LOCUS       KRD22_HBA1(HBA1reg-HBB) 6134 bp ds-DNA     circular     10-JAN-2025
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:Right_AAV2 ITR, AmpR, Amplicon for
            qPCR, Amplicon for qPCR, HBA1_5'UTR, HBA1_3'UTR"
FEATURES             Location/Qualifiers
     LTR             1..130
                     /label="Left-ITR_AAV"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    68..130
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    139..1084
                     /label="LHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1019..1084
                     /label="HBA1_5'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1085..1176
                     /label="HBB_Exon_1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1307..1529
                     /label="HBB_Exon_2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2380..2508
                     /label="HBB_Exon_3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2506..2508
                     /label="STOP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2509..2606
                     /label="HBA1_3'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2509..3387
                     /label="RHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3397..3459
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   3397..3537
                     /label="Right_AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      3612..4067
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        4349..4453
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             4454..4522
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             4523..5314
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      5485..6073
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCgc tccagccggt tccagctatt gctttgttta cctgtttaac
      181 cagtatttac ctagcaagtc ttccatcaga tagcatttgg agagctgggg gtgtcacagt
      241 gaaccacgac ctctaggcca gtgggagagt cagtcacaca aactgtgagt ccatgacttg
      301 gggcttagcc agcacccacc accccacgcg ccaccccaca accccgggta gaggagtctg
      361 aatctggagc cgcccccagc ccagccccgt gctttttgcg tcctggtgtt tattccttcc
      421 cggtgcctgt cactcaagca cactagtgac tatcgccaga gggaaaggga gctgcaggaa
      481 gcgaggctgg agagcaggag gggctctgcg cagaaattct tttgagttcc tatgggccag
      541 ggcgtccggg tgcgcgcatt cctctccgcc ccaggattgg gcgaagcctc ccggctcgca
      601 ctcgctcgcc cgtgtgttcc ccgatcccgc tggagtcgat gcgcgtccag cgcgtgccag
      661 gccggggcgg gggtgcgggc tgactttctc cctcgctagg gacgctccgg cgcccgaaag
      721 gaaagggtgg cgctgcgctc cggggtgcac gagccgacag cgcccgaccc caacgggccg
      781 gccccgccag cgccgctacc gccctgcccc cgggcgagcg ggatgggcgg gagtggagtg
      841 gcgggtggag ggtggagacg tcctggcccc cgccccgcgt gcacccccag gggaggccga
      901 gcccgccgcc cggccccgcg caggccccgc ccgggactcc cctgcggtcc aggccgcgcc
      961 ccgggctccg cgccagccaa tgagcgccgc ccggccgggc gtgcccccgc gccccaagca
     1021 taaaccctgg cgcgctcgcg gcccggcact cttctggtcc ccacagactc agagagaacc
     1081 caccATGGTG CATCTGACTC CTGAGGAGAA GTCTGCCGTT ACTGCCCTGT GGGGCAAGGT
     1141 GAACGTGGAT GAAGTTGGTG GTGAGGCCCT GGGCAGgttg gtatcaaggt tacaagacag
     1201 gtttaaggag accaatagaa actgggcatg tggagacaga gaagactctt gggtttctga
     1261 taggcactga ctctctctgc ctattggtct attttcccac ccttagGCTG CTGGTGGTCT
     1321 ACCCTTGGAC CCAGAGGTTC TTTGAGTCCT TTGGGGATCT GTCCACTCCT GATGCTGTTA
     1381 TGGGCAACCC TAAGGTGAAG GCTCATGGCA AGAAAGTGCT CGGTGCCTTT AGTGATGGCC
     1441 TGGCTCACCT GGACAACCTC AAGGGCACCT TTGCCACACT GAGTGAGCTG CACTGTGACA
     1501 AGCTGCACGT GGATCCTGAG AACTTCAGGg tgagtctatg ggacgcttga tgttttcttt
     1561 ccccttcttt tctatggtta agttcatgtc ataggaaggg gataagtaac agggtacagt
     1621 ttagaatggg aaacagacga atgattgcat cagtgtggaa gtctcaggat cgttttagtt
     1681 tcttttattt gctgttcata acaattgttt tcttttgttt aattcttgct ttcttttttt
     1741 ttcttctccg caatttttac tattatactt aatgccttaa cattgtgtat aacaaaagga
     1801 aatatctctg agatacatta agtaacttaa aaaaaaactt tacacagtct gcctagtaca
     1861 ttactatttg gaatatatgt gtgcttattt gcatattcat aatctcccta ctttattttc
     1921 ttttattttt aattgataca taatcattat acatatttat gggttaaagt gtaatgtttt
     1981 aatatgtgta cacatattga ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg
     2041 ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt
     2101 tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac
     2161 agtgataatt tctgggttaa ggcaatagca atatctctgc atataaatat ttctgcatat
     2221 aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc cagctaccat
     2281 tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag ctaggccctt
     2341 ttgctaatca tgttcatacc tcttatcttc ctcccacagC TCCTGGGCAA CGTGCTGGTC
     2401 TGTGTGCTGG CCCATCACTT TGGCAAAGAA TTCACCCCAC CAGTGCAGGC TGCCTATCAG
     2461 AAAGTGGTGG CTGGTGTGGC TAATGCCCTG GCCCACAAGT ATCACTAAtg gccatgcttc
     2521 ttgccccttg ggcctccccc cagcccctcc tccccttcct gcacccgtac ccccgtggtc
     2581 tttgaataaa gtctgagtgg gcggcagcct gtgtgtgcct gagttttttc cctcagcaaa
     2641 cgtgccaggc atgggcgtgg acagcagctg ggacacacat ggctagaacc tctctgcagc
     2701 tggatagggt aggaaaaggc aggggcggga ggaggggatg gaggagggaa agtggagcca
     2761 ccgcgaagtc cagctggaaa aacgctggac cctagagtgc tttgaggatg catttgctct
     2821 ttcccgagtt ttattcccag acttttcaga ttcaatgcag gtttgctgaa ataatgaatt
     2881 tatccatctt tacgtttctg ggcactctgt gccaagaact ggctggcttt ctgcctggga
     2941 cgtcactggt ttcccagagg tcctcccaca tatgggtggt gggtaggtca gagaagtccc
     3001 actccagcat ggctgcattg atcccccatc gttcccacta gtctccgtaa aacctcccag
     3061 atacaggcac agtctagatg aaatcagggg tgcggggtgc aactgcaggc cccaggcaat
     3121 tcaatagggg ctctactttc acccccaggt caccccagaa tgctcacaca ccagacactg
     3181 acgccctggg gctgtcaaga tcaggcgttt gtctctgggc ccagctcagg gcccagctca
     3241 gcacccactc agctcccctg aggctgggga gcctgtccca ttgcgactgg agaggagagc
     3301 ggggccacag aggcctggct agaaggtccc ttctccctgg tgtgtgtttt ctctctgctg
     3361 agcaggcttg cagtgcctgg ggtatcatGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG
     3421 CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC
     3481 GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC
     3541 GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA
     3601 GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
     3661 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
     3721 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
     3781 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC
     3841 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
     3901 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC
     3961 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
     4021 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT
     4081 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC
     4141 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT
     4201 CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA
     4261 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC
     4321 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
     4381 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
     4441 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
     4501 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
     4561 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
     4621 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
     4681 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
     4741 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
     4801 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     4861 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     4921 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     4981 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     5041 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     5101 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     5161 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     5221 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     5281 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT
     5341 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
     5401 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
     5461 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     5521 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     5581 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT
     5641 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     5701 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     5761 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     5821 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     5881 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     5941 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     6001 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     6061 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     6121 TTTTGCTCAC ATGT
//