LOCUS       MKC131_CCR5(SFFV-synEPO 6242 bp ds-DNA     circular     10-JAN-2025
DEFINITION  .
KEYWORDS    "creator:Stanford University" "marker:AmpR, Amplicon for qPCR,
            Right_AAV2 ITR, Amplicon for qPCR, synEPOR"
FEATURES             Location/Qualifiers
     LTR             1..130
                     /label="Left-ITR_AAV"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_fwdcolor="#000080"
     misc_feature    68..130
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    139..538
                     /label="LHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    559..1050
                     /label="SFFV"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: SFFV promoter"
     sig_peptide     1084..1164
                     /label="Signal peptide - IL6"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="codon_start: 1 transl_table: 1 translation: MNSFSTSAFGPVAFSLGLLLVLPAAFP,AP"
     misc_feature    1084..2097
                     /label="synEPOR"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     sig_peptide     1165..1170
                     /label="Signal peptide - IL6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="codon_start: 1 transl_table: 1 translation: MNSFSTSAFGPVAFSLGLLLVLPAAFP,AP"
     misc_feature    1171..1185
                     /label="GGGGS_linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1186..1194
                     /label="FKBP-V"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: FKBP-V"
     misc_feature    1186..1515
                     /label="Fragment c"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: Fragment c"
     CDS             1195..1515
                     /label="FKBP-V"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="label: FKBP-V"
     misc_feature    1300..1302
                     /label="F36L_Barrero_2016_incs_spontaneous_dimerization"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1516..1530
                     /label="divGGGGS_linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1531..1533
                     /label="Uniprot_EPOR_ECdomain"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1534..1602
                     /label="Uniprot_EPOR_TMdomain"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1603..2097
                     /label="Uniprot_EPOR_ICdomain"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2098..2151
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. product: 2A peptide from Thosea asigna virus capsid protein protein_id: transl_table: 1 translation: EGRGSLLTCGDVEENPGP"
     CDS             2152..2154
                     /label="mEYFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002) transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     CDS             2155..2157
                     /label="mEYFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002) transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     CDS             2158..2871
                     /label="mEYFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002) transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     polyA_signal    2872..3096
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
                     /note="note: bovine growth hormone polyadenylation signal"
     misc_feature    3097..3496
                     /label="RHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3505..3567
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     repeat_region   3505..3645
                     /label="Right_AAV2 ITR"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_fwdcolor="#000080"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2"
     rep_origin      3720..4175
                     /label="f1 ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        4457..4561
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_fwdcolor="#800000"
                     /note="gene: bla"
     CDS             4562..5422
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: beta-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      5593..6181
                     /label="ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcAA GAGAGTTAAT TCAATGTAGA CATCTATGTA GGCAATTAAA
      181 AACCTATTGA TGTATAAAAC AGTTTGCATT CATGGAGGGC AACTAAATAC ATTCTAGGAC
      241 TTTATAAAAG ATCACTTTTT ATTTATGCAC AGGGTGGAAC AAGATGGATT ATCAAGTGTC
      301 AAGTCCAATC TATGACATCA ATTATTATAC ATCGGAGCCC TGCCAAAAAA TCAATGTGAA
      361 GCAAATCGCA GCCCGCCTCC TGCCTCCGCT CTACTCACTG GTGTTCATCT TTGGTTTTGT
      421 GGGCAACATG CTGGTCATCC TCATCCTGAT AAACTGCAAA AGGCTGAAGA GCATGACTGA
      481 CATCTACCTG CTCAACCTGG CCATCTCTGA CCTGTTTTTC CTTCTTACTG TCCCCTTCat
      541 taccctgtta tccctaggcc gataaaataa aagattttat ttagtctcca gaaaaagggg
      601 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
      661 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
      721 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
      781 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
      841 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
      901 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
      961 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     1021 ccagtcctcc gacagactga gtcgcccggg ggtaccgagc tcttcgaagg atccatcgcc
     1081 accATGAACT CCTTCTCCAC AAGCGCCTTC GGTCCAGTTG CCTTCTCCCT GGGGCTGCTC
     1141 CTGGTGTTGC CTGCTGCCTT CCCTGCCCCA GGcGGcGGcG GaAGcATGCT TGAGGGAGTG
     1201 CAGGTGGAAA CCATCTCCCC AGGAGACGGG CGCACCTTCC CCAAGCGCGG CCAGACCTGC
     1261 GTGGTGCACT ACACCGGGAT GCTTGAAGAT GGAAAGAAAG TTGATTCCTC CCGGGACAGA
     1321 AACAAGCCCT TTAAGTTTAT GCTAGGCAAG CAGGAGGTGA TCCGAGGCTG GGAAGAAGGG
     1381 GTTGCCCAGA TGAGTGTGGG TCAGAGAGCC AAACTGACTA TATCTCCAGA TTATGCCTAT
     1441 GGTGCCACTG GGCACCCAGG CATCATCCCA CCACATGCCA CTCTCGTCTT CGATGTGGAG
     1501 CTTCTAAAAC TGGAAGGAGG AGGAGGTAGT CCCCTCATCC TGACGCTCTC CCTCATCCTC
     1561 GTGGTCATCC TGGTGCTGCT GACCGTGCTC GCGCTGCTCT CCCACCGCCG GGCTCTGAAG
     1621 CAGAAGATCT GGCCTGGCAT CCCGAGCCCA GAGAGCGAGT TTGAAGGCCT CTTCACCACC
     1681 CACAAGGGTA ACTTCCAGCT GTGGCTGTAC CAGAATGATG GCTGCCTGTG GTGGAGCCCC
     1741 TGCACCCCCT TCACGGAGGA CCCACCTGCT TCCCTGGAAG TCCTCTCAGA GCGCTGCTGG
     1801 GGGACGATGC AGGCAGTGGA GCCGGGGACA GATGATGAGG GCCCCCTGCT GGAGCCAGTG
     1861 GGCAGTGAGC ATGCCCAGGA TACCTATCTG GTGCTGGACA AATGGTTGCT GCCCCGGAAC
     1921 CCGCCCAGTG AGGACCTCCC AGGGCCTGGT GGCAGTGTGG ACATAGTGGC CATGGATGAA
     1981 GGCTCAGAAG CATCCTCCTG CTCATCTGCT TTGGCCTCGA AGCCCAGCCC AGAGGGAGCC
     2041 TCTGCTGCCA GCTTTGAGTA CACTATCCTG GACCCCAGCT CCCAGCTCTT GCGTCCAgag
     2101 ggcaggggca gcctgctgac ctgcggcgac gtggaggaga accccggccc catggtgagc
     2161 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2221 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2281 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2341 accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     2401 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2461 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2521 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2581 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2641 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2701 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2761 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2821 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta actgtgcctt
     2881 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2941 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3001 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3061 atagcaggca tgctggggat gcggtgggct ctatggtggg ctcactatgc tgccgcccag
     3121 tgggactttg gaaatacaat gtgtcaactc ttgacagggc tctattttat aggcttcttc
     3181 tctggaatct tcttcatcat cctcctgaca atcgataggt acctggctgt cgtccatgct
     3241 gtgtttgctt taaaagccag gacggtcacc tttggggtgg tgacaagtgt gatcacttgg
     3301 gtggtggctg tgtttgcgtc tctcccagga atcatcttta ccagatctca aaaagaaggt
     3361 cttcattaca cctgcagctc tcattttcca tacagtcagt atcaattctg gaagaatttc
     3421 cagacattaa agatagtcat cttggggctg gtcctgccgc tgcttgtcat ggtcatctgc
     3481 tactcgggaa tcctaagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     3541 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     3601 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     3661 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     3721 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3781 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3841 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3901 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     3961 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4021 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     4081 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4141 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4201 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4261 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4321 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4381 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4441 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4501 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4561 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4621 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4681 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4741 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4801 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4861 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4921 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4981 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5041 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5101 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5161 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5221 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5281 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5341 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5401 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5461 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5521 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5581 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5641 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5701 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5761 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5821 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5881 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5941 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6001 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6061 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6121 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6181 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6241 gt
//