LOCUS       MKC106_HBB(HBA1-IRES-tE 7411 bp ds-DNA     circular     10-JAN-2025
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:Amplicon for qPCR, Amplicon for qPCR,
            AmpR, Right_AAV2 ITR, HBA1_5'UTR, HBA1_3'UTR"
FEATURES             Location/Qualifiers
     LTR             1..130
                     /label="Left-ITR_AAV"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    68..130
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    139..988
                     /label="LHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     source          889..988
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: organism: unspecified"
     misc_feature    989..1054
                     /label="HBA1_5'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1055..1149
                     /label="HBA1_Exon_1"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1267..1471
                     /label="HBA1_Exon_2"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1621..1746
                     /label="HBA1_Exon_3"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    1747..1749
                     /label="STOP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    1750..2324
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     misc_feature    2325..2327
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     misc_feature    2328..2336
                     /label="IRES2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)"
     misc_feature    2337..3653
                     /label="tEPOR_cDNA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3654..3764
                     /label="HBA1_3'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     source          3765..4664
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="mol_type: genomic DNA: organism: unspecified"
     misc_feature    3765..4664
                     /label="RHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3786..4008
                     /label="HBB_Exon_2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: HBB_Exon_2"
     misc_feature    4674..4736
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   4674..4814
                     /label="Right_AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      4889..5344
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        5626..5730
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             5731..5799
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             5800..6591
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      6762..7350
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAT TAGTCCAGGC AGAAACAGTT AGATGTCCCC AGTTAACCTC
      181 CTATTTGACA CCACTGATTA CCCCATTGAT AGTCACACTT TGGGTTGTAA GTGACTTTTT
      241 ATTTATTTGT ATTTTTGACT GCATTAAGAG GTCTCTAGTT TTTTATCTCT TGTTTCCCAA
      301 AACCTAATAA GTAACTAATG CACAGAGCAC ATTGATTTGT ATTTATTCTA TTTTTAGACA
      361 TAATTTATTA GCATGCATGA GCAAATTAAG AAAAACAACA ACAAATGAAT GCATATATAT
      421 GTATATGTAT GTGTGTATAT ATACACACAT ATATATATAT ATTTTTTCTT TTCTTACCAG
      481 AAGGTTTTAA TCCAAATAAG GAGAAGATAT GCTTAGAACC GAGGTAGAGT TTTCATCCAT
      541 TCTGTCCTGT AAGTATTTTG CATATTCTGG AGACGCAGGA AGAGATCCAT CTACATATCC
      601 CAAAGCTGAA TTATGGTAGA CAAAACTCTT CCACTTTTAG TGCATCAACT TCTTATTTGT
      661 GTAATAAGAA AATTGGGAAA ACGATCTTCA ATATGCTTAC CAAGCTGTGA TTCCAAATAT
      721 TACGTAAATA CACTTGCAAA GGAGGATGTT TTTAGTAGCA ATTTGTACTG ATGGTATGGG
      781 GCCAAGAGAT ATATCTTAGA GGGAGGGCTG AGGGTTTGAA GTCCAACTCC TAAGCCAGTG
      841 CCAGAAGAGC CAAGGACAGG TACGGCTGTC ATCACTTAGA CCTCACCCtg tggagccaca
      901 ccctagggtt ggccaatcta ctcccaggag cagggagggc aggagccagg gctgggcata
      961 aaagtcaggg cagagccatc tattgcttca taaaccctgg cgcgctcgcg gcccggcact
     1021 cttctggtcc ccacagactc agagagaacc caccatggtg ctgtctcctg ccgacaagac
     1081 caacgtcaag gccgcctggg gtaaggtcgg cgcgcacgct ggcgagtatg gtgcggaggc
     1141 cctggagagg tgaggctccc tcccctgctc cgacccgggc tcctcgcccg cccggaccca
     1201 caggccaccc tcaaccgtcc tggccccgga cccaaacccc acccctcact ctgcttctcc
     1261 ccgcaggatg ttcctgtcct tccccaccac caagacctac ttcccgcact tcgacctgag
     1321 ccacggctct gcccaggtta agggccacgg caagaaggtg gccgacgcgc tgaccaacgc
     1381 cgtggcgcac gtggacgaca tgcccaacgc gctgtccgcc ctgagcgacc tgcacgcgca
     1441 caagcttcgg gtggacccgg tcaacttcaa ggtgagcggc gggccgggag cgatctgggt
     1501 cgaggggcga gatggcgcct tcctcgcagg gcagaggatc acgcgggttg cgggaggtgt
     1561 agcgcaggcg gcggctgcgg gcctgggccc tcggccccac tgaccctctt ctctgcacag
     1621 ctcctaagcc actgcctgct ggtgaccctg gccgcccacc tccccgccga gttcacccct
     1681 gcggtgcacg cctccctgga caagttcctg gcttctgtga gcaccgtgct gacctccaaa
     1741 taccgtTGAc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     1801 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     1861 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     1921 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     1981 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2041 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     2101 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     2161 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     2221 gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     2281 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccATGG
     2341 ACCACCTCGG GGCGTCCCTC TGGCCCCAGG TCGGCTCCCT TTGTCTCCTG CTCGCTGGGG
     2401 CCGCCTGGGC GCCCCCGCCT AACCTCCCGG ACCCCAAGTT CGAGAGCAAA GCGGCCTTGC
     2461 TGGCGGCCCG GGGGCCCGAA GAGCTTCTGT GCTTCACCGA GCGGTTGGAG GACTTGGTGT
     2521 GTTTCTGGGA GGAAGCGGCG AGCGCTGGGG TGGGCCCGGG CAACTACAGC TTCTCCTACC
     2581 AGCTCGAGGA TGAGCCATGG AAGCTGTGTC GCCTGCACCA GGCTCCCACG GCTCGTGGTG
     2641 CGGTGCGCTT CTGGTGTTCG CTGCCTACAG CCGACACGTC GAGCTTCGTG CCCCTAGAGT
     2701 TGCGCGTCAC AGCAGCCTCC GGCGCTCCGC GATATCACCG TGTCATCCAC ATCAATGAAG
     2761 TAGTGCTCCT AGACGCCCCC GTGGGGCTGG TGGCGCGGTT GGCTGACGAG AGCGGCCACG
     2821 TAGTGTTGCG CTGGCTCCCG CCGCCTGAGA CACCCATGAC GTCTCACATC CGCTACGAGG
     2881 TGGACGTCTC GGCCGGCAAC GGCGCAGGGA GCGTACAGAG GGTGGAGATC CTGGAGGGCC
     2941 GCACCGAGTG TGTGCTGAGC AACCTGCGGG GCCGGACGCG CTACACCTTC GCCGTCCGCG
     3001 CGCGTATGGC TGAGCCGAGC TTCGGCGGCT TCTGGAGCGC CTGGTCGGAG CCTGTGTCGC
     3061 TGCTGACGCC TAGCGACCTG GACCCCCTCA TCCTGACGCT CTCCCTCATC CTCGTGGTCA
     3121 TCCTGGTGCT GCTGACCGTG CTCGCGCTGC TCTCCCACCG CCGGGCTCTG AAGCAGAAGA
     3181 TCTGGCCTGG CATCCCGAGC CCAGAGAGCG AGTTTGAAGG CCTCTTCACC ACCCACAAGG
     3241 GTAACTTCCA GCTGTGGCTG TACCAGAATG ATGGCTGCCT GTGGTGGAGC CCCTGCACCC
     3301 CCTTCACGGA GGACCCACCT GCTTCCCTGG AAGTCCTCTC AGAGCGCTGC TGGGGGACGA
     3361 TGCAGGCAGT GGAGCCGGGG ACAGATGATG AGGGCCCCCT GCTGGAGCCA GTGGGCAGTG
     3421 AGCATGCCCA GGATACCTAT CTGGTGCTGG ACAAATGGTT GCTGCCCCGG AACCCGCCCA
     3481 GTGAGGACCT CCCAGGGCCT GGTGGCAGTG TGGACATAGT GGCCATGGAT GAAGGCTCAG
     3541 AAGCATCCTC CTGCTCATCT GCTTTGGCCT CGAAGCCCAG CCCAGAGGGA GCCTCTGCTG
     3601 CCAGCTTTGA GTACACTATC CTGGACCCCA GCTCCCAGCT CTTGCGTCCA TGAgctggag
     3661 cctcggtggc catgcttctt gccccttggg cctcccccca gcccctcctc cccttcctgc
     3721 acccgtaccc ccgtggtctt tgaataaagt ctgagtgggc ggcaggtcta ttttcccacc
     3781 cttaggctgc tggtggtcta cccttggacc cagaggttct ttgagtcctt tggggatctg
     3841 tccactcctg atgctgttat gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc
     3901 ggtgccttta gtgatggcct ggctcacctg gacaacctca agggcacctt tgccacactg
     3961 agtgagctgc actgtgacaa gctgcacgtg gatcctgaga acttcagggt gagtctatgg
     4021 gacgcttgat gttttctttc cccttctttt ctatggttaa gttcatgtca taggaagggg
     4081 ataagtaaca gggtacagtt tagaatggga aacagacgaa tgattgcatc agtgtggaag
     4141 tctcaggatc gttttagttt cttttatttg ctgttcataa caattgtttt cttttgttta
     4201 attcttgctt tctttttttt tcttctccgc aatttttact attatactta atgccttaac
     4261 attgtgtata acaaaaggaa atatctctga gatacattaa gtaacttaaa aaaaaacttt
     4321 acacagtctg cctagtacat tactatttgg aatatatgtg tgcttatttg catattcata
     4381 atctccctac tttattttct tttattttta attgatacat aatcattata catatttatg
     4441 ggttaaagtg taatgtttta atatgtgtac acatattgac caaatcaggg taattttgca
     4501 tttgtaattt taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta
     4561 atactttccc taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg
     4621 caccattcta aagaataaca gtgataattt ctgggttaag gcaatGCGGC CGCAGGAACC
     4681 CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG
     4741 ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG
     4801 CAGCTGCCTG CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
     4861 ACACCGCATA CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG
     4921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT
     4981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
     5041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT
     5101 GATTTGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG
     5161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC
     5221 CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA
     5281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA
     5341 ATTTTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA
     5401 CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC
     5461 AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
     5521 AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA
     5581 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT
     5641 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA
     5701 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT
     5761 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA
     5821 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC
     5881 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT
     5941 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT
     6001 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT
     6061 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC
     6121 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG
     6181 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC
     6241 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA
     6301 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG
     6361 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT
     6421 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT
     6481 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA
     6541 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC
     6601 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC
     6661 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC
     6721 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG
     6781 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
     6841 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
     6901 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
     6961 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
     7021 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
     7081 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
     7141 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
     7201 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC
     7261 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
     7321 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
     7381 CTGGCCTTTT GCTGGCCTTT TGCTCACATG T
//