LOCUS       pGL3_IMPG1              5546 bp ds-DNA     circular     17-MAY-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name: 3-2_03.gbk
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(769..901)
                     /label="TcR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="11.167086481947942"
                     /fragment="True"
                     /other="CDS"
                     /translation="RRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAAL"
     CDS             923..991
                     /label="large T antigen"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.248587570621469"
                     /fragment="True"
                     /other="CDS"
                     /translation="YHICRGFTCFKKPPTPPPEPET*"
     polyA_signal    complement(1014..1144)
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.496"
                     /match_length="97.08029197080292"
                     /fragment="False"
                     /other="polyA signal"
     CDS             complement(1176..2828)
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     promoter        complement(2864..3060)
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3645..3736
                     /label="pause site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.925"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     polyA_signal    complement(3750..3798)
                     /label="polyA signal"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
     CDS             3857..3928
                     /label="lacZ"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="2.366863905325444"
                     /fragment="True"
                     /other="CDS"
                     /translation="AGVIAKRPAPIALPNSCAA*MANG"
     rep_origin      complement(3929..4384)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        4411..4515
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4516..5376
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgctgccg
      781 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
      841 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
      901 ggatccagat ccttatcgat tttaccacat ttgtagaggt tttacttgct ttaaaaaacc
      961 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1141 tctgctcgaa gcggccggcc gccccgactc tagaattaca cggcgatctt tccgcccttc
     1201 ttggccttta tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc
     1261 ggtacttcgt ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg
     1321 gcgacgtaat ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg
     1381 gcggcgggaa gttcaccggc gtcatcgtcg ggaagacctg cgacacctgc gtcgaagatg
     1441 ttggggtgtt ggagcaagat ggattccaat tcagcgggag ccacctgata gcctttgtac
     1501 ttaatcagag acttcaggcg gtcaacgatg aagaagtgtt cgtcttcgtc ccagtaagct
     1561 atgtctccag aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg
     1621 tttacataac cggacataat cataggacct ctcacacaca gttcgcctct ttgattaacg
     1681 cccagcgttt tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta
     1741 ccgaccgcgc ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca
     1801 gtgagcccat atccttgcct gatacctggc agatggaacc tcttggcaac cgcttccccg
     1861 acttccttag agaggggagc gccaccagaa gcaatttcgt gtaaattaga taaatcgtat
     1921 ttgtcaatca gagtgctttt ggcgaagaag gagaataggg ttggcaccag cagcgcactt
     1981 tgaatcttgt aatcctgaag gctcctcaga aacagctctt cttcaaatct atacattaag
     2041 acgactcgaa atccacatat caaatatccg agtgtagtaa acattccaaa accgtgatgg
     2101 aatggaacaa cacttaaaat cgcagtatcc ggaatgattt gattgccaaa aataggatct
     2161 ctggcatgcg agaatctcac gcaggcagtt ctatgaggca gagcgacacc tttaggcaga
     2221 ccagtagatc cagaggagtt catgatcagt gcaattgtct tgtccctatc gaaggactct
     2281 ggcacaaaat cgtattcatt aaaaccggga ggtagatgag atgtgacgaa cgtgtacatc
     2341 gactgaaatc cctggtaatc cgttttagaa tccatgataa taattttttg gatgattggg
     2401 agcttttttt gcacgttcaa aattttttgc aacccctttt tggaaacgaa caccacggta
     2461 ggctgcgaaa tgcccatact gttgagcaat tcacgttcat tataaatgtc gttcgcgggc
     2521 gcaactgcaa ctccgataaa taacgcgccc aacaccggca taaagaattg aagagagttt
     2581 tcactgcata cgacgattct gtgatttgta ttcagcccat atcgtttcat agcttctgcc
     2641 aaccgaacgg acatttcgaa gtactcagcg taagtgatgt ccacctcgat atgtgcatct
     2701 gtaaaagcaa ttgttccagg aaccagggcg tatctcttca tagccttatg cagttgctct
     2761 ccagcggttc catcttccag cggatagaat ggcgccgggc ctttctttat gtttttggcg
     2821 tcttccatgg tggctttacc aacagtaccg gaatgccaag ctttttgcaa aagcctaggc
     2881 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     2941 ctgcataaat aaaaaaaatt agtcagcgat ggggcggaga atgggcggaa ctgggcggag
     3001 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     3061 gatcgcagat cttaagatct caataattct ctccctcact cttttgcaat gttttgtttc
     3121 ctcaattcac actgagacct gcacgaagtt gtggaaactg ctctttccca gggagattaa
     3181 catgaattca tcttgaagtc ctaactgtgt tctggctatt taatgctgta catttataac
     3241 ctttgtcttc cttctttaag ctgcagtttc tttgtgttat cacagagata ggaaaactga
     3301 cacaggaaat gcataacaca tgttagactc caagacctct ctccatatgg gctcagttct
     3361 gatttcctct gtttcctata tggcactgag acaaatgcct ggacacaggg ttttttcttc
     3421 ctaaagactc acccagaagt tctgctatta atcatcaatc cgttcctcaa aataacttgg
     3481 catagctaaa cctagcaaag taaggaagat ctgacatatg gtgaagttct ccaacagcaa
     3541 ttgtgggatt acaagataac agccacaaat ctgtgtgcat ggctatttta catagctcaa
     3601 gacttcttga ggctagcacg cgtaagagct cggtacctat cgatagagaa atgttctggc
     3661 acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg ttttgttttg
     3721 atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca gatgtaatga
     3781 aaataaagat attttattgc ggccgctcca agtacctccc gtaccttaat attacttact
     3841 tatcatggta gcttgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3901 cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt gttaaaattc
     3961 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4021 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4081 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4141 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4201 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4261 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4321 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4381 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4441 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4501 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4561 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4621 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4681 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4741 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4801 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4861 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4921 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4981 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5041 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5101 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5161 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5221 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5281 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5341 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5521 cccgtagaaa agatcaaagg atcttc
//