LOCUS       MKC62_HBB(HBA1reg-HBA1- 6278 bp ds-DNA     circular     10-JAN-2025
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:Amplicon for qPCR, Amplicon for qPCR,
            AmpR, Right_AAV2 ITR, HBA1_5'UTR, HBA1_3'UTR"
FEATURES             Location/Qualifiers
     LTR             1..130
                     /label="Left-ITR_AAV"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    68..130
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    139..988
                     /label="LHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     source          889..988
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: organism: unspecified"
     misc_feature    989..1054
                     /label="HBA1_5'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1055..1149
                     /label="HBA1_Exon_1"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1267..1471
                     /label="HBA1_Exon_2"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1445..1445
                     /label="dbSNP_rs17407508 (T = 2%)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1621..1746
                     /label="HBA1_Exon_3"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     CDS             1747..1800
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. product: 2A peptide from Thosea asigna virus capsid protein protein_id: transl_table: 1 translation: EGRGSLLTCGDVEENPGP"
     CDS             1801..1803
                     /label="mEYFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002) transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     CDS             1804..1806
                     /label="mEYFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002) transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     CDS             1807..2520
                     /label="mEYFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002) transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    2521..2631
                     /label="HBA1_3'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     source          2632..3531
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: organism: unspecified"
     misc_feature    2632..3531
                     /label="RHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2653..2875
                     /label="HBB_Exon_2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: HBB_Exon_2"
     misc_feature    3541..3603
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   3541..3681
                     /label="Right_AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      3756..4211
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        4493..4597
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             4598..4666
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             4667..5458
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      5629..6217
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAT TAGTCCAGGC AGAAACAGTT AGATGTCCCC AGTTAACCTC
      181 CTATTTGACA CCACTGATTA CCCCATTGAT AGTCACACTT TGGGTTGTAA GTGACTTTTT
      241 ATTTATTTGT ATTTTTGACT GCATTAAGAG GTCTCTAGTT TTTTATCTCT TGTTTCCCAA
      301 AACCTAATAA GTAACTAATG CACAGAGCAC ATTGATTTGT ATTTATTCTA TTTTTAGACA
      361 TAATTTATTA GCATGCATGA GCAAATTAAG AAAAACAACA ACAAATGAAT GCATATATAT
      421 GTATATGTAT GTGTGTATAT ATACACACAT ATATATATAT ATTTTTTCTT TTCTTACCAG
      481 AAGGTTTTAA TCCAAATAAG GAGAAGATAT GCTTAGAACC GAGGTAGAGT TTTCATCCAT
      541 TCTGTCCTGT AAGTATTTTG CATATTCTGG AGACGCAGGA AGAGATCCAT CTACATATCC
      601 CAAAGCTGAA TTATGGTAGA CAAAACTCTT CCACTTTTAG TGCATCAACT TCTTATTTGT
      661 GTAATAAGAA AATTGGGAAA ACGATCTTCA ATATGCTTAC CAAGCTGTGA TTCCAAATAT
      721 TACGTAAATA CACTTGCAAA GGAGGATGTT TTTAGTAGCA ATTTGTACTG ATGGTATGGG
      781 GCCAAGAGAT ATATCTTAGA GGGAGGGCTG AGGGTTTGAA GTCCAACTCC TAAGCCAGTG
      841 CCAGAAGAGC CAAGGACAGG TACGGCTGTC ATCACTTAGA CCTCACCCtg tggagccaca
      901 ccctagggtt ggccaatcta ctcccaggag cagggagggc aggagccagg gctgggcata
      961 aaagtcaggg cagagccatc tattgcttca taaaccctgg cgcgctcgcg gcccggcact
     1021 cttctggtcc ccacagactc agagagaacc caccatggtg ctgtctcctg ccgacaagac
     1081 caacgtcaag gccgcctggg gtaaggtcgg cgcgcacgct ggcgagtatg gtgcggaggc
     1141 cctggagagg tgaggctccc tcccctgctc cgacccgggc tcctcgcccg cccggaccca
     1201 caggccaccc tcaaccgtcc tggccccgga cccaaacccc acccctcact ctgcttctcc
     1261 ccgcaggatg ttcctgtcct tccccaccac caagacctac ttcccgcact tcgacctgag
     1321 ccacggctct gcccaggtta agggccacgg caagaaggtg gccgacgcgc tgaccaacgc
     1381 cgtggcgcac gtggacgaca tgcccaacgc gctgtccgcc ctgagcgacc tgcacgcgca
     1441 caagcttcgg gtggacccgg tcaacttcaa ggtgagcggc gggccgggag cgatctgggt
     1501 cgaggggcga gatggcgcct tcctcgcagg gcagaggatc acgcgggttg cgggaggtgt
     1561 agcgcaggcg gcggctgcgg gcctgggccc tcggccccac tgaccctctt ctctgcacag
     1621 ctcctaagcc actgcctgct ggtgaccctg gccgcccacc tccccgccga gttcacccct
     1681 gcggtgcacg cctccctgga caagttcctg gcttctgtga gcaccgtgct gacctccaaa
     1741 taccgtgagg gcaggggcag cctgctgacc tgcggcgacg tggaggagaa ccccggcccc
     1801 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1861 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1921 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1981 ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga ccacatgaag
     2041 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2101 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2161 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2221 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2281 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2341 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2401 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2461 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     2521 gctggagcct cggtggccat gcttcttgcc ccttgggcct ccccccagcc cctcctcccc
     2581 ttcctgcacc cgtacccccg tggtctttga ataaagtctg agtgggcggc aggtctattt
     2641 tcccaccctt aggctgctgg tggtctaccc ttggacccag aggttctttg agtcctttgg
     2701 ggatctgtcc actcctgatg ctgttatggg caaccctaag gtgaaggctc atggcaagaa
     2761 agtgctcggt gcctttagtg atggcctggc tcacctggac aacctcaagg gcacctttgc
     2821 cacactgagt gagctgcact gtgacaagct gcacgtggat cctgagaact tcagggtgag
     2881 tctatgggac gcttgatgtt ttctttcccc ttcttttcta tggttaagtt catgtcatag
     2941 gaaggggata agtaacaggg tacagtttag aatgggaaac agacgaatga ttgcatcagt
     3001 gtggaagtct caggatcgtt ttagtttctt ttatttgctg ttcataacaa ttgttttctt
     3061 ttgtttaatt cttgctttct ttttttttct tctccgcaat ttttactatt atacttaatg
     3121 ccttaacatt gtgtataaca aaaggaaata tctctgagat acattaagta acttaaaaaa
     3181 aaactttaca cagtctgcct agtacattac tatttggaat atatgtgtgc ttatttgcat
     3241 attcataatc tccctacttt attttctttt atttttaatt gatacataat cattatacat
     3301 atttatgggt taaagtgtaa tgttttaata tgtgtacaca tattgaccaa atcagggtaa
     3361 ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt
     3421 atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc
     3481 ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca atGCGGCCGC
     3541 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG
     3601 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC
     3661 GAGCGCGCAG CTGCCTGCAG GGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG
     3721 GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG
     3781 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC
     3841 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
     3901 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA
     3961 AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG
     4021 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC
     4081 ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA
     4141 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
     4201 GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA
     4261 GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC
     4321 CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC
     4381 ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT
     4441 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
     4501 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
     4561 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
     4621 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT
     4681 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA
     4741 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG
     4801 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA
     4861 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
     4921 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG
     4981 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC
     5041 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA
     5101 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT
     5161 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
     5221 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT
     5281 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG
     5341 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT
     5401 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT
     5461 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA
     5521 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
     5581 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     5641 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     5701 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     5761 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     5821 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     5881 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     5941 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     6001 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     6061 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     6121 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     6181 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     6241 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGT
//