LOCUS       MKC83_HBB(HBBreg-HBA1)  5512 bp ds-DNA     circular     10-JAN-2025
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:HBB_5'UTR, Amplicon for qPCR, Amplicon
            for qPCR, AmpR, Right_AAV2 ITR, HBB_3'UTR"
FEATURES             Location/Qualifiers
     LTR             1..130
                     /label="Left-ITR_AAV"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    68..130
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    139..1038
                     /label="LHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     source          889..1038
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: organism: unspecified"
     misc_feature    989..1038
                     /label="HBB_5'UTR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: HBB_5'UTR"
     misc_feature    1039..1133
                     /label="HBA1_Exon_1"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1251..1455
                     /label="HBA1_Exon_2"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1605..1730
                     /label="HBA1_Exon_3"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1731..1733
                     /label="STOP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     source          1734..1865
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: organism: unspecified"
     misc_feature    1734..1865
                     /label="HBB_3'UTR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: HBB_3'UTR"
     misc_feature    1866..2765
                     /label="RHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     source          1866..2765
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: organism: unspecified"
     misc_feature    1887..2109
                     /label="HBB_Exon_2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: HBB_Exon_2"
     misc_feature    2775..2837
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   2775..2915
                     /label="Right_AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      2990..3445
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        3727..3831
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             3832..3900
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             3901..4692
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      4863..5451
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAT TAGTCCAGGC AGAAACAGTT AGATGTCCCC AGTTAACCTC
      181 CTATTTGACA CCACTGATTA CCCCATTGAT AGTCACACTT TGGGTTGTAA GTGACTTTTT
      241 ATTTATTTGT ATTTTTGACT GCATTAAGAG GTCTCTAGTT TTTTATCTCT TGTTTCCCAA
      301 AACCTAATAA GTAACTAATG CACAGAGCAC ATTGATTTGT ATTTATTCTA TTTTTAGACA
      361 TAATTTATTA GCATGCATGA GCAAATTAAG AAAAACAACA ACAAATGAAT GCATATATAT
      421 GTATATGTAT GTGTGTATAT ATACACACAT ATATATATAT ATTTTTTCTT TTCTTACCAG
      481 AAGGTTTTAA TCCAAATAAG GAGAAGATAT GCTTAGAACC GAGGTAGAGT TTTCATCCAT
      541 TCTGTCCTGT AAGTATTTTG CATATTCTGG AGACGCAGGA AGAGATCCAT CTACATATCC
      601 CAAAGCTGAA TTATGGTAGA CAAAACTCTT CCACTTTTAG TGCATCAACT TCTTATTTGT
      661 GTAATAAGAA AATTGGGAAA ACGATCTTCA ATATGCTTAC CAAGCTGTGA TTCCAAATAT
      721 TACGTAAATA CACTTGCAAA GGAGGATGTT TTTAGTAGCA ATTTGTACTG ATGGTATGGG
      781 GCCAAGAGAT ATATCTTAGA GGGAGGGCTG AGGGTTTGAA GTCCAACTCC TAAGCCAGTG
      841 CCAGAAGAGC CAAGGACAGG TACGGCTGTC ATCACTTAGA CCTCACCCtg tggagccaca
      901 ccctagggtt ggccaatcta ctcccaggag cagggagggc aggagccagg gctgggcata
      961 aaagtcaggg cagagccatc tattgcttac atttgcttct gacacaactg tgttcactag
     1021 caacctcaaa cagacaccat ggtgctgtct cctgccgaca agaccaacgt caaggccgcc
     1081 tggggtaagg tcggcgcgca cgctggcgag tatggtgcgg aggccctgga gaggtgaggc
     1141 tccctcccct gctccgaccc gggctcctcg cccgcccgga cccacaggcc accctcaacc
     1201 gtcctggccc cggacccaaa ccccacccct cactctgctt ctccccgcag gatgttcctg
     1261 tccttcccca ccaccaagac ctacttcccg cacttcgacc tgagccacgg ctctgcccag
     1321 gttaagggcc acggcaagaa ggtggccgac gcgctgacca acgccgtggc gcacgtggac
     1381 gacatgccca acgcgctgtc cgccctgagc gacctgcacg cgcacaagct tcgggtggac
     1441 ccggtcaact tcaaggtgag cggcgggccg ggagcgatct gggtcgaggg gcgagatggc
     1501 gccttcctcg cagggcagag gatcacgcgg gttgcgggag gtgtagcgca ggcggcggct
     1561 gcgggcctgg gccctcggcc ccactgaccc tcttctctgc acagctccta agccactgcc
     1621 tgctggtgac cctggccgcc cacctccccg ccgagttcac ccctgcggtg cacgcctccc
     1681 tggacaagtt cctggcttct gtgagcaccg tgctgacctc caaataccgt taagctcgct
     1741 ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc taagtccaac tactaaactg
     1801 ggggatatta tgaagggcct tgagcatctg gattctgcct aataaaaaac atttattttc
     1861 attgcggtct attttcccac ccttaggctg ctggtggtct acccttggac ccagaggttc
     1921 tttgagtcct ttggggatct gtccactcct gatgctgtta tgggcaaccc taaggtgaag
     1981 gctcatggca agaaagtgct cggtgccttt agtgatggcc tggctcacct ggacaacctc
     2041 aagggcacct ttgccacact gagtgagctg cactgtgaca agctgcacgt ggatcctgag
     2101 aacttcaggg tgagtctatg ggacgcttga tgttttcttt ccccttcttt tctatggtta
     2161 agttcatgtc ataggaaggg gataagtaac agggtacagt ttagaatggg aaacagacga
     2221 atgattgcat cagtgtggaa gtctcaggat cgttttagtt tcttttattt gctgttcata
     2281 acaattgttt tcttttgttt aattcttgct ttcttttttt ttcttctccg caatttttac
     2341 tattatactt aatgccttaa cattgtgtat aacaaaagga aatatctctg agatacatta
     2401 agtaacttaa aaaaaaactt tacacagtct gcctagtaca ttactatttg gaatatatgt
     2461 gtgcttattt gcatattcat aatctcccta ctttattttc ttttattttt aattgataca
     2521 taatcattat acatatttat gggttaaagt gtaatgtttt aatatgtgta cacatattga
     2581 ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact
     2641 tttttgttta tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat
     2701 acaatgtatc atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa
     2761 ggcaatGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG
     2821 CTCGCTCACT GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC
     2881 CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT GCAGGGGCGC CTGATGCGGT ATTTTCTCCT
     2941 TACGCATCTG TGCGGTATTT CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT
     3001 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
     3061 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
     3121 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
     3181 CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
     3241 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
     3301 CAAACTGGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG
     3361 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
     3421 AACAAAATAT TAACGTTTAC AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC
     3481 GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT
     3541 CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG
     3601 AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT
     3661 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA
     3721 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC
     3781 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT
     3841 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT
     3901 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT
     3961 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT
     4021 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC
     4081 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC
     4141 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT
     4201 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG
     4261 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG
     4321 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA
     4381 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA
     4441 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT
     4501 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC
     4561 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG
     4621 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT
     4681 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT
     4741 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC
     4801 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
     4861 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     4921 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
     4981 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
     5041 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
     5101 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
     5161 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
     5221 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA
     5281 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     5341 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     5401 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     5461 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GT
//