LOCUS       pGL3_MECOM              5713 bp ds-DNA     circular     17-MAY-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    736..827
                     /label="pause site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.925"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     polyA_signal    complement(841..889)
                     /label="polyA signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
     CDS             948..1019
                     /label="lacZ"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="2.366863905325444"
                     /fragment="True"
                     /other="CDS"
                     /translation="AGVIAKRPAPIALPNSCAA*MANG"
     rep_origin      complement(1020..1475)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1502..1606
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1607..2467
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      2638..3226
                     /label="ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3406..3538)
                     /label="TcR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="11.167086481947942"
                     /fragment="True"
                     /other="CDS"
                     /translation="RRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAAL"
     CDS             3560..3628
                     /label="large T antigen"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.248587570621469"
                     /fragment="True"
                     /other="CDS"
                     /translation="YHICRGFTCFKKPPTPPPEPET*"
     polyA_signal    complement(3651..3781)
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.496"
                     /match_length="97.08029197080292"
                     /fragment="False"
                     /other="polyA signal"
     CDS             complement(3813..5465)
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     promoter        complement(5501..5697)
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 CAAAGCCCCA AGAATAAAGg ggcccttgtg tatggtgggc ttttcctcac tgttattctt
       61 ggtctcaact tgatgaagtg tattcgtgat atacacgaag cactgtgact cttgtgtctc
      121 tctttgtgag actgtccatg cctttgttcc tgctgtcagt cagttgttct ttctcttggg
      181 cagccacatt ggtaaccgca gtatctactt gcaaatagat ttctgtaaaa ccatattatc
      241 cttcaggcct ttactcctag cccccacccc tctttcctca acaatctggc cattcatgtg
      301 gcatagctcc cttgcttgct tctttgccat atgtctagtg gtttgtgttt cagggaggct
      361 gtgtgataca ctggaatgaa aactgggctg ggtgccagga accttgaatt tttgcacaag
      421 tcagacttgg gaacttggaa aagtcatttg actttaccct ctgaagtctc actcaggtct
      481 cttgtagctg taactctatg agtcttcgaa ttctgtctct tacacctgct ctttagttct
      541 tttcatcttt atggacatgt tctatattgt catggttttg gaggttttaa tttattgttg
      601 ttgttgagaa aagcccacca acaacaataa acaaaacctc caaaataatt acttttaatg
      661 gcaaaactgc aattactttt acACCAACCT AATATATTGG GGgctagcac gcgtaagagc
      721 tcggtaccta tcgatagaga aatgttctgg cacctgcact tgcactgggg acagcctatt
      781 ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt
      841 cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg cggccgctcc
      901 aagtacctcc cgtaccttaa tattacttac ttatcatggt agcttgggct ggcgtaatag
      961 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca
     1021 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     1081 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     1141 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     1201 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     1261 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     1321 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     1381 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     1441 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     1501 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1561 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1621 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1681 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1741 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1801 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1861 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1921 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1981 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2041 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2101 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2161 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2221 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2281 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2341 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2401 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2461 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2521 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2581 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2641 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2701 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2761 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2821 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2881 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2941 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3001 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3061 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3121 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3181 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3241 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3301 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3361 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gctgccggca
     3421 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
     3481 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgga
     3541 tccagatcct tatcgatttt accacatttg tagaggtttt acttgcttta aaaaacctcc
     3601 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3781 gctcgaagcg gccggccgcc ccgactctag aattacacgg cgatctttcc gcccttcttg
     3841 gcctttatga ggatctctct gatttttctt gcgtcgagtt ttccggtaag acctttcggt
     3901 acttcgtcca caaacacaac tcctccgcgc aactttttcg cggttgttac ttgactggcg
     3961 acgtaatcca cgatctcttt ttccgtcatc gtctttccgt gctccaaaac aacaacggcg
     4021 gcgggaagtt caccggcgtc atcgtcggga agacctgcga cacctgcgtc gaagatgttg
     4081 gggtgttgga gcaagatgga ttccaattca gcgggagcca cctgatagcc tttgtactta
     4141 atcagagact tcaggcggtc aacgatgaag aagtgttcgt cttcgtccca gtaagctatg
     4201 tctccagaat gtagccatcc atccttgtca atcaaggcgt tggtcgcttc cggattgttt
     4261 acataaccgg acataatcat aggacctctc acacacagtt cgcctctttg attaacgccc
     4321 agcgttttcc cggtatccag atccacaacc ttcgcttcaa aaaatggaac aactttaccg
     4381 accgcgcccg gtttatcatc cccctcgggt gtaatcagaa tagctgatgt agtctcagtg
     4441 agcccatatc cttgcctgat acctggcaga tggaacctct tggcaaccgc ttccccgact
     4501 tccttagaga ggggagcgcc accagaagca atttcgtgta aattagataa atcgtatttg
     4561 tcaatcagag tgcttttggc gaagaaggag aatagggttg gcaccagcag cgcactttga
     4621 atcttgtaat cctgaaggct cctcagaaac agctcttctt caaatctata cattaagacg
     4681 actcgaaatc cacatatcaa atatccgagt gtagtaaaca ttccaaaacc gtgatggaat
     4741 ggaacaacac ttaaaatcgc agtatccgga atgatttgat tgccaaaaat aggatctctg
     4801 gcatgcgaga atctcacgca ggcagttcta tgaggcagag cgacaccttt aggcagacca
     4861 gtagatccag aggagttcat gatcagtgca attgtcttgt ccctatcgaa ggactctggc
     4921 acaaaatcgt attcattaaa accgggaggt agatgagatg tgacgaacgt gtacatcgac
     4981 tgaaatccct ggtaatccgt tttagaatcc atgataataa ttttttggat gattgggagc
     5041 tttttttgca cgttcaaaat tttttgcaac ccctttttgg aaacgaacac cacggtaggc
     5101 tgcgaaatgc ccatactgtt gagcaattca cgttcattat aaatgtcgtt cgcgggcgca
     5161 actgcaactc cgataaataa cgcgcccaac accggcataa agaattgaag agagttttca
     5221 ctgcatacga cgattctgtg atttgtattc agcccatatc gtttcatagc ttctgccaac
     5281 cgaacggaca tttcgaagta ctcagcgtaa gtgatgtcca cctcgatatg tgcatctgta
     5341 aaagcaattg ttccaggaac cagggcgtat ctcttcatag ccttatgcag ttgctctcca
     5401 gcggttccat cttccagcgg atagaatggc gccgggcctt tctttatgtt tttggcgtct
     5461 tccatggtgg ctttaccaac agtaccggaa tgccaagctt tttgcaaaag cctaggcctc
     5521 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     5581 cataaataaa aaaaattagt cagcgatggg gcggagaatg ggcggaactg ggcggagtta
     5641 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcagat
     5701 cgcagatctc gag
//