGATC GCCGTGTAAT TCTAGAGTCG GGGCGGCCGG CCGCTTCGAG CAGACATGAT
     4921 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
     4981 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
     5041 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
     5101 TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGATCTGG ATCCGTCGAC
     5161 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA
     5221 TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG
     5281 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
     5341 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
     5401 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     5461 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     5521 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     5581 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     5641 GGA
//