LOCUS       MKC91_tEPOR-UbC-GFP-BGH 7042 bp ds-DNA     circular     10-JAN-2025
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:EPOR_3'UTR, Right_AAV2 ITR, AmpR,
            Amplicon for qPCR, Amplicon for qPCR"
FEATURES             Location/Qualifiers
     LTR             1..130
                     /label="Left-ITR_AAV"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    68..130
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    139..1103
                     /label="LHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    521..608
                     /label="EPOR_Exon_7"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    704..1102
                     /label="EPOR_Exon_8"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1104..1104
                     /label="W439X_tEPOR_mutation"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     polyA_signal    1137..1361
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#666699"
                     /ApEinfo_fwdcolor="#666699"
                     /note="note: bovine growth hormone polyadenylation signal"
     misc_feature    1409..2620
                     /label="UbC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: Ubc"
     CDS             2654..3334
                     /label="TurboGFP"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 transl_table: 1 translation: MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFARSRV*"
     misc_feature    3335..3544
                     /label="EPOR_Exon_8"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3335..4296
                     /label="RHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3545..4296
                     /label="EPOR_3'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4305..4367
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   4305..4445
                     /label="Right_AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      4520..4975
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        5257..5361
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             5362..5430
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             5431..6222
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      6393..6981
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCga gatgggggtc tcactatgtt tcctaggctg gtcttgaact
      181 cctgggttca aatgatcctc ccacctcagc ctcccaaagt actgggatta tagtgctggt
      241 gtaaaccact gcacctggcc atggccagga ttaaagggag aatgaccaag gtatattgaa
      301 ctcctatgca cccttcaata ccctgttcca tttacccttt tgtagggcct tgctgatgct
      361 tcagccaaaa cccctgtccc ctggccctga tgtactcctc tgcctccatt gtgatcacag
      421 ggaccaagtg tatctgtgcc tctatgactg ggagtggagg gggaattggt gagtattcaa
      481 tgagtcatat ctatgtaact atttatattg gcttcaacag GGCTCTGAAG CAGAAGATCT
      541 GGCCTGGCAT CCCGAGCCCA GAGAGCGAGT TTGAAGGCCT CTTCACCACC CACAAGGGTA
      601 ACTTCCAGgt aggtggcctg gttgtcccct cagtgcctgg gcttccctgc ttcttgcagc
      661 caaactgcag gcctctctga gcaggttggt gctatttctt cagCTGTGGC TGTACCAGAA
      721 TGATGGCTGC CTGTGGTGGA GCCCCTGCAC CCCCTTCACG GAGGACCCAC CTGCTTCCCT
      781 GGAAGTCCTC TCAGAGCGCT GCTGGGGGAC GATGCAGGCA GTGGAGCCGG GGACAGATGA
      841 TGAGGGCCCC CTGCTGGAGC CAGTGGGCAG TGAGCATGCC CAGGATACCT ATCTGGTGCT
      901 GGACAAATGG TTGCTGCCCC GGAACCCGCC CAGTGAGGAC CTCCCAGGGC CTGGTGGCAG
      961 TGTGGACATA GTGGCCATGG ATGAAGGCTC AGAAGCATCC TCCTGCTCAT CTGCTTTGGC
     1021 CTCGAAGCCC AGCCCAGAGG GAGCCTCTGC TGCCAGCTTT GAGTACACTA TCCTGGACCC
     1081 CAGCTCCCAG CTCTTGCGTC CATAGctcga gggcgcgccC GCTGATCAGC CTCGACCTGT
     1141 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA
     1201 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG
     1261 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA
     1321 AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC
     1381 GTTTCGCGCC attaccctgt tatccctaGG CCTCCGCGCC GGGTTTTGGC GCCTCCCGCG
     1441 GGCGCCCCCC TCCTCACGGC GAGCGCTGCC ACGTCAGACG AAGGGCGCAG CGAGCGTCCT
     1501 GATCCTTCCG CCCGGACGCT CAGGACAGCG GCCCGCTGCT CATAAGACTC GGCCTTAGAA
     1561 CCCCAGTATC AGCAGAAGGA CATTTTAGGA CGGGACTTGG GTGACTCTAG GGCACTGGTT
     1621 TTCTTTCCAG AGAGCGGAAC AGGCGAGGAA AAGTAGTCCC TTCTCGGCGA TTCTGCGGAG
     1681 GGATCTCCGT GGGGCGGTGA ACGCCGATGA TTATATAAGG ACGCGCCGGG TGTGGCACAG
     1741 CTAGTTCCGT CGCAGCCGGG ATTTGGGTCG CGGTTCTTGT TTGTGGATCG CTGTGATCGT
     1801 CACTTGGTGA GTAGCGGGCT GCTGGGCTGG CCGGGGCTTT CGTGGCCGCC GGGCCGCTCG
     1861 GTGGGACGGA AGCGTGTGGA GAGACCGCCA AGGGCTGTAG TCTGGGTCCG CGAGCAAGGT
     1921 TGCCCTGAAC TGGGGGTTGG GGGGAGCGCA GCAAAATGGC GGCTGTTCCC GAGTCTTGAA
     1981 TGGAAGACGC TTGTGAGGCG GGCTGTGAGG TCGTTGAAAC AAGGTGGGGG GCATGGTGGG
     2041 CGGCAAGAAC CCAAGGTCTT GAGGCCTTCG CTAATGCGGG AAAGCTCTTA TTCGGGTGAG
     2101 ATGGGCTGGG GCACCATCTG GGGACCCTGA CGTGAAGTTT GTCACTGACT GGAGAACTCG
     2161 GTTTGTCGTC TGTTGCGGGG GCGGCAGTTA TGGCGGTGCC GTTGGGCAGT GCACCCGTAC
     2221 CTTTGGGAGC GCGCGCCCTC GTCGTGTCGT GACGTCACCC GTTCTGTTGG CTTATAATGC
     2281 AGGGTGGGGC CACCTGCCGG TAGGTGTGCG GTAGGCTTTT CTCCGTCGCA GGACGCAGGG
     2341 TTCGGGCCTA GGGTAGGCTC TCCTGAATCG ACAGGCGCCG GACCTCTGGT GAGGGGAGGG
     2401 ATAAGTGAGG CGTCAGTTTC TTTGGTCGGT TTTATGTACC TATCTTCTTA AGTAGCTGAA
     2461 GCTCCGGTTT TGAACTATGC GCTCGGGGTT GGCGAGTGTG TTTTGTGAAG TTTTTTAGGC
     2521 ACCTTTTGAA ATGTAATCAT TTGGGTCAAT ATGTAATTTT CAGTGTTAGA CTAGTAAATT
     2581 GTCCGCTAAA TTCTGGCCGT TTTTGGCTTT TTTGTTAGAC ggtaccgagc tcttcgaagg
     2641 atccatcgcc accATGCCCG CCATGAAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG
     2701 CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA
     2761 CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT
     2821 GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA
     2881 CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT
     2941 GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT
     3001 GGTGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA
     3061 CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGTG CTGGTGGGCA GCTTCGCCCG
     3121 CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTTCGTG GTGGACAGCC ACATGCACTT
     3181 CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG
     3241 CGTGGAGGAG CTGCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA
     3301 GACCCCCATC GCCTTCGCCA GATCTCGAGT CTAGACACTG TGCCCTGAGC TGCCCCCTAC
     3361 CCCACCCCAC CTAAAGTACC TGTACCTTGT GGTATCTGAC TCTGGCATCT CAACTGACTA
     3421 CAGCTCAGGG GACTCCCAGG GAGCCCAAGG GGGCTTATCC GATGGCCCCT ACTCCAACCC
     3481 TTATGAGAAC AGCCTTATCC CAGCCGCTGA GCCTCTGCCC CCCAGCTATG TGGCTTGCTC
     3541 TTAGGACACC AGGCTGCAGA TGATCAGGGA TCCAATATGA CTCAGAGAAC CAGTGCAGAC
     3601 TCAAGACTTA TGGAACAGGG ATGGCGAGGC CTCTCTCAGG AGCAGGGGCA TTGCTGATTT
     3661 TGTCTGCCCA ATCCATCCTG CTCAGGAAAC CACAACCTTG CAGTATTTTT AAATATGTAT
     3721 AGTTTTTTTT TGTATCTATA TATATATATA CACATATGTA TGTAAGTTTT TCTACCATGA
     3781 TTTCTACAAA CACCCTTTAA GTCCCATCTT CCCCTGGGCA TAGGCCATAG GGATAGAAGT
     3841 TAAAGTTCTT GAGCTTATTC AGAAGCTGGA TCTGCAATCT GAATGCTACT CATAACATAA
     3901 CAAAATAGTA TGTTAAACAG CTCTTAAATC TTACTGGCTT ACCACATTAA ATGATTTCTC
     3961 TCTCCTAACT CAGCTCAAAT GGGCAGCCAT CCATGGGATG AGTCAGAGGT TCAGACTCTT
     4021 CCAGTCTGTA GCTCTACCTT CTCTTAGGGT ACTTAGATGG ATCCCCTGTT CTACAAACTG
     4081 CCAGTCAGCA AGGGAAGAAA AAGGGCAGCA ATGACCCTCA ATGGGCCATT TGAGGGATCT
     4141 GGCCTGGAAA TGGGCTTCCT CTCTTCTTCT CACACCTCAC TGGCTGGAAA CAGTCACATG
     4201 ACCCCAGTCA CATGAAAGGC CAGGAAACTT AGTTTAGCTG TACACCCAGG AAGGGCAAAG
     4261 CTGTTTAAGG GCCACTAGCT AGTCTCTGCC ACTAATGCGG CCGCAGGAAC CCCTAGTGAT
     4321 GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT
     4381 CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT
     4441 GCAGGGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT
     4501 ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG
     4561 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC
     4621 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC
     4681 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT
     4741 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG
     4801 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG
     4861 GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG
     4921 CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTTATGG
     4981 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA
     5041 ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT
     5101 GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG
     5161 AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT
     5221 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT
     5281 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA
     5341 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT
     5401 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT
     5461 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG
     5521 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG
     5581 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA
     5641 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT
     5701 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC
     5761 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG
     5821 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC
     5881 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT
     5941 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA
     6001 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT
     6061 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC
     6121 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA
     6181 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC
     6241 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG
     6301 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG
     6361 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC
     6421 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG
     6481 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
     6541 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC
     6601 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC
     6661 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT
     6721 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT
     6781 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
     6841 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT
     6901 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
     6961 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
     7021 TGCTGGCCTT TTGCTCACAT GT
//